The experience of Thomas H. Jones, who was a slave for forty-three years. /

Consult: Dumond, D.L. Antislavery in America, page 70; Library Company of Philadelphia. Afro-Americana, 1553-1906, entry 5395.

The Oxytricha trifallax macronuclear genome: a complex eukaryotic genome with 16,000 tiny chromosomes.

The macronuclear genome of the ciliate Oxytricha trifallax displays an extreme and unique eukaryotic genome architecture with extensive genomic variation. During sexual genome development, the expressed, somatic macronuclear genome is whittled down to the genic portion of a small fraction (∼5%) of its precursor "silent" germline micronuclear genome by a process of "unscrambling" and fragmentation. The tiny macronuclear "nanochromosomes" typically encode single, protein-coding genes (a small portion, 10%, encode 2-8 genes), have minimal noncoding regions, and are differentially amplified to an average of ∼2,000 copies. We report the high-quality genome assembly of ∼16,000 complete nanochromosomes (∼50 Mb haploid genome size) that vary from 469 bp to 66 kb long (mean ∼3.2 kb) and encode ∼18,500 genes. Alternative DNA fragmentation processes ∼10% of the nanochromosomes into multiple isoforms that usually encode complete genes. Nucleotide diversity in the macronucleus is very high (SNP heterozygosity is ∼4.0%), suggesting that Oxytricha trifallax may have one of the largest known effective population sizes of eukaryotes. Comparison to other ciliates with nonscrambled genomes and long macronuclear chromosomes (on the order of 100 kb) suggests several candidate proteins that could be involved in genome rearrangement, including domesticated MULE and IS1595-like DDE transposases. The assembly of the highly fragmented Oxytricha macronuclear genome is the first completed genome with such an unusual architecture. This genome sequence provides tantalizing glimpses into novel molecular biology and evolution. For example, Oxytricha maintains tens of millions of telomeres per cell and has also evolved an intriguing expansion of telomere end-binding proteins. In conjunction with the micronuclear genome in progress, the O. trifallax macronuclear genome will provide an invaluable resource for investigating programmed genome rearrangements, complementing studies of rearrangements arising during evolution and disease.

Quelle, Brunnen und Zisterne im alten Testament

Leipzig, Phil. Diss., 1928

A Pillar of the Exchange

Karikierende Darstellung von N. M. Rothschild

Effects of genetic diversity of grass on insect species diversity at higher trophic levels are not due to cascading diversity effects

Genetic diversity in plant populations has been shown to affect the species diversity of insects. In grasses, infection with fungal endophytes can also have strong effects on insects, potentially modifying the effects of plant genetic diversity. We manipulated the genetic diversity and endophyte infection of a grass in a field experiment. We show that diversity of primary parasitoids (3rd trophic level) and, especially, secondary parasitoids (4th trophic level) increases with grass genetic diversity while there was no effect of endophyte infection. The increase in insect diversity appeared to be due to a complementarity effect rather than a sampling effect. The higher parasitoid diversity could not be explained by a cascading diversity effect because herbivore diversity was not affected and the same herbivore species were present in all treatments. The effects on the higher trophic levels must therefore be due to a direct response to plant traits or mediated by effects on traits at intermediate trophic levels.

On the power, wisdom, and goodness of God : as manifested in the creation of animals, and in their history, habits, and instincts /

Includes bibliographical references and index.

Traité pratique des maladies des yeux /

Mode of access: Internet.

Tratado práctico de las enfermedades de los ojos /

Mode of access: Internet.

Tratado práctico de las enfermedades de los ojos /

Mode of access: Internet.

Les reports de divers special cases en le Common bank & en le Court del bank le roy. En le reigne de le roy Charles le II. [1667-1684]

Signatures: [A]-Z⁴, Aa-Kk⁴, Ll-Oo².

Six sermons delivered at the general convention of Universalists, at its annual session in Concord, N.H., on the nineteenth and twentieth September, 1832.

Mode of access: Internet.

Conversations on natural philosophy : in which the elements of that science are familiarly explained /

Includes index.

Reports of cases argued and determined in the Court of Exchequer, in Ireland, from Michaelmas term, 5 Will. IV. to [Trinity term, 1 Victoria] both inclusive [1834-1838]

Includes index.