Search for new light gauge bosons in Higgs boson decays to four-lepton final states in pp collisions at s√=8TeV with the ATLAS detector at the LHC

This paper presents a search for Higgs bosons decaying to four leptons, either electrons or muons, via one or two light exotic gauge bosons Zd, H→ZZd→4ℓ or H→ZdZd→4ℓ. The search was performed using pp collision data corresponding to an integrated luminosity of about 20 fb−1 at the center-of-mass energy of s√=8TeV recorded with the ATLAS detector at the Large Hadron Collider. The observed data are well described by the Standard Model prediction. Upper bounds on the branching ratio of H→ZZd→4ℓ and on the kinetic mixing parameter between the Zd and the Standard Model hypercharge gauge boson are set in the range (1--9)×10−5 and (4--17)×10−2 respectively, at 95% confidence level assuming the Standard Model branching ratio of H→ZZ∗→4ℓ, for Zd masses between 15 and 55 GeV. Upper bounds on the effective mass mixing parameter between the Z and the Zd are also set using the branching ratio limits in the H→ZZd→4ℓ search, and are in the range (1.5--8.7)×10−4 for 15

Solução construtiva com elementos de aço enformados a frio

A atual dissertação consiste numa apreciação global dos vários critérios que integram a aplicação do método construtivo de elementos estruturais de aço enformado a frio, muitas vezes designado por método prescritivo da construção em “aço leve”, “Light Steel Framing” (LSF) ou em alternativa também designado de “Light Gauge Steel Framing” (LGSF) e está especialmente vocacionado para a construção de edifícios de um a três pisos. Este conceito tem origem no facto de se usar chapas de aço de espessura reduzida, respetivamente mais leve, para fabricação dos perfis o que contribui para um menor peso dos elementos estruturais. Será abordado essencialmente o sector dos edifícios de habitação bem como a reabilitação dos mesmos, com recurso à solução em estudo, dado que é um dos setores que detém maior impacte socio-económico e ambiental. O estudo engloba igualmente a descrição e análise dos métodos construtivos, bem como os produtos empregues nesta solução sustentável, definindo desta forma uma resposta ajustada a cada subsistema da construção, desde estrutura, pavimentos, coberturas, fachadas, divisórias, climatização e acústica. Por fim, para obter um retrato da prática na Região Autónoma da Madeira, face a este tipo de construção sustentável, realizou-se uma análise de casos de estudo, nomeadamente no que diz respeito à viabilidade económica desta solução construtiva.

Design of sway frames

The aim of the work presented in this thesis is to produce a direct method to design structures subject to deflection constraints at the working loads. The work carried out can be divided into four main parts. In the first part, a direct design procedure for plane steel frames subjected to sway limitations is proposed. The stiffness equations are modified so that the sway in each storey is equal to some specified values. The modified equations are then solved by iteration to calculate the cross-sectional properties of the columns as well as the other joint displacements. The beam sections are selected initially and then altered in an effort to reduce the total material cost of the frame. A linear extrapolation technique is used to reduce this cost. In this design, stability functions are used so that the effect of axial loads in the members are taken into consideration. The final reduced cost design is checked for strength requirements and the members are altered accordingly. In the second part, the design method is applied to the design of reinforced concrete frames in which the sway in the columns play an active part in the design criteria. The second moment of area of each column is obtained by solving the modified stiffness equations and then used to calculate the mlnlmum column depth required. Again the frame has to be checked for all the ultimate limit state load cases. In the third part, the method is generalised to design pin-jointed space frames for deflection limitatlions. In these the member areas are calculated so that the deflection at a specified joint is equal to its specified value. In the final part, the Lagrange multiplier technique is employed to obtain an optimum design for plane rigidly jointed steel frames. The iteration technique is used here to solve the modified stiffness equations as well as derivative equations obtained in accordance to the requirements of the optimisation method.

Interactions between Spider Silk and Cells - NIH/3T3 Fibroblasts Seeded on Miniature Weaving Frames

Background: Several materials have been used for tissue engineering purposes, since the ideal matrix depends on the desired tissue. Silk biomaterials have come to focus due to their great mechanical properties. As untreated silkworm silk has been found to be quite immunogenic, an alternative could be spider silk. Not only does it own unique mechanical properties, its biocompatibility has been shown already in vivo. In our study, we used native spider dragline silk which is known as the strongest fibre in nature. Methodology/Principal Findings: Steel frames were originally designed and manufactured and woven with spider silk, harvesting dragline silk directly out of the animal. After sterilization, scaffolds were seeded with fibroblasts to analyse cell proliferation and adhesion. Analysis of cell morphology and actin filament alignment clearly revealed adherence. Proliferation was measured by cell count as well as determination of relative fluorescence each after 1, 2, 3, and 5 days. Cell counts for native spider silk were also compared with those for trypsin-digested spider silk. Spider silk specimens displayed less proliferation than collagen-and fibronectin-coated cover slips, enzymatic treatment reduced adhesion and proliferation rates tendentially though not significantly. Nevertheless, proliferation could be proven with high significance (p<0.01). Conclusion/Significance: Native spider silk does not require any modification to its application as a biomaterial that can rival any artificial material in terms of cell growth promoting properties. We could show adhesion mechanics on intracellular level. Additionally, proliferation kinetics were higher than in enzymatically digested controls, indicating that spider silk does not require modification. Recent findings concerning reduction of cell proliferation after exposure could not be met. As biotechnological production of the hierarchical composition of native spider silk fibres is still a challenge, our study has a pioneer role in researching cellular mechanics on native spider silk fibres.

Paperikoneen viira- ja puristinosan mitoituslaskennan ohjeistaminen

Työssä laadittiin viira- ja puristinosan rungoston mitoituslaskentaa varten ohjeet FE-analysointiin. Työ suoritettiin keräämällä aluksi eri yksiköissä Valmetilla käytössä olevat mitoituslaskentaohjeet, aihetta käsittelevät muistiot ja diplomityöt sekä sekä keskustelemalla muiden viira- ja puristinosastolla laskentaa suorittavien suunnittelijoiden kanssa. Tämän jälkeen ohjeista koottiin ja täydennettiin yksi yhteinen ohjeisto, joka on jatkossa kaikkien laskijoiden käytössä. Valmiit ohjeet koottiin Valmetin Rautpohjan osastoiden yhteiseen Notes- järjestelmään. Ohjekannasta tehtiin sellainen, että siihen on helppo myöhemmin lisätä muiden rakenneryhmien ohjeita. Ohjeet jaettiin viiteen ryhmään ohjeiden lukemisen ja päivityksen helpottamiseksi. Mitoituslaskentaohjeiden käyttöä testattiin laskemalla yhden todellisen projektin puristinosan runkolaskelmat ohjeiden avulla. Yhteisen ohjeistuksen avulla yhtenäistettiin mallinnus- ja laskentatapoja niin Valmetin sisällä kuin mitoituslaskentaa tekevien alihankkijoiden keskuudessa.

Search for Dark Photons from Supersymmetric Hidden Valleys

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Sulla progettazione in zona sismica di strutture dotate di dissipatori fluido-viscosi: proposta di una metodologia

CHAPTER 1:FLUID-VISCOUS DAMPERS In this chapter the fluid-viscous dampers are introduced. The first section is focused on the technical characteristics of these devices, their mechanical behavior and the latest evolution of the technology whose they are equipped. In the second section we report the definitions and the guide lines about the design of these devices included in some international codes. In the third section the results of some experimental tests carried out by some authors on the response of these devices to external forces are discussed. On this purpose we report some technical schedules that are usually enclosed to the devices now available on the international market. In the third section we show also some analytic models proposed by various authors, which are able to describe efficiently the physical behavior of the fluid-viscous dampers. In the last section we propose some cases of application of these devices on existing structures and on new-construction structures. We show also some cases in which these devices have been revealed good for aims that lies outside the reduction of seismic actions on the structures. CHAPTER 2:DESIGN METHODS PROPOSED IN LITERATURE In this chapter the more widespread design methods proposed in literature for structures equipped by fluid-viscous dampers are introduced. In the first part the response of sdf systems in the case of harmonic external force is studied, in the last part the response in the case of random external force is discussed. In the first section the equations of motion in the case of an elastic-linear sdf system equipped with a non-linear fluid-viscous damper undergoing a harmonic force are introduced. This differential problem is analytically quite complex and it’s not possible to be solved in a closed form. Therefore some authors have proposed approximate solution methods. The more widespread methods are based on equivalence principles between a non-linear device and an equivalent linear one. Operating in this way it is possible to define an equivalent damping ratio and the problem becomes linear; the solution of the equivalent problem is well-known. In the following section two techniques of linearization, proposed by some authors in literature, are described: the first technique is based on the equivalence of the energy dissipated by the two devices and the second one is based on the equivalence of power consumption. After that we compare these two techniques by studying the response of a sdf system undergoing a harmonic force. By introducing the equivalent damping ratio we can write the equation of motion of the non-linear differential problem in an implicit form, by dividing, as usual, for the mass of the system. In this way, we get a reduction of the number of variables, by introducing the natural frequency of the system. The equation of motion written in this form has two important properties: the response is linear dependent on the amplitude of the external force and the response is dependent on the ratio of the frequency of the external harmonic force and the natural frequency of the system only, and not on their single values. All these considerations, in the last section, are extended to the case of a random external force. CHAPTER 3: DESIGN METHOD PROPOSED In this chapter the theoretical basis of the design method proposed are introduced. The need to propose a new design method for structures equipped with fluid-viscous dampers arises from the observation that the methods reported in literature are always iterative, because the response affects some parameters included in the equation of motion (such as the equivalent damping ratio). In the first section the dimensionless parameterε is introduced. This parameter has been obtained from the definition of equivalent damping ratio. The implicit form of the equation of motion is written by introducing the parameter ε, instead of the equivalent damping ratio. This new implicit equation of motions has not any terms affected by the response, so that once ε is known the response can be evaluated directly. In the second section it is discussed how the parameter ε affects some characteristics of the response: drift, velocity and base shear. All the results described till this point have been obtained by keeping the non-linearity of the behavior of the dampers. In order to get a linear formulation of the problem, that is possible to solve by using the well-known methods of the dynamics of structures, as we did before for the iterative methods by introducing the equivalent damping ratio, it is shown how the equivalent damping ratio can be evaluated from knowing the value of ε. Operating in this way, once the parameter ε is known, it is quite easy to estimate the equivalent damping ratio and to proceed with a classic linear analysis. In the last section it is shown how the parameter ε could be taken as reference for the evaluation of the convenience of using non-linear dampers instead of linear ones on the basis of the type of external force and the characteristics of the system. CHAPTER 4: MULTI-DEGREE OF FREEDOM SYSTEMS In this chapter the design methods of a elastic-linear mdf system equipped with non-linear fluidviscous dampers are introduced. It has already been shown that, in the sdf systems, the response of the structure can be evaluated through the estimation of the equivalent damping ratio (ξsd) assuming the behavior of the structure elastic-linear. We would to mention that some adjusting coefficients, to be applied to the equivalent damping ratio in order to consider the actual behavior of the structure (that is non-linear), have already been proposed in literature; such coefficients are usually expressed in terms of ductility, but their treatment is over the aims of this thesis and we does not go into further. The method usually proposed in literature is based on energy equivalence: even though this procedure has solid theoretical basis, it must necessary include some iterative process, because the expression of the equivalent damping ratio contains a term of the response. This procedure has been introduced primarily by Ramirez, Constantinou et al. in 2000. This procedure is reported in the first section and it is defined “Iterative Method”. Following the guide lines about sdf systems reported in the previous chapters, it is introduced a procedure for the assessment of the parameter ε in the case of mdf systems. Operating in this way the evaluation of the equivalent damping ratio (ξsd) can be done directly without implementing iterative processes. This procedure is defined “Direct Method” and it is reported in the second section. In the third section the two methods are analyzed by studying 4 cases of two moment-resisting steel frames undergoing real accelerogramms: the response of the system calculated by using the two methods is compared with the numerical response obtained from the software called SAP2000-NL, CSI product. In the last section a procedure to create spectra of the equivalent damping ratio, affected by the parameter ε and the natural period of the system for a fixed value of exponent α, starting from the elasticresponse spectra provided by any international code, is introduced.

The influence of infill walls on seismic behavior of a historical building in Shanghai

Seismic assessment and seismic strengthening are the key issues need to be figured out during the process of protection and reusing of historical buildings. In this thesis the seismic behaviors of the hinged steel structure, a typical structure of historical buildings, i.e. hinged steel frames in Shanghai, China, were studied based on experimental investigations and theoretic analysis. How the non-structural members worked with the steel frames was analyzed thoroughly. Firstly, two 1/4 scale hinged steel frames were constructed based on the structural system of Bund 18, a historical building in Shanghai: M1 model without infill walls, M2 model with infill walls, and tested under the horizontal cyclic loads to investigate their seismic behavior. The Shaking Table Test and its results indicated that the seismic behavior of the hinged steel frames could be improved significantly with the help of non-structural members, i.e., surrounding elements outside the hinged steel frames and infilled walls. To specify, the columns are covered with bricks, they consist of I shape formed steel sections and steel plates, which are clenched together. The steel beams are connected to the steel column by steel angle, thus the structure should be considered as a hinged frame. And the infilled wall acted as a compression diagonal strut to withstand the horizontal load, therefore, the seismic capacity and stiffness of the hinged steel frames with infilled walls could be estimated by using the equivalent compression diagonal strut model. A SAP model has been constructed with the objective to perform a dynamic nonlinear analysis. The obtained results were compared with the results obtained from Shaking Table Test. The Test Results have validated that the influence of infill walls on seismic behavior can be estimated by using the equivalent diagonal strut model.

Inestabilidad de pórticos planos metálicos de edificación con inclusión de la flexibilidad de las uniones y elementos no prismáticos : un enfoque para la optimización

En esta tesis se aborda el problema de la modelización, análisis y optimización de pórticos metálicos planos de edificación frente a los estados límites último y de servicio. El objetivo general es presentar una técnica secuencial ordenada de optimización discreta para obtener el coste mínimo de pórticos metálicos planos de edificación, teniendo en cuenta las especificaciones del EC-3, incorporando las uniones semirrígidas y elementos no prismáticos en el proceso de diseño. Asimismo se persigue valorar su grado de influencia sobre el diseño final. El horizonte es extraer conclusiones prácticas que puedan ser de utilidad y aplicación simple para el proyecto de estructuras metálicas. La cantidad de publicaciones técnicas y científicas sobre la respuesta estructural de entramados metálicos es inmensa; por ello se ha hecho un esfuerzo intenso en recopilar el estado actual del conocimiento, sobre las líneas y necesidades actuales de investigación. Se ha recabado información sobre los métodos modernos de cálculo y diseño, sobre los factores que influyen sobre la respuesta estructural, sobre técnicas de modelización y de optimización, al amparo de las indicaciones que algunas normativas actuales ofrecen sobre el tema. En esta tesis se ha desarrollado un procedimiento de modelización apoyado en el método de los elementos finitos implementado en el entorno MatLab; se han incluido aspectos claves tales como el comportamiento de segundo orden, la comprobación ante inestabilidad y la búsqueda del óptimo del coste de la estructura frente a estados límites, teniendo en cuenta las especificaciones del EC-3. También se ha modelizado la flexibilidad de las uniones y se ha analizado su influencia en la respuesta de la estructura y en el peso y coste final de la misma. Se han ejecutado algunos ejemplos de aplicación y se ha contrastado la validez del modelo con resultados de algunas estructuras ya analizadas en referencias técnicas conocidas. Se han extraído conclusiones sobre el proceso de modelización y de análisis, sobre la repercusión de la flexibilidad de las uniones en la respuesta de la estructura. El propósito es extraer conclusiones útiles para la etapa de proyecto. Una de las principales aportaciones del trabajo en su enfoque de optimización es la incorporación de una formulación de elementos no prismáticos con uniones semirrígidas en sus extremos. Se ha deducido una matriz de rigidez elástica para dichos elementos. Se ha comprobado su validez para abordar el análisis no lineal; para ello se han comparado los resultados con otros obtenidos tras aplicar otra matriz deducida analíticamente existente en la literatura y también mediante el software comercial SAP2000. Otra de las aportaciones de esta tesis es el desarrollo de un método de optimización del coste de pórticos metálicos planos de edificación en el que se tienen en cuenta aspectos tales como las imperfecciones, la posibilidad de incorporar elementos no prismáticos y la caracterización de las uniones semirrígidas, valorando la influencia de su flexibilidad sobre la respuesta de la estructura. Así, se han realizado estudios paramétricos para valorar la sensibilidad y estabilidad de las soluciones obtenidas, así como rangos de validez de las conclusiones obtenidas. This thesis deals with the problems of modelling, analysis and optimization of plane steel frames with regard to ultimate and serviceability limit states. The objective of this work is to present an organized sequential technique of discrete optimization for achieving the minimum cost of plane steel frames, taking into consideration the EC-3 specifications as well as including effects of the semi-rigid joints and non-prismatic elements in the design process. Likewise, an estimate of their influence on the final design is an aim of this work. The final objective is to draw practical conclusions which can be handful and easily applicable for a steel-structure project. An enormous amount of technical and scientific publications regarding steel frames is currently available, thus making the achievement of a comprehensive and updated knowledge a considerably hard task. In this work, a large variety of information has been gathered and classified, especially that related to current research lines and needs. Thus, the literature collected encompasses references related to state-of-the-art design methods, factors influencing the structural response, modelling and optimization techniques, as well as calculation and updated guidelines of some steel Design Codes about the subject. In this work a modelling procedure based on the finite element implemented within the MatLab programming environment has been performed. Several keys aspects have been included, such as second order behaviour, the safety assessment against structural instability and the search for an optimal cost considering the limit states according to EC-3 specifications. The flexibility of joints has been taken into account in the procedure hereby presented; its effects on the structural response, on the optimum weight and on the final cost have also been analysed. In order to confirm the validity and adequacy of this procedure, some application examples have been carried out. The results obtained were compared with those available from other authors. Several conclusions about the procedure that comprises modelling, analysis and design stages, as well as the effect of the flexibility of connections on the structural response have been drawn. The purpose is to point out some guidelines for the early stages of a project. One of the contributions of this thesis is an attempt for optimizing plane steel frames in which both non-prismatic beam-column-type elements and semi-rigid connections have been considered. Thus, an elastic stiffness matrix has been derived. Its validity has been tested through comparing its accuracy with other analytically-obtained matrices available in the literature, and with results obtained by the commercial software SAP2000. Another achievement of this work is the development of a method for cost optimization of plane steel building frames in which some relevant aspects have been taken in consideration. These encompass geometric imperfections, non-prismatic beam elements and the numerical characterization of semi-rigid connections, evaluating the effect of its flexibility on the structural response. Hence, some parametric analyses have been performed in order to assess the sensitivity, the stability of the outcomes and their range of applicability as well.

Idoneidad del grado de rigidez de las uniones en pórticos metálicos de naves a dos aguas en edificación agroindustrial

Para el análisis de la respuesta estructural, tradicionalmente se ha partido de la suposición de que los nudos son totalmente rígidos o articulados. Este criterio facilita en gran medida los cálculos, pero no deja de ser una idealización del comportamiento real de las uniones. Como es lógico, entre los nudos totalmente rígidos y los nudos articulados existe una gama infinita de valores de rigidez que podrían ser adoptados. A las uniones que presentan un valor distinto de los canónicos se les denomina en sentido general uniones semirrígidas. La consideración de esta rigidez intermedia complica considerablemente los cálculos estructurales, no obstante provoca cambios en el reparto de esfuerzos dentro de la estructura, así como en la configuración de las propias uniones, que en ciertas circunstancias pueden suponer una ventaja económica. Del planteamiento expuesto en el párrafo anterior, surgen dos cuestiones que serán el germen de la tesis. Estas son: ¿Qué ocurre si se aplica el concepto de uniones semirrígidas a las naves industriales? ¿Existen unos valores determinados de rigidez en sus nudos con los que se logra optimizar la estructura? Así, surge el objetivo principal de la tesis, que no es otro que conocer la influencia de la rigidez de los nudos en los costes de los pórticos a dos aguas de estructura metálica utilizados típicamente en edificios de uso agroindustrial. Para alcanzar el objetivo propuesto, se plantea una metodología de trabajo que básicamente consiste en el estudio de una muestra representativa de pórticos sometidos a tres estados de carga: bajo, medio y alto. Su rango de luces abarca desde los 8 a los 20 m y el de la altura de pilares desde los 3,5 a los 10 m. Además, se considera que sus uniones pueden adoptar valores intermedios de rigidez. De la combinatoria de las diferentes configuraciones posibles se obtienen 46.656 casos que serán objeto de estudio. Debido al fin economicista del trabajo, se ha prestado especial atención a la obtención de los costes de ejecución de las diferentes partidas que componen la estructura, incluidas las correspondientes a las uniones. Para acometer los cálculos estructurales ha sido imprescindible contar con un soporte informático, tanto existente como de creación ex profeso, que permitiese su automatización en un contexto de optimización. Los resultados del estudio consisten básicamente en una cantidad importante de datos que para su interpretación se hace imprescindible tratarlos previamente. Este tratamiento se fundamenta en su ordenación sistemática, en la aplicación de técnicas estadísticas y en la representación gráfica. Con esto se obtiene un catálogo de gráficos en los que se representa el coste total de la estructura según los diferentes valores de rigidez de sus nudos, unas matrices resumen de resultados y unos modelos matemáticos que representan la función coste total - rigideces de nudos. Como conclusiones se puede destacar: por un lado que los costes totales de los pórticos estudiados son mínimos cuando los valores de rigidez de sus uniones son bajos, concretamente de 5•10³ a 10•10³ kN•m/rad; y por otro que la utilización en estas estructuras de uniones semirrígidas con una combinación idónea de sus rigideces, supone una ventaja económica media del 18% con respecto a las dos tipologías que normalmente se usan en este tipo de edificaciones como son los pórticos biempotrados y los biarticulados. ABSTRACT Analyzing for structural response, traditionally it started from the assumption that joints are fully rigid or pinned. This criterion makes the design significantly easier, but it is also an idealization of real joint behaviour. As is to be expected, there is an almost endless range of stiffnes value between fully rigid and pinned joints, wich could be adopted. Joints with a value other than traditional are referred to generally as semi-rigid joints. If middle stiffness is considered, the structural design becomes much more complicated, however, it causes changes in the distribution of frame stresses, as well as on joints configuration, that under certain circumstances they may suppose an economic advantage. Two questions arise from the approach outlined in the preceding subparagraph, wich are the seeds of the doctoral thesis. These are: what happens when the concept of semirigid joints is applied to industrial buildings? There are certain stiffness values in their joints with which optimization of frame is achieved? This way, the main objective of the thesis arise, which is to know the influence of stiffness of joints in the cost of the steel frames with gabled roof, that they are typically used in industrial buildings. In order to achieve the proposed goal, a work methodology is proposed, which consists in essence in a study of a representative sample of frames under three load conditions: low, middle and high. Their range of spans comprises from 8 to 20 m and range of the height of columns cover from 3,5 to 10 m. Furthermore, it is considered that their joints can adopt intermediate values of stiffness. The result of the combination of different configurations options is 46.656 cases, which will be subject of study. Due to the economic aim of this work, a particular focus has been devoted for obtaining the execution cost of the different budget items that make up the structure, including those relating to joints. In order to do the structural calculations, count with a computing support has been indispensable, existing and created expressly for this purpose, which would allows its automation in a optimization context. The results of the study basically consist in a important amount of data, whose previous processing is necesary. This process is based on his systematic arrangement, in implementation of statistical techniques and in graphical representation. This give a catalogue of graphics, which depicts the whole cost of structure according to the different stiffness of its joints, a matrixes with the summary of results and a mathematical models which represent the function whole cost - stiffness of the joints. The most remarkable conclusions are: whole costs of the frames studied are minimum when the stiffness values of their joints are low, specifically 5•10³ a 10•10³ kN•m/rad; and the use of structures with semi-rigid joints and a suitable combination of their stiffness implyes an average economic advantage of 18% over other two typologies which are used typically in this kind of buildings; these are the rigid and bi-articulated frames.

Oxidation and tensile behavior of ferritic/martensitic steels after exposure to lead-bismuth eutectic

Two ferritic/martensitic steels, T91 steel and newly developed SIMP steel, were subject to tensile test after being oxidized in the liquid lead-bismuth eutectic (LBE) at 873 K for 500 h, 1000 h and 2000 h. Tensile tests were also carried out on the steels only thermally aged at 873 K. The result shows that thermal aging has no effect. Exposure to LBE at 873 K leads to a slight decrease in strength, but a large decrease in elongation when tested at 873 K. When tested at 873 K after 2000 h exposure, the tensile strength of T91 decreases slightly, and elongation from 39% to 21%. For SIMP, the decreases are slightly and from 44% to 28%, for tensile strength and elongation, respectively. The room temperature strength has slightly larger percentage reductions after the LBE exposure, but the elongation changes little.

Artificial Skin - Culturing of Different Skin Cell Lines for Generating an Artificial Skin Substitute on Cross-Weaved Spider Silk Fibres

Background: In the field of Plastic Reconstructive Surgery the development of new innovative matrices for skin repair is in urgent need. The ideal biomaterial should promote attachment, proliferation and growth of cells. Additionally, it should degrade in an appropriate time period without releasing harmful substances, but not exert a pathological immune response. Spider dragline silk from Nephila spp meets these demands to a large extent. Methodology/Principal Findings: Native spider dragline silk, harvested directly out of Nephila spp spiders, was woven on steel frames. Constructs were sterilized and seeded with fibroblasts. After two weeks of cultivating single fibroblasts, keratinocytes were added to generate a bilayered skin model, consisting of dermis and epidermis equivalents. For the next three weeks, constructs in co-culture were lifted on an originally designed setup for air/liquid interface cultivation. After the culturing period, constructs were embedded in paraffin with an especially developed program for spidersilk to avoid supercontraction. Paraffin cross-sections were stained in Haematoxylin & Eosin (H&E) for microscopic analyses. Conclusion/Significance: Native spider dragline silk woven on steel frames provides a suitable matrix for 3 dimensional skin cell culturing. Both fibroblasts and keratinocytes cell lines adhere to the spider silk fibres and proliferate. Guided by the spider silk fibres, they sprout into the meshes and reach confluence in at most one week. A well-balanced, bilayered cocultivation in two continuously separated strata can be achieved by serum reduction, changing the medium conditions and the cultivation period at the air/liquid interphase. Therefore spider silk appears to be a promising biomaterial for the enhancement of skin regeneration.

Bond strength of orthodontic brackets using different light and self-curing cements

The purpose of the study was to evaluate the shear bond strength of stainless steel orthodontic brackets directly bonded to extracted human premolar teeth. Fifty teeth were randomly divided into ¿ve groups: (1) System One (chemically cured composite resin), (2) Light Bond (light-cured composite resin), (3) Vivaglass Cem (self-curing glass ionomer cement), (4) Fuji Ortho LC (light-cured glass ionomer cement) used after 37% orthophosphoric acid¿etching of enamel (5) Fuji Ortho LC without orthophosphoric acid¿etching. The brackets were placed on the buccal and lingual surfaces of each tooth, and the specimens were stored in distilled water (24 hours) at 378C and thermocycled. Teeth were mounted on acrylic block frames, and brackets were debonded using an Instron machine. Shear bond strength values at fracture (Nw)were recorded. ANOVA and Student-Newman-Keuls multiple comparison tests were performed (P , .05). Bonding failure site was recorded by stereomicroscope and analyzed by Chi-square test, selected specimens of each group were observed by scanning electron microscope. System One attained the highest bond strength. Light Bond and Fuji Ortho LC, when using an acid-etching technique, obtained bond strengths that were within the range of estimated bond strength values for successful clinical bonding. Fuji Ortho LC and Vivaglass Cem left an almost clean enamel surface after debracketing.