Mutagenic activity removal of selected disperse dye by photoeletrocatalytic treatment

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Electrochemical method for quantitative determination of trace amounts of disperse dye in wastewater

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Evaluation of the interactions of DNA with the textile dyes Disperse Orange 1 and Disperse Red 1 and their electrolysis products using an electrochemical biosensor

A disposable pencil graphite electrode modified with dsDNA was used in combination with square wave voltammetry in order to evaluate the interaction of DNA with the textile dyes Disperse Orange 1 (DO1) and Disperse Red 1 (DR1), and with the products of their electrolysis. Significant changes in the characteristic oxidation peaks of the guanine and adenine moieties of immobilized dsDNA were observed after incubation of the modified electrode for 180 s in solutions of the dyes in their original forms. The same was observed using the electrolysis products obtained by oxidation and reduction conversions. The oxidation peak currents of the guanine and adenine moieties decreased when the concentrations of DO1 and DR1 were increased up to 5.0 × 10 -6 and 1.0 × 10-6 mol L-1, respectively; the signal decreases were more pronounced after interaction with the oxidized dyes, compared to the reduced compounds. The interactions between DNA and DO1, DR1, and the electrolyzed dyes were further investigated by UV-vis spectrophotometry in solution, and different effects such as hypochromism and hyperchromism were observed in the resulting DNA spectra. The investigated interactions showed clear evidence of changes in the DNA structure, and suggested a predominant intercalation mode leading to damage in the biomolecule. © 2013 Elsevier B.V.

Polymeric materials based on silk proteins

Silks are protein-based fibers made by arthropods for a variety of task-specific applications. In this article, we review the key features of silk proteins. This article initially focuses on the structure and function of silk proteins produced naturally by silkworms and spiders, followed by the biological and technical processing of silk proteins into a variety of morphologies (including capsules, fibers, films, foams, gels and spheres). Finally, we highlight the potential applications of silk-based materials. 

Degradação fotoeletroquímica de corantes dispersos em efluente têxtil utilizando fotoanodos de Ti/TiO2

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Determinação cromatográfica e degradação fotoeletrocatalítica do corante disperso Dispersol Black CVS

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Remoção de corantes utilizando tensoativos: extração por ponto de nuvem e floculação iônica

The textile sector is one of the main contributors to the generation of industrial wastewaters due to the use of large volumes of water, which has a high organic load content. In these, it is observed to the presence of dyes, surfactants, starch, alcohols, acetic acid and other constituents, from the various processing steps of the textiles. Hence, the treatment of textile wastewater becomes fundamental before releasing it into water bodies, where they can cause disastrous physical-chemical changes for the environment. Surfactants are substances widely used in separation processes and their use for treating textile wastewaters was evaluated in this research by applying the cloud point extraction and the ionic flocculation. In the cloud point extraction was used as surfactant nonylphenol with 9.5 ethoxylation degree to remove reactive dye. The process evaluation was performed in terms of temperature, surfactant and dye concentrations. The dye removal reached 91%. The ionic flocculation occurs due to the presence of calcium, which reacts with anionic surfactant to form insoluble surfactants capable of attracting the organic matter by adsorption. In this work the ionic flocculation using base soap was applied to the treatment of synthetic wastewater containing dyes belonging to three classes: direct, reactive, and disperse. It was evaluated by the influence of the following parameters: surfactant and electrolyte concentrations, stirring speed, equilibrium time, temperature, and pH. The flocculation of the surfactant was carried out in two ways: forming the floc in the effluent itself and forming the floc before mixing it to the effluent. Removal of reactive and direct dye, when the floc is formed into textile effluent was 97% and 87%, respectively. In the case where the floc is formed prior to adding it to the effluent, the removal to direct and disperse dye reached 92% and 87%, respectively. These results show the efficience of the evaluated processes for dye removal from textile wastewaters.

Genotoxic, mutagenic and cytotoxic effects of the commercial dye CI Disperse Blue 291 in the human hepatic cell line HepG2

Textile dyes are discarded into the aquatic ecosystem via industrial effluents and potentially expose humans and local biota to adverse effects. The commercial dye CI Disperse Blue 291 which contains the aminoazobenzene 2-[(2-bromo-4,6-dinitrophenyl)azo]-5(diethylamino)-4-methoxyacetanilide (CAS registry no. 56548-64-2), was tested for genotoxicity and cytotoxicity in the human hepatoma cell line HepG2, using the comet assay, micronucleus (MN) test and a cell viability test. Five different concentrations of the test compound were examined: 200 mu g/ml, 400 mu g/ml, 600 mu g/ml, 800 mu g/ml and 1000 mu g/ml. An increase in comet tail length and in the frequency of MN was detected with exposure of cells to concentrations of the commercial dye from 400 pg/ml. Furthermore, the dye was found to decrease cell viability. The results of this study demonstrate for the first time the genotoxic and mutagenic effects of the dye CI Disperse Blue 291 in mammalian cells, thus stressing the need to develop non-mutagenic dyes and to invest in improving the treatment of effluents. These measures will help to prevent harmful effects that these compounds can have on humans and aquatic organisms that come in contact with them. (C) 2007 Elsevier Ltd. All rights reserved.

Electrochemical reduction of disperse orange 1 textile dye at a boron-doped diamond electrode

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Spectrophotometric Evaluation of the Behavior of Disperse Red 1 Dye in Aqueous Media and its Interaction with Calf Thymus ds-DNA

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Analyses of the genotoxic and mutagenic potential of the products formed after the biotransformation of the azo dye Disperse Red 1

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Disperse Red 1 (textile dye) induces cytotoxic and genotoxic effects in mouse germ cells

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Potential of a bacterial consortium to degrade azo dye Disperse Red 1 in a pilot scale anaerobic-aerobic reactor

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The azo dye Disperse Red 13 and its oxidation and reduction products showed mutagenic potential

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)