Host-parasite-environment relationship, morphology and molecular analyses of Henneguya eirasi n. sp parasite of two wild Pseudoplatystoma spp. in Pantanal Wetland, Brazil

A new myxosporean species, Henneguya eirasi n. sp., is described parasitizing the gill filaments of Pseudoplatystoma corruscans and Pseudoplatystoma fasciatum (Siluriformes: Pimelodidae) caught in the Patanal Wetland of the state of Mato Grosso, Brazil. The parasite formed white, elongated plasmodia measuring up to 3 mm. Mature spores were ellipsoidal in the frontal view, measuring 37.1 +/- 1.8 mu m in total length, 12.9 +/- 0.8 mu m in body length, 3.4 +/- 0.3 mu m in width, 3.1 +/- 0.1 mu m in thickness and 24.6 +/- 2.2 mu m in the caudal process. Polar capsules were elongated and equal in size, measuring 5.4 +/- 0.5 mu m in length and 0.7 +/- 0.1 mu m in width. Polar filaments had 12-13 coils. Histopathological analysis revealed that the parasite developed in the sub-epithelial connective tissue of the gill filaments and the plasmodia were surrounded by a capsule of host connective tissue. The plasmodia caused slight compression of the adjacent tissues, but no inflammatory response was observed in the infection site. Ultrastructure analysis revealed a single plasmodial wall connected to the ectoplasmic zone through numerous pinocytotic canals. The plasmodial wall exhibited numerous projections and slightly electron-dense material was found in the ectoplasm next to the plasmodial wall, forming a line just below the wall. Partial sequencing of the 18S rDNA gene of H. eirasi n. sp. obtained from P. fasciatum resulted in a total of 1066 bp and this sequence did not match any of the Myxozoa available in the GenBank. Phylogenetic analysis revealed the Henneguya species clustering into clades following the order and family of the host fishes. H. eirasi n. sp. clustered alone in one clade, which was the basal unit for the clade composed of Henneguya species parasites of siluriform ictalurids. The prevalence of the parasite was 17.1% in both fish species examined. Parasite prevalence was not influenced by season, host sex or host size. (C) 2011 Elsevier B.V. All rights reserved.

First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil

O objetivo do presente estudo foi reportar a infecção por Trypanosoma sp. em tuviras (Gymnotus aff. inaequilabiatus) oriundas do Pantanal Sul-mato-grossense, Brasil. Dez peixes provenientes do rio Paraguai, Pantanal Sul-mato-grossense, foram avaliados quanto à presença de hemoflagelados. Tripomastigotas de Trypanosoma sp. foram observados nas extensões sanguíneas de três peixes (30% de prevalência), e algumas formas encontravam-se em divisão. Por meio do exame a fresco e da centrifugação do sangue em capilar de hematócrito como métodos para diagnóstico, a taxa de prevalência foi de 80%. Esse é o primeiro relato de Trypanosoma sp. em tuviras no Brasil.

Phylogeny, ultrastructure, histopathology and prevalence of Myxobolus oliveirai sp. nov., a parasite of Brycon hilarii (Characidae) in the Pantanal wetland, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

River avulsions on the Taquari megafan, Pantanal wetland, Brazil

The Pantanal wetland is located in a tectonically active interior sedimentary basin in west-central Brazil. The south-flowing Paraguay River is the trunk-river of an alluvial constructional landform comprising several large alluvial fans, the largest one of which is the Taquari megafan. The Taquari River flows in two distinct geomorphologic zones within the megafan. Entrenched on sediments of Pleistocene fan lobes, the Taquari River flows in a 3 to 5 km wide meander belt in the upper fan, where avulsion is hindered by entrenchment. Downstream of the intersection point, stream discharge progressively decreases and the Taquari River becomes narrow and shallow toward the Paraguay River plain. Within the distributary fan lobe, the channel-levee sandy complex is topographically higher than the adjacent floodplains and avulsion is a natural consequence of crevasses in the natural levees. Many channel avulsions have occurred during the last decades and documented cases show that significant channel changes may take place in a few years. Beginning with crevassing in 1988 and ending with the abandonment of the former channel in 1998, the river completely changed course in the lower fan. Presently, a major avulsion is occurring in the upper portion of the growing fan lobe, where many crevasses have appeared in the natural levees with associated splays onto the floodbasin. New anastomosed channels have formed north of the Taquari River, but downstream of them the flow is unconfined and the water spreads into natural floodbasins. This avulsion is still in process and allows observation of channel evolution, the geomorphic features produced, the sedimentary processes involved, and resulting effects. If the new channels do not rejoin the main channel, the river mouth may abandon its present master channel and shift to a position a hundred kilometers north from its present position. A large volume of sediment has been transferred to the floodbasin, with progradation of crevasse splay deposits over fine overbank sediments. Many geomorphic features, recognizable in satellite and radar images, clearly show that avulsion has occurred many limes before in the Taquari River. Avulsion belt deposits and former diverted channels testify to ancient avulsion events within the fan lobe and show that progradation of splays onto the floodbasin is the most important infilling process within the Taquari distributary fan lobe. The avulsion process in the lower Taquari River has accelerated in the last 30 years, along with the magnitude of flooding. Pasture and intensive agriculture in the catchment area has increased the sediment supply to the wetland, but larger floods are also a consequence of higher rainfall since 1973. Avulsion and floods have been a cause of great concern among the local population and landowners. Before human intervention in attempting to control floods, however, a better understanding of the avulsive river system is needed, especially because a major navigation project including the channelization of the Paraguay River was recently proposed. (c) 2005 Elsevier B.V. All rights reserved.

Contrasting fluvial styles of the Paraguay River in the northwestern border of the Pantanal wetland, Brazil

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Physicochemical features of rivers and lakes in pantanal wetland

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

GPR survey in the Taquari River, Pantanal Wetland, west-central Brazil

A pioneer GPR - Ground Penetrating Radar - survey was carried out in the Pantanal of Mato Grosso State, westcentral region of Brazil. Fieldwork acquisitions were carried out in February/2001 and August/2002 in order to understand avulsion processes that are occurring within the Taquari alluvial megafan. The main subjects were to map channel, crevasses and floodplain morphology, as well as active sedimentary bedforms. Many GPR profiles were surveyed in the medium and lower Taquari River course. Subaqueous megaripples and exposed sand bars inside the Taquari channel were identified in the medium fan area. Similar features were observed in the lower fan channels, where there are also many crevasses in the marginal levees. During the flooding seasons the flow splays out in the floodplain where new distributary channels are being formed. As shown by GPR data, in the lower fan the Taquari channel is topographically higher than the adjacent floodplain, situation in which avulsion is a natural process of river course shifting. The lack of information about river morphology and dynamics is a major strain to better understand the sediment transport and the avulsion processes in the Taquari megafan. In this context, the GPR data obtained in wet and dry seasons, integrated to sedimentological information, have been very important to characterize the fluvial dynamics and the avulsion phenomena.

Application of GPR in the study of a modern alluvial megafan: The case of the Taquari river in Pantanal wetland, West-Central Brazil

A novel and timely ground penetrating radar (GPR) survey has been carried out in Pantanal from Mato Grosso State, west-central Brazil. Fieldwork was carried out on February/2001 and August/2002 in an attempt to understand avulsion processes that are occurring within Taquari alluvial megafan. The main objectives were to map channel, crevasse and floodplain morphology, as well as active sedimentary bedforms. Many GPR profiles were acquired in the medium and lower Taquari River course. Subaqueous megaripples and exposed sand bars were identified in the medium fan area. Similar features were observed in the lower fan channels, where there were many crevasses in the marginal levees. During the flooding seasons, the flow splays out on to the floodplain, where new distributary channels are being formed. GPR data show that the lower fan, Taquari channel is topographically higher than the adjacent floodplain, thus favoring avulsion as a natural process of river course shifting. GPR data obtained during the wet and dry seasons, together with sedimentology information have been very important in characterizing the fluvial dynamics, and avulsion phenomena. © 2005 Springer Science+Business Media, Inc.

Distributions and phylogeographic data of rheophilic freshwater fishes provide evidences on the geographic extension of a central-brazilian amazonian palaeoplateau in the area of the present day Pantanal Wetland

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Channel arrangements and depositional styles in the Sao Lourenco fluvial megafan, Brazilian Pantanal wetland

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Phylogenetic and host-parasite relationship analysis of Henneguya multiplasmodialis n. sp infecting Pseudoplatystoma spp. in Brazilian Pantanal wetland

A new species of the genus Henneguya (Henneguya multiplasmodialis n. sp.) was found infecting the gills of three of 89 specimens (3.3%) of Pseudoplatystoma corruscans and two of 79 specimens (2.6%) of Pseudoplatystoma reticulatum from rivers in the Pantanal wetland, Brazil. Partial sequencing of the 18S rDNA gene of the spores obtained from one plasmodium from the gills of P. corruscans and other one from the gills of P. reticulatum, respectively, resulted in a total of 1560 and 1147 base pairs. As the spores of H. multiplasmodialis n. sp. resemble those of Henneguya corruscans, which is also a parasite of P. corruscans, sequencing of the 18S rDNA gene of the spores of H. corruscans found on P. corruscans caught in the Brazilian Pantanal wetland was also provided to avoid any taxonomic pendency between these two species, resulting in 1913 base pairs. The sequences of H. multiplasmodialis n. sp. parasite of P. corruscans and P. reticulatum and H. corruscans did not match any of the Myxozoa available in the GenBank. The similarity of H. multiplasmodialis n. sp. obtained from P. corruscans to that from P. reticulatum was of 99.7%. Phylogeny revealed a strong tendency among Henneguya species to form clades based on the order and/or family of the host fish. H. multiplasmodialis n. sp. clustered in a clade with Henneguya eirasi and H. corruscans, which are also parasites of siluriforms of the family Pimelodidae and, together with the clade composed of Henneguya spp. parasites of siluriforms of the family Ictaluridae, formed a monophyletic clade of parasites of siluriform hosts. The histological study revealed that the wall of the plasmodia of H. multiplasmodialis n. sp. were covered with a stratified epithelium rich in club cells and supported by a layer of connective tissue. The interior of the plasmodia had a network of septa that divided the plasmodia into numerous compartments. The septa were composed of connective tissue also covered on both sides with a stratified epithelium rich in club cells. Inflammatory infiltrate was found in the tissue surrounding the plasmodia as well as in the septa. (C) 2011 Elsevier B.V. All rights reserved.

Hydrochemical variability at the Upper Paraguay Basin and Pantanal wetland

Compartmentalization is a prerequisite to understand large wetlands that receive water from several sources. However, it faces the heterogeneity in space and time, resulting from physical, chemical and biological processes that are specific to wetlands. The Pantanal is a vast seasonally flooded continental wetland located in the centre of South America. The chemical composition of the waters that supply the Pantanal (70 rivers) has been studied in order to establish a compartmentalization of the wetland based on soil-water interactions. A PCA-based EMMA (End-Members Mixing Analysis) procedure shows that the chemistry of the rivers can be viewed as a mixture of 3 end-members, influenced by lithology and land use, and delimiting large regions. Although the chemical composition of the end-members changed between dry and wet seasons, their spatial distribution was maintained. The results were extended to the floodplain by simple tributary mixing calculation according to the hydrographical network and to the areas of influence for each river when in overflow conditions. The resulting map highlights areas of high geochemical contrast on either side of the river Cuiaba in the north, and of the rivers Aquidauana and Abobral in the south. The PCA-based treatment on a sampling conducted in the Nhecolandia, a large sub region of the Pantanal, allowed the identification and ordering of the processes that control the geochemical variability of the surface waters. Despite an enormous variability in electrical conductivity and pH, all data collected were in agreement with an evaporation process of the Taquari River water, which supplies the region. Evaporation and associated saline precipitations (Mg-calcite, Mg-silicates K-silicates) explained more than 77% of the total variability in the chemistry of the regional surface water sampling.

First record of Trypanosoma sp. (Protozoa: Kinetoplastida) in tuvira (Gymnotus aff. inaequilabiatus) in the Pantanal wetland, Mato Grosso do Sul State, Brazil

O objetivo do presente estudo foi reportar a infecção por Trypanosoma sp. em tuviras (Gymnotus aff. inaequilabiatus) oriundas do Pantanal Sul-mato-grossense, Brasil. Dez peixes provenientes do rio Paraguai, Pantanal Sul-mato-grossense, foram avaliados quanto à presença de hemoflagelados. Tripomastigotas de Trypanosoma sp. foram observados nas extensões sanguíneas de três peixes (30% de prevalência), e algumas formas encontravam-se em divisão. Por meio do exame a fresco e da centrifugação do sangue em capilar de hematócrito como métodos para diagnóstico, a taxa de prevalência foi de 80%. Esse é o primeiro relato de Trypanosoma sp. em tuviras no Brasil.

Root behavior of savanna species in Brazil's Pantanal wetland.

The objective of this study was to determine the maximum depth, structure, diameter and biomass of the roots of common woody species in two savanna physiognomies (savanna woodland and open woody savanna) in Brazil's Pantanal wetland. The root systems of 37 trees and 34 shrubs of 15 savanna species were excavated to measure their length and depth and estimate the total root biomass through allometric relationships with stem diameter at ground level. In general, statistical regression models between root weight and stem diameter at ground level showed a significance of P < 0.05 and R2 values close to or above 0.8. The average depths of the root system in wetland savanna woodland and open woody savanna are 0.8 ± 0.3 m and 0.7 ± 0.2 m, respectively, and differ from the root systems of savanna woody species in non-flooding areas, whose depth usually ranges from 3 to 19 m.Weattribute this difference to the adaptation of woody plant to the shallow water table, particularly during the wet season. This singularity of woody species in wetland savannas is important when considering biomass and carbon stocks for national and global carbon inventories.