Morphological alterations in leave of micropropagated pineapple plants cv. IAC Gomo-de-mel acclimatizated in different conditions of luminosity

(Morphological alterations in leave of micropropagated pineapple plants cv. IAC Gomo-de-mel acclimatizated in different conditions of luminosity). Microprapagated plants usually show difficulties to adapt to ex vitro conditions, and many times are submitted to the rustication process to aim the reduction of all the impacts resulting from the environmental changes. Once the leaf and its annexes are important indicators of adaptability strategies of the plants to adverse environmental conditions, the objective of this work was to compare the leaf anatomy of pineapple cv. IAC Gomo-de-mel in vitro cultivated plants with microplants acclimatized in different conditions of luminosity, under mesh, with 50% of shading and directly exposed to sunlight, to verify the needed of rustication process on this cultivar. Evaluations of the leaf epidermis using light and electronic scanning microscopy showed an increase on scale density in both leaves surfaces of the ex vitro microplants, mainly related to the ones directly exposed to sunlight. Subsequent observations showed an increase on cuticle thickness, on wavy contours of epidermal cells, and on the distribution and quantity of mesophyll fibers, evidencing the light conditions interference in morphological characteristics of these microplants. These alterations had not harmed microplant development, showing that are not need of rustication stages on the acclimatization process of this cultivar.

Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion

Twenty endophytic bacteria were isolated from the meristematic tissues of three varieties of strawberry cultivated in vitro, and further identified, by FAME profile, into the genera Bacillus and Sphingopyxis. The strains were also characterized according to indole acetic acid production, phosphate solubilization and potential for plant growth promotion. Results showed that 15 strains produced high levels of IAA and all 20 showed potential for solubilizing inorganic phosphate. Plant growth promotion evaluated under greenhouse conditions revealed the ability of the strains to enhance the root number, length and dry weight and also the leaf number, petiole length and dry weight of the aerial portion. Seven Bacillus spp. strains promoted root development and one strain of Sphingopyxis sp. promoted the development of plant shoots. The plant growth promotion showed to be correlated to IAA production and phosphate solubilization. The data also suggested that bacterial effects could potentially be harnessed to promote plant growth during seedling acclimatization in strawberry.

Growth, crown structure and seed production of birch seedlings, grafts and micropropagated plants

Summary

Effects of arbuscular mycorrhizal fungi and phosphorus fertilization on post vitro growth of micropropagated Zingiber officinale roscoe

The rhizomes of Zingiber officinale Roscoe (ginger) are widely used for their medicinal and flavoring properties, whereas the influence of root symbionts on their growth is poorly understood. In this study, the effects of phosphate fertilization and inoculation with a mixture of arbuscular mycorrhizal fungi (AMF) (isolates Glomus clarum RGS101A, Entrophospora colombiana SCT115A and Acaulospora koskei SPL102A) on survival, growth and development of micropropagated ginger were investigated. After transplanting to post vitro conditions, the ginger microplants were subjected to the following treatments: a) AMF mixture, b) P addition (25 mg kg-1), c) AMF + P, and d) non-mycorrhizal control without P addition. After eight months of growth, survival ranged from 86 to 100 % in the AMF and AMF+P treatments versus 71 % survival in control and P treatments. In the AMF, P and AMF+P treatments, the shoot, root and rhizome biomass production were significantly larger than in the control plants. In the non-mycorrhizal control plants the leaf number, leaf area, number of shoots/plants, and shoot length were significantly lower than in the AMF, P and AMF+P treatments. Root colonization ranged from 81 to 93 % and was not affected by P application. The data confirmed the response of several growth variables of micropropagated ginger to mycorrhizal colonization and P addition.

Agronomic characteristics of 'Prata-anã' and 'Maçã' bananas micropropagated

The experiment evaluated the agronomic characteristics of 'Prata-anã' and 'Maçã' banana plants, in the São Manuel region of São Paulo state. In the first crop cycle, the number of days from planting to inflorescence and from inflorescence to harvest; the number of shoots until the appearance of inflorescence and during the harvest period; active leaves number at inflorescence appearance and during the harvest period. Were evaluated yield parameters: like average weight of bunch, hand, rachis and fruit; average fruit number per hand and bunch; average fruit diameter and length. Incidences of plague diseases, as well as their severity were also evaluated. Delineation was totally at random, with 2 treatments, 15 replications and 5 useful plants per experimental plot. Both cultivars were characterized by values of descriptive average statistics and standard deviation, for characteristic interests. Number of days from planting to harvest was similar for both 'Prata-anã' and 'Maçã', 574 and 567 days respectively. Banana plants showed good phytosanitary quality throughout the whole cycle.

Acclimatization and leaf anatomy of micropropagated fig plantlets

The survival of micropropagated plants during and after acclimatization is a limiting process to plant establishment. There is little information on how the anatomy of vegetative organs of Ficus carica can be affected by culture conditions and acclimatization. The present research aimed to study the effects of time on culture medium and substrates during the acclimatization of fig tree plantlets produced in vitro, characterizing some leaf anatomy aspects of plantlets cultured in vitro and of fig trees produced in field. Plantlets previously multiplied in vitro were separated and transferred into Wood Plant Medium (WPM) where they were kept for 0, 15, 30, 45 and 60 days. Different substrates were tested and studies on leaf anatomy were performed in order to compare among plantlets grown in vitro, plantlets under 20, 40 and 60 days of acclimatization, and field grown plants. Keeping plantlets for 30 days in WPM allowed better development in Plantmax during acclimatization. Field grown plants presented higher number of stomata, greater epicuticular wax thickness and greater leaf tissue production compared to in vitro ones. The leaf tissues of in vitro plantlets show little differentiation and have great stomata number compared with acclimatized plants, which reduce the number of stomata during the acclimatization process.

Agronomic characteristics of 'Prata-anã' and 'Maçã' bananas micropropagated

O experimento foi instalado com o objetivo de estudar o desempenho agronômico de cultivares de bananeira Prata-anã e Maçã, na região de São Manuel-SP. Foram avaliadas as seguintes características de desenvolvimento, no primeiro ciclo de produção: número de dias do plantio à floração e da mesma à colheita, número de rebentos (filhos) emitidos até o surgimento da inflorescência e na época da colheita, número de folhas ativas na época do aparecimento da inflorescência e da colheita, e características de rendimento, como peso médio do cacho, ráquis, penca e fruto, número médio de frutos por penca e cacho, comprimento e diâmetro médio dos frutos. Também foi avaliada a incidência de pragas e doenças. Foi empregado o delineamento inteiramente casualizado, com 2 tratamentos, 15 repetições e 5 plantas úteis por parcela experimental. As duas cultivares foram caracterizadas pelos valores das estatísticas descritivas, média e desvio-padrão, para as características de interesse no experimento. Foram estabelecidas possíveis correlações entre medidas de desenvolvimento e produção. As duas cultivares apresentaram ciclo do plantio até a colheita de 574 dias para 'Prata-anã' e 567 dias para 'Maçã', sendo que o ciclo produtivo foi maior na 'Prata-anã'. Não houve problemas de pragas e doenças em todo o ciclo de produção, nas duas cultivares avaliadas.

A simplified PCR test for early detection of dwarf off-types in micropropagated Cavendish bananas (Musa spp. AAA)

The dwarf somaclonal variant is a major problem affecting micropropagation of the banana cultivar Williams (Musa spp. AAA; subgroup Cavendish). This problem arises from genetic changes that occur during the tissue culture process. Early identification of this problem is difficult and propagators must wait until plants are ex vitro in order to visualise the dwarfism phenotype. In this study, we have improved a SCAR-based molecular diagnostic technique, developed by Damasco et al. [Acta Hortic. 461 (1997) 157], for the early identification of dwarf off-types. We have included a positive internal control in a multiplex PCR and adapted the technique for use with small amounts of fresh in vitro leaf material as PCR template. The control product is a 500 bp fragment from 18S rRNA and is amplified in all tissues irrespective of phenotype. The use of small in vitro leaf material removing the need for genomic DNA extraction. (C) 2004 Elsevier B.V. All rights reserved.

Isolation of micropropagated strawberry endophytic bacteria and assessment of their potential for plant growth promotion.

Twenty endophytic bacteria were isolated from the meristematic tissues of three varieties of strawberry cultivated in vitro, and further identified, by FAME profile, into the genera Bacillus and Sphingopyxis. The strains were also characterized according to indole acetic acid production, phosphate solubilization and potential for plant growth promotion. Results showed that 15 strains produced high levels of IAA and all 20 showed potential for solubilizing inorganic phosphate. Plant growth promotion evaluated under greenhouse conditions revealed the ability of the strains to enhance the root number, length and dry weight and also the leaf number, petiole length and dry weight of the aerial portion. Seven Bacillus spp. strains promoted root development and one strain of Sphingopyxis sp. promoted the development of plant shoots. The plant growth promotion showed to be correlated to IAA production and phosphate solubilization. The data also suggested that bacterial effects could potentially be harnessed to promote plant growth during seedling acclimatization in strawberry

Identification of endophytic bactéria from micropropagated yacon (Smallanthus sonchifolius) crowing under autotrophic and Heterotrophic systems.

Yacon, Smallanthus sonchifolius, an Andean species. is a rich source of dictetíc oligofructans with low glucose content. proteins and phenolic compounds. These constituents have shown efficacy in the prevention of diet-related ehronic diseases, including gastroin-testinal disorders and diabetes |1,2|. Yacon is part of a research program at the National Center for Natural Products Research (NCNPR) and University of Mississippi Field Station to develop new alternative root crops for Mississippi while attempting to im-prove the diet of low incorne families. Yacon can be easily propa-gated by cultings. Virus and nematode infections have been re-ported on plants propagated by cuttings in Brazil. a country that hás adopted Yacon as specialty crop [3|. We have developed two culture systems. autotrophic and heterotrophic, to produce healthy plants. Herem we describe the presence of endophytic bactéria m micropropagated Yacon. In auxin free media, new roots were induced. Overa 15day period. the average root mduction per expiam was 5.45 to 8.75 under autotrophic and heterotrophic cul-tures, respectively. Root lenglh vaned between 3 and 60mrn. The presence of root hairs and lateral roots was noticed only in auto-trophic condilions. These beneficiai bactéria were identified and chemically ctiaracterized. Acknowledgement: This research work was partially supported by the USDA/ARS Cooperative Research Agreement No. 58-6408-2-009. Referentes; |1) Terada S. et ai. (2006] Yakugaku Zasshi 126(8): 665-669. (2| Valentová K. Ulri-chová j. (2003) Biomedical Papers 147: 119-130. [3| Mogor C. et ai, (2003) Acta Horticulturea 597: 311 -313.

Qualidade de luz na biometria e anatomia foliar de plântulas de Cattleya loddigesii L. (Orchidaceae) micropropagadas

A qualidade de luz pode alterar a morfogênese das plantas por meio de uma série de processos mediados por receptores de luz, principalmente na região do vermelho e azul. O objetivo do presente estudo foi verificar alterações anatômicas foliares e características biométricas de Cattleya loddigesii 'Tipo', cultivadas in vitro, sob diferentes malhas coloridas com nível de radiação de 50% de sombreamento. Plântulas oriundas de autopolinização e sementes germinadas in vitro, com aproximadamente 1,0cm de comprimento e com raízes, foram inoculadas em meio WPM e submetidas a diferentes condições de incubação. Testou-se o efeito de sombrites coloridos (vermelho e azul) sobre os frascos cultivados em casa de vegetação (CV) e sala de crescimento (SC), além dos tratamentos, nos dois ambientes, sem utilização das telas coloridas. A avaliação foi efetuada 180 dias após inoculação. Com os resultados obtidos, observou-se que o ambiente de cultivo promove alterações anatômicas e biométricas em plântulas de Cattleya loddigesii 'Tipo' micropropagadas. As alterações promovidas pelo cultivo em luz natural evidenciam maior capacidade fotossintética, por meio de maior diferenciação dos tecidos clorofilianos, promovendo uma superfície foliar anatomicamente adaptada à fase de aclimatização.

MODIFICATIONS ON LEAF ANATOMY OF BANANA DURING THE MICROPROPAGATION PROCESS

The study and understanding of alterations taking place during the micropropagation process can provide valuable information about this technology. The objective of this work was to evaluate the anatomical modifications in leaves of micropropagated banana (Musa spp.) plants during their adaptation to ex vitro conditions. Aseptic axillary shoots of `Preciosa` cultivar (AAAB) were rooted for 24 days in MS medium containing NAA (1mg.l(-1)) and agar (6g.l(-1)), and acclimatized for 120 days. The treatments consisted of leaves at different stages of development: T1 - leaves from plants at the end of in vitro rooting phase, T2 persistent leaves from plants after 30 days of acclimatization, T3 - new leaves from plants after 30 days of acclimatization (transition leaves). T4 - transition leaves from plants after 60 days, T5 - new leaves from plants after 60 days of acclimatization, and T6 - new leaves from plants after 120 days of acclimatization. A higher degree of differentiation and, thereby, better adaptation took place in leaves from leaf primordial differentiated in ex vitro conditions. The acclimatization phase is crucial for a greater thickness and differentiation of spongy and palisade parenchyma, and to correct the modifications of plants developed in vitro. The study of leaf anatomy provides a better understanding of alterations occurring in micropropagated banana plants.

Adubação foliar com macro e micronutrientes no crescimento de mudas micropropagadas do abacaxizeiro cv. Gold [Ananas comosus (L.) Merrill] em diferentes recipientes

Parte da Dissertação do primeiro autor, apresentada ao Centro de Ciências Agrárias da Universidade Federal do Espírito Santo/PPGPV, Alegre-ES, como parte das exigências para obtenção do grau de Mestre em Produção Vegetal/Fitotecnia.

Acclimatization of Tapeinochilos ananassae plantlets in association with arbuscular mycorrhizal fungi

The objective of this work was to assess the potential of three isolates of arbuscular mycorrhizal fungi to promote growth of micropropagated plantlets of Tapeinochilos ananassae during acclimatization. The experiment was carried out in greenhouse, in a completely randomized block design, with four inoculation treatments: non‑inoculated control and plants inoculated with Glomus etunicatum, Acaulospora longula or Gigaspora albida, with ten replicates. After 90 days, the following parameters were evaluated: survival rate, height, leaf and tiller number, leaf area, fresh and dry biomass, contents of macro‑ and micronutrients in the root and shoot, glomerospore number, and mycorrhizal colonization. The survival percentage was 100%, except for plants inoculated with G. albida (80%). The isolate G. etunicatum is more suitable for plant development, since it improves survival, growth, dry matter production, nutritional status, and vigor of T. ananassae micropropagated plants.