Mitochondrial genome acquisition restores respiratory function and tumorigenic potential of cancer cells without mitochondrial DNA

We report that tumor cells devoid of their mitochondrial genome (mtDNA) show delayed tumor growth and that tumor formation is associated with acquisition of mtDNA from host cells. This leads to partial recovery of mitochondrial function in cells derived from primary tumors grown from cells without mtDNA and a shorter lag in tumor growth. Cell lines from circulating tumor cells showed further recovery of mitochondrial respiration and an intermediate lag to tumor growth, while cells from lung metastases exhibited full restoration of respiratory function and no lag in tumor growth. Stepwise assembly of mitochondrial respiratory supercomplexes was correlated with acquisition of respiratory function. Our findings indicate horizontal transfer of mtDNA from host cells in the tumor microenvironment to tumor cells with compromised respiratory function to re-establish respiration and tumor-initiating efficacy. These results suggest a novel pathophysiological process for overcoming mtDNA damage and support the notion of high plasticity of malignant cells.

A novel phantom and method for comprehensive 3-dimensional measurement and correction of geometric distortion in magnetic resonance imaging

A phantom that can be used for mapping geometric distortion in magnetic resonance imaging (MRI) is described. This phantom provides an array of densely distributed control points in three-dimensional (3D) space. These points form the basis of a comprehensive measurement method to correct for geometric distortion in MR images arising principally from gradient field non-linearity and magnet field inhomogeneity. The phantom was designed based on the concept that a point in space can be defined using three orthogonal planes. This novel design approach allows for as many control points as desired. Employing this novel design, a highly accurate method has been developed that enables the positions of the control points to be measured to sub-voxel accuracy. The phantom described in this paper was constructed to fit into a body coil of a MRI scanner, (external dimensions of the phantom were: 310 mm x 310 mm x 310 mm), and it contained 10,830 control points. With this phantom, the mean errors in the measured coordinates of the control points were on the order of 0.1 mm or less, which were less than one tenth of the voxel's dimensions of the phantom image. The calculated three-dimensional distortion map, i.e., the differences between the image positions and true positions of the control points, can then be used to compensate for geometric distortion for a full image restoration. It is anticipated that this novel method will have an impact on the applicability of MRI in both clinical and research settings. especially in areas where geometric accuracy is highly required, such as in MR neuro-imaging. (C) 2004 Elsevier Inc. All rights reserved.

Geometric distortion in clinical MRI systems - Part II: correction using a 3D phantom

In this paper, we present the correction of the geometric distortion measured in the clinical magnetic resonance imaging (MRI) systems reported in the preceding paper (Part 1) using a 3D method based on the phantom-mapped geometric distortion data. This method allows the correction to be made on phantom images acquired without or with the vendor correction applied. With the vendor's 2D correction applied, the method corrects for both the residual geometric distortion still present in the plane in which the correction method was applied (the axial plane) and the uncorrected geometric distortion along the axis non-nal to the plane. The evaluation of the effectiveness of the correction using this new method was carried out through analyzing the residual geometric distortion in the corrected phantom images. The results show that the new method can restore the distorted images in 3D nearly to perfection. For all the MRI systems investigated, the mean absolute deviations in the positions of the control points (along x-, y- and z-axes) measured on the corrected phantom images were all less than 0.2 mm. The maximum absolute deviations were all below similar to0.8 mm. As expected, the correction of the phantom images acquired with the vendor's correction applied in the axial plane performed equally well. Both the geometric distortion still present in the axial plane after applying the vendor's correction and the uncorrected distortion along the z-axis have all been restored. (C) 2004 Elsevier Inc. All rights reserved.

Valuation of Ecosystem Services for Environmental Decision Making in South Florida

The Greater Everglades system imparts vital ecosystem services (ES) to South Florida residents including high quality drinking water supplies and a habitat for threatened and endangered species. As a result of the altered Everglades system and regional dynamics, restoration may either improve the provision of these services or impose a tradeoff between enhanced environmental goods and services and competing societal demands. The current study aims at understanding public preferences for restoration and generating willingness to pay (WTP) values for restored ES through the implementation of a discrete choice experiment. A previous study (Milon et al., 1999) generated WTP values amongst Floridians of up to $3.42 -$4.07 billion for full restoration over a 10-year period. We have collected data from 2,905 respondents taken from two samples who participated in an online survey designed to elicit the WTP values for selected ecological and social attributes included in the earlier study (Milon et al. 1999). We estimate that the Florida general public is willing to pay up to $854.1- $954.1 million over 10 years to avoid restrictions on their water usage and up to $90.8- $183.7 million over 10 years to restore the hydrological flow within the Water Conservation Area.

La liquidación de las instituciones corporativas coloniales en tiempos de la independencia : la Reducción de San Ignacio de los Tobas, Jujuy

Este trabajo estudia el impacto de las nuevas concepciones económicas sobre las estructuras agrarias corporativas introducidas en el Río de la Plata desde la revolución. El caso a analizar es la Reducción indígena de San Ignacio de los Tobas en la frontera oriental con el Chaco, que experimentó un proceso de liquidación de tierras entre 1816 y 1821. En ese proceso se enfrentaron dos discursos en torno al destino de este tipo de instituciones corporativas: uno que proponía su desmantelamiento como entidades jurídicas y la venta de sus tierras a particulares, el otro que sostenía el restablecimiento de su pleno funcionamiento, aunque dentro de un marco republicano que terminó horadando la legitimidad de origen de la Reducción indígena.

La liquidación de las instituciones corporativas coloniales en tiempos de la independencia : la Reducción de San Ignacio de los Tobas, Jujuy

Este trabajo estudia el impacto de las nuevas concepciones económicas sobre las estructuras agrarias corporativas introducidas en el Río de la Plata desde la revolución. El caso a analizar es la Reducción indígena de San Ignacio de los Tobas en la frontera oriental con el Chaco, que experimentó un proceso de liquidación de tierras entre 1816 y 1821. En ese proceso se enfrentaron dos discursos en torno al destino de este tipo de instituciones corporativas: uno que proponía su desmantelamiento como entidades jurídicas y la venta de sus tierras a particulares, el otro que sostenía el restablecimiento de su pleno funcionamiento, aunque dentro de un marco republicano que terminó horadando la legitimidad de origen de la Reducción indígena.

La liquidación de las instituciones corporativas coloniales en tiempos de la independencia : la Reducción de San Ignacio de los Tobas, Jujuy

Este trabajo estudia el impacto de las nuevas concepciones económicas sobre las estructuras agrarias corporativas introducidas en el Río de la Plata desde la revolución. El caso a analizar es la Reducción indígena de San Ignacio de los Tobas en la frontera oriental con el Chaco, que experimentó un proceso de liquidación de tierras entre 1816 y 1821. En ese proceso se enfrentaron dos discursos en torno al destino de este tipo de instituciones corporativas: uno que proponía su desmantelamiento como entidades jurídicas y la venta de sus tierras a particulares, el otro que sostenía el restablecimiento de su pleno funcionamiento, aunque dentro de un marco republicano que terminó horadando la legitimidad de origen de la Reducción indígena.

A full and circumstantial account of the memorable battle of Waterloo, the second restoration of Louis XVIII, and the deportation of Napoleon Buonaparte to the island of St. Helena, and every recent particular relative to his conduct and mode of life in his exile, together with an interesting account of the affairs of France, and biographical sketches of the most distinguished Waterloo heroes /

Includes index.

Regularized iterative and non-iterative procedures for object restoration from experimental data

A regularized algorithm for the recovery of band-limited signals from noisy data is described. The regularization is characterized by a single parameter. Iterative and non-iterative implementations of the algorithm are shown to have useful properties, the former offering the advantage of flexibility and the latter a potential for rapid data processing. Comparative results, using experimental data obtained in laser anemometry studies with a photon correlator, are presented both with and without regularization. © 1983 Taylor & Francis Ltd.

Regularized iterative and noniterative procedures for object restoration in the presence of noise :an error analysis

An analysis is carried out, using the prolate spheroidal wave functions, of certain regularized iterative and noniterative methods previously proposed for the achievement of object restoration (or, equivalently, spectral extrapolation) from noisy image data. The ill-posedness inherent in the problem is treated by means of a regularization parameter, and the analysis shows explicitly how the deleterious effects of the noise are then contained. The error in the object estimate is also assessed, and it is shown that the optimal choice for the regularization parameter depends on the signal-to-noise ratio. Numerical examples are used to demonstrate the performance of both unregularized and regularized procedures and also to show how, in the unregularized case, artefacts can be generated from pure noise. Finally, the relative error in the estimate is calculated as a function of the degree of superresolution demanded for reconstruction problems characterized by low space–bandwidth products.

On the problems of object restoration and image extrapolation in optics

In this paper we consider the problems of object restoration and image extrapolation, according to the regularization theory of improperly posed problems. In order to take into account the stochastic nature of the noise and to introduce the main concepts of information theory, great attention is devoted to the probabilistic methods of regularization. The kind of the restored continuity is investigated in detail; in particular we prove that, while the image extrapolation presents a Hölder type stability, the object restoration has only a logarithmic continuity. © 1979 American Institute of Physics.

Object Restoration Beyond the Rayleigh Limit in the Presence of Noise

info:eu-repo/semantics/published

Resolution beyond the diffraction limit for regularized object restoration

We propose a new formulation of Miller's regularization theory, which is particularly suitable for object restoration problems. By means of simple geometrical arguments, we obtain upper and lower bounds for the errors on regularized solutions. This leads to distinguish between ' Holder continuity ' which is quite good for practical computations and ` logarithmic continuity ' which is very poor. However, in the latter case, one can reconstruct local weighted averages of the solution. This procedure allows for precise valuations of the resolution attainable in a given problem. Numerical computations, made for object restoration beyond the diffraction limit in Fourier optics, show that, when logarithmic continuity holds, the resolution is practically independent of the data noise level. © 1980 Taylor & Francis Group, LLC.

Cellular immune responses in human immunodeficiency virus (HIV)-1-infected children: Is immune restoration by highly active anti-retroviral therapy comparable to non-progression?

The objective of this study was to investigate whether the restored immune functions of vertically human immunodeficiency virus (HIV)-infected children who were severely immunodeficient before the initiation of highly active anti-retroviral therapy (HAART) are comparable to those of untreated slow progressors. We therefore assessed T cell proliferation and cytokine [interferon (IFN)-γ, interleukin (IL)-5 and IL-13] secretions after mitogen, recall antigens and HIV-1-specific stimulation in 12 untreated slow progressors, 16 untreated progressors and 18 treated patients. Treated children were profoundly immunodeficient before the initiation of HAART and had long-lasting suppression of viral replication on treatment. We demonstrated that slow progressors are characterized not only by the preservation of HIV-1-specific lymphoproliferative responses but also by the fact that these responses are clearly T helper type 1 (Th1)-polarized. Children on HAART had proliferative responses to HIV-1 p24 antigen, purified protein derivative (PPD) and tetanus antigen similar to slow progressors and higher than those of progressors. However, in contrast to slow progressors, most treated children exhibited a release of Th2 cytokines accompanying the IFN-γ secretion in response to the HIV-1 p24 antigen. Moreover, despite higher proliferative responses to phytohaemagglutinin (PHA) than the two groups of untreated children, treated children had lower levels of IFN-γ secretion in response to PHA than slow progressors. These data show that in severely immunodeficient vertically HIV-infected children, a long-lasting HAART allows recovering lymphoproliferative responses similar to untreated slow progressors. However, alterations in IFN-γ secretion in response to the mitogen PHA persisted, and their cytokine release after HIV-specific stimulation was biased towards a Th2 response. © 2011 The Authors. Clinical and Experimental Immunology © 2011 British Society for Immunology.

Restoration of optical objects using regularization

Using the regularization theory for improperly posed problems, we discuss object restoration beyond the diffraction limit in the presence of noise. Only the case of one-dimensional coherent objects is considered. We focus attention n the estimation of the error on the restored objects, and we show that, in most realistic cases, it is at best proportional to an inverse power of