Screening marine algae from China for their antitumor activities

Thirty-nine species of marine algae collected from the coast of China were screened for their antitumor activities, and eight species Leathesia difformes, Polysiphonia urcedata, Scytosiphon lomentarius, Gloiopeliis furcata, Punctaria latifolia, Symphyocladia latiuscula, Rhodomela confervoides and Ulva pertusa showed potent cytotoxic activities. Three, Rhodomela confervoides, Scytosiphon lomentarius and Gloiopeliis furcata, were used for further investigation. More than 30 compounds were isolated and purified, and 14 bromophenols, 1 steroid and 1 carotene were identified by advanced spectroscopic methods including IR, MS, and NMR techniques. Amongst the 16 identified compounds, 7 showed vigorously selective activities against KB, Bel7402 and A549 cancer cells, and 6 bromophenols were new compounds.

Primary production of Utricularia foliosa L., Egeria densa Planchon and Cabomba furcata Schult & Schult.f from rivers of the coastal plain of the State of São Paulo, Brazil

Seasonal variation in gross primary production (GPP) of Utricularia foliosa Linnaeus, Egeria densa Planchon and Cabomba furcata Schult & Schult.f. in rivers of the coastal plain of the state of São Paulo, Brazil was examined in relation to water physico-chemistry. These three species do not affect the multiple uses of the streams and are present throughout the year. The most productive was U. foliosa (maximum production 24.7 mgO(2) g(-1) DW h(-1)), while C. furcata had an intermediate GPP (maximum production 17.5 mgO(2) g(-1) DW h(-1)) and E. densa was lowest at 5.6 mgO(2) g(-1) DW h(-1). Despite the low amplitude of seasonal variation in this south tropical area, the three species showed seasonal variation in the primary production: GPP was positively correlated with photosynthetic active radiation for U. foliosa and E. densa, and there was a negative correlation for C. furcata. For U. foliosa, GPP was positively correlated with temperature and dissolved inorganic carbon and the GPP of C. jurcata was positively correlated with dissolved inorganic carbon.

Ueber das Wachsthum von Metzgeria furcata und Aneura

Von K. Goebel

Breeding populations of seabirds in California, 1989-1991. Volume I - population estimates

In 1989-1991, the U.S. Fish and Wildlife Service surveyed breeding populations of seabirds on the entire California coast. This study was sponsored by the Minerals Management Service in relation to outer continental shelf oil and gas leasing. At 483 nesting sites (excluding terns and skimmers in southern California), we estimated 643,307 breeding birds of 21 seabird species including: 410 Fork-tailed Storm-petrel (Oceanodroma furcata); 12,551 Leach's Storm-petrel (O. leucorhoa); 7,209 Ashy Storm-petrel (O. homochroa); 274 Black Storm-petrel (O. melania); 11,916 Brown Pelican (Pelecanus occidentalis); 10,037 Double-crested Cormorant (Phalacrocorax auritus); 83,394 Brandt's Cormorant (P. penicillatus); 14,345 Pelagic Cormorant (P. pelagicus); 888 Black Oystercatcher (Haemotopus bachmani); 4,764 California Gull (Larus californicus); 61,760 Western Gull (L. occidentalis); 2,838 Caspian Tern (Sterna caspia) (excluding southern California); 3,550 Forster's Tern (S. forsteri) (excluding southern California); 272 Least Tern (S. albifrons) (excluding southern California); 351,336 Common Murre (Uria aalge); 15,470 Pigeon Guillemot (Cepphus columba); 1,821 Marbled Murrelet (Brachyramphus marmoratus); 1,760 Xantus' Murrelet (Endomychura hypoleuca); 56,562 Cassin's Auklet (Ptychoramphus aleuticus); 1,769 Rhinoceros Auklet (Cerorhinca monocerata); and 276 Tufted Puffin (Fratercula cirrhata). The inland, historical or hybrid breeding status of American White Pelican (P. erythrorynchus), American Oystercatcher (H. palliatus), Heermann's Gull (L. heermanni), Ring-billed Gull (L. delawarensis), Glaucous-winged Gull (L. glaucescens) and Black Tern (Chlidonias niger) are discussed. Estimates for Gull-billed Tern (S. nilotica), Royal Tern (S. maxima), Elegant Tern (S. elegans) and Black Skimmer (Rhynchops niger) will be included in the final draft of this report. Overall numbers were slightly lower than reported in 1975-1980 surveys (summarized in Sowls et al. 1980. Catalog of California seabird colonies. U.S. Dept. Int., Fish Wildl. Serv., Biol. Serv. Prog., FWS/OBS 37/80). Recent declines were found or suspected for Fork-tailed Storm-petrel, Leach's Storm-petrel, White Pelican, Black Tern, Caspian Tern, Least Tern, Common Murre and Marbled Murrelet. Recent increases were found or suspected for Brown Pelican, Double-crested cormorant, California Gull, Western Gull, Forster's Tern and Rhinoceros Auklet. Similar numbers were found for other species or trends could not be determined without additional surveys, studies and/or more in-depth comparisons with previous surveys. The status of terns and skimmers in southern California has not yet been finalized.

中国轮藻新植物和新记录

本文报道了轮藻科1新种、2新变种及4个中国新记录:不等丽藻 Nitella inaequabilis sP.nov,分叉丽藻齿网变种N.furcata var.dentireticulata var.nov.;普生轮藻等苞变种 Chara vulgaris var.aequibracteata var.nov.;裂顶丽藻 Nitella partita Nordst.;过渡丽藻N.transilis T.F.Allen;布洛丽藻 N.blowiana J.Grove;娇小丽藻 N.pulchella T.

Ecophysiological characteristics of four intertidal marine macroalgae during emersion along Shantou coast of China, with a special reference to the relationship of photosynthesis and CO2

Intertidal marine macroalgae experience periodical exposures during low tide due to their zonational distribution. The duration of such emersion leads to different exposures of the plants to light and aerial CO2, which then affect the physiology of them to different extents. The ecophysiological responses to light and CO2 were investigated during emersion in two red algae Gloiopeltis furcata and Gigartina intermedia, and two brown algae Petalonia fascia and Sargassum hemiphyllum, growing along the Shantou coast of China. The light-saturated net photosynthesis in G. furcata and P. fascia showed an increase followed by slightly desiccation, whereas that in G. intermedia and S. hemiphyllum exhibited a continuous decrease with water loss. In addition, the upper-zonated G. furcata and P. fascia, exhibited higher photosynthetic tolerance to desiccation and required higher light level to saturate their photosynthesis than the lower-zonated G. intemedia and S. hemiphyllum. Desiccation had less effect on dark respiration in these four algae compared with photosynthesis. The light-saturated net photosynthesis increased with increased CO2 concentrations, being saturated at CO2 concentrations higher than the present atmospheric level in G. furcata, G. intermedia and S. hemiphyllum during emersion. It was evident that the relative enhancement of photosynthesis by elevated CO, in those three algae increased, though the absolute values of photosynthetic enhancement owing to CO2 increase were reduced when the desiccation statuses became more severe. However, in the case of desiccated P. fascia (water loss being greater than 20 %), light saturated net photosynthesis was saturated with current ambient atmospheric CO2 level. It is proposed that increasing atmospheric CO2 will enhance the daily photosynthetic production in intertidal macroalgae by varied extents that were related to the species and zonation.

Estudi taxonòmic i ecofisiològic dels membres de la família faucheaceae (Rhodymeniales, Rhodophyta) de la Península Ibèrica i de les Illes Balears

L'objectiu d'aquesta tesi doctoral ha estat estudiar a nivell taxonòmic i ecofisiològic les espècies de la família Faucheaceae (Rhodymeniales, Rhodophyta) presents a la península Ibèrica i a les illes Balears: Fauchea repens (C. Agardh) Montagne i Bory in Montagne, Gloiocladia furcata (C. Agardh) J. Agardh i Gloiocladia microspora (Bornet ex Rodríguez y Femenías) Sánchez i Rodríguez-Prieto, comb. nov. L'estudi taxonòmic es va realitzar a partir de mostres recol·lectades al llarg de la costa de la península Ibérica i de les illes Balears, amb la revisió dels exemplars conservats en els herbaris de diferents universitats espanyoles i dels exemplars tipus localitzats a l'herbari de C. Agardh i al de J.J. Rodríguez y Femenías. Així doncs, amb aquest treball es van completar les dades ja existents quant a la morfologia, l'estructura vegetativa i els tetrasporòfits de les tres espècies i es van aportar dades inèdites pel què fa a les estructures reproductores masculines de Fauchea repens, les estructures reproductores femenines i estadis de postfertilització de F. repens, G. furcata i G. microspora comb. nov. Paral·lelament es va detallar la corologia, l'hàbitat i la fenologia de les tres espècies. De l'estudi de F. repens i G. furcata, espècies tipus dels seus gèneres i, en el cas de la primera també de la família, es proposa una correcció de la descripció original de la família Faucheaceae I.M. Strachan, G.W. Saunders & Kraft, així com una nova combinació Gloiocladia microspora comb. nov. per l'espècie anomenada fins aquest estudi Fauchea microspora Bornet ex Rodríguez y Femenías. S'aporta també una iconografia original de dibuixos i fotografies, i una clau de determinació dels tàxons estudiats. Quant a l'estudi ecofisiològic, es van realitzar una sèrie d'experiments de laboratori per tal de determinar els requeriments de llum, temperatura i fotoperíode per el creixement i supervivència de les tres espècies. Així doncs, es van aportar dades inèdites pel què fa al cultiu in vitro de Fauchea repens, Gloiocladia furcata i Gloiocladia microspora comb. nov., que ens van permetre explicar la distribució batimètrica i geogràfica de les tres espècies.

Reproductive Consequences of Nest Site Use in Fork-tailed Storm-Petrels in the Aleutian Islands, Alaska: Potential Lasting Effects of an Introduced Predator

We examined the reproductive consequences of differential nest site use in Fork-tailed Storm-Petrels (Oceanodroma furcata) in the Aleutian Islands, Alaska, where birds on islands where foxes were introduced nest in rocky substrate rather than in typical soil habitat. We investigated how physical and microclimatic nest site characteristics influenced storm-petrel breeding success 20 years after fox removal. We then examined whether those nest site characteristics that affected success were related to the amount of rock that composed the nest. In both years of our study, nest temperature had the strongest influence on chick survival and overall reproductive success, appearing in all the top models and alone explaining 14–35% of the variation in chick survival. The relationship between reproductive success and nest temperature was positive in both years, with higher survival in warmer nests. In turn, the best predictor of nest temperature was the amount of rock that composed the site. Rockier nests had colder average temperatures, which were driven by lower daily minimum temperatures, compared to nests with more soil. Thus, the rockiness of the nest site appeared to affect chick survival and overall reproductive success through its influence on nest temperature. This study suggests that the use of rocky nest sites, presumed to be a result of historic predation from introduced foxes, could decrease breeding success in this recovering population, and thus be a long-lasting effect of introduced predators.

A novel method for the transport and analysis of genetic material from polyps and zooxanthellae of scleractinian corals

We have developed a new simple method for transport, storage, and analysis of genetic material from the corals Agaricia agaricites, Dendrogyra cylindrica, Eusmilia ancora, Meandrina meandrites, Montastrea annularis, Porites astreoides, Porites furcata, Porites porites, and Siderastrea siderea at room temperature. All species yielded sufficient DNA from a single FTA(R) card (19 mug-43 ng) for subsequent PCR amplification of both coral and zooxanthellar DNA. The D1 and D2 variable region of the large Subunit rRNA gene (LSUrDNA) was amplified from the DNA of P. furcata and S. siderea by PCR. Electrophoresis yielded two major DNA bands: an 800-base pair (bp) DNA, which represented the coral ribosomal RNA (rRNA) gene, and a 600-bp DNA, which represented the zooxanthellar srRNA gene. Extraction of DNA from the bands yielded between 290 mug total DNA (S. siderea coral DNA) and 9 mug total DNA (P. furcata zooxanthellar DNA). The ability to transport and store genetic material from scleractinian corals without resort to laboratory facilities in the field allows for the molecular Study of a far wider range and variety of coral sites than have been studied to date. (C) 2003 Elsevier Science B.V. All rights reserved.

Territorial behaviour and dominance hierarchy of Anthracothorax nigricollis Vieillot 1817 (Aves: Trochilidae) on food resources

Dominant species are those which delimit and defend territories from other individuals of the same or different species. Subordinate species are those which, furtive and sneakily, use sources of nectar from other individuals. This study aimed to describe the aggressive interactions between species of hummingbirds, define which species are dominant and which are subordinate, investigate if the sharing of resources occurs during the visits, and compare the behaviour of the dominant species in different strata (tree, arbustive and herbaceous). The species observed interacting with Anthracothorax nigricollis Vieillot 1817 were Phaethornis pretrei Lesson and Delattrer 1839, Thalurania furcata Gmelin 1788, and Polytmus guainumbi Pallas 1764. Nine behavioural acts grouped into four categories were identified and described. The dominant species is A. nigricollis (with 0.9 of the attacks), followed by T. furcata (with 0.07) and P. pretrei (with 0.03). The resource sharing was seen only in the shrub layer, in C. surinamensis, in which there was intraspecific and interspecific sharing. A. nigricollis showed higher interspecific toleration, T. furcata (0.27) and P. pretrei (0.55) than intraspecific A. nigricollis (0.18). The frequency of occurrence of behaviours expressed by A. nigricollis in the three vegetation strata differed significantly.

Variações morfológicas em Edessa rufomarginata e revalidação de E. albomarginata e E. marginalis (Heteroptera, Pentatomidae, Edessinae)

ABSTRACT: Based on the types of Edessa rufomarginata (De Geer, 1773) and its synonyms, on morphology of the paramere and coloration, seven patterns are described for E. rufomarginata. Pentatoma furcata Palisot de Beauvois, 1805, Cimex cruentus Fabricius, 1775, Aceratodes flavovirens Stål, 1855 and A. flavomarginatus Stål, 1855 are mantained as junior synonyms of E. rufomarginata. A. albomarginatus Stål, 1855 and A. marginalis Dallas, 1951 are removed from the synonymy of E. rufomarginata and are reinstated in Edessa. Aceratodes discolor Dallas, 1951 is removed from the synonymy of E. rufomarginata and is considered junior synonym of Edessa abdominalis Erichson, 1848.

Distribuição de macrófitas aquáticas em relação a um gradiente ambiental na bacia do Rio Itanhaém, SP

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Testes oftálmicos, biometria ocular e cálculo do poder dióptrico da lente em corujas

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Redefining the taxonomic status of Laurencia dendroidea (Ceramiales, Rhodophyta) from Brazil and the Canary Islands

Morphological and molecular studies have been performed on Laurencia dendroidea derived from Brazil and the Canary Islands. This species possesses all of the characters that are typical of the genus Laurencia, including the production of the first pericentral cell underneath the basal cell of the trichoblast; the production of tetrasporangia from particular pericentral cells without the formation of additional fertile pericentral cells; spermatangial branches that are produced from one of two laterals on the suprabasal cell of the trichoblasts; and a procarp-bearing segment that possesses five pericentral cells. The phylogenetic position of L. dendroidea was inferred by analysing the chloroplast-encoded rbcL gene sequences of 51 taxa. Phylogenetic analyses revealed that the taxa previously identified and cited in Brazil as Laurencia filiformis, L. majuscula and L. obtusa and in the Canary Islands as L. majuscula all represent the same taxonomic entity and examination of type material allowed us to identify this entity as L. dendroidea, whose type locality is in Brazil. Laurencia obtusa from the Northern Atlantic is confirmed to represent a distinct species, which displays high genetic divergence with respect to western and eastern Atlantic samples. The phylogenetic analyses also supported the nomenclatural transfer of Chondrophycus furcatus (Cordeiro-Marino & M. T. Fujii) M. T. Fujii & Senties to Palisada furcata (Cordeiro-Marino & M. T. Fujii) Cassano & M. T. Fujii comb. nov.