Clinical staging versus laparotomy and combined modality with MOPP versus ABVD in early-stage hodgkin's disease: the H6 twin randomized trials from the european organization for research and treatment of cancer lymphoma cooperative group

info:eu-repo/semantics/published

Enteropathogenic Escherichia coli O125:H6 triggers attaching and effacing lesions on human intestinal biopsy specimens independently of Nck and TccP/TccP2

Typical enteropathogenic Escherichia coli (EPEC) and enterohemorrhagic E. coli (EHEC) employ either Nck, TccP/TccP2, or Nck and TccP/TccP2 pathways to activate the neuronal Wiskott-Aldrich syndrome protein (N-WASP) and to trigger actin polymerization in cultured cells. This phenotype is used as a marker for the pathogenic potential of EPEC and EHEC strains. In this paper we report that EPEC O125:H6, which represents a large category of strains, lacks the ability to utilize either Nck or TccP/TccP2 and hence triggers actin polymerization in vitro only inefficiently. However, we show that infection of human intestinal biopsies with EPEC O125:H6 results in formation of typical attaching and effacing lesions. Expression of TccP in EPEC O125:H6, which harbors an EHEC O157-like Tir, resulted in efficient actin polymerization in vitro and enhanced colonization of human intestinal in vitro organ cultures with detectable N-WASP and electron-dense material at the site of bacterial adhesion. These results show the existence of a natural category of EPEC that colonizes the gut mucosa using Nck- and TccP-independent mechanisms. Importantly, the results highlight yet again the fact that conclusions made on the basis of in vitro cell culture models cannot be extrapolated wholesale to infection of mucosal surfaces and that the ability to induce actin polymerization on cultured cells should not be used as a definitive marker for EPEC and EHEC virulence.

The problem of liquid droplet combustion-reexamination

The simple quasi-steady analysis of the combustion of a liquid fuel droplet in an oxidising atmosphere provides unsatisfactory explanations for several experimental observations. It's prediction of values for the burning constant (K), the flame-to-droplet diameter ratio ( ) and the flame temperature (Tf) have been found to be amgibuous if not completely inaccurate. A critical survey of the literature has led us to a detailed examination of the effects of unsteadiness and variable properties. The work published to date indicates that the gas-phase unsteadiness is relatively short and therefore quite insignificant.A new theoretical analysis based on heat transfer within the droplet is presented here. It shows that the condensed-phase unsteadiness lasts for about 20â??25% of the total burning time. It is concluded that the discrepancies between experimental observations and the predictions of the constant-property quasi-steady analysis cannot be attributed either to gas-phase or condensed-phase unsteadiness.An analytical model of quasi-steady droplet combustion with variable thermodynamic and transport properties and non-unity Lewis numbers will be examined. Further findings reveal a significant improvement in the prediction of combustion parameters, particularly of K, when consideration is given to variations of cp and λ with the temperature and concentrations of several species. Tf is accurately predicted when the required conditions of incomplete combustion or low ( ) at the flame are met. Further refinement through realistic Lewis numbers predicts ( ) meaningfully.

Anticancer Activity of Hydrogen-Bond-Stabilized Half-Sandwich RuII Complexes with Heterocycles

Neutral half-sandwich organometallic ruthenium(II) complexes of the type (?6-cymene)RuCl2(L)] (H1H10), where L represents a heterocyclic ligand, have been synthesized and characterized spectroscopically. The structures of five complexes were also established by single-crystal X-ray diffraction confirming a piano-stool geometry with ?6 coordination of the arene ligand. Hydrogen bonding between the N?H group of the heterocycle and a chlorine atom attached to Ru stabilizes the metalligand interaction. Complexes coordinated to a mercaptobenzothiazole framework (H1) or mercaptobenzoxazole (H6) showed high cytotoxicity against several cancer cells but not against normal cells. In vitro studies have shown that the inhibition of cancer cell growth involves primarily G1-phase arrest as well as the generation of reactive oxygen species (ROS). The complexes are found to bind DNA in a non-intercalative fashion and cause unwinding of plasmid DNA in a cell-free medium. Surprisingly, the cytotoxic complexes H1 and H6 differ in their interaction with DNA, as observed by biophysical studies, they either cause a biphasic melting of the DNA or the inhibition of topoisomerase IIa activity, respectively. Substitution of the aromatic ring of the heterocycle or adding a second hydrogen-bond donor on the heterocycle reduces the cytotoxicity.

Substitution-Modulated Anticancer Activity of Half-Sandwich Ruthenium(II) Complexes with Heterocyclic Ancillary Ligands

Ten new organometallic half-sandwich ruthenium complexes with heterocyclic ligands have been synthesized (H1-H10). The substituents on the ancillary heterocyclic ligands were varied to understand the effect of substitution on anticancer activity. The crystallographic characterization of five complexes confirms that they adopt three-legged piano-stool structures and are stabilized by intramolecular hydrogen bonding. Complexes H2 and H3 also exhibit halogen bonding in the solid state. In aqueous media, the complexes form dinuclear ruthenium species. Complex H1 with a noncytotoxic heterocycle, 6-fluoro-2-mercaptobenzothiazole, and complex H11 with the unsubstituted 2-mercaptobenzothiazole are the most active against A2780 and KB cell lines. The substitution of the H atoms on the ancillary ligand with Cl or Br atoms leads to a decrease in the anticancer activity. With the exception of fluorine-substituted H5, the complexes with mercaptobenzoxazole (H6-H9) are inactive against all of the tested cell lines. Ruthenium complexes with mercaptonaphthimidazole (H10) and mercaptobenzimidazole (H13) do not show any anticancer activity. The active complexes show a biphasic melting curve when incubated with calf thymus (CT) DNA. These complexes only inhibit thioredoxin reductase (TrxR) enzyme activity to a small extent. The substitution of hydrogen atoms with fluorine atoms in the aromatic heterocyclic ligands on organometallic half-sandwich ruthenium complexes has the most beneficial effect on their anticancer activity.

Interferon-gamma induced cell death: Regulation and contributions of nitric oxide, cJun N-terminal kinase, reactive oxygen species and peroxynitrite

Interferon-gamma (Ifn gamma), a known immunomodulatory cytokine, regulates cell proliferation and survival. In this study, the mechanisms leading to the selective susceptibility of some tumor cells to Ifn gamma were deciphered. Seven different mouse tumor cell lines tested demonstrated upregulation of MHC class I to variable extents with Ifn gamma; however, only the cell lines, H6 hepatoma and L929 fibrosarcoma, that produce higher amounts of nitric oxide (NO) and reactive oxygen species (ROS) are sensitive to Ifn gamma-induced cell death. NO inhibitors greatly reduce Ifn gamma-induced ROS; however, ROS inhibitors did not affect the levels of Ifn gamma-induced NO, demonstrating that NO regulates ROS. Consequently, NO inhibitors are more effective, compared to ROS inhibitors, in reducing Ifn gamma-induced cell death. Further analysis revealed that Ifn gamma induces peroxynitrite and 3-nitrotyrosine amounts and a peroxynitrite scavenger, FeTPPS, reduces cell death. Ifn gamma treatment induces the phosphorylation of c-jun N-terminal kinase (Jnk) in H6 and L929 but not CT26, a colon carcinoma cell line, which is resistant to Ifn gamma-mediated death. Jnk activation downstream to NO leads to induction of ROS, peroxynitrite and cell death in response to Ifn gamma. Importantly, three cell lines tested, i.e. CT26, EL4 and Neuro2a, that are resistant to cell death with Ifn gamma alone become sensitive to the combination of Ifn gamma and NO donor or ROS inducer in a peroxynitrite-dependent manner. Overall, this study delineates the key roles of NO as the initiator and Jnk, ROS, and peroxynitrite as the effectors during Ifn gamma-mediated cell death. The implications of these findings in the Ifn gamma-mediated treatment of malignancies are discussed. (C) 2014 Elsevier B.V. All rights reserved.

Interferon-gamma induced cell death: Regulation and contributions of nitric oxide, cJun N-terminal kinase, reactive oxygen species and peroxynitrite

Interferon-gamma (Ifn gamma), a known immunomodulatory cytokine, regulates cell proliferation and survival. In this study, the mechanisms leading to the selective susceptibility of some tumor cells to Ifn gamma were deciphered. Seven different mouse tumor cell lines tested demonstrated upregulation of MHC class I to variable extents with Ifn gamma; however, only the cell lines, H6 hepatoma and L929 fibrosarcoma, that produce higher amounts of nitric oxide (NO) and reactive oxygen species (ROS) are sensitive to Ifn gamma-induced cell death. NO inhibitors greatly reduce Ifn gamma-induced ROS; however, ROS inhibitors did not affect the levels of Ifn gamma-induced NO, demonstrating that NO regulates ROS. Consequently, NO inhibitors are more effective, compared to ROS inhibitors, in reducing Ifn gamma-induced cell death. Further analysis revealed that Ifn gamma induces peroxynitrite and 3-nitrotyrosine amounts and a peroxynitrite scavenger, FeTPPS, reduces cell death. Ifn gamma treatment induces the phosphorylation of c-jun N-terminal kinase (Jnk) in H6 and L929 but not CT26, a colon carcinoma cell line, which is resistant to Ifn gamma-mediated death. Jnk activation downstream to NO leads to induction of ROS, peroxynitrite and cell death in response to Ifn gamma. Importantly, three cell lines tested, i.e. CT26, EL4 and Neuro2a, that are resistant to cell death with Ifn gamma alone become sensitive to the combination of Ifn gamma and NO donor or ROS inducer in a peroxynitrite-dependent manner. Overall, this study delineates the key roles of NO as the initiator and Jnk, ROS, and peroxynitrite as the effectors during Ifn gamma-mediated cell death. The implications of these findings in the Ifn gamma-mediated treatment of malignancies are discussed. (C) 2014 Elsevier B.V. All rights reserved.

云南18 个民族Y染色体双等位基因单倍型频率的主成分分析

世居云南的少数民族中,壮、傣、水、布依、布朗、德昂、佤、彝、白、怒、哈尼、傈僳、拉祜、纳西、景颇、阿昌、基 诺和独龙18 个民族是由“羌”、“濮”、“越”3 大部落群体演化而来,是云南的土著居民。利用PCR2RFL P 方法对这18 个土著民族进行Y染色体上13 个双等位基因位点进行基因分型。结果显示,不同历史族源的民族群体在Y染 色体双等位基因单倍型分布上具有一定的差异:在百越后裔民族群体中以单倍型H11 、H12 为主要分布;在氐羌后 裔民族中以单倍型H5 、H6 和H8 为主要分布;在百濮后裔民族群体中主要单倍型分布为H6 、H8 和H11 。进一步 主成分分析表明,百越后裔民族群体和氐羌后裔民族在主成分图上聚为两组,提示父系基因库有不同的来源,与历 史记载相印证。

A taxonomic study of some red algae commonly growing on the coast of Karachi

Eighteen commonly occurring species of marine benthic red algae, i.e., Asparagopsis taxiformis (Delile) Trevisan, Bangia atropurpurea (Roth) C. Agardh, Centroceras clavulatum (C. Agardh) Montagne, Calliblepharis fimbriata (Greville) Kiitzing, Coelarthrum muelleri (Sonder) B¢rgesen, Cottoniella filamentosa (Howe) B¢rgesen, Gracilariafoliifera (Forsskai) B¢rgesen, Halymenia porphyraeformis (B¢rgesen) Parkinson, Hypnea muscifor:mis (Wulfen) Lamouroux, Hypnea valentiae (Turner) Montagne, Laurencia obtusa (Hudson) Lamouroux, Me!anothamnus somalensis Bomet et Falkenberg, Porphyra vietnamensis Tanaka et Pham-hoang H6, Sarconema filiforme (Sonder) Kylin, Sebdenia flabellata (J. Agardh) Parkinson, Scinaia fascicularis (B¢rgesen) Huisman, Scinaia hatei B¢rgesen, and Solieria robusta (Greville) Kylin were collected from coastal areas near Karachi (Pakistan) and taxonomically investigated. All the investigated seaweeds are taxonomically known species. During this study, Melanothamnus somalensis is reported for the first time from northern Arabian Sea and Asparagopsis taxiformis, Bangia atropurpurea, Cottoniella filamentosa, Gracilaria foliifera, Halymenia porphyraeformis, Melanothamnus somalensis, Sarconema filiforme, Sebdenia flabellata, Scinaia fascicularis, and Solieria robusta are taxonomically described for the first time from the coast of Pakistan.

番茄灰霉病菌拮抗菌D2-4发酵条件的研究

对拮抗番茄灰霉病菌的 D2 - 4菌株培养基成分和发酵条件进行研究。结果表明 ,在 2 8℃ ,180 r· min- 1 振荡培养条件下 ,最佳发酵时间为 96 h左右 ,培养 2 0～ 2 4 h的种子液以 7%的接种量转接有利于提高抑菌活性 ,装液量为6 0 m L· 2 5 0 m L- 1 三角瓶 ,均匀设计试验得出 ,最佳培养基配方为 (10 0 m L发酵培养基中含 ) :黄豆饼粉 1.10 g、葡萄糖 2 .71g、蔗糖 1.0 0 g、Na Cl0 .10 g、酵母膏 0 .10 g、p H6 .6 1。

La(1-x)Pr_xP_5O_(14)晶体的研究

用水热法研究了La12xPr xP5O14(LaPrPP)(0 < x < 1,原子百分含量)晶体的生长,测定了La0.2Pr0.8PP晶体结构为 单斜晶系,空间群为P21/c。晶体的密度为3.27g/cm3,分解温度为915℃。分析了室温下晶体在紫外、可见区的吸 收、荧光和红外光谱,利用Judd2Ofeld 理论计算了PrPP 和La0. 2Pr0. 8PP 晶体中Pr3 + 的实验振子强度,得到一些规律性结果,观察到Pr3 + 的3 P0 —3 H6 跃迁的红色发射很强。

环戊二烯—12-钼硅杂多四丁基铵电荷转移盐的合成、表征与晶体结构

以环戊二烯与 1 2 钼硅杂多四丁基铵为原料 ,采用光化学法合成了一种新型电荷转移盐 (Bu4N) 4(C5 H6 ) [HSiMoVI11MoVO40 ]。用元素分析、IR、CV、固体漫反射电子光谱、ESR进行了表征。X 射线晶体结构测定其晶体属三斜晶系 ,空间群P1 ,晶胞参数a =1 4.347(3) ,b =1 4.42 3(3) ,c =2 7.1 5 8(5 ) ,α =96 .90 (3) ,β =1 0 4.1 8(3) ,γ =98.2 0 (3)° ,V =5 32 2 (2 ) 3,Z =2 ,Mr=2 85 5 .30 ,Dc=1 .782 g·cm-3,F(0 0 0 ) =2 86 0 ,R =0 .0 71 9,wR =0 .1 983。标题化合物由 1个环戊二烯、4个Bu4N+阳离子和 1个 [SiMoVI11MoVO40 ]4 -阴离子构成。

南海沉积物中的放射虫及其古环境意义

In this paper a systematic study of radiolarian from surface sediments of all the South China Sea (SCS) has initially been done for its deposit ecology, biogeography and paleoenvironment significance. The paleoenvironment information obtained by radiolarian analysis and other sedimentary method for core samples is also made use as the synthesis proxy for revealing the paleoenvironment changes in the SCS and the relations of it with the past globe change during last 200ka. Some results come out of this study as: 1) Radiolarian skeleton chemistry composition and skeleton morphological features were analyzed, chiefly dividing them as 15 types of basic morphological features: 2) Analysis of biogeographical feature demonstrates that the fauna in SCS obviously belonging to a transitional type of west Pacific - Indian Ocean and has a particularity of itself: 3) Tendency of radiolarian population distribution is lower in shallow continental shelf area, increasing gradually toward the abyssal region; 4) Nine character boundaries of radiolarian depth distribution in the sediments from whole the South China Sea may be recognized; 5)Two radiolarian transfer functions for paleotemperature and paleo-primary productivity in the South China Sea have also been established respectively. The equation of transfer function for winter paleotemperature has only an average estimated error of 0.18678 ℃ and the equation for paleo-primary productivity has the calculation accuracy of 85.31%; 6) Changes of radiolarian individuals, numbers of species and H(S) values in core NS93-5 show the completely different oceanic geographical circumstance and ecology structure in the Last Maximum Glacial with present; 7) The abundance variation of some raiolarian warm species and cold species indicate the changes of water masse features along with the paleoenvironment evolution, showing that this sea area clearly is controlled in 6 issues of oxygen isotopes by the cold water masses; 8) By comparative analysis of δ ~(18)O curves with GISP2;s ice core can core 17940 of the northern SCS, the occurring characters of D/O's events 1-21 and Heinrich's events H1-H6 have been revealed in this sea area by core NS93-5, which prove the existence of paleoclimatic tele-connections between the southern SCS and Arctic region since about 200ka BP.