Creatine supplementation and oxidative stress in rat liver

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Redox dysregulation and bioaccumulation of copper in tilapia Oreocromis niloticus

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Evaluation of the Antihypertensive Properties of Yellow Passion Fruit Pulp (Passiflora edulis Sims f. flavicarpa Deg.) in Spontaneously Hypertensive Rats

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Associação entre a concentração de selênio, o polimorfismo Pro198Leu da glutationa peroxidase 1 e a mortalidade em pacientes com choque séptico

Pós-graduação em Fisiopatologia em Clínica Médica - FMB

Mecanismos da infertilidade causada por Gossipol em ratos e efeito protetor da vitamina E

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Cytotoxic, biochemical and genotoxic effects of biodiesel produced by different routes on ZFL cell line

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Oxidative Stress in Maternal Blood and Placenta From Mild Diabetic Rats

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Erythrocyte selenium concentration predicts intensive care unit and hospital mortality in patients with septic shock: a prospective observational study

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cardiac Energy Metabolism and Oxidative Stress Biomarkers in Diabetic Rat Treated with Resveratrol

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Improvement of Pro-Oxidant Capacity of Protocatechuic Acid by Esterification

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

FLUXOFENIM USED AS A SAFENER ON SORGHUM SEED FOR S-METOLACHLOR HERBICIDE

This study aimed to evaluate the efficiency of protective fluxofenim seed treatment of sorghum hybrids DKB510 and SCG340, in order to increase the selectivity to the herbicide S-metolachlor applied pre-emergence and to determine the activity of detoxification enzyme glutathione S-transferase (GST). This work was divided into two stages. The first step consisted of field evaluation of the effectiveness of the shield to reduce visual symptoms of plant injury caused by the herbicide and the second determined the activity of GST. It was compared the susceptibility of sorghum seeds to the herbicide by means of visual assessment of injuries at 3, 7, 15 and 30 days after emergence (DAE), root dry weight and shoot at 10 DAE, and determination of activity GST. The treatments were: application of the safener dose at 0 and 40 mL per 100 kg of seed, and spraying of the herbicide S-metolachlor at rates of 1,440 and 2,880 g a.i. ha(-1), and a control without herbicide. The safener use to seed treatment for both sorghum hybrids (DKB510 and SCG340) increased tolerance to the herbicide S-metolachlor in two doses, and the best results were obtained at a dose of 1,440 g ha(-1). The GST enzyme activity showed an increase when using the fluxofenim prior to application of the herbicide S-metolachlor at a dose of 1,440 g ha(-1) for the two hybrids.

Avaliação da influência do estresse em modelo experimental de colite por administração de Ácido Trinitrobenzenosulfônico (TNBS) em concentração subinflamatória

Inflammatory bowel disease (IBD) is a chronic and relapsing disease caused by exaggerated response of the immune system. It represents a significant health problem by limiting the quality of life and being the main risk factor for colorectal cancer. Despite of its importance, the high worldwide incidence and being the object of research for several decades, the etiology remains unknown. Studies indicates an interaction between genetic and environmental factors which together with the intestinal microbiota, leads to an uncontrolled immune response. One of the aggravating environmental factors often discussed is stress, as the daily life of the population in general is increasingly rushed. In order to demonstrate the influence of stress on IBD, this study aimed to standardize an experimental model of colitis induced by instillation of a trinitrobenzene sulfonic acid (TNBS) noninflammatory concentration plus exposure to stress that intensify the inflammation. Therefore, an experiment was done to determine what would be the noninflammatory concentration. In this step, four different concentrations of TNBS (1, 6, 12.5 or 40mg/ml) were tested and the lowest concentration capable of inducing a noninflammatory response in the gut was defined as 1 mg/ml. Then, a second experiment was performed which induced colitis and exposed the animals to restraint stress. The results, however, showed that this stimulus was not enough to exacerbate the damage caused by the 1 mg/ml concentration of TNBS in the colon. With some changes in the protocol, the third experiment associated cold and restraint, as well as changes on the day of euthanasia, which occurred immediately after the stress session. The results of myeloperoxidase activity measurement were unexpected due to the noninflammatory concentration of TNBS caused an intestinal inflammation similar to the concentration of 40 mg/ml. However, the results of glutathione quantification and the corticosterone ...

Estudo da oxidação da melatonina e de seus produtos por radicais peroxila

Melatonin is an endoleamine that has anti-inflammatory, immunomodulating and antioxidants properties. But there is a contradiction between the antioxidant effects of melatonin and glutathione (GSH). Therefore, the main objective of this work was to study the effect of melatonin on the oxidation of GSH and the effect of GSH on the oxidation of melatonin by peroxyl radicals generated by thermolysis of 2,2 -Azobis(2- amino-propane)-dihydrochloride (AAPH). The influence of the reaction conditions and the identity of the products of oxidation were also studied. The main products obtained during the oxidation of melatonin were its monohydroxylated derivative and N1-acetyl- N2-formyl-5-methoxykynuramine (AFMK), which is the product obtained by oxidative cleavage of the melatonin indole ring. By studying the buffer type, pH and the presence or absence of dissolved oxygen in the reaction system, it was observed that, the yield of AFMK was higher when the pH or the concentration of oxygen was increased. Comparing the reactivity of both molecules GSH and melatonin, it can be seen that intermediates radicals generated during the oxidation of melatonin are able to oxidize GSH itself. We propose that this chemical property could justify the recent reports that demonstrated the inability of melatonin to inhibit the oxidation of GSH in cells

Avaliação gastroprotetora da epicatequina e verificação de seus mecanismos de ação

The stomach is an exceptional organ, which functions are sterilize food ingested, form the primitive bolus, digest lipids and proteins, and to store food temporarily in the gastrointestinal tract. Its capacity of digesting food without digesting itself is amazing. This fact occurs due to innumerous protective substances adjacent to the gastric mucosa. When aggressive factors overwhelm the protective factors, a lesion in the gastric mucosa is formed. Lesions that reach the lamina propria are called gastric ulcers, which are classified macroscopically as openings on the gastric wall and; microscopically, as a gastric injury characterized with epithelial desquamation, mucosal hemorrhage, glandular damage and eosinophilic infiltration. The current therapy available is effective, although it causes collateral effects, therefore researching new drugs is necessary. This work aim to evaluate the gastroprotective effect of epicatechin against gastric lesions induced by absolute ethanol and non steroidal anti-inflammatory drugs which are the main causes of this disease currently, yet we aim to study the main mechanisms of action responsible for the gastroprotective effect. The results show that epicatechin has a significant macroscopic and microscopic gastroprotective effect against gastric injuries induced by ethanol and indomethacin, acting locally by augmenting gastric mucus secretion and it also acts via antioxidant system by holding total glutathione levels. Epicatechin’s gastroprotective mechanisms depend on the activation of sulfhydryl compounds and doesn’t depend on the NO-synthase enzyme

Estresse oxidativo na leishmaniose visceral canina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)