Age and growth of the estuarine dolphin (Sotalia guianensis) (Cetacea, Delphinidae) on the Paran�� Coast, southern Brazil

Teeth of 71 estuarine dolphins (Sotalia guianensis) incidentally caught on the coast of Paran�� State, southern Brazil, were used to estimate age. The oldest male and female dolphins were 29 and 30 years, respectively. The mean distance from the neonatal line to the end of the first growth layer group (GLG) was 622.4 ��19.1 ��m (n=48). One or two accessory layers were observed between the neonatal line and the end of the first GLG. One of the accessory layers, which was not always present, was located at a mean of 248.9 ��32.6 ��m (n=25) from the neonatal line, and its interpretation remains uncertain.The other layer, located at a mean of 419.6 ��44.6 ��m (n=54) from the neonatal line, was always present and was first observed between 6.7 and 10.3 months of age. This accessory layer could be a record of weaning in this dolphin. Although no differences in age estimates were observed between teeth sectioned in the anterior-posterior and buccal-lingual planes, we recommend sectioning the teeth in the buccal-lingual plane in order to obtain on-center sections more easily. We also recommend not using teeth from the most anterior part of the mandibles for age estimation. The number of GLGs counted in those teeth was 50% less than the number of GLGs counted in the teeth from the median part of the mandible of the same animal. Although no significant difference (P>0.05) was found between the total lengths of adult male and female estuarine dolphins, we observed that males exhibited a second growth spurt around five years of age. This growth spurt would require that separate growth curves be calculated for the sexes. The asymptotic length (TL���), k, and t0 obtained by the von Bertalanffy growth model were 177.3 cm, 0.66, and ���1.23, respectively, for females and 159.6 cm, 2.02, and ���0.38, respectively, for males up to five years, and 186.4 cm, 0.53 and ���1.40, respectively, for males older than five years. The total weight (TW)/total length (TL) equations obtained for male and female estuarine dolphins were TW = 3.156 �� 10���6 �� TL 3.2836 (r=0.96), and TW = 8.974 �� 10���5 �� TL 2.6182 (r=0.95), respectively.

Efficiency of wear and decalcification technique for estimating the age of estuarine dolphin Sotalia guianensis

Most techniques used for estimating the age of Sotalia guianensis (van B,n,den, 1864) (Cetacea; Delphinidae) are very expensive, and require sophisticated equipment for preparing histological sections of teeth. The objective of this study was to test a more affordable and much simpler method, involving of the manual wear of teeth followed by decalcification and observation under a stereomicroscope. This technique has been employed successfully with larger species of Odontoceti. Twenty-six specimens were selected, and one tooth of each specimen was worn and demineralized for growth layers reading. Growth layers were evidenced in all specimens; however, in 4 of the 26 teeth, not all the layers could be clearly observed. In these teeth, there was a significant decrease of growth layer group thickness, thus hindering the layers count. The juxtaposition of layers hindered the reading of larger numbers of layers by the wear and decalcification technique. Analysis of more than 17 layers in a single tooth proved inconclusive. The method applied here proved to be efficient in estimating the age of Sotalia guianensis individuals younger than 18 years. This method could simplify the study of the age structure of the overall population, and allows the use of the more expensive methodologies to be confined to more specific studies of older specimens. It also enables the classification of the calf, young and adult classes, which is important for general population studies.

A GIS analysis of coastal development and trends in bottlenose dolphin strandings in Charleston, SC: implications for coastal marine spatial planning

Bottlenose dolphins (Tursiops truncatus) inhabit estuarine waters near Charleston, South Carolina (SC) feeding, nursing and socializing. While in these waters, dolphins are exposed to multiple direct and indirect threats such as anthropogenic impacts (egs. harassment with boat traffic and entanglements in fishing gear) and environmental degradation. Bottlenose dolphins are protected under the Marine Mammal Protection Act of 1972. Over the years, the percentage of strandings in the estuaries has increased in South Carolina and, specifically, recent stranding data shows an increase in strandings occurring in Charleston, SC near areas of residential development. During the same timeframe, Charleston experienced a shift in human population towards the coastline. These two trends, rise in estuarine dolphin strandings and shift in human population, have raised questions on whether the increase in strandings is a result of more detectable strandings being reported, or a true increase in stranding events. Using GIS, the trends in strandings were compared to residential growth, boat permits, fishing permits, and dock permits in Charleston County from 1994-2009. A simple linear regression analysis was performed to determine if there were any significant relationships between strandings, boat permits, commercial fishing permits, and crabpot permits. The results of this analysis show the stranding trend moves toward Charleston Harbor and adjacent rivers over time which suggests the increase in strandings is related to the strandings becoming more detectable. The statistical analysis shows that the factors that cause human interaction strandings such as boats, commercial fishing, and crabpot line entanglements are not significantly related to strandings further supporting the hypothesis that the increase in strandings are due to increased observations on the water as human coastal population increases and are not a natural phenomenon. This study has local and potentially regional marine spatial planning implications to protect coastal natural resources, such as the bottlenose dolphin, while balancing coastal development.

Avalia����o da exposi����o de botos-cinza (Sotalia guianensis Van Ben��d��n, 1864) aos compostos org��nicos de estanho atrav��s das concentra����es hep��ticas de estanho total na costa sudeste e sul do Brasil

Durante as ��ltimas d��cadas, observou-se um aumento da preocupa����o em rela����o aos ecossistemas marinhos devido �� grande entrada de poluentes, resultando em efeitos delet��rios em organismos aqu��ticos e seres humanos. Dentre as atividades humanas que podem introduzir compostos t��xicos persistentes e bioacumulativos (PBTs Persistent Bioaccumulative Toxicants) no ambiente marinho est�� o uso de tintas antiincrustrantes, aplicadas nos cascos de navios para evitar que algas, mexilh��es e outros organismos se fixem ��s embarca����es. N��o raramente, compostos organoest��nicos (OTs) como o Tributilestanho (TBT) ou o Trifenilestanho (TPT) constitu��am o princ��pio ativo de tal preparado. Devido �� alta toxicidade desses compostos, a IMO (Organiza����o Mar��tima Internacional) baniu totalmente o uso dos mesmos. Como os OTs s��o prontamente bioacumulados, elevadas concentra����es de estanho total (SnT) v��m sendo encontradas em cet��ceos (Mammalia, Cetacea). Os botos-cinza (Sotalia guianensis Van Beneden, 1864) ocupam elevados n��veis tr��ficos e bioacumulam os PBTs aos quais est��o expostos. Alguns autores relataram que o estanho hep��tico em cet��ceos se encontra predominantemente na forma org��nica, visto que, na forma inorg��nica tal metal �� pobremente absorvido pela mucosa gastrintestinal, de forma que as concentra����es hep��ticas de SnT refletem o input antr��pico de OTs. O presente estudo teve como principal objetivo, avaliar a exposi����o de botos-cinza aos OTs, atrav��s determina����o das concentra����es hep��ticas de estanho total (SnT = org��nico + inorg��nico), por Espectrometria de Absor����o At��mica com Atomiza����o em Forno de Grafite (GFAAS Graphite Furnace Atomic Absorption Spectrometry). Para tal, amostras de botos-cinza de diferentes ��reas do litoral brasileiro, compreendendo a Regi��o da Grande Vit��ria (GV), Ba��a de Guanabara (BG), Ba��a de Sepetiba (B.Sep), a Ba��a de Paranagu�� (PR) e a Ba��a da Babitonga (SC), foram analisadas, visando comparar ambientes distintamente contaminados com OTs. Sendo assim, as concentra����es hep��ticas de SnT (em ng/g, peso seco) de botos-cinza variaram de <312 (limite de detec����o) a 8.250, para a GV (n=22); de <312 a 14.100, para B.Sep (n = 38); <312 a 5.147, para PR (n= 22), bem como de 626 a 24.780 (ng/g, peso seco) para os botos de SC (n=10). As maiores concentra����es foram verificadas nos botos da BG (n=11), variando de 1.265 a 24.882 (ng/g, peso seco). As concentra����es encontradas na Ba��a de Guanabara (BG) est��o entre as mais elevadas detectadas em cet��ceos.

Etnobiologia do boto cinza (Sotalia guianensis, van B��n��den, 1864) por comunidades pesqueiras do Rio Grande do Norte, Basil

Ethnobiology studies Local Ecological Knowledge (LEK) as well as the use and management of natural resources by local communities in order to understand how the environment is perceived, known and classified by human groups. In fishing communities, LEK adds empirical information about the biology of aquatic species and complements scientific findings, especially when it is difficult to obtain factual information during studies on cetaceans, whose behavior is essentially underwater. Cetaceans (whales and dolphins) are constantly threatened by human activities, especially by accidental capture of small coastal species, as in the case of the estuarine dolphin (Sotalia guianensis), object of this study. Ethnobiological researches in fishing communities are of great importance and can clarify aspects of the biology and conservation of this species. Although extensively studied throughout its distribution range, there are still gaps in the knowledge about S. guianensis. Therefore, fishers local ecological knowledge becomes an additional tool to get and confirm information about S. guianensis. This study evaluated the LEK of artisanal fishers who are daily exposed to local population of S. guianensis, through the use of semistructured interviews (N=116). The interviewed fishers were asked about the biology and popular classification of S. guianensis and about possible interactions between this dolphin and them. The studied communities were located in Tibau do Sul (n=39), Pipa (n=36) and Ba��a Formosa (n=41), all on the south coast of Rio Grande do Norte, Brazil. The study was based on the assumption that differences in fishers LEK among those communities would be caused by both the variety of fishing environments (lagoon/estuary and ocean) and the intensity of tourism activities. Fishers knowledge is expressive and differed among the studied communities depending on the topic investigated. Fishers correctly reported the habitat, distribution, seasonality and behavioral particularities of S. guianensis. Tourism mainly affected the naming of the species. The study results also suggest that marine fishers have greater knowledge about the species than the estuarine/lagoon ones. Local populations accumulate empirical knowledge according to their environment. Hence, it is important to take into account both empirical knowledge and popular participation in management systems, in order to maintain information sharing among communities

Ecologia alimentar do boto cinza, sotalia guianensis (van ban��d��n, 1864), no litoral do rio grande do norte (rn)

This study investigated factors that influence the group size during the behavior foraging of estuarine dolphin Sotalia guianensis in the coast south of the state Rio Grande do Norte (RN), it also characterized the diet of estuarine dolphins and elaborated an otoliths catalog for aid in the identification of the preys found in the stomach contents. In relation to the group size during behaviour foraging, inside of the Curral Bay, larger frequency of solitary hunt was observed. Factors as tide variation, group composition and seasons didn't present correlation with the number of animals observed during a feeding episode. Capture success for participant was shown significantly larger when the animals hunted alone, what possibly explains the largest frequency of solitary hunt inside of the Curral Bay. About diet, were identified 18 bone fishes species, with predominance of species the families Haemulidae and Sciaenidae and five cephalopods species, including two new species in the diet of Sotalia guianensis in Brazil. Our results indicated that estuarine dolphin in the coast oriental from Rio Grande do Norte (RN) feeds predominantly of fish that form shoal, of habitat estuarino and producing of sounds. The analysis of the images, otoliths of 43 species of coastal fish of the coast of the state of Rio Grande do Norte (RN), aided by the offered descriptions, demonstrated to be an effective methodology for the knowledge of those structures, as well as it represented a form of reducing the subjectivity in the identification of the bone fishes found in stomach content

Varia����o di��ria e anual de freq����ncia e atividade do Boto Cinza Sotalia guianensis em Pipa, Nordeste do Brasil

The aim of this study was to investigate the daily and annual variation in frequency and in activity of an estuarine dolphin population, Sotalia guianensis, in Brazilian northeastern. Thus we estimated the daily variation and seasonality in frequency of adult and immature and in behavior of socialization, aerial activity, foraging of a boto equatorial population in the Curral inlet (6��13 00 S; 35��3 36 W). We detect some variation. Thus we associate the daily variation with daily period, tide fluctuation and annual season. And the seasonality variation we associate with the fotoper��odo and precipitation variation. From October 1999 to September 2003, through collect four times at month, we made instantaneous record at each five minute in six hours of survey. The surveys hours were ranged into light time of 24 hours, that is, between 06:00 and 17:00 o clock. These observations were developed simultaneous for two observers in a land-based survey at coastline cliff top of which observer have a fully visualization of whole inlet. We found that animals were more active and frequent during the morning. They were more active during low tide than high tide. However they did not show association with tide. We found yet that in a period from June to November (winter-spring) there was the highest socialization, aerial activity and foraging frequency. However adult and immature individual number did not have significance variation along the year. The larger concentration of this behaviors were not associate with photoperiod neither precipitation in the region. These results showed the existence of behavioral daily variation and seasonality in a species that live in equatorial region

Comportamento de mergulho do boto-cinza, Sotalia guianensis, na enseada do Curral, Praia de Pipa-RN, Brasil :poss��veis adapta����es card��acas ao mergulho

The marine tucuxi, Sotalia guianensis, is one of the smallest known cetaceans, has coastal habits, and occurs from Hondures to Santa Catarina, in southern Brazil. The objective of this dissertation was to describe diving behavior of the marine tucuxi in three age classes and to analyze the cardiac capacity to dive through the examination of hearts of stranded specimens. Observations were made from October 2004 to November 2005 from a vantage point, in Curral Bay at Pipa beach-RN. We used Ad Libitum sampling and All occurrences to record the behaviors. The diving was characterized by the total exposition of the tail fluke for a few seconds, in 90�� or 45�� angles. Were recorded 131 dives in three behavioral contexts: foraging, traveling and socialization. The difference between juveniles and adults in dive time and fluke out at 45�� or 90�� to search and/or capture prey is probably influenced by the strategy used and ability to capture the prey. The frequency of fluke out at 90�� for foraging in adults may be related to increased physiological efficiency of adults in comparison to juveniles. However, in the context of travel and socialization the dive time and fluke out were independent between the age classes. Dive in calves were frequent during socialization (play behavior) and traveling. This, associated with synchronic calve-adult diving suggests that a relationship of these behaviors and the acquisition of experience and foraging skills. As observed in other cetaceans, the heart (n=12) of the estuarine dolphin is broad and presents long ventricles which form a round apex. The right ventricle is long and narrow. The degree of dilatation of the aortic bulb may support the heart during diastole. The characteristic morphology of the heart and short dive duration < 2 min and depth ranged from 10m in the estuarine dolphin, can be likely at physiological adaptation for diving, typical de dolphins with coastal habits. The limitation of diving time in this specie may be influenced by anatomical and physiological restrictions

Sincronismo comportamental em Boto cinza(Sotalia guianensis)

Synchronism is the efetivation of the same behavior at the same time by two or more individuals. In cetology only from 2000 we verify an increasing of efforts for studies of synchronic behaviors. The synchronism in cetaceans was reported for a variety of contexts, including feeding, socializing, parental care, while resting, traveling and play, during afilliation, in sexual behaviour and possible while teaching. The synchronism in behavior is influenced by environmental factors and can be modified during stress situations such as the presence of boats. The aim of this study is to explore behavior synchronism presented by the estuarine dolphin (Sotalia guianensis) in Pipa, Rio Grande do Norte state, Northeastern Brazil. A total of 414 hours of observation during the year 2006, using scan sampling method, from a land based sighting was conducted. Analyses were conducted in four steps. Firstly, we determined the pattern of activity of the groups within the bay, testing for the influence of bay configuration before and after rains periods on the activity of animals. Analyses show that forage occurs more frequently after the rainy period, but there was no change in the number of individuals or spatial cohesions between them. Secondly, we analysed the influence of turism boat traffic on the behavior of animals. We verified no changes on the bahavior of animals during the presence of boats. Finally, we tested seven hypotheses regarding the ecological and social dynamics of synchronic behavior. Environmental aspects were considered the main influence for the synchronism, no influences of age, and more selective exihibition of synchronic breathing in feeding and social displays were observed during the study period

Timing of ontogenetic changes of two cranial regions in Sotalia guianensis (Delphinidae)

Despite the fact that heterochronic processes seem to be an important process determining morphological evolution of the delphinid skull, previous workers have not found allometric scaling as relevant factor in the differentiation within the genus Sotalia. Here we analyzed the skull ontogeny of the estuarine dolphin S. guianensis and investigate differential growth and shape changes of two cranial regions the neurocranium and the face in order to evaluate the relevance of cranial compartmentalization on the ontogeny of this structure. Our results show that, even though both cranial regions stop growing at adulthood, the face has higher initial growth rates than the neurocranium. The rate of shape changes is also different for both regions, with the face showing a initially higher, but rapidly decreasing rate of change, while the neurocranium shows a slow decreasing rate, leading to persistent and localized shape changes throughout adult life, a pattern that could be related to epigenetic regional factors. The pattern of ontogenetic shape change described here is similar to those described for other groups of Delphinidae and also match intra and interspecific variation found within the family, suggesting that mosaic heterochrony could be an important factor in the morphological evolution of this group. (C) 2012 Deutsche Gesellschaft fur Saugetierkunde. Published by Elsevier GmbH. All rights reserved.

Temporal and spatial aspects of bottlenose dolphin occurrence in coastal and estuarine waters near Charleston, South Carolina

The spatial and temporal occurrence of Atlantic bottlenose dolphins (Tursiops truncatus) in the coastal and estuarine waters near Charleston, SC were evaluated. Sighting and photographic data from photo-identification (ID), remote biopsy, capture-release and radio-tracking studies, conducted from 1994 through 2003, were analyzed in order to further delineate residence patterns of Charleston area bottlenose dolphins. Data from 250 photo-ID, 106 remote biopsy, 15 capture-release and 83 radio-tracking surveys were collected in the Stono River Estuary (n = 247), Charleston Harbor (n = 86), North Edisto River (n = 54), Intracoastal Waterway (n = 26) and the coastal waters north and south of Charleston Harbor (n = 41). Coverage for all survey types was spatially and temporally variable, and in the case of biopsy, capture-release and radio-tracking surveys, data analyzed in this report were collected incidental to other research. Eight-hundred and thirty-nine individuals were photographically identified during the study period. One-hundred and fifteen (13.7%) of the 839 photographically identified individuals were sighted between 11-40 times, evidence of consistent occurrence in the Charleston area (i.e., site fidelity). Adjusted sighting proportions (ASP), which reflect an individual���s sighting frequency in a subarea relative to other subareas after adjusting for survey effort, were analyzed in order to evaluate dolphin spatial occurrence. Forty-three percent (n = 139) of dolphins that qualified for ASP analyses exhibited a strong subarea affiliation while the remaining 57% (n = 187) showed no strong subarea preference. Group size data were derived from field estimates of 2,342 dolphin groups encountered in the five Charleston subareas. Group size appeared positively correlated with degree of ���openness��� of the body of water where dolphins were encountered; and for sightings along the coast, group size was larger during summer months. This study provides valuable information on the complex nature of bottlenose dolphin spatial and temporal occurrence near Charleston, SC. In addition, it helps us to better understand the stock structure of dolphins along the Atlantic seaboard.

Protocols for conducting dolphin capture-release health assessment studies

Marine mammals, such as dolphins, can serve as key indicator species in coastal areas by reflecting the effects of natural and anthropogenic stressors. As such they are often considered sentinels of environmental and ecosystem health (Bossart 2006; Wells et al. 2004; Fair and Becker 2000). The bottlenose dolphin is an apex predator and a key component of many estuarine environments in the southeastern United States (Woodward-Clyde Consultants 1994; SCDNR 2005). Health assessments of dolphins are especially critical in areas where populations are depleted, show signs of epidemic disease and/or high mortality and/or where habitat is being altered or impacted by human activities. Recent assessments of environmental conditions in the Indian River Lagoon, Florida (IRL) and the estuarine waters surrounding Charleston, South Carolina (CHS) highlight the need for studies of the health of local bottlenose dolphins. While the condition of southeastern estuaries was rated as fair in the National Coastal Condition Report (U.S. EPA 2001), it was noted that the IRL was characterized by poorer than expected benthic communities, significant sediment toxicity and increased nutrient concentrations. Similarly, portions of the CHS estuary have sediment concentrations of aliphatic aromatic hydrocarbons, select inorganic metals, and some persistent pesticides far in excess of reported bioeffect levels (Hyland et al. 1998). Long-term trends in water quality monitoring and recent scientific research suggest that waste load assimilation, non-point source runoff impacts, contaminated sediments, and toxic pollutants are key issues in the CHS estuary system. Several ���hot spots��� with high levels of heavy metals and organic compounds have been identified (Van Dolah et al. 2004). High concentrations of anthropogenic trace metals, polychlorinated biphenyls (PCB���s) and pesticides have been found in the sediments of Charleston Harbor, as well as the Ashley and Cooper Rivers (Long et al. 1998). Two superfund sites are located within the CHS estuary and the key contaminants of concern associated with these sites are: polycyclic aromatic hydrocarbons (PAH), lead, chromium, copper, arsenic, zinc and dioxin. Concerns related to the overall health of IRL dolphins and dermatologic disease observed in many dolphins in the area (Bossart et al. 2003) initiated an investigation of potential factors which may have impacted dolphin health. From May-August 2001, 35 bottlenose dolphins died in the IRL during an unusual mortality event (MMC 2003). Many of these dolphins were diagnosed with a variety of skin lesions including proliferative ulcerative dermatitis due to protozoa and fungi, dolphin pox and a vesicular dermatopathy of unknown etiology (Bossart et al. 2003). Multiple species from fish to dolphins in the IRL system have exhibited skin lesions of various known and unknown etiologies (Kane et al. 2000; Bossart et al. 2003; Reif et al. 2006). On-going photo-identification (photo-ID) studies have documented skin diseases in IRL dolphins (Mazzoil et al. 2005). In addition, up to 70% of green sea turtles in the IRL exhibit fibropapillomas, with the highest rates of occurrence being seen in turtles from the southern IRL (Hirama 2001).

EVIDENCE of A FAILED PREDATION ATTEMPT on A GUIANA DOLPHIN, Sotalia guianensis, BY A BULL SHARK, Carcharhinus leucas, IN BRAZILIAN WATERS.

Even though shark-cetacean interactions have been the subject of numerous studies worldwide, several ecological aspects such as competition, predation risk and co-evolution remain unclear. on February 16th, 2008, during a photo-identification survey to investigate population parameters of Guiana dolphins, Sotalia guianensis, in estuarine waters of Parana State (25[degree]S; 48[degree]W), Brazil, an adult dolphin was photographed without its dorsal fin. A detailed analysis of the healed area on the injured dolphin showed that the circular, crescent-shaped outlined wound was provoked by the bite of a bull shark, Carcharhinus leucas. Wound shape, prey-predator distributional patterns (sympatry) and feeding habits of the shark species here considered were indicative of the species' identity. The wound is likely to be the result of a failed predation attempt. Interactions between C. leucas and S. guianensis should be expected, since they are sympatric along almost all of their distribution range in the tropical and subtropical western South Atlantic. The presented observation adds S. guianensis to the list of cetacean species involved in interactions with large coastal predatory sharks.

Group size and composition of Guiana dolphins (Sotalia guianensis) (Van B��n��den, 1864) in the Paranagu�� Estuarine Complex, Brazil

Funda����o de Amparo �� Pesquisa do Estado de S��o Paulo (FAPESP)