Impacts of traditional husbandry practices on exploitable levels of genetic diversity in cultured 'Tra' catfish (Pangasianodon hypophthalmus) in the Mekong Delta, Vietnam

Sutchi catfish (Pangasianodon hypophthalmus) – known more universally by the Vietnamese name ‘Tra’ is an economically important freshwater fish in the Mekong Delta in Vietnam that constitutes an important food resource. Artificial propagation technology for Tra catfish has only recently been developed along the main branches of the Mekong River where more than 60% of the local human population participate in fishing or aquaculture. Extensive support for catfish culture in general, and that of Tra (P. hypophthalmus) in particular, has been provided by the Vietnamese government to increase both the scale of production and to develop international export markets. In 2006, total Vietnamese catfish exports reached approximately 286,602 metric tons (MT) and were valued at 736.87 $M with a number of large new export destinations being developed. Total value of production from catfish culture has been predicted to increase to approximately USD 1 billion by 2020. While freshwater catfish culture in Vietnam has a promising future, concerns have been raised about long-term quality of fry and the effectiveness of current brood stock management practices, issues that have been largely neglected to date. In this study, four DNA markers (microsatellite loci: CB4, CB7, CB12 and CB13) that were developed specifically for Tra (P. hypophthalmus) in an earlier study were applied to examine the genetic quality of artificially propagated Tra fry in the Mekong Delta in Vietnam. The goals of the study were to assess: (i) how well available levels of genetic variation in Tra brood stock used for artificial propagation in the Mekong Delta of Vietnam (breeders from three private hatcheries and Research Institute of Aquaculture No2 (RIA2) founders) has been conserved; and (ii) whether or not genetic diversity had declined significantly over time in a stock improvement program for Tra catfish at RIA2. A secondary issue addressed was how genetic markers could best be used to assist industry development. DNA was extracted from fins of catfish collected from the two main branches of the Mekong River inf Vietnam, three private hatcheries and samples from the Tra improvement program at RIA2. Study outcomes: i) Genetic diversity estimates for Tra brood stock samples were similar to, and slightly higher than, wild reference samples. In addition, the relative contribution by breeders to fry in commercial private hatcheries strongly suggest that the true Ne is likely to be significantly less than the breeder numbers used; ii) in a stock improvement program for Tra catfish at RIA2, no significant differences were detected in gene frequencies among generations (FST=0.021, P=0.036>0.002 after Bonferroni correction); and only small differences were observed in alleles frequencies among sample populations. To date, genetic markers have not been applied in the Tra catfish industry, but in the current project they were used to evaluate the levels of genetic variation in the Tra catfish selective breeding program at RIA2 and to undertake genetic correlations between genetic marker and trait variation. While no associations were detected using only four loci, they analysis provided training in the practical applications of the use of molecular markers in aquaculture in general, and in Tra culture, in particular.

Insights into historical drainage evolution based on the phylogeography of the chevron snakehead fish (Channa striata) in the Mekong Basin

1. The phylogeography of freshwater taxa is often integrally linked with landscape changes such as drainage re-alignments that may present the only avenue for historical dispersal for these taxa. Classical models of gene flow do not account for landscape changes and so are of little use in predicting phylogeography in geologically young freshwater landscapes. When the history of drainage formation is unknown, phylogeographical predictions can be based on current freshwater landscape structure, proposed historical drainage geomorphology, or from phylogeographical patterns of co-distributed taxa. 2. This study describes the population structure of a sedentary freshwater fish, the chevron snakehead (Channa striata), across two river drainages on the Indochinese Peninsula. The phylogeographical pattern recovered for C. striata was tested against seven hypotheses based on contemporary landscape structure, proposed history and phylogeographical patterns of codistributed taxa. 3. Consistent with the species ecology, analysis of mitochondrial and microsatellite loci revealed very high differentiation among all sampled sites. A strong signature of historical population subdivision was also revealed within the contemporary Mekong River Basin (MRB). Of the seven phylogeographical hypotheses tested, patterns of co-distributed taxa proved to be the most adequate for describing the phylogeography of C. striata. 4. Results shed new light on SE Asian drainage evolution, indicating that the Middle MRB probably evolved via amalgamation of at least three historically independent drainage sections and in particular that the Mekong River section centred around the northern Khorat Plateau in NE Thailand was probably isolated from the greater Mekong for an extensive period of evolutionary time. In contrast, C. striata populations in the Lower MRB do not show a phylogeographical signature of evolution in historically isolated drainage lines, suggesting drainage amalgamation has been less important for river landscape formation in this region.

An assessment of the geographical scale of recurrent gene flow in wild populations of two species of Mekong River carps (Henicorhynchus spp.)

The Mekong is the most productive river fishery in the world, and such as, the Mekong River Basin (MRB) is very important to very large human populations across the region as a source of revenue (through fishing and marketing of aquatic resources products) and as the major source for local animal protein. Threats to biodiversity in the MRB, either to the fishery sector itself or to other sectors are a major concern, even though currently, fisheries across this region are still very productive. If not managed properly however, fish population declines will cause significant economic impact and affect livelihoods of local people and will have a major impact on food security and nutrition. Biodiversity declines will undoubtedly affect food security, income and socio-economic status of people in the MRB that depend on aquatic resources. This is an indicator of unsustainable development and hence should be avoided. Genetic diversity (biodiversity) that can be measured using techniques based on DNA markers; refers to variation within and among populations within the same species or reproductive units. In a population, new genetic variation is generated by sexual recombination contributed by individuals with mutations in genes and chromosomes. Over time, populations of a species that are not reproducing together will diverge as differential impacts of selection and genetic drift change their genetic attributes. For mud carp (Henicorhynchus spp.), understanding the status of breeding units in the MRB will be important for their long term persistence, sustainability and for implementing effective management strategies. Earlier analysis of stock structure in two economically important mud carp species (Henicorhynchus siamensis and H. lobatus) in the MRB completed with mtDNA markers identified a number of populations of both species where gene flow had apparently been interrupted or reduced but applying these data directly to management unit identification is potentially compromised because information was only available about female dispersal patterns. The current study aimed to address this problem and to fully assess the extent of current gene flow (nDNA) and reproductive exchange among selected wild populations of two species of carp (Henicorhynchus spp.) of high economic importance in the MRB using combined mtDNA and nDNA markers. In combination, the data can be used to define effective management units for each species. In general, nDNA diversity for H. lobatus (with average allelic richness (A) 7.56 and average heterozygosity (Ho) 0.61) was very similar to that identified for H. siamensis (A = 6.81 and Ho = 0.75). Both mud carp species show significant but low FST estimates among populations as a result of lower genetic diversity among sampled populations compared with genetic diversity within populations that may potentially mask any 'real' population structure. Overall, population genetic structure patterns from mtDNA and nDNA in both Henicorhynchus species were largely congruent. Different population structures however, were identified for the two Henicorhynchus species across the same geographical area. Apparent co-similarity in morphology and co-distribution of these two relatively closely related species does not apparently imply parallel evolutionary histories. Differences in each species population structure likely reflect historical drainage rearrangement of the Mekong River. The data indicate that H. siamensis is likely to have occupied the Mekong system for much longer than has H. lobatus in the past. Two divergent stocks were identified for H. lobatus in the MRB below the Khone Falls while a single stock had been evident in the earlier mtDNA study. This suggests that the two Henicorhynchus species may possess different life history traits and that different patterns of gene flow has likely influenced modern genetic structure in these close congeners. In combination, results of the earlier mtDNA and the current study have implications for effective management of both Henicorhynchus species across the MRB. Currently, both species are essentially treated as a single management unit in this region. This strategy may be appropriate for H. lobatus as a single stock was evident in the main stream of the MRB, but may not be appropriate for H. siamensis as more than a single stock was identified across the same range for this species. Management strategies should consider this difference to conserve overall biodiversity (local discrete populations) and this will include maintaining natural habitat and migration pathways, provision of fish sanctuaries (refuges) and may also require close monitoring of any stock declines, a signal that may require effective recovery strategies.

Values of inland fisheries in the Mekong river basin

Asia has the most productive inland fisheries in the world. The fishery sector contributes significantly to the national economies of the region. Inland fisheries also improve food security by providing a source of protein and a livelihood for millions of people in this part of the world, especially the rural poor. The purpose of this report is to provide information on the biological, economic, social and cultural values of river fisheries in the Lower Mekong Basin, and to identify the main impacts of environmental changes on these values. A review of fisheries-related literature, including project reports and gray literature, was undertaken. More than 800 documents were reviewed, and original information was extracted from 270 of them. The analysis identified a large number of localized studies leading to generic conclusions. The report addresses the basin wide issues and studies. It is then organized by nation, namely, the Chinese province of Yunnan, then Laos, Thailand, Cambodia and Vietnam. It first gives an overview of each country’s economic, fisheries and social situation, then details the values documented for river fisheries in each country.

Fish bioecology in relation to sediments in the Mekong and in tropical rivers

Sediments are an essential component of rivers and of their biological functioning. In addition to their influence on river geomorphology (maintenance of river forms and habitats such as pools and sand bars), sediments also include nutrients, detritus and organic debris of various sizes which interact with the river’s different life forms, including fish. The interaction between sediments and aquatic organisms, directly or indirectly through the effects of sediments on physical habitats, unquestionably influences the biodiversity and productivity of a river. The current report reviews the interactions between sediments and fish in tropical rivers and in the Mekong, and focuses more specifically on a reduction of sediment loads following dam const

Community management of Mekong River resources in Laos

The Mekong River is one of Asia's greatest rivers. It is the lifeblood of millions of small-scale farmers and fishers in China, Burma, Thailand, Laos, Cambodia and Vietnam. In the Lao People's Democratic Republic (Lao PDR), the river is particularly important because for the landlocked country, the Mekong is the Sea of Laos. In southern Lao PDR, fisheries for native fish species constitute an extremely important source of subsistence protein and income for local people. It has been estimated that wild-caught fish constitute at least 90% of the protein for lowland Laotians living next to the Mekong. Despite the extreme importance of fish to the diets and economics of communities along the Mekong, the resource, and the people who depend upon it, are in danger.

Production of freshwater prawns in the Mekong Delta

Currently there are 2 sources of freshwater prawn Macrobrachium rosenbergii production in Vietnam - natural production from the rivers and canals and aquaculture production from ponds and ricefields. An account is given of the production of this prawn in the Mekong Delta. The prawns are harvested primarily by a common small scale method using shelter traps. The total annual freshwater prawn production in Vietnam during 1985-90 was reported to vary from 5000 to 8000 tons, most of it from natural fisheries; although there are no official statistics on the production of farmed prawns, the amount is believed to be very small. The grow-out system includes intensive monospecies pond culture, and semi-intensive or extensive integrated rice-prawn or vegetable-prawn culture.

Fishes of the Mekong River: conservation and need for aquaculture

Conservation and the potential and need for aquaculture of three major fish (Catlocarpio siamensis, Probarbus spp. and Pangasius gigas) of the Mekong River are discussed. Different steps being undertaken by the Cambodian government in doing such activities are highlighted.

Rice-freshwater prawn (Macrobrachium rosenbergii) farms in the Mekong Delta, Vietnam

Aquaculture in riceland has been practiced in Mekong Delta, Vietnam for a long time and integrated rice-freshwater prawn (Macrobrachium rosenbergii) farming has become more and more popular. The integrated farming systems developed and practiced by farmers in the area to produce more food and more cash crops are presented and discussed.

Integrated rice-prawn farming in the Mekong Delta, Vietnam: a route towards sustainable and profitable farming systems?

Economic analysis and performance of the integrated rice-prawn farming systems in the Mekong Delta (Vietnam) are reviewed, including the problems and constraints of this integrated system technology.

Clam farming in the Mekong Delta, Vietnam

The Mekong Delta region in southern Vietnam has high potential for coastal aquaculture, including mollusc culture. Many mollusc species are cultured for domestic and export markets including white clam (Meretrix lyrata Showerby) and blood cockle (Arca granosa). Techniques for clam farming include the nursery and grow-out phases. At present, there are approximately 600 coastal families engaged in clam farming over a total area of 1,870 ha, of which 82.63% is used for the grow-out phased and 17.7% for the nursery phase. Nursery areas are near the coast and receive less than 5 hours of sunlight per day. The average area for a nursery is 3-4 ha and it is fenced with a net or bamboo stakes to prevent clams from escaping and to prevent water currents from carrying them away. Grow-out farm areas are further from the coast and are exposed to sunlight for only 2-3 hours/day. Average farm area for grow-out is 5-6 ha, and may or may not be fenced. Average operating cost is US$1100 per ha for nursery and US$757 per ha for grow-out (the cost of capital assets are not included) with loans being the main source of financial. Problems for clam farmers in the area include natural phenomena, inadequate culture techniques, lack of financing or credit systems, and marketing. Environment-related problems that cause clam mortality include flooding, and freshwater effluent and siltation or sedimentation from Mekong River. Other problems that constrain the development of clam culture in the area are: marketing problems such as lack of buyers and price fluctuations; exploitation of the natural clam populations.