System migration in ARL libraries /

"June 1992"

Arquitectura de sitios Web de bibliotecas universitarias : El sistema de bibliotecas de la Universidad Nacional de La Plata, Argentina

Este trabajo descriptivo exploratorio se propone analizar la arquitectura de información (AI) de sitios Web de bibliotecas de la Universidad Nacional de La Plata (UNLP), Argentina. Se analizaron 17 bibliotecas y se aplicó una grilla para recabar 10 aspectos relevantes. Los resultados fueron: 1. Ubicación del sitio Web de la biblioteca: 9 sitios incluidos en la página principal de la facultad. 2. Etiquetado de contenidos: terminología simple, sin jergas; no hay homogeneidad entre las bibliotecas. 3. Capacidad de búsqueda: 62 por ciento positiva, 38 por ciento negativa. 4. Sistema de búsqueda: simple 43 por ciento, compleja 10 por ciento, con ayudas 10 por ciento, ninguno 38 por ciento. 5. Sistemas de navegación: globales 5 por ciento, jerárquicos 79 por ciento, locales 5 por ciento, ninguno 11 por ciento. 6. Herramientas de navegación: barras 16 por ciento, frames o marcos 30 por ciento, índices 2 por ciento, mapas de sitio 7 por ciento, menús horizontales 9 por ciento, menús verticales 35 por ciento. 7. Sindicación de contenidos RSS: 3 sitios. 8. Otros servicios: chat 7 por ciento, descarga de documentos 16 por ciento, envío de formularios 14 por ciento, instructivos 21 por ciento, links a otras páginas 23 por ciento, tutoriales 5 por ciento, otros 14 por ciento. 9. Accesibilidad Web: 1 sitio. 10. Otras observaciones: ninguna. Se concluye que el desarrollo de los sitios es dispar y se recomienda considerar pautas de AI como parte de la cooperación en la red de bibliotecas de la UNLP

Arquitectura de sitios Web de bibliotecas universitarias : El sistema de bibliotecas de la Universidad Nacional de La Plata, Argentina

Este trabajo descriptivo exploratorio se propone analizar la arquitectura de información (AI) de sitios Web de bibliotecas de la Universidad Nacional de La Plata (UNLP), Argentina. Se analizaron 17 bibliotecas y se aplicó una grilla para recabar 10 aspectos relevantes. Los resultados fueron: 1. Ubicación del sitio Web de la biblioteca: 9 sitios incluidos en la página principal de la facultad. 2. Etiquetado de contenidos: terminología simple, sin jergas; no hay homogeneidad entre las bibliotecas. 3. Capacidad de búsqueda: 62 por ciento positiva, 38 por ciento negativa. 4. Sistema de búsqueda: simple 43 por ciento, compleja 10 por ciento, con ayudas 10 por ciento, ninguno 38 por ciento. 5. Sistemas de navegación: globales 5 por ciento, jerárquicos 79 por ciento, locales 5 por ciento, ninguno 11 por ciento. 6. Herramientas de navegación: barras 16 por ciento, frames o marcos 30 por ciento, índices 2 por ciento, mapas de sitio 7 por ciento, menús horizontales 9 por ciento, menús verticales 35 por ciento. 7. Sindicación de contenidos RSS: 3 sitios. 8. Otros servicios: chat 7 por ciento, descarga de documentos 16 por ciento, envío de formularios 14 por ciento, instructivos 21 por ciento, links a otras páginas 23 por ciento, tutoriales 5 por ciento, otros 14 por ciento. 9. Accesibilidad Web: 1 sitio. 10. Otras observaciones: ninguna. Se concluye que el desarrollo de los sitios es dispar y se recomienda considerar pautas de AI como parte de la cooperación en la red de bibliotecas de la UNLP

Arquitectura de sitios Web de bibliotecas universitarias : El sistema de bibliotecas de la Universidad Nacional de La Plata, Argentina

Este trabajo descriptivo exploratorio se propone analizar la arquitectura de información (AI) de sitios Web de bibliotecas de la Universidad Nacional de La Plata (UNLP), Argentina. Se analizaron 17 bibliotecas y se aplicó una grilla para recabar 10 aspectos relevantes. Los resultados fueron: 1. Ubicación del sitio Web de la biblioteca: 9 sitios incluidos en la página principal de la facultad. 2. Etiquetado de contenidos: terminología simple, sin jergas; no hay homogeneidad entre las bibliotecas. 3. Capacidad de búsqueda: 62 por ciento positiva, 38 por ciento negativa. 4. Sistema de búsqueda: simple 43 por ciento, compleja 10 por ciento, con ayudas 10 por ciento, ninguno 38 por ciento. 5. Sistemas de navegación: globales 5 por ciento, jerárquicos 79 por ciento, locales 5 por ciento, ninguno 11 por ciento. 6. Herramientas de navegación: barras 16 por ciento, frames o marcos 30 por ciento, índices 2 por ciento, mapas de sitio 7 por ciento, menús horizontales 9 por ciento, menús verticales 35 por ciento. 7. Sindicación de contenidos RSS: 3 sitios. 8. Otros servicios: chat 7 por ciento, descarga de documentos 16 por ciento, envío de formularios 14 por ciento, instructivos 21 por ciento, links a otras páginas 23 por ciento, tutoriales 5 por ciento, otros 14 por ciento. 9. Accesibilidad Web: 1 sitio. 10. Otras observaciones: ninguna. Se concluye que el desarrollo de los sitios es dispar y se recomienda considerar pautas de AI como parte de la cooperación en la red de bibliotecas de la UNLP

A percepção dos bibliotecários quanto ao sistema de administração de bibliotecas Argo: um estudo de caso na Universidade Federal do Rio Grande

O presente trabalho possuiu como tema um estudo a respeito da percepção dos bibliotecários do Sistema de Bibliotecas (SIB) da Universidade Federal do Rio Grande (FURG) quanto ao Sistema de Administração de Bibliotecas (ARGO). Convém dizer que na atualidade falar dos sistemas informatizados é buscar caminhos para um suporte melhor a informação e ampliar o acesso para os usuários. Assim, o objetivo geral deste trabalho foi investigar a percepção dos bibliotecários quanto ao sistema de administração de bibliotecas ARGO. Sendo os objetivos específicos identificar os bibliotecários que atuam junto ao sistema; conhecer a opinião dos pesquisados sobre o sistema utilizado; refletir, a luz da literatura sobre os resultados da pesquisa; e apresentar a direção do SIB os resultados, de modo a colaborar com o Sistema. Foi utilizado como metodologia uma pesquisa exploratória, quali-quantitativa e também um estudo de caso. Entre os principais resultados se destaca que o sistema não possui os atributos necessários para um bom funcionamento. Nas considerações se observou que durante um tempo o ARGO atendeu as necessidades da instituição, mas devido ao grande crescimento da universidade e do volume de trabalho, ele deixou a desejar.

Protein complementation assay as a display system for screening protein libraries in the intracellular environment

A wide range of screening strategies have been employed to isolate antibodies and other proteins with specific attributes, including binding affinity, specificity, stability and improved expression. However, there remains no high-throughput system to screen for target-binding proteins in a mammalian, intracellular environment. Such a system would allow binding reagents to be isolated against intracellular clinical targets such as cell signalling proteins associated with tumour formation (p53, ras, cyclin E), proteins associated with neurodegenerative disorders (huntingtin, betaamyloid precursor protein), and various proteins crucial to viral replication (e.g. HIV-1 proteins such as Tat, Rev and Vif-1), which are difficult to screen by phage, ribosome or cell-surface display. This study used the β-lactamase protein complementation assay (PCA) as the display and selection component of a system for screening a protein library in the cytoplasm of HEK 293T cells. The colicin E7 (ColE7) and Immunity protein 7 (Imm7) *Escherichia coli* proteins were used as model interaction partners for developing the system. These proteins drove effective β-lactamase complementation, resulting in a signal-to-noise ratio (9:1 – 13:1) comparable to that of other β-lactamase PCAs described in the literature. The model Imm7-ColE7 interaction was then used to validate protocols for library screening. Single positive cells that harboured the Imm7 and ColE7 binding partners were identified and isolated using flow cytometric cell sorting in combination with the fluorescent β-lactamase substrate, CCF2/AM. A single-cell PCR was then used to amplify the Imm7 coding sequence directly from each sorted cell. With the screening system validated, it was then used to screen a protein library based the Imm7 scaffold against a proof-of-principle target. The wild-type Imm7 sequence, as well as mutants with wild-type residues in the ColE7- binding loop were enriched from the library after a single round of selection, which is consistent with other eukaryotic screening systems such as yeast and mammalian cell-surface display. In summary, this thesis describes a new technology for screening protein libraries in a mammalian, intracellular environment. This system has the potential to complement existing screening technologies by allowing access to intracellular proteins and expanding the range of targets available to the pharmaceutical industry.

"Future fit your catalog" Panel [Presentation]:World Cat Local Implementation as part of a Consortia of Libraries within the University System of Maryland and Affiliated Institutitons

Power Point from Panel presentation giving implementation and search result displays and linking (17 slides)

Construction and characterization of two bacterial artificial chromosome libraries of Zhikong scallop, Chlamys farreri Jones et Preston, and identification of BAC clones containing the genes involved in its innate immune system

Large-insert bacterial artificial chromosome (BAC) libraries are necessary for advanced genetics and genomics research. To facilitate gene cloning and characterization, genome analysis, and physical mapping of scallop, two BAC libraries were constructed from nuclear DNA of Zhikong scallop, Chlamys farreri Jones et Preston. The libraries were constructed in the BamHI and MboI sites of the vector pECBAC1, respectively. The BamHI library consists of 73,728 clones, and approximately 99% of the clones contain scallop nuclear DNA inserts with an average size of 110 kb, covering 8.0x haploid genome equivalents. Similarly, the MboI library consists of 7680 clones, with an average insert of 145 kb and no insert-empty clones, thus providing a genome coverage of 1.1x. The combined libraries collectively contain a total of 81,408 BAC clones arrayed in 212 384-well microtiter plates, representing 9.1x haploid genome equivalents and having a probability of greater than 99% of discovering at least one positive clone with a single-copy sequence. High-density clone filters prepared from a subset of the two libraries were screened with nine pairs of Overgos designed from the cDNA or DNA sequences of six genes involved in the innate immune system of mollusks. Positive clones were identified for every gene, with an average of 5.3 BAC clones per gene probe. These results suggest that the two scallop BAC libraries provide useful tools for gene cloning, genome physical mapping, and large-scale sequencing in the species.

Modeling Information Diffusion in University Libraries: Assessing Peer Interaction Patterns as a Complex Dynamic System

This presentation was offered as part of the CUNY Library Assessment Conference, Reinventing Libraries: Reinventing Assessment, held at the City University of New York in June 2014.

Find it yourself! A brief course in the use of books and libraries under the contract system,

Mode of access: Internet.

Checking-in at the library : designing an ambient media system for social learning and collaboration opportunities

The knowledge economy of the 21st century requires skills such as creativity, critical thinking, problem solving, communication and collaboration (Partnership for 21st century skills, 2011) – skills that cannot easily be learnt from books, but rather through learning-by-doing and social interaction. Big ideas and disruptive innovation often result from collaboration between individuals from diverse backgrounds and areas of expertise. Public libraries, as facilitators of education and knowledge, have been actively seeking responses to such changing needs of the general public...

Learning beyond books : strategies for ambient media to improve libraries and collaboration spaces as interfaces for social learning

With the advent of digital media and online information resources, public libraries as physical destinations for information access are being increasingly challenged. As a response, many libraries follow the trend of removing bookshelves in order to provide more floorspace for social interaction and collaboration. Such spaces follow a Commons 2.0 model: they are designed to support collaborative work and social learning. The acquisition of skills and knowledge is facilitated as a result of being surrounded by and interacting with a community of likeminded others. Based on the results of a case study on a Commons 2.0 library space, this paper describes several issues of collaboration and social learning in public library settings. Acknowledging the significance of the architectural characteristics of the physical space, we discuss opportunities for ambient media to better reflect the social attributes of the library as a place; i.e. amplify the sense of other co-present library visitors and provide opportunities for shared encounters and conversations, which would remain invisible otherwise. We present the design of a user check-in system for improving the library as a physical destination for social learning, sharing, and inspiration for and by the community.

Rapid identification of cytochrome P450cam variants by in vivo screening of active site libraries

The selection of cytochrome P450 enzymes from large variant libraries, and the subsequent use of these enzymes in preparative scale biotransformations, remains a formidable challenge due to the complexities of the associated electron transport systems. Here, a powerful approach for the generation and screening of P450cam libraries for new function is presented that is both flexible and robust. A targeted library was generated wherein only the P450cam active-site amino acids Y96 and F98 were fully randomized and biotransformations, using a novel P450cam whole-cell system, were screened by GC–MS for the hydroxylation of diphenylmethane. One in 50 of the reactions screened, including 16 different variants, produced 4-hydroxydiphenylmethane with up to 92% conversion observed in the case of the Y96A variant. These results demonstrate a primary example of the screening of P450cam libraries in a format that is compatible with extension to preparative scale reactions.

Phagotopes derived by antibody screening of phage-displayed random peptide libraries vary in immunoreactivity: Studies using an exemplary monoclonal antibody, CII-C1, to type II collagen

Antibody screening of phage-displayed random peptide libraries to identify mimotopes of conformational epitopes is promising. However, because interpretations can be difficult, an exemplary system has been used in the present study to investigate whether variation in the peptide sequences of selected phagotopes corresponded with variation in immunoreactivity. The phagotopes, derived using a well-characterized monoclonal antibody, CII-C1, to a known conformational epitope on type II collagen, C1, were tested by direct and inhibition ELISA for reactivity with CII-C1. A multiple sequence alignment algorithm, PILEUP, was used to sort the peptides expressed by the phagotopes into clusters. A model was prepared of the C1 epitope on type II collagen. The 12 selected phagotopes reacted with CII-C1 by both direct ELISA (titres from < 100-11 200) and inhibition ELISA (20-100% inhibition); the reactivity varied according to the peptide sequence and assay format. The differences in reactivity between the phagotopes were mostly in accord with the alignment, by PILEUP, of the peptide sequences. The finding that the phagotopes functionally mimicked the C1 epitope on collagen was validated in that amino acids RRL at the amino terminal of many of the peptides were topographically demonstrable on the model of the C1 epitope. Notably, one phagotope that expressed the widely divergent peptide C-IAPKRHNSA-C also mimicked the C1 epitope, as judged by reactivity in each of the assays used: these included cross-inhibition of CII-C1 reactivity with each of the other phagotopes and inhibition by a synthetic peptide corresponding to that expressed by the most frequently selected phagotope, RRLPFGSQM. Thus, it has been demonstrated that multiple phage-displayed peptides can mimic the same epitope and that observed immunoreactivity of selected phagotopes with the selecting mAb can depend on the primary sequence of the expressed peptide and also on the assay format used.

Costs and Benefits of a Shared Digital Long-Term Preservation System

This paper describes the cost-benefit analysis of digital long-term preservation (LTP) that was carried out in the context of the Finnish National Digital Library Project (NDL) in 2010. The analysis was based on the assumption that as many as 200 archives, libraries, and museums will share an LTP system. The term ‘system’ shall be understood as encompassing not only information technology, but also human resources, organizational structures, policies and funding mechanisms. The cost analysis shows that an LTP system will incur, over the first 12 years, cumulative costs of €42 million, i.e. an average of €3.5 million per annum. Human resources and investments in information technology are the major cost factors. After the initial stages, the analysis predicts annual costs of circa €4 million. The analysis compared scenarios with and without a shared LTP system. The results indicate that a shared system will have remarkable benefits. At the development and implementation stages, a shared system shows an advantage of €30 million against the alternative scenario consisting of five independent LTP solutions. During the later stages, the advantage is estimated at €10 million per annum. The cumulative cost benefit over the first 12 years would amount to circa €100 million.