Analysis of integrated optofluidic lab-on-a-chip fluorescence biosensor based on transmittance of light through a fluidic gap

Sensitivity analysis is an important aspect to be looked into while designing lab-on-a-chip systems. In this paper we will be showing with appropriate design that the best sensitivity of the fluorescence biosensor is achieved for an optimal width of fluidic gap, corresponding to a particular mode spot size. We will be also showing that the sensitivity of the biosensor is affected by efficiency of light coupling, which is influenced by changes in the width of fluidic gap, refractive index of the fluid and higher order modes.

Sensitivity analysis based on mode mismatch for an optofluidic lab on a chip biosensor

In this paper we will be presenting the effect of fluidic gap, the effect of change of refractive index of the fluid contained in the gap, and the effect of higher order modes on the efficiency of light coupling and thus on the on the sensitivity of the sensor.

Sensitivity analysis based on Mode Mismatch for an optofluidic lab on a chip biosensor

In this paper we will be presenting the effect of fluidic gap, the effect of change of refractive index of the fluid contained in the gap, and the effect of higher order modes on the efficiency of light coupling and thus on the on the sensitivity of the sensor.

Analysis of integrated optofluidic lab-on-a-chip sensor based on refractive index and absorbance sensing

The analysis of a fully integrated optofluidic lab-on-a-chip sensor is presented in this paper. This device is comprised of collinear input and output waveguides that are separated by a microfluidic channel. When light is passed through the analyte contained in the fluidic gap, optical power loss occurs owing to absorption of light. Apart from absorption, a mode-mismatch between the input and output waveguides occurs when the light propagates through the fluidic gap. The degree of mode-mismatch and quantum of optical power loss due to absorption of light by the fluid form the basis of our analysis. This sensor can detect changes in refractive index and changes in concentration of species contained in the analyte. The sensitivity to detect minute changes depends on many parameters. The parameters that influence the sensitivity of the sensor are mode spot size, refractive index of the fluid, molar concentration of the species contained in the analyte, width of the fluidic gap, and waveguide geometry. By correlating various parameters, an optimal fluidic gap distance corresponding to a particular mode spot size that achieves the best sensitivity is determined both for refractive index and absorbance-based sensing.

Freestanding optical fibers fabricated in a glass chip by femtosecond laser micromachining for lab-on-a-chip application (vol 13, pg 7225, 2005)

An erratum is presented to correct the propagation loss of the freestanding optical fibers fabricated in glass chip. (c) 2006 Optical Society of America.

ZnO thin film surface acoustic wave based lab-on-a-chip

Lab-on-a-chip (LOC) is one of the most important microsystem applications with promise for use in microanalysis, drug development, diagnosis of illness and diseases etc. LOC typically consists of two main components: microfluidics and sensors. Integration of microfluidics and sensors on a single chip can greatly enhance the efficiency of biochemical reactions and the sensitivity of detection, increase the reaction/detection speed, and reduce the potential cross-contamination, fabrication time and cost etc. However, the mechanisms generally used for microfluidics and sensors are different, making the integration of the two main components complicated and increases the cost of the systems. A lab-on-a-chip system based on a single surface acoustic wave (SAW) actuation mechanism is proposed. SAW devices were fabricated on nanocrystalline ZnO thin films deposited on Si substrates using sputtering. Coupling of acoustic waves into a liquid induces acoustic streaming and motion of droplets. A streaming velocity up to ∼ 5cm/s and droplet pumping speeds of ∼lcm/s were obtained. It was also found that a higher order mode wave, the Sezawa wave is more effective in streaming and transportation of microdroplets. The ZnO SAW sensor has been used for prostate antigen/antibody biorecognition systems, demonstrated the feasibility of using a single actuation mechanism for lab-on-a-chip applications. © 2010 Materials Research Society.

ZnO based SAW and FBAR devices for lab-on-a chip applications

Acoustic wave devices were fabricated incorporating ZnO films deposited using both a standard rf magnetronand a novel High Target Utilisation (HiTUS) Sputtering System. Our results demonstrated the feasibility of using a single SAW-based actuation mechanism for both microfluidics and sensing. To further improve the sensitivity of our bio-sensors we have also investigated the use of Thin Film Bulk Acoustic Resonators.

GELIFICATION - A SIMPLE AND EFFICIENT METHOD FOR ON-CHIP STORAGE OF REAGENTS: TOWARDS LAB-ON-A-CHIP SYSTEMS FOR POINT-OF-CARE DIAGNOSTICS

In this study, we describe a simple and efficient method for on-chip storage of reagents for point-of-care (POC) diagnostics. The method is based on gelification of all reagents required for on-chip PCR-based diagnostics as a ready-to-use product. The result reported here is a key step towards the development of a ready and easy to use fully integrated Lab-on-a-chip (LOC) system for fast, cost-effective and efficient POC diagnostics analysis.

Design and simulation of an interdigital-chaotic advection micromixer for lab-on-a-chip applications

This paper presents the design and simulation of a novel passive micromixer. The micromixer consists of two inlet tanks, one mixing channel and two outlet channels. In order to maximise the mixing efficiency, the following considerations are made: (i) The inlet tanks are followed by a series of microchannels, in which the flow is split. The microchannels are arranged in an interdigital manner to maximise the contact area between the two flows. (ii) The microchannels attached to the lower inlet tank have an upward slope while those attached to the upper tank have a downward slope. The higher-density flow is fed to the lower inlet tank and gets an upward velocity before entering the mixing channel. (iii) Two triangular barriers are placed within the mixing channel to impose chaotic advection and perturb the less-mixed flow along the top and bottom surfaces of the channel. (iv) Finally, two outlet channels are incorporated to discard the less-mixed flow. Three-dimensional simulations are carried out to evaluate the performance of the micromixer. Simulations are performed in the absence and presence of the gravitational force to analyse the influence of gravity on the micromixer. Mixing efficiencies of greater than 92% are achieved using water and a 1011'density biological solvent as the mixing fluids.

Design and simulation of an interdigital-chaotic advection micromixer for lab-on-a-chip applications

The paper presents the design and simulation of a novel passive micromixer. The micromixer consists of two inlet tanks, one mixing channel and two outlet channels. In order to maximise the mixing efficiency, the following considerations are made : (i) The inlet tanks are followed by a series of microchannels, in which the flow is split. The microchannels are arranged in an interdigital manner to  maximise the contact area between the two flows. (ii) The microchannels attached to the lower inlet tank have an upward slope while those attached to the upper tank have a downward slope. The higher-density flow is fed to the lower inlet tank and gets an upward velocity before entering the mixing channel. (iii) Two triangular barriers are placed within the mixing channel to impose chaotic advection and perturb the less-mixed flow along the top and bottom surfaces of the channel. (iv) Finally, two outlet channels are incorporated to discard the less-mixed flow. Three-dimensional simulations are carried out to evaluate the performance of the micromixer. Simulations are performed in the absence and presence of the gravitational force to analyse the influence of gravity on the micromixer. Mixing efficiencies of greater than 92 % are achieved using water and a low-density biological solvent as the mixing fluids.

Non-optical biosensing in lab-on-a-chip systems

This paper presents a review of the recent trends and developments in non-optical biosensing platforms for lab-on- a-chip systems. This includes design considerations and applications of the non-optical biosensing platforms. The paper first categorizes the non-optical biosensors into four groups. The definition of each group together with a review of the reported works associated with the group are given. A performance analysis of different non-optical detection methods is also presented.