Destino del hongo Entomopatógeno Paecilomyces fumosoroseus (Wize &Brawn) (Deuteromycotina: Hyphomycetes) en suelos agrícolas

Tesis (Maestría en Ciencias con Especialidad en Microbiología) UANL

Producción en diferentes medios de cultivo y sobrevivencia de blastosporas de Paecilomyces fumosoroseus (Wize) Brown & Smith (Deuteromycotina: Hyphomycetes)

Tesis (Doctorado en Ciencias con Especialidad en Biotecnología) UANL

Producción de esporas de Paecilomyces fumosoroseus en diferentes medios de cultivo líquidos

Tesis (Maestría en Ciencias con Especialidad en Microbiología) UANL

Efecto de las condiciones operacionales de fermentación sobre la producción de Paecilomyces Fumosoroseus cepa 612 en cultivo sumergido

Tesis (Doctorado en Ciencias con Especialidad en Biotecnología) UANL

Secado y formulación de blastoesporas de Paecilomyces fumosoroseus(Hyphomycetes) producidos en dos medios líquidos diferentes

Tesis (Doctorado en Ciencias con Especialidad en Biotecnología) UANL

Cepa mutante de Paecilomyces fumosoroseus sobreproductora de quitinasas con mayor virulencia contra mosquita blanca (Bemisia tabaci) y falso mediador de la col (Trichoplusia ni)

Tesis (Doctor en Ciencias con Especialidad en Biotecnología) UANL

Producción de Paecilomyces fumosoroseus Brown & Smith y evaluación de su efectividad biólogica contra Bemisia argentifolii Bellows & Perring en cultivo de algodón Gossypium hirsutum L.

Tesis (Doctorado en Ciencias con especialidad en Biotecnología) U.A.N.L., Facultad de Ciencias Biológicas, 2007.

Efectos de micotoxicidad de fungicidas sobre formulados de los entomopatogenos Paecilomyces fumosoroseus y Beauveria bassiana utilizados contra la mosquita blanca (Bemisia argentifolii).

Tesis (Doctor en Ciencias con Especialidad en Biotecnología) UANL, 2011.

Atividade patogênica dos fungos Metarhizium anisopliae, Beauveria bassiana e Paecilomyces fumosoroseus para Chrysomya puloria (Wiedemann, 1830) (Diptera: Calliphoridae)

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

The effect of host plants on Tetranychus evansi, Tetranychus urticae (Acari: Tetranychidae) and on their fungal pathogen Neozygites floridana (Entomophthorales: Neozygitaceae)

In a series of tritrophic-level interaction experiments, the effect of selected host plants of the spider mites, Tetranychus evansi and Tetranychus urticae, on Neozygites floridana was studied by evaluating the attachment of capilliconidia, presence of hyphal bodies in the infected mites, mortality from fungal infection, mummification and sporulation from fungus-killed mite cadavers. Host plants tested for T. evansi were tomato, cherry tomato, eggplant, nightshade, and pepper while host plants tested for T. urticae were strawberry, jack bean, cotton and Gerbera. Oviposition rate of the mites on each plant was determined to infer host plant suitability while host-switching determined antibiosis effect on fungal activity. T. evansi had a high oviposition on eggplant, tomato and nightshade but not on cherry tomato and pepper. T. urticae on jack bean resulted in a higher oviposition than on strawberry, cotton and Gerbera. Attachment of capilliconidia to the T. evansi body, presence of hyphal bodies in infected T. evansi and mortality from fungal infection were significantly higher on pepper, nightshade and tomato. The highest level of T. evansi mummification was observed on tomato. T. evansi cadavers from tomato and eggplant produced more primary conidia than those from cherry tomato, nightshade and pepper. Switching N. floridana infected T. evansi from one of five Solanaceous host plants to tomato had no prominent effect on N. floridana performance. For T. urticae, strawberry and jack bean provided the best N. floridana performance when considering all measured parameters. Strawberry also had the highest primary conidia production. This study shows that performance of N. floridana can vary with host plants and may be an important factor for the development of N. floridana epizootics. (C) 2011 Elsevier Inc. All rights reserved.

Visible light during mycelial growth and conidiation of Metarhizium robertsii produces conidia with increased stress tolerance

Light conditions during mycelial growth are known to influence fungi in many ways. The effect of visible-light exposure during mycelial growth was investigated on conidial tolerance to UVB irradiation and wet heat of Metarhizium robertsii, an insect-pathogenic fungus. Two nutrient media and two light regimens were compared. Conidia were produced on (A) potato dextrose agar plus yeast extract medium (PDAY) (A1) under dark conditions or (A2) under continuous visible light (provided by two fluorescent lamps with intensity 5.4 W m-2). For comparison, the fungus was also produced on (B) minimal medium (MM) under continuous-dark incubation, which is known to produce conidia with increased tolerance to heat and UVB radiation. The UVB tolerances of conidia produced on PDAY under continuous visible light were twofold higher than conidia produced on PDAY medium under dark conditions, and this elevated UVB tolerance was similar to that of conidia produced on MM in the dark. The heat tolerance of conidia produced under continuous light was, however, similar to that of conidia produced on MM or PDAY in the dark. Conidial yield on PDAY medium was equivalent when the fungus was grown either under continuous-dark or under continuous-light conditions.

Efficiency of entomopathogenic fungi in the control of eggs and larvae of the horn fly Haematobia irritans (Diptera: Muscidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Entomopathogenic fungal activity against pupae and adult Haematobia irritans (Diptera: Muscidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influence of culture media in viability test of conidia of entomopathogenic fungi

O presente trabalho objetivou investigar se meios de cultura utilizados em teste de viabilidade afetam a germinação de conídios de cinco isolados de Lecanicillium lecanii, cinco de Beauveria bassiana e quatro de Paecilomyces fumosoroseus. Os testes foram realizados em lâminas de microscopia contendo um dos seguintes meios de cultura: Ágar-água (AA), Meio Mínimo (MM), Batata, dextrose e ágar (BDA), Batata, dextrose, ágar e 1% de extrato de levedura (BDAL), Sabouraud, dextrose, ágar e extrato de levedura (SDAL) e Meio Completo (MC). Delimitaram-se três áreas por lâmina e em cada uma aplicou-se 0,05mL de uma suspensão com concentração de 5,5 x 105 conídios ml-1. Para cada isolado foi realizado um bioensaio, com seis tratamentos e cinco repetições. Avaliou-se a germinação 15 horas após incubação, a 26±0,5ºC. Os meios de cultura influíram na capacidade de germinação das três espécies estudadas, ocorrendo variações inter e intraespecíficas. Verificou-se que os meios Completo e BDA proporcionaram as maiores porcentagens de germinação dos isolados de L. lecanii, sendo que e as menores foram obtidas nos meios SDAL e AA. Os meios ricos em nutrientes (BDA, BDAL e Completo) favoreceram a germinação dos isolados de B. bassiana, o que não ocorreu com os meios pobres (AA e MM). Nos meios Completo e BDA foram obtidas as maiores porcentagens de germinação dos isolados de P. fumosoroseus. As menores percentagens, por sua vez, foram obtidas no meio SDAL. Entretanto, alguns isolados apresentaram alta germinação em meios pobres em nutrientes (AA e MM).

Fungos entomopatogênicos para o controle da mosca-dos- chifres Haematobia irritans em laboratório e campo

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)