La formación básica con personas adultas gitanas 'Nebó Dikhel' (nuevas miradas).

Se consta de una serie de unidades didácticas elaboradas a partir de una amplia y dilatada experiencia docente con población gitana adulta. Cada unidad didáctica cuenta con una sección técnica y un material didáctico listo para usarse en el aula. En la sección técnica, que puedes encontrar, en formato PDF, en la Carpeta del profesorado del menú principal, se han incluido objetivos, contenidos, metodología, evaluación y toda aquella información que puede ser útil para realizar una buena programación con estas unidades. Por otra parte, el material didáctico incluido en la Carpeta del alumnado puede ser imprimido y usado directamente en el aula o bien, dada la naturaleza interactiva del mismo en algunos casos, puede usarse directamente trabajando en un ordenador. Asimismo, se presenta un apartado de carácter histórico y cultural referido al pueblo gitano; por último, también se ofrece un documento de carácter educativo y teórico. Con estos dos documentos se intenta mostrar y aclarar el contexto que probablemente se presente en posibles acciones educativas con población gitana adulta.

Nebī'īm rīšōnīm

nach dem masoretischen Texte ... übers., m. Anm. von J[osef] Johlson

Rada wsselikých zwiřat nerozumných neb zhowadilých, y ptactwa, kterau člowěku wsselikého powoláni dáwagi, w ćemby gegich přirozeni následowati, a gakých powah k zpráwě žiwota dobrého se wystřihati měl. Od Giřiho Melantrycha z Awentýnu. Kniha vžitečná y kratochwilná.

"Předmluva" signed J.D. [i.e. Josef Dobrovský]

Kniha užitečná y kratochwilná, genž slowe: Rada, wsselikých zwhjřat nerozumných neb zhowadilých, y ptactwa ... Od Giřjho Melantrycha z Awentýnu.

[Published by the Komise pro vydávání spisů Josefa Dobrovského in 1939.] Inserted is a pamphlet with modernized title of the 4th ed. and critical material.

Excellent Lincoln Highway Markers west of Eureka, Neb.

Photo album title: Detroit to San Francisco over Lincoln Highway. May 27-June 18, 1915. Henry B. Joy; A.F. Bement; E. Eisenhut

A Lagrangian Identification of the Main Sources of Moisture Affecting Northeastern Brazil during Its Pre-Rainy and Rainy Seasons

This work examines the sources of moisture affecting the semi-arid Brazilian Northeast (NEB) during its pre-rainy and rainy season (JFMAM) through a Lagrangian diagnosis method. The FLEXPART model identifies the humidity contributions to the moisture budget over a region through the continuous computation of changes in the specific humidity along back or forward trajectories up to 10 days period. The numerical experiments were done for the period that spans between 2000 and 2004 and results were aggregated on a monthly basis. Results show that besides a minor local recycling component, the vast majority of moisture reaching NEB area is originated in the south Atlantic basin and that the nearby wet Amazon basin bears almost no impact. Moreover, although the maximum precipitation in the ""Poligono das Secas'' region (PS) occurs in March and the maximum precipitation associated with air parcels emanating from the South Atlantic towards PS is observed along January to March, the highest moisture contribution from this oceanic region occurs slightly later (April). A dynamical analysis suggests that the maximum precipitation observed in the PS sector does not coincide with the maximum moisture supply probably due to the combined effect of the Walker and Hadley cells in inhibiting the rising motions over the region in the months following April.

Expressão e purificação da proteína E3 do vírus chikungunya (CHIKV)

RESUMO: O vírus chikungunya (CHIKV) é um vírus de RNA, com invólucro, da família Togaviridae, transmitido por mosquitos Aedes spp. Distribuído por largas regiões de África e Ásia, causa grandes epidemias de artrite grave. A semelhança de sintomas com outras doenças como a dengue e a malária e a persistência de IgM específicas, dificultam o diagnóstico da infeção por CHIKV. A deteção no sangue de E3, uma glicoproteína viral secretada, a incluir num ensaio imunoenzimático poderá melhorar o diagnóstico nos países onde as técnicas de biologia molecular são de difícil acesso. Para testar a utilidade de E3 num ensaio de diagnóstico, esta deverá ser expressa em quantidade, purificada e usada para produção de anticorpos específicos. Para expressar E3 numa forma solúvel, suscetível de ser purificada num único passo cromatográfico sem proteases, recorreu-se à estratégia da fusão com o domínio de ligação à quitina (CBD)-inteína (IMPACT™ System, NEB). A sequência codificadora de E3 foi amplificada a partir de RNA viral, clonada em pTYB21 e expressa em E. coli como uma proteína de fusão insolúvel de 64 kDa. A expressão a 12ºC induzida por IPTG 0,1 mM aumentou a solubilidade de CBD-inteína-E3. A aplicação de lisados celulares em colunas de quitina originou a retenção de CBD-inteína-E3 na matriz. Porém, a autoclivagem da inteína na coluna, induzida com reagentes tiol, foi pouco eficiente e mesmo a proteína E3 separada não eluiu da coluna. E3 foi ainda expressa em E. coli com uma cauda de seis histidinas (E3[His]6) por clonagem no vetor pET28b(+). Lisados celulares aplicados em colunas de níquel permitiram a eluição de uma proteína de 9 kDa, compatível com a massa molecular estimada para E3[His]6, ainda que com outros contaminantes proteicos. A identidade da proteína de 9 kDa será confirmada pela indução de anticorpos com esta preparação e reatividade daqueles com células infetadas com CHIKV.----------------ABSTRACT: Chikungunya virus (CHIKV) is an enveloped, positive strand RNA virus belonging to the family Togaviridae. Transmitted by Aedes spp mosquitoes, CHIKV causes large epidemics of severe arthritogenic disease in Africa and Asia and represents a serious threat in countries where vectors are present. Symptoms similarity with other diseases, e.g. dengue and malaria, along with CHIKV IgM persistence turns accurate CHIKV diagnosis a difficult task in low-income countries. Detection of E3, a small secreted viral glycoprotein, to be included in an immunoenzymatic test was envisaged as a possible improvement in CHIKV diagnosis. To test the diagnostic value of E3, recombinant E3 should be expressed and purified to generate antibodies. In order to express CHIKV E3 in a soluble form amenable to purification by a single step affinity chromatography, the chitin binding domain (CBD)-intein fusion strategy without proteases (IMPACT™ System, NEB) was employed. The E3 coding sequence was amplified from viral RNA, cloned in pTYB21 and expressed in E. coli ER2566 as an insoluble 64 kDa CBD-intein-E3 fusion protein. Solubility was partially achieved by lowering the expression temperature to 12ºC and the inducer (IPTG) concentration to 0.1 mM. Clarified cell lysate loaded onto a chitin column allowed ligation of the fusion protein but the intein-mediated cleavage efficiency was low and E3 failed to elute from the column as demonstrated by SDS-PAGE. E3 was further expressed with a six histidine tag, E3[His]6, employing the pET System (Novagen). E3[His]6 was expressed in E. coli Rosetta (30ºC, 0.4 mM IPTG) as a 9 kDa protein. Soluble cell extracts in 20-40 mM imidazole, applied onto a nickel column and eluted with 500 mM imidazole yielded a protein preparation enriched in the 9kDa protein. The 9 kDa will be used as antigen to generate antibodies that upon reaction with CHIKV infected cells will confirm its identity.

Nematódeos e bactérias em fôlhas de alcachôfra

Diseased plants of artichoke (Cynara scolimus L.) from the Instituto Agronômico, Campinas, Brazil, harboured nematodes in the leaves. The nematodes proved to belong to the species Protorhabditis oxyuris (Claus, 1862) Dougherty, 1955. As it could not be charged as the primary agent of the disease, a study of the bacteria found in the material was made. This disclosed that 4 different bacterial forms were present, namely Bacillus subtilis, Bacterium udum, Xanthomonas beticola var. cynarae n. var., and Cory neb acterium fascians var. cynarae n. var. The typical forms of X. beticola and of C. fascians are known as pathogenic to a number of plants: but, in the present case, unfortunately no inoculation trial could be made as an attempt to clear up the possible pathogenicity of any of them also to Cynara scolimus. The nematode P. oxyuris is the secondary agent, which however possibly play an important part in the distribution of the bacteria, as it has been repeatedly pointed out by previous writers with reference to closely related species. The symptoms of the disease are described. The main injuries found on leaves were necrotic areas progressing from the distal part of the limb, resulting in a great reduction of the leaf superficies.

Oligocene sedimentation, stratigraphy, paleoecology and paleoclimatology in the big Badlands of South Dakota [by] John Clark, James R. Beerbower [and] Kenneth K. Kietzke.

v.5(1967)

TRAIP is a regulator of the spindle assembly checkpoint.

Accurate chromosome segregation during mitosis is temporally and spatially coordinated by fidelity-monitoring checkpoint systems. Deficiencies in these checkpoint systems can lead to chromosome segregation errors and aneuploidy, and promote tumorigenesis. Here, we report that the TRAF-interacting protein (TRAIP), a ubiquitously expressed nucleolar E3 ubiquitin ligase important for cellular proliferation, is localized close to mitotic chromosomes. Its knockdown in HeLa cells by RNA interference (RNAi) decreased the time of early mitosis progression from nuclear envelope breakdown (NEB) to anaphase onset and increased the percentages of chromosome alignment defects in metaphase and lagging chromosomes in anaphase compared with those of control cells. The decrease in progression time was corrected by the expression of wild-type but not a ubiquitin-ligase-deficient form of TRAIP. TRAIP-depleted cells bypassed taxol-induced mitotic arrest and displayed significantly reduced kinetochore levels of MAD2 (also known as MAD2L1) but not of other spindle checkpoint proteins in the presence of nocodazole. These results imply that TRAIP regulates the spindle assembly checkpoint, MAD2 abundance at kinetochores and the accurate cellular distribution of chromosomes. The TRAIP ubiquitin ligase activity is functionally required for the spindle assembly checkpoint control.

Seuraajasuunnitteluprosessi ja sen toimivuus KONE Oyj:n New Equipment Business -liiketoimintayksikössä

Tämä tutkimus käsittelee seuraajasuunnitteluprosessia ja sen toimivuutta. Tutkimus on tapaustutkimus, jossa tutkittava ilmiö eli seuraajasuunnittelu ja kohteena oleva tapaus eli organisaatio KONE Oyj:n yksi liiketoimintayksikkö New Equipment Business, NEB voidaan erottaa toisistaan. Tutkimuksen kohteena on seuraajasuunnitteluprosessin toimivuus tapausorganisaatiossa. Tutkimuksen tarkoituksena on hankkia tietoa ja ymmärtää prosessin toteutumista sekä löytää jo käytössä olevat parhaimmat käytännöt sekä merkittävimmät kehitysalueet. Tutkimuksen lähestymistapa on kvalitatiivinen. Seuraajasuunnitteluprosessin toimivuutta tutkittiin tekemällä teemahaastatteluja tutkimuksen kohteena olevan organisaation eri funktioiden henkilöstöpäälliköiden ja yksiköiden johtajien kanssa. Haastattelujen lisäksi tutkimuksessa käytettiin tausta-aineistona yrityksen sisäisiä arkistointitietoja ja materiaalia. Työn tuloksena saatiin selville, miten seuraajasuunnitteluprosessi toteutuu kohdeorganisaatiossa. Tutkimuksen tulosten perusteella näyttää siltä, että panostamalla talenttien kehitystoimenpiteisiin sekä lisäämällä henkilöiden liikkuvuutta yli organisaatiorajojen voidaan kehittää seuraajasuunnitteluprosessia vastaamaan tulevaisuuden haasteisiin.

Estudo estatístico sobre eventos de precipitação intensa no nordeste do Brasil

The Northeast of Brazil (NEB) shows high climate variability, ranging from semiarid regions to a rainy regions. According to the latest report of the Intergovernmental Panel on Climate Change, the NEB is highly susceptible to climate change, and also heavy rainfall events (HRE). However, few climatology studies about these episodes were performed, thus the objective main research is to compute the climatology and trend of the episodes number and the daily rainfall rate associated with HRE in the NEB and its climatologically homogeneous sub regions; relate them to the weak rainfall events and normal rainfall events. The daily rainfall data of the hydrometeorological network managed by the Agência Nacional de Águas, from 1972 to 2002. For selection of rainfall events used the technique of quantiles and the trend was identified using the Mann-Kendall test. The sub regions were obtained by cluster analysis, using as similarity measure the Euclidean distance and Ward agglomerative hierarchical method. The results show that the seasonality of the NEB is being intensified, i.e., the dry season is becoming drier and wet season getting wet. The El Niño and La Niña influence more on the amount of events regarding the intensity, but the sub-regions this influence is less noticeable. Using daily data reanalysis ERAInterim fields of anomalies of the composites of meteorological variables were calculated for the coast of the NEB, to characterize the synoptic environment. The Upper-level cyclonic vortex and the South atlantic convergene zone were identified as the main weather systems responsible for training of EPI on the coastland

Modelagem estocástica do índice de radiação ultravioleta na costa leste do nordeste do Brasil

The increase in ultraviolet radiation (UV) at surface, the high incidence of non-melanoma skin cancer (NMSC) in coast of Northeast of Brazil (NEB) and reduction of total ozone were the motivation for the present study. The overall objective was to identify and understand the variability of UV or Index Ultraviolet Radiation (UV Index) in the capitals of the east coast of the NEB and adjust stochastic models to time series of UV index aiming make predictions (interpolations) and forecasts / projections (extrapolations) followed by trend analysis. The methodology consisted of applying multivariate analysis (principal component analysis and cluster analysis), Predictive Mean Matching method for filling gaps in the data, autoregressive distributed lag (ADL) and Mann-Kendal. The modeling via the ADL consisted of parameter estimation, diagnostics, residuals analysis and evaluation of the quality of the predictions and forecasts via mean squared error and Pearson correlation coefficient. The research results indicated that the annual variability of UV in the capital of Rio Grande do Norte (Natal) has a feature in the months of September and October that consisting of a stabilization / reduction of UV index because of the greater annual concentration total ozone. The increased amount of aerosol during this period contributes in lesser intensity for this event. The increased amount of aerosol during this period contributes in lesser intensity for this event. The application of cluster analysis on the east coast of the NEB showed that this event also occurs in the capitals of Paraiba (João Pessoa) and Pernambuco (Recife). Extreme events of UV in NEB were analyzed from the city of Natal and were associated with absence of cloud cover and levels below the annual average of total ozone and did not occurring in the entire region because of the uneven spatial distribution of these variables. The ADL (4, 1) model, adjusted with data of the UV index and total ozone to period 2001-2012 made a the projection / extrapolation for the next 30 years (2013-2043) indicating in end of that period an increase to the UV index of one unit (approximately), case total ozone maintain the downward trend observed in study period