Commercial squids: Characterization, assessment of potential health benefits/risks and discrimination based on mineral, lipid and vitamin E concentrations

The most consumed squid species worldwide were characterized regarding their concentrations of minerals, fatty acids, cholesterol and vitamin E. Interspecific comparisons were assessed among species and geographical origin. The health benefits derived from squid consumption were assessed based on daily minerals intake and on nutritional lipid quality indexes. Squids contribute significantly to daily intake of several macro (Na, K, Mg and P) and micronutrients (Cu, Zn and Ni). Despite their low fat concentration, they are rich in long-chain omega-3 fatty acids, particularly docosahexaenoic (DHA) and eicosapentanoic (EPA) acids, with highly favorable ω-3/ω-6 ratios (from 5.7 to 17.7), reducing the significance of their high cholesterol concentration (140–549 mg/100 g ww). Assessment of potential health risks based on minerals intake, non-carcinogenic and carcinogenic risks indicated that Loligo gahi (from Atlantic Ocean), Loligo opalescens (from Pacific Ocean) and Loligo duvaucelii (from Indic Ocean) should be eaten with moderation due to the high concentrations of Cu and/or Cd. Canonical discriminant analysis identified the major fatty acids (C14:0, C18:0, C18:1, C18:3ω-3, C20:4ω-6 and C22:5ω-6), P, K, Cu and vitamin E as chemical discriminators for the selected species. These elements and compounds exhibited the potential to prove authenticity of the commercially relevant squid species.

Functional components and lipid quality assessment in vegetable foods: analytical and technological approaches

Lipids are important components that contribute very significantly to nutritional and technological quality of foods because they are the least stable macro-components in foods, due to high susceptibility to oxidation. When rancidity take place, it makes food unhealthy and unacceptable for consumers. Thus, the presence of antioxidants, naturally present of added to foods, is required to enhance shelf life of foods. Moreover, antioxidant like phenolic compounds play an important role in human health enhancing the functionality of foods. The aim of this PhD project was the study of lipid quality and lipid oxidation in different vegetable foods focusing on analytical and technological aspects in order to figure out the effects of lipid composition and bioactive compounds (phenolic compounds, omega-3 fatty acids and dietary fiber) addition on their shelf life. In addition, bioavailability and antioxidant effects of phenolic compounds in human and animals, respectively, were evaluated after consumption of vegetable foods. The first section of the work was focused on the evaluation of lipid quality impact on technological behaviour of vegetable foods. Because of that, cocoa butter with different melting point were evaluated by chromatographic techniques (GC, TLC) and the sample with the higher melting point showed the presence of fatty acids, triglycerides, 2-monoglycerides and FT-IR profile different from genuine cocoa butter, meaning an adding of foreign fat (lauric-fat) not allowed by the law. Looking at lipid quality of other vegetable foods, an accelerated shelf life test (OXITEST®), was used to evaluate of lipid stability to oxidation in tarallini snacks made up using different lipid matrices (sunflower oil, extravirgin olive oil and a blend of extravirgin olive oil and lard). The results showed a good ability of OXITEST® to discriminate between lipid unsaturation and different cooking times, without any samples fat extraction. In the second section, the role of bioactive compounds on cereal based food shelf life was studied in different bakeries by GC, spectrophotometric methods and capillary electrophoresis. It was examined the relationships between phenolic compounds, added with flour, and lipid oxidation of tarallini and frollini. Both products showed an increase in lipid oxidation during storage and antioxidant effects on lipid oxidation were not as expected. Furthermore, the influence of enrichment in polyunsaturated fatty acids on lipid oxidation of pasta was evaluated. The results proved that LC n-3 PUFA were not significantly implicated in the onset of oxidation in spaghetti stored under daylight and accelerated oxidation in a laboratory heater. The importance of phenolic compounds as antioxidant in humans and rats was also studied, by HPLC/MS in the latter section. For this purpose, apigenin and apigenin glycosides excretion was investigated in six women’s urine in a 24 hours study. After a single dose of steamed artichokes, both aglicone and glucuronide metabolites were recovered in 24 h urine. Moreover, the effect of whole grain durum wheat bread and whole grain Kamut® khorasan bread in rats were evaluated. Both cereals were good sources of antioxidants but Kamut® bread fed animals had a better response to stress than wheat durum fed, especially when a sourdough bread was supplied.

Lipid quality and oxidative stability in food products from animal origin, as affected by breeding factor

Nowadays it is requested more investigations on alternative rearing systems that are able to improve poultry welfare and to warrant high-quality and safe meat products. This thesis work was focused on the evaluation of the oxidative stability of poultry meats, obtained with different rearing systems, diets (supplemented with bioactive compounds), and packaging conditions. The thesis work was divided into the following parts: - Evaluation of the effects of different rearing systems on the quality, fatty acid composition and oxidative stability of poultry thigh and breast meat belonging to different product categories (“rotisserie” and “cut-up” carcasses); - Evaluation of the effects of different rearing systems and packaging conditions on the shelf-life of poultry thigh meat stored at 4°C for 14 days, and the effects of feed supplementation with thymol (control diet and diet with 2 different concentration of thymol) and packaging conditions on lipid oxidation of poultry thigh meat shelf-life (stored at 4°C for 14 days). The oxidative stability of poultry meat was studied by means of the spectrophotometric determinations of peroxide value and thiobarbituric acid reactive substances. - Evaluation of anti-inflammatory effects of different flavonoids (thymol, luteolin, tangeretin, sulforaphane, polymethoxyflavones, curcumin derivates) to detect their biological activity in LPS-stimulated RAW 264.7 macrophage cells in vitro, in order to study more in depth their action mechanisms. It was evaluated the cell vitality (MTT assay), nitrite concentration and protein profile. The study was focused on the identification of potential dietary bioactive compounds in order to investigate their biological activity and possible synergic effects, and to develop new suitable strategies for long-term promotion of human health, in particular against cancer.

Produção de lipidios funcionais por ação de lipase fúngica

A modificação estrutural de óleos e gorduras é uma das principais áreas de interesse de pesquisa em diferentes setores industriais. No caso da indústria de alimentos, a interesterificação é empregada para melhorar propriedades nutricionais e funcionais, em que se obtêm compostos diferentes dos que lhes deram origem. As lipases microbianas são os biocatalisadores mais utilizados industrialmente, por serem mais estáveis, específicas e com propriedades bem mais diversificadas que as lipases de outras fontes. Este trabalho objetivou, primeiramente, a caracterização da gordura da pele de frango (GPF) e sua comparação com óleo de soja, como referência, visando a utilização de GPF em reações de interesterificação. Para isto foram caracterizados quanto aos índices de rancidez hidrolítica e oxidativa, bem como de matéria insaponificável, índices de saponificação, refração e iodo. Foi realizado ainda o fracionamento e perfil de ácidos graxos destes lipídios e suas frações, com o cálculo de seus índices nutricionais. Foi verificado que a GPF apresentou qualidade satisfatória devido aos baixos índices de acidez (0,65 g ácido oleico.100 g -1 ), peróxido (2,14 meq.kg-1 ), p-anisidina (0,70 unidades de absorvância.g-1 ), além de fonte de ácidos graxos mono-insaturados (40%), sendo fonte promissora para estudos de interesterificação. Em um segundo momento o objetivo foi produzir lipídios modificados ricos em ácidos graxos essenciais a partir da gordura da pele de frango e ácidos graxos ramificados, utilizando lipase sn-1,3 específica e interesterificação do tipo acidólise. Foram estudados os fatores concentração de enzima, adição de água, proporção de substratos e tempo, segundo um planejamento experimental fatorial completo 2 4 . As separações analíticas foram executadas em placas de cromatografia de camada delgada, sendo as frações posteriormente extraídas, ressuspensas e injetadas no cromatógrafo a gás. Foi verificado que a adição de água ao meio reacional apresentou efeito significativo (p<0,05) para todos ácidos graxos avaliados dos triacilgliceróis, sendo que para o ácido essencial linoleico (C18:2) o efeito do tempo de reação também foi significativo, sendo verificado que quanto maior o tempo de reação, menor a quantidade de água a ser adicionada. Em um terceiro momento, o objetivo foi produzir éster fenólico a partir do DHCA, além de realizar reações de transesterificação deste éster com tricaprilina. Para a reação de transesterificação, foi utilizado um delineamento composto central rotacional (DCCR) variando a quantidade de enzima, tempo de reação e temperatura sobre a resposta (%) dos reagentes consumidos. A lipase Novozym® 435 de Candida antarctica foi utilizada como catalisador de todas reações. Foi verificado que a maior produção de éster (50%) ocorreu em oito dias. Nas reações de transesterificação, as relações molares em que houve maior consumo do éster produzido foram 1:5 e 1:10, sendo obtidos 21,1% e 29,6% de residual de dihidrocafeato de octila, respectivamente em 24 h. Foi observado que em altas temperaturas e tempo superior a 26 h, houve o menor residual de dihidrocafeato de octila (18,2%). Foram identificados três diferentes compostos fenólicos, contendo em sua estrutura dihidrocafeato de octila e ácido caprílico.

Soil quality assessment through a multi-approach analysis in soils of abandoned terraced land in NE Spain

The abandonment of agricultural land in mountainous areas has been an outstanding problem along the last century and has captured the attention of scientists, technicians and administrations, for the dramatic consequences sometimes occurred due to soil instability, steep slopes, rainfall regimes and wildfires. Hidromorfological and pedological alterations causing exceptional floods and accelerated erosion processes has therefore been studied, identifying the cause in the loss of landscape heterogeneity. Through the disappearance of agricultural works and drainage maintenance, slope stability has resulted severely affected. The mechanization of agriculture has caused the displacement of vines, olives and corks trees cultivation in terraced areas along the Mediterranean catchment towards more economically suitable areas. On the one hand, land use and management changes have implicated sociological changes as well, transforming areas inhabited by agricultural communities into deserted areas where the colonization of disorganized spontaneous vegetation has buried a valuable rural patrimony. On the other hand, lacking of planning and management of the abandoned areas has produced badlands and infertile soils due to wildfire and high erosion rates strongly degrading the whole ecosystems. In other cases, after land abandonment a process of soil regeneration has been recorded. Investigations have been conducted in a part of NE Spain where extended areas of terraced soils previously cultivated have been abandoned in the last century. The selected environments were semi-abandoned vineyards, semi-abandoned olive groves, abandoned stands of cork trees, abandoned stands of pine trees, scrubland of Cistaceaea, scrubland of Ericaceaea, and pasture. The research work was focused on the study of most relevant physical, chemical and biological soil properties, as well as runoff and erosion under soils with different plant cover to establish the abandonment effect on soil quality, due to the peculiarity and vulnerability of these soils with a much reduced depth. The period of observation was carried out from autumn 2009 to autumn 2010. The sediment concentration of soil erosion under vines was recorded as 34.52 g/l while under pasture it was 4.66 g/l. In addition, the soil under vines showed the least amount of organic matter, which was 12 times lower than all other soil environments. The carbon dioxide (CO2) and total glomalin (TG) ratio to soil organic carbon (SOC) in this soil was 0.11 and 0.31 respectively. However, the soil under pasture contained a higher amount of organic matter and showed that the CO2 and TG ratio to SOC was 0.02 and 0.11 respectively indicating that the soil under pasture better preserves the soil carbon pool. A similar trend was found in the intermediate soils in the sequence of land use change and abandonment. Soil structural stability increased in the two soil fractions investigated (0.25-2.00 mm, 2.0-5.6 mm) especially in those soils that did not undergo periodical perturbations like wildfires. Soil quality indexes were obtained by using relevant physical and chemical soil parameters. Factor analysis carried out to study the relationship between all soil parameters allowed to related variables and environments and identify those areas that better contribute to soil quality towards others that may need more attention to avoid further degradation processes

Development of a water quality index using a fuzzy logic: A case study for the sorocaba river

Due to growing urbanization and industrialization, the environment is suffering from pollution of rivers, degradation of soils and deteriorated air quality. Quality indices appear to be useful to evaluate the conditions of these media. The aim of this study was the development of a water quality index using a fuzzy inference system, since such an approach has proved advantageous in addressing problems that are subjective by nature or for which the data are uncertain. The methodology employed was based on this inference system, and considered the nine water quality parameters employed by CETESB (Companhia de Tecnologia de Saneamento Ambiental, São Paulo, Brazil) to evaluate water quality. After assessment of the data using the index, a comparison was made with the WQI (Water Quality Index), which is used for the monitoring of various water bodies, including in the study region. The results obtained using the index developed on the basis of fuzzy inference were found to be more useful than those derived from the method currently used by CETESB, since losses and/or omissions concerning individual parameters were minimized. © 2010 IEEE.

Óleo de linhaça na terminação de novilhos Nelore: qualidade da carne

Pós-graduação em Zootecnia - FCAV

Harvesting and wood transport planning with SNAP III program (Scheduling and Network Analysis Program) in a pine plantation in Southeast Brazil

The objective of this study was to verify the potential of SNAP III (Scheduling and Network Analysis Program) as a support tool for harvesting and wood transport planning in Brazil harvesting subsystem definition and establishment of a compatible route were assessed. Initially, machine operational and production costs were determined in seven subsystems for the study area, and quality indexes, construction and maintenance costs of forest roads were obtained and used as SNAP III program input data. The results showed, that three categories of forest road occurrence were observed in the study area: main, secondary and tertiary which, based on quality index, allowed a medium vehicle speed of about 41, 30 and 24 km/hours and a construction cost of about US$ 5,084.30, US$ 2,275.28 and US$ 1,650.00/km, respectively. The SNAP III program used as a support tool for the planning, was found to have a high potential tool in the harvesting and wood transport planning. The program was capable of defining efficiently, the harvesting subsystem on technical and economical basis, the best wood transport route and the forest road to be used in each period of the horizon planning.

Spatial influence of physical and chemical parameters on management zone definition in apple orchards

ABSTRACT Precision agriculture adoption in Brazilian apple orchards is still incipient. This study aimed at evaluating the spatial variability of certain soil properties as soil density, soil penetration resistance, electrical conductivity, yield, and fruit quality in an apple orchard through digital mapping, as well as assessing the correlation between these factors by means of geostatistics, establishing management zones. Forty representative points were set within 2.5 hectares of apple orchard, wherein soil samples were collected and analyzed, besides measurements of fruit quality (Brix degree, size or diameter, pulp firmness and color) to generate an overall index quality. We concluded that the fruit quality indexes, when isolated, did not show strong spatial dependence, unlike the index of fruit quality (FQI), derived from a combination of these parameters, allowing orchard planning according to management zones based on quality.

Informática na EJA: contribuições da teoria histórico-cultural

Pós-graduação em Educação - FFC

Caracterização limnológica e microbiológica do córrego rico que abastece Jaboticabal (SP)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Qualidade de vida em Porto Velho, Rondônia: perspectivas do processo de desenvolvimento regional

Esta tese de doutorado nasceu da escassez de estudos referentes aos efeitos das grandes obras, sobre a qualidade de vida das cidades. Nesse sentido, visando estudar exatamente esse problema dentro de um cenário amazônico, propôs-se pesquisar os efeitos das usinas hidrelétricas de Jirau e de Santo Antônio, em seu processo de construção, às margens do rio Madeira, em Rondônia e, assim, analisar a qualidade de vida dentro do perímetro urbano da cidade de Porto Velho, o que constituiu o objeto de estudo da presente investigação. Para tanto, buscou-se aporte na teoria do “Fetiche”, de Marx, na obra “O capital” (1980). Assim, procurou-se explicar a migração de trabalhadores, na busca por melhores salários e oportunidades de ganhos econômicos, nas obras das hidrelétricas mencionadas. A perspectiva teórica do “fetichismo da mercadoria”, de Marx (1967), desencadeou a inter-relação com os aspectos diretamente ligados ao âmago da presente tese, que se constitui numa Pesquisa de natureza qualitativa. Essa foi desenvolvida com base no método de Análise Fatorial, utilizando o programa estatístico SPSS, versão 17. Para isso, foram aplicados 1.449 questionários, distribuídos igualitariamente, em grupos de 21, para cada um dos 69 bairros da cidade. E, dessa forma, construir os índices de IQVU (índices de qualidade de vida urbana), segundo Santos e Martins (2002) e, para análises multifatoriais, de acordo com Hair et al. (2005) e com Santana (2007; 2005). A partir disso, os resultados obtidos permitiram concluir que o índice de qualidade de vida de Porto Velho, no período correspondente à fase anterior a chegada das usinas, à região, era de 0,452. Portanto, classificado como um índice de desempenho regular. Em relação à fase atual, esse índice apresentou uma queda de 15,31%, alcançando um valor de 0,392. Por consequência, considerado de ruim desempenho. Desse modo, permitiu-se concluir que a qualidade de vida de Porto Velho, conforme a opinião dos entrevistados, literalmente, piorou com a chegada das usinas hidrelétricas do rio Madeira, em Porto Velho/Rondônia. A relevância do presente estudo reside na análise do espaço intramunicipal e na integração dos dados provenientes da pesquisa de campo, com a informação gerada durante as análises. Isto levou a deduções lógicas, fundamentadas nos critérios adotados e decorrentes dos resultados das avaliações. Consequentemente, esse poderá servir como suporte às decisões políticas, sobre o planejamento de ações a serem executadas, para gestão do território no âmbito municipal.

Qualidade de vida do trabalhador de enfermagem de um hospital Estadual

The search for quality of life (QOL) is now an ideal among thousands of people worldwide and is currently the subject has been studied in different areas of knowledge. Thus, one can perceive it in different dimensions, each with its due importance to people's lives. The verification of quality indexes of work life (QWL) may provide information on factors that directly interfere with the satisfaction and personal motivation and collective, with reflections on the structure and excellent service. To this end, we carried out a study to know the characacteristics of quality of life of nursing professionals in a state hospital in São Paulo State, based on short range of quality of life of the World Health Organization (WHOQOL-BREF). This work is a study with a quantitative approach, cross-sectional descriptive and exploratory data analysis using descriptive statistics, involving a sample of 281 nurses who answered the scale for measuring quality of life, composed of four areas: physical, psychological, social relationships and environment. In the study, these areas were related to the professional position, and shift work. There was a satisfactory quality of life in the study population in different areas of WHOQOL-BREF. There was no statistically significant changes in quality of life among the variables. It follows that the population presented data consistent with an adequate quality of life, thus making relevant to addressing the quality of life of nursing in a healthcare organization which has as fundamental to the pursuit of quality through the accreditation process second hospital established by the National Accreditation Organization (ONA) that aims at establishing and implementing a process of improving health care, thereby stimulating the services to achieve higher standards of quality

Conservación de dos variedades de arándano alto en condiciones de frío convencional

Se evaluaron dos cultivares de arándano alto por su comportamiento en almacenamiento refrigerado convencional. Frutos completamente azules se seleccionaron, envasaron en "clamshells" y sometieron a almacenamiento refrigerado a 0 °C y 85-90 % HR durante 10, 17, 24 y 31 días. Para cada período de almacenamiento se realizaron muestreos: (1) a salida de frío y (2) después de dos días a temperatura ambiente. Se evaluó la homogeneidad en el empaque, pérdida de peso, firmeza, contenido de sólidos solubles, acidez titulable, pH y relación sólidos solubles:acidez titulable. El peso promedio de los frutos fue de 1.9 g, correspondiéndoles el grado "extralarge". La fruta se mantuvo en buenas condiciones durante 11 días de almacenamiento refrigerado, después perdió calidad como consecuencia de la pérdida de peso. Se encontró que Bluejay superó a Brigitta en firmeza. El contenido de sólidos solubles aumentó proporcionalmente a la duración del período de almacenamiento. En los períodos de 10 y 31 días Brigitta presentó mayor acidez titulable y menor relación sólidos solubles:acidez titulable que Bluejay. En ambos cultivares se observó un aumento significativo del pH al mes de almacenamiento. A partir de la correlación entre las variables evaluadas se infiere que el contenido de sólidos solubles y la firmeza constituyeron buenos índices de la calidad de la fruta en ambas variedades.

Determinación de índices de cosecha y calidad en kiwi en el sudeste bonaerense (Argentina)

Se realizó un seguimiento de la maduración de kiwis (Actinidia celiciosa (A. Chev.) Liang et Ferguson cv. Hayward) en una plantación comercial del sudeste de la provincia de Buenos Aires (Argentina), con la finalidad de definir índices de madurez y calidad y compararlos con los estándares vigentes en Nueva Zelanda y Chile. A intervalo semanal, desde las 13 semanas después de plena floración, se tomaron muestras de frutos para evaluar las siguientes variables: peso fresco, volumen, porcentaje de semillas negras, contenido de materia seca, acidez titulable y pH. De acuerdo con los mismos, el valor mínimo de sólidos solubles y el porcentaje de semillas negras exigido se alcanzaron durante la semana 21 después de plena floración y la firmeza de pulpa presentaba aún valores superiores al umbral recomendado. El momento óptimo de cosecha correspondió a plena temporada. Los resultados sugieren que la acidez titulable podría constituir otro índice de madurez. El porcentaje de materia seca no sería útil en fruta de plena temporada dado que los incrementos significativos se registran tempranamente. Sin embargo, puede ser útil como índice de calidad. Se plantea la posibilidad de utilizar la relación MS/AT como índice de calidad y de cosecha.