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Kirjallisuusarvostelu

Katarina von Braganza [Illustration] : (Porträt)

Samuel Cooper

LPF Rocek, Katarina.1; Rocek, Katarina

Anarchist, St. Louis. 1906. Portrait taken in Detroit

The protease inhibitor cystatin C is differentially expressed among dendritic cell populations, but does not control antigen presentation

Dendritic cells (DC) undergo complex developmental changes during maturation. The MHC class H (MHC H) molecules of immature DC accumulate in intracellular compartments, but are expressed at high levels on the plasma membrane upon DC maturation. It has been proposed that the cysteine protease inhibitor cystatin C (CyC) plays a pivotal role in the control of this process by regulating the activity of cathepsin S, a protease involved in removal of the MHC H chaperone E, and hence in the formation of MHC H-peptide complexes. We show that CyC is differentially expressed by mouse DC populations. CD8(+) DC, but not CD4(+) or CD4(-)CD8(-) DC, synthesize CyC, which accumulates in MHC II(+)Lamp(+) compartments. However, II processing and MHC H peptide loading proceeded similarly in all three DC populations. We then analyzed MHC H localization and Ag presentation in CD8(+) DC, bone marrow-derived DC, and spleen-derived DC lines, from CyC-deficient mice. The absence of CyC did not affect the expression, the subcellular distribution, or the formation of peptide-loaded MHC II complexes in any of these DC types, nor the efficiency of presentation of exogenous Ags. Therefore, CyC is neither necessary nor sufficient to control MHC II expression and Ag presentation in DC. Our results also show that CyC expression can differ markedly between closely related cell types, suggesting the existence of hitherto unrecognized mechanisms of control of CyC expression.

Projeto vida

Trata-se de um projeto de recep????o e atendimento ao p??blico, elaborado a partir da necessidade de humanizar e dinamizar o atendimento m??dico no Posto de Atendimento M??dico do M??ier (PAM-M??IER - Unidade de urg??ncia). O Projeto Vida, desenvolvido por uma equipe multiprofissional, em um ano de atendimento conseguiu: reduzir as filas de espera em 31,1 %, ou seja, um ter??o da clientela foi atendida sem demora nem burocracia; dar prioridade a casos graves e resolver de forma r??pida os casos simples e/ou que n??o necessitem de consulta m??dica. O projeto em pauta, foi o caminho alternativo para solucionar os problemas decorrentes do aumento progressivo da clientela e do d??ficit de recursos humanos em uma unidade de sa??de emergencial