1000 resultados para Magnetismo animal


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Precede al tit.: Faculté de Médecine de Paris.

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Índice.

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Este trabalho procura discutir o modo como os fenômenos sobrenaturais foram apropriados, pela ciência, no século XIX. A teoria do magnetismo animal, criada por Mesmer, com suas variadas interpretações por várias gerações de discípulos; a construção da teoria da hipnose, com a codificação da histeria abrindo definitivamente as portas das censuras acadêmicas; e a teoria da dissociação, criada no final daquele século, demonstram diferentes explicações fisicalistas que, muitas vezes, serviram para estabelecer distâncias entre um saber popular e o conhecimento de elites profissionais. A construção do cérebro possuído, no século XIX, apoiada na nosologia da histeria, codificada pela Escola de Salpêtrière, refletiu uma importante transformação social da época, em um processo de laicização da assistência pública, fundamental para a afirmação da psiquiatria como disciplina nascente. Atualmente, a codificação de fenômenos complexos, como transe e possessão espiritual que povoam a imaginação ou a superstição popular, ganha o estatuto de entidade nosológica, a partir das classificações diagnósticas oficiais da psiquiatria hegemônica. O cérebro será quase sempre a referência utilizada na esperança de naturalização do sobrenatural.

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A bioactive and bioresorbable scaffold fabricated from medical grade poly (epsilon-caprolactone) and incorporating 20% beta-tricalcium phosphate (mPCL–TCP) was recently developed for bone regeneration at load bearing sites. In the present study, we aimed to evaluate bone ingrowth into mPCL–TCP in a large animal model of lumbar interbody fusion. Six pigs underwent a 2-level (L3/4; L5/6) anterior lumbar interbody fusion (ALIF) implanted with mPCL–TCP þ 0.6 mg rhBMP-2 as treatment group while four other pigs implanted with autogenous bone graft served as control. Computed tomographic scanning and histology revealed complete defect bridging in all (100%) specimen from the treatment group as early as 3 months. Histological evidence of continuing bone remodeling and maturation was observed at 6 months. In the control group, only partial bridging was observed at 3 months and only 50% of segments in this group showed complete defect bridging at 6 months. Furthermore, 25% of segments in the control group showed evidence of graft fracture, resorption and pseudoarthrosis. In contrast, no evidence of graft fractures, pseudoarthrosis or foreign body reaction was observed in the treatment group. These results reveal that mPCL–TCP scaffolds could act as bone graft substitutes by providing a suitable environment for bone regeneration in a dynamic load bearing setting such as in a porcine model of interbody spine fusion.

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Conventional clinical therapies are unable to resolve osteochondral defects adequately, hence tissue engineering solutions are sought to address the challenge. A biphasic implant which was seeded with Mesenchymal Stem Cells (MSC) and coupled with an electrospun membrane was evaluated as an alternative. This dual phase construct comprised of a Polycaprolactone (PCL) cartilage scaffold and a Polycaprolactone - Tri Calcium Phosphate (PCL - TCP) osseous matrix. Autologous MSC was seeded into the entire implant via fibrin and the construct was inserted into critically sized osteochondral defects located at the medial condyle and patellar groove of pigs. The defect was resurfaced with a PCL - collagen electrospun mesh that served as a substitute for periosteal flap in preventing cell leakage. Controls either without implanted MSC or resurfacing membrane were included. After 6 months, cartilaginous repair was observed with a low occurrence of fibrocartilage at the medial condyle. Osteochondral repair was promoted and host cartilage degeneration was arrested as shown by the superior Glycosaminoglycan (GAG) maintenance. This positive morphological outcome was supported by a higher relative Young's modulus which indicated functional cartilage restoration. Bone in growth and remodeling occurred in all groups with a higher degree of mineralization in the experimental group. Tissue repair was compromised in the absence of the implanted cells or the resurfacing membrane. Moreover healing was inferior at the patellar groove as compared to the medial condyle and this was attributed to the native biomechanical features.