Redes sociais e democracia deliberativa

Este artigo pretende conectar aspectos gerais das teorias deliberativas de democracia a mecanismos específicos disponíveis aos usuários das redes sociais. Aponta-se quais ferramentas das redes sociais colaboram com a constituição de uma esfera pública e o desenvolvimento de uma democracia deliberativa, especialmente quando incorporadas pela comunicação do Estado. Para tanto, reconstruímos sistematicamente a estrutura teórica da democracia deliberativa e, em seguida, avaliamos ferramentas específicas, ilustrando-as com exemplos nacionais de seu uso. Julgamos que esse tipo de análise é relevante para que se determine no futuro como estruturar a comunicação entre órgãos estatais e sociedade, de maneira a maximizar seu potencial deliberativo.

O Punk não é só para o seu Namorado: esfera pública alternativa, processos de identificação e testemunho na cena musical Riot Grrrl

The Riot Grrrl movement started in mid-90s and it consists of girls who use rock as a tool of feminist political struggle. Besides using music as a central element of political identity, this movement is also characterized by the formation of an alternative public sphere (formed by fanzines, blogs and e-zines) that functions both as a way to spread their music and as identification trigger mechanism in relation to their political causes. The aim of this article is to study the instruments that these feminists bands - that deviate from the traditional feminist political movements - use to create identification with their audience, especially from an empirical research of e-zines published by these bands and from the communicative action of their leaders in social networks. It is possible to note that, despite other identification mechanisms, testimonies works as a socialization link which marks the alternative public sphere composed by publications made by Riot Grrrls. The testimony serves as a powerful arranger of collective identities, since it is presupposed in the recognition of a world in common, allocating identity as performative action.

Séneca, didascalias y voluntad de representación

De las múltiples problemáticas planteadas por la obra trágica de Séneca, una de las que más polémica causa es la cuestión de la representación de sus obras. Numerosos y admirados eruditos han debatido hasta el cansancio sobre este tema, a menudo utilizando los mismos argumentos para tesis encontradas (Pociña, Andrés, 'Una vez más sobre la representación de las tragedias de Séneca', Emerita 41, 1973, 297-308.). En este trabajo intentamos corremos de esta discusión histórico-arqueológica para enfocamos en el entramado textual mismo en busca de pasajes claramente performativos (meso y microdidascalias textuales) que revelan una 'voluntad de representación', al menos primigenia, por parte del autor, más allá de si las obras fueron representadas o no alguna vez. El teatro antiguo tiene por convención la enunciación efectiva de las acciones escénicas y,a la vez, toda acción performativa tiene su correlato en el discurso. Estos pasajes cumplen la función de didascalias internas y responden a una voluntad del autor de influir en la representación (Issacharof, Michael, 'Voix, autorité, didascalies', Poétique 96, 1993, 463-474). La presencia de meso y microdidascalias, relevantes y fundamentales para el desarrollo dramático y trágico, son la huella rastreable de que existió una 'voluntad de representación' por parte de Séneca, al menos en el momento de creación y concepción poética de sus tragedias

Séneca, didascalias y voluntad de representación

De las múltiples problemáticas planteadas por la obra trágica de Séneca, una de las que más polémica causa es la cuestión de la representación de sus obras. Numerosos y admirados eruditos han debatido hasta el cansancio sobre este tema, a menudo utilizando los mismos argumentos para tesis encontradas (Pociña, Andrés, 'Una vez más sobre la representación de las tragedias de Séneca', Emerita 41, 1973, 297-308.). En este trabajo intentamos corremos de esta discusión histórico-arqueológica para enfocamos en el entramado textual mismo en busca de pasajes claramente performativos (meso y microdidascalias textuales) que revelan una 'voluntad de representación', al menos primigenia, por parte del autor, más allá de si las obras fueron representadas o no alguna vez. El teatro antiguo tiene por convención la enunciación efectiva de las acciones escénicas y,a la vez, toda acción performativa tiene su correlato en el discurso. Estos pasajes cumplen la función de didascalias internas y responden a una voluntad del autor de influir en la representación (Issacharof, Michael, 'Voix, autorité, didascalies', Poétique 96, 1993, 463-474). La presencia de meso y microdidascalias, relevantes y fundamentales para el desarrollo dramático y trágico, son la huella rastreable de que existió una 'voluntad de representación' por parte de Séneca, al menos en el momento de creación y concepción poética de sus tragedias

Séneca, didascalias y voluntad de representación

De las múltiples problemáticas planteadas por la obra trágica de Séneca, una de las que más polémica causa es la cuestión de la representación de sus obras. Numerosos y admirados eruditos han debatido hasta el cansancio sobre este tema, a menudo utilizando los mismos argumentos para tesis encontradas (Pociña, Andrés, 'Una vez más sobre la representación de las tragedias de Séneca', Emerita 41, 1973, 297-308.). En este trabajo intentamos corremos de esta discusión histórico-arqueológica para enfocamos en el entramado textual mismo en busca de pasajes claramente performativos (meso y microdidascalias textuales) que revelan una 'voluntad de representación', al menos primigenia, por parte del autor, más allá de si las obras fueron representadas o no alguna vez. El teatro antiguo tiene por convención la enunciación efectiva de las acciones escénicas y,a la vez, toda acción performativa tiene su correlato en el discurso. Estos pasajes cumplen la función de didascalias internas y responden a una voluntad del autor de influir en la representación (Issacharof, Michael, 'Voix, autorité, didascalies', Poétique 96, 1993, 463-474). La presencia de meso y microdidascalias, relevantes y fundamentales para el desarrollo dramático y trágico, son la huella rastreable de que existió una 'voluntad de representación' por parte de Séneca, al menos en el momento de creación y concepción poética de sus tragedias

L’empreinte d’une expérience performative en littérature : le cas de Sophie Calle et de Miranda July

Le présent mémoire propose de croiser les démarches de deux auteures et artistes contemporaines, Sophie Calle et Miranda July, dont les quatre œuvres à l’étude – Douleur exquise (2003), Aveugles (2011), Rachel, Monique (2012) de Calle et Il vous choisit (2013) de July – se fondent sur des expériences en amont de l’écriture qui mobilisent le corps même des auteures, les engagent dans une action concrète et, bien souvent, dans des interactions avec autrui. Cet art de la contrainte, cet art action qui devient le sédiment de leurs écrits s’inscrit dans la filiation hypothétique des théories du philosophe pragmatique John Dewey et de celles de l’artiste Allan Kaprow – l’un des premiers à réfléchir l’art de la performance. L’écriture intermédiale qu’elles pratiquent – ce jeu de relations entre différents médias au sein même de l’œuvre – permet à la fois de réactiver la valeur performative de l’expérience qui a impulsé la création littéraire et d’embrayer une expérience de lecture qui devient elle-même performative. Exemplaires d’une esthétique relationnelle, polyphoniques dans les voix qui s’expriment, les quatre ouvrages du corpus donnent à sentir le bruissement d’une communauté. Il s’agit d’une littérature interdisciplinaire et intersubjective, mais surtout performative dans son questionnement incessant sur le pouvoir de l’art pour transformer la vie.

L’empreinte d’une expérience performative en littérature : le cas de Sophie Calle et de Miranda July

Le présent mémoire propose de croiser les démarches de deux auteures et artistes contemporaines, Sophie Calle et Miranda July, dont les quatre œuvres à l’étude – Douleur exquise (2003), Aveugles (2011), Rachel, Monique (2012) de Calle et Il vous choisit (2013) de July – se fondent sur des expériences en amont de l’écriture qui mobilisent le corps même des auteures, les engagent dans une action concrète et, bien souvent, dans des interactions avec autrui. Cet art de la contrainte, cet art action qui devient le sédiment de leurs écrits s’inscrit dans la filiation hypothétique des théories du philosophe pragmatique John Dewey et de celles de l’artiste Allan Kaprow – l’un des premiers à réfléchir l’art de la performance. L’écriture intermédiale qu’elles pratiquent – ce jeu de relations entre différents médias au sein même de l’œuvre – permet à la fois de réactiver la valeur performative de l’expérience qui a impulsé la création littéraire et d’embrayer une expérience de lecture qui devient elle-même performative. Exemplaires d’une esthétique relationnelle, polyphoniques dans les voix qui s’expriment, les quatre ouvrages du corpus donnent à sentir le bruissement d’une communauté. Il s’agit d’une littérature interdisciplinaire et intersubjective, mais surtout performative dans son questionnement incessant sur le pouvoir de l’art pour transformer la vie.

Direct Visualization Of The Action Of Triton X-100 On Giant Vesicles Of Erythrocyte Membrane Lipids.

The raft hypothesis proposes that microdomains enriched in sphingolipids, cholesterol, and specific proteins are transiently formed to accomplish important cellular tasks. Equivocally, detergent-resistant membranes were initially assumed to be identical to membrane rafts, because of similarities between their compositions. In fact, the impact of detergents in membrane organization is still controversial. Here, we use phase contrast and fluorescence microscopy to observe giant unilamellar vesicles (GUVs) made of erythrocyte membrane lipids (erythro-GUVs) when exposed to the detergent Triton X-100 (TX-100). We clearly show that TX-100 has a restructuring action on biomembranes. Contact with TX-100 readily induces domain formation on the previously homogeneous membrane of erythro-GUVs at physiological and room temperatures. The shape and dynamics of the formed domains point to liquid-ordered/liquid-disordered (Lo/Ld) phase separation, typically found in raft-like ternary lipid mixtures. The Ld domains are then separated from the original vesicle and completely solubilized by TX-100. The insoluble vesicle left, in the Lo phase, represents around 2/3 of the original vesicle surface at room temperature and decreases to almost 1/2 at physiological temperature. This chain of events could be entirely reproduced with biomimetic GUVs of a simple ternary lipid mixture, 2:1:2 POPC/SM/chol (phosphatidylcholine/sphyngomyelin/cholesterol), showing that this behavior will arise because of fundamental physicochemical properties of simple lipid mixtures. This work provides direct visualization of TX-100-induced domain formation followed by selective (Ld phase) solubilization in a model system with a complex biological lipid composition.

Presynaptic Neuromuscular Action Of A Methanolic Extract From The Venom Of Rhinella Schneideri Toad.

Rhinella schneideri, previously known as Bufo paracnemis, is a common toad in many regions of Brazil. Its venom exerts important cardiovascular effects on humans and other animals. Although this toad venom has been the subject of intense investigations, little is known about its neuromuscular activity. The neurotoxicity of a methanolic extract of R. schneideri venom was tested on mouse phrenic nerve-diaphragm (PND) preparations mounted for conventional twitch tension recording - in response to indirect stimulation - and for electrophysiological measurements. Venom extract (50 μg/mL) increased the muscle twitch tension in PND preparations but did not significantly alter the resting membrane potential values. Electrophysiological evaluations showed that the extract (50 μg/mL) significantly augmented the frequency of miniature end-plate potential (from 38 ± 3.5 to 88 ± 15 after 60 minutes; n = 5; p < 0.05) and quantal content (from 128 ± 13 to 272 ± 34 after five minutes; n = 5; p < 0.05). Pretreatment with ouabain (1 μg/mL) for five minutes prevented the increase in quantal content (117 ± 18 and 154 ± 33 after five and 60 minutes, respectively). These results indicate that the methanolic extract of R. schneideri venom acts primarily presynaptically to enhance neurotransmitter release in mouse phrenic-diaphragm preparations.

Action Of Essential Oils From Brazilian Native And Exotic Medicinal Species On Oral Biofilms.

Essential oils (EO) obtained from twenty medicinal and aromatic plants were evaluated for their antimicrobial activity against the oral pathogens Candida albicans, Fusobacterium nucleatum, Porphyromonas gingivalis, Streptococcus sanguis and Streptococcus mitis. The antimicrobial activity of the EO was evaluates by microdilution method determining Minimal Inhibitory Concentration. Chemical analysis of the oils compounds was performed by Gas chromatography-mass spectrometry (CG-MS). The most active EO were also investigated as to their actions on the biolfilm formation. The most of the essential oils (EO) presented moderate to strong antimicrobial activity against the oral pathogens (MIC--Minimal Inhibitory Concentrations values between 0.007 and 1.00 mg/mL). The essential oil from Coriandrum sativum inhibited all oral species with MIC values from 0.007 to 0.250 mg/mL, and MBC/MFC (Minimal Bactericidal/Fungicidal Concentrations) from 0.015 to 0.500 mg/mL. On the other hand the essential oil of C. articulatus inhibited 63.96% of S. sanguis biofilm formation. Through Scanning Eletronic Microscopy (SEM) images no changes were observed in cell morphology, despite a decrease in biofilm formation and changes on biofilm structure. Chemical analysis by Gas Chromatography-Mass Spectrometry (GC-MS) of the C. sativum essential oil revealed major compounds derivatives from alcohols and aldehydes, while Cyperus articulatus and Aloysia gratissima (EOs) presented mono and sesquiterpenes. In conclusion, the crude oil from C. articulatus exhibited the best results of antimicrobial activity e ability to control biofilm formation. The chemical analysis showed the presence of terpenes and monoterpenes such as a-pinene, a-bulnesene and copaene. The reduction of biofilms formation was confirmed from SEM images. The results of this research shows a great potential from the plants studied as new antimicrobial sources.

[strategies For Tackling Absenteeism In Dental Appointments In The Family Health Units Of A Large Municipality: Action Research].

The aim of this study was to analyze the reasons for missed appointments in dental Family Health Units (FHU) and implement strategies to reduce same through action research. This is a study conducted in 12 FHUs in Piracicaba in the State of São Paulo from January, 1 to December, 31 2010. The sample was composed of 385 users of these health units who were interviewed over the phone and asked about the reasons for missing dental appointments, as well as 12 dentists and 12 nurses. Two workshops were staged with professionals: the first to assess the data collected in interviews and develop strategy, and the second for evaluation after 4 months. The primary cause for missed appointments was the opening hours of the units coinciding with the work schedule of the users. Among the strategies suggested were lectures on oral health, ongoing education in team meetings, training of Community Health Agents, participation in therapeutic groups and partnerships between Oral Health Teams and the social infrastructure of the community. The adoption of the single medical record was the strategy proposed by professionals. The strategies implemented led to a 66.6% reduction in missed appointments by the units and the motivating nature of the workshops elicited critical reflection to redirect health practices.

Nonquadratic gauge fixing and global gauge invariance in the effective action

The possibility of having a gauge fixing term in the effective Lagrangian that is not a quadratic expression has been explored in spin-two theories so as to have a propagator that is both traceless and transverse. We first show how this same approach can be used in spontaneously broken gauge theories as an alternate to the 't Hooft gauge fixing which avoids terms quadratic in the scalar fields. This ""nonquadratic"" gauge fixing in the effective action results in two complex fermionic and one real bosonic ghost field. A global gauge invariance involving a fermionic gauge parameter, analogous to the usual Becchi-Rouet-Stora-Tyutin invariance, is present in this effective action.

Ambiguities in the effective action in Lorentz-violating gravity

We investigate the occurrence of ambiguities for the Lorentz-violating gravitational Chern-Simons term. It turns out that this term is accompanied by a coefficient depending on an undetermined parameter, due to an arbitrariness in the choice of the conserved current.

Antimicrobial photodynamic action on dentin using a light-emitting diode light source

Objective: The aim of this study was the evaluation of two different photosensitizers activated by red light emitted by light-emitting diodes (LEDs) in the decontamination of carious bovine dentin. Materials and Methods: Fifteen bovine incisors were used to obtain dentin samples which were immersed in brain-heart infusion culture medium supplemented with 1% glucose, 2% sucrose, and 1% young primary culture of Lactobacillus acidophilus 108 CFU/mL and Streptococcus mutans 108 CFU/mL for caries induction. Three different concentrations of the Photogem solution, a hematoporphyrin derivative (1, 2, and 3 mg/mL) and two different concentrations of toluidine blue O (TBO), a basic dye (0.025 and 0.1 mg/mL) were used. To activate the photosensitizers two different light exposure times were used: 60 sec and 120 sec, corresponding respectively to the doses of 24 J/cm(2) and 48 J/cm(2). Results: After counting the numbers of CFU per milligram of carious dentin, we observed that the use of LED energy in association with Photogem or TBO was effective for bacterial reduction in carious dentin, and that the greatest effect on S. mutans and L. acidophilus was obtained with TBO at 0.1 mg/mL and a dose of 48 J/cm(2). It was also observed that the overall toxicity of TBO was higher than that of Photogem, and that the phototoxicity of TBO was higher than that of Photogem. Conclusion: Based on our data we propose a mathematical model for the photodynamic effect when different photosensitizer concentrations and light doses are used.

Bothrops jararaca Peptide with Anti-Hypertensive Action Normalizes Endothelium Dysfunction Involved in Physiopathology of Preeclampsia

Preeclampsia, a pregnancy-specific syndrome characterized by hypertension, proteinuria and edema, is a major cause of fetal and maternal morbidity and mortality especially in developing countries. Bj-PRO-10c, a proline-rich peptide isolated from Bothrops jararaca venom, has been attributed with potent anti-hypertensive effects. Recently, we have shown that Bj-PRO-10c-induced anti-hypertensive actions involved NO production in spontaneous hypertensive rats. Using in vitro studies we now show that Bj-PRO-10c was able to increase NO production in human umbilical vein endothelial cells from hypertensive pregnant women (HUVEC-PE) to levels observed in HUVEC of normotensive women. Moreover, in the presence of the peptide, eNOS expression as well as argininosuccinate synthase activity, the key rate-limiting enzyme of the citrulline-NO cycle, were enhanced. In addition, excessive superoxide production due to NO deficiency, one of the major deleterious effects of the disease, was inhibited by Bj-PRO-10c. Bj-PRO-10c induced intracellular calcium fluxes in both, HUVEC-PE and HUVEC, which, however, led to activation of eNOS expression only in HUVEC-PE. Since Bj-PRO-10c promoted biological effects in HUVEC from patients suffering from the disorder and not in normotensive pregnant women, we hypothesize that Bj-PRO-10c induces its anti-hypertensive effect in mothers with preeclampsia. Such properties may initiate the development of novel therapeutics for treating preeclampsia.