997 resultados para Physiology, Comparative.


Relevância:

80.00% 80.00%

Publicador:

Resumo:

Mode of access: Internet.

Relevância:

80.00% 80.00%

Publicador:

Resumo:

Includes bibliographies.

Relevância:

70.00% 70.00%

Publicador:

Resumo:

We undertook deep sequencing of gill transcriptomes from two freshwater crayfish, Cherax cainii and Cherax destructor, in order to generate genomic resources for future genomics research. Over 83 and 100 million high quality (quality score (Q) ≥ 30) paired-end Illumina reads (150 bp) were assembled into 147,101 and 136,622 contigs in C. cainii and C. destructor, respectively. A total of 24,630 and 23,623 contigs received significant BLASTx hits and allowed the identification of multiple gill expressed candidate genes associated with pH and salinity balance. These functionally annotated transcripts will provide a resource to facilitate comparative genomic research in the genus Cherax, and in particular allow insights into respiratory and osmoregulatory physiology of this group of animals.

Relevância:

70.00% 70.00%

Publicador:

Resumo:

Experiments were performed to determine whether capacitative Ca(2+) entry (CCE) can be activated in canine pulmonary and renal arterial smooth muscle cells (ASMCs) and whether activation of CCE parallels the different functional structure of the sarcoplasmic reticulum (SR) in these two cell types. The cytosolic [Ca(2+)] was measured by imaging fura-2-loaded individual cells. Increases in the cytosolic [Ca(2+)] due to store depletion in pulmonary ASMCs required simultaneous depletion of both the inositol 1,4,5-trisphosphate (InsP(3))- and ryanodine (RY)-sensitive SR Ca(2+) stores. In contrast, the cytosolic [Ca(2+)] rises in renal ASMCs occurred when the SR stores were depleted through either the InsP(3) or RY pathways. The increase in the cytosolic [Ca(2+)] due to store depletion in both pulmonary and renal ASMCs was present in cells that were voltage clamped and was abolished when cells were perfused with a Ca(2+)-free bathing solution. Rapid quenching of the fura-2 signal by 100 microM Mn(2+) following SR store depletion indicated that extracellular Ca(2+) entry increased in both cell types and also verified that activation of CCE in pulmonary ASMCs required the simultaneous depletion of the InsP(3)- and RY-sensitive SR Ca(2+) stores, while CCE could be activated in renal ASMCs by the depletion of either of the InsP(3)- or RY-sensitive SR stores. Store depletion Ca(2+) entry in both pulmonary and renal ASMCs was strongly inhibited by Ni(2+) (0.1-10 mM), slightly inhibited by Cd(2+) (200-500 microM), but was not significantly affected by the voltage-gated Ca(2+) channel (VGCC) blocker nisoldipine (10 microM). The non-selective cation channel blocker Gd(3+) (100 microM) inhibited a portion of the Ca(2+) entry in 6 of 18 renal but not pulmonary ASMCs. These results provide evidence that SR Ca(2+) store depletion activates CCE in parallel with the organization of intracellular Ca(2+) stores in canine pulmonary and renal ASMCs.

Relevância:

70.00% 70.00%

Publicador:

Resumo:

Cover title: Veterinary physiology.

Relevância:

70.00% 70.00%

Publicador:

Resumo:

Includes index.

Relevância:

70.00% 70.00%

Publicador:

Resumo:

Mode of access: Internet.

Relevância:

70.00% 70.00%

Publicador:

Resumo:

Mode of access: Internet.

Relevância:

60.00% 60.00%

Publicador:

Resumo:

"Responsio ad quaestionem zoologicam, a nobilissimo ordine doctrinarum physicarum et mathematicarum in Academia Lugduno-Batava"--Page following title page

Relevância:

60.00% 60.00%

Publicador:

Resumo:

Mode of access: Internet.

Relevância:

60.00% 60.00%

Publicador:

Resumo:

"Verzeichnis der Litteratur," i. 91-93.