Isolation of transforming growth factor-beta 2 cDNA from a fish, Cyprinus carpio by RT-PCR

Transforming Growth Factors-beta (TGF-beta s) have been described in many vertebrate species of amphibians, aves and mammals. In this report we demonstrate the presence of TGF-beta 2 in pisces. TGF-beta 2 has been cloned from a fish, Cyrinus carpio, by RT-PCR using degenerate oligonucleotide primers. Sequence analysis of the amplified product and alignment of the deduced amino acid sequence with the human TGF-beta 2 amino acid sequence revealed 81% and 93% identity in the precursor and the mature regions, respectively. The northern blot analysis of fish heart RNA shows a major messenger RNA species of about 8.0 kb and two messages of very low abundance of about 5.0 kb and 4.0 kb. The identification of TGF-beta 2 isoform in Pisces and it's high degree of homology with the mammalian isoform suggests that among all TGF-beta isoforms, TGF-beta 2 is the most conserved during evolution. (C) 1997 Elsevier Science B.V.

Preliminary investigation on growth responses of Cyprinus carpio L. fed on locally formulated artificial diets

The growth rate and feed conversion ratios of the common carp, Cyprinus carpio were measured for five test diets in 14-day replicate laboratory studies. The young carp were fed with artificial test diets with crude protein contents ranging from 14.50 to 21.42 per cent. Within this range of feed characteristic optimum growth rates were obtained with diets containing 20.25 and 21.42 per cent crude protein. The study of the effect of varying ration levels showed that growth rates increased with increases of ration size, but the food conversion efficiency and protein efficiency ratios decreased markedly as ration size was increased

Investment prospects in the production of the common carp (Cyprinus carpio) fingerlings in freshwater ponds

Since the introduction of Common carp Cyprinus carpio in Oyo State (Nigeria) from Israel in 1964, various local breeding methods have been employed in carp rearing to improve the survival rate at all stages of development during breeding. The physico-chemical parameters of the ponds which were simultaneously investigated for carp rearing in this study includes temperature (t), dissolved oxygen (DO) and hydrogen-ion concentration (pH). However, high rates of water displacement in the breeding ponds were unfavourable to the development of zooplankton which play important role in the food web of C. carpio. The survival rates of 15.88-69.50% and 19.60-33.83% obtained for the egg-hatchling and hatchling-fingerling stages respectively were encouraging. A breeding performance of this magnitude was found to be viable, breaking even in the fourth year. However, an increase in size of this trial project would be more profitable and increase fingerling supply as well as provide employment opportunities. This study thereby provides some baseline information on some local techniques and progress in the propagation of C. carpio and scope for further improvement

A numerical investigation on the flow physics of koi carp (Cyprinus carpio koi) burst-and-coast swimming

Abstract—Burst-and-coast is the most common locomotion type in freely routine swimming of koi carps (Cyprinus carpio koi), which consists of a burst phase and a coast phase in each cycle and mostly leads to a straight-line trajectory. Combining with the tracking experiment, the flow physics of koi carp’s burst-andcoast swimming is investigated using a novel integrated CFD method solving the body-fluid interaction problem. The dynamical equations of a deforming body are formulated. Following that, the loose-coupled equations of the body dynamics and the fluid dynamics are numerically solved with the integrated method. The two burst modes, MT (Multiple Tail-beat) and HT (Half Tail-beat), which have been reported by the experiments, are investigated by numerical simulations in this paper. The body kinematics is predicted and the flow physics is visualized, which are in good agreement with the corresponding experiments. Furthermore, the optimization on the energy cost and several critical control mechanisms in burst-and-coast swimming of koi carps are explored, by varying the parameters in its selfpropelled swimming. In this paper, energetics is measured by the two mechanical quantities, total output power CP and Froude efficiency Fr. Results and discussion show that from the standpoint of mechanical energy, burst-and-coast swimming does not actually save energy comparing with steady swimming at the same average speed, in that frequently changing of speed leads to decrease of efficiency.

Saprolegnia on roach (Leuciscus rutilus) and carp (Cyprinus carpio) [Translation from: Acta zool.pathol.Antverpiensia 43 107-123, 1976]

There is, in nature, as well as in the aquarium, a parasitic disease known as 'mousse' and which attacks predominantly fish. It is caused by Phycomycete fungi, genus Saprolegnia. The fungus causes external lesions and covers the fish with a thick white layer from whence comes the name 'mousse', commonly attributed to the disease, for which the scientific name is Saprolegnia. This article provides an overview of Saprolegnia infections on fish in nature and aquaria and then discusses symptomology of Saprolegnia in the mirror caro and t roach in more detail.

Common carp (Cyprinus carpio) breeding in Nigeria

The development of the common carp (Cyprinus carpio) breeding programs in Nigeria is discussed.

Stock evaluation and development of a breeding program for common carp (Cyprinus carpio) in Karnataka, India: progress of a research project

Common carp (Cyprinus carpio) is the single most important species for aquaculture in the state of Karnataka, India, where it is generally grown in polyculture with Indian major carps. Precocious maturation and unwanted reproduction in the species have been identified as constraints to increase production in aquaculture and culture-based fisheries in Karnataka state. Stocks of C. carpio obtained from Hungary (Amur and P3), Indonesia (Rajdanu) and Vietnam (SV) are being assessed alongside two local stocks (L-BRP and L-FRS) in a series of culture performance trials with the objective of setting up a base population for selective breeding. The paper presents progress of research being undertaken at the Fisheries Research Station, University of Agricultural Sciences, Bangalore, India.

Stocking of eggs versus hatchlings in rice fields: a comparison of survival and growth of common carp (Cyprinus carpio) fingerlings

The study assesses the relative profitability of stocking eggs versus hatchlings of common carp (Cyprinus carpio) in the rice-fish systems in Bangladesh. Results showed that although stocking eggs-covered water hyacinths directly into rice fields is a simple low cost option, the yields and profits are much higher from incubating eggs in cloth hapas and nursing hatchlings before stocking them into rice fields.

Progress toward a global genealogy of common carp (Cyprinus carpio L.) strains using mitochondrial nucleotide sequences data

As part of a study of genetic variation in the Vietnamese strains of the common carp (Cyprinus carpio L.) using direct DNA sequencing of mitochondrial control and ATPase6/8 gene regions, samples from a number of other countries were analyzed for comparison. Results show that the levels of sequence divergence in common carp is low on a global scale, with the Asian carp having the highest diversity while Koi and European carp are invariant. A genealogical analysis supports a close relationship among Vietnamese, Koi, Chinese Color and, to a lesser extent, European carp. Koi carp appear to have originated from a strain of Chinese red carp. There is considerable scope to extend this research through the analysis of additional samples of carp from around the world, especially from China, in order to generate a comprehensive global genealogy of common carp strains.

Influence of music on the growth of koi carp, Cyprinus carpio (Pisces: Cyprindae)

An experiment was carried out to investigate the influence of music on the growth of Koi Carp (Cyprinus carpio) by subjecting the fish to music. Weekly growth in weight was recorded and used to calculate the growth rate and specific growth rate. The difference in growth between the control and experiment groups of fishes was statistically tested for significance. It was observed that the growth of fish subjected to music was significantly higher.

Effect of sodium bicarbonate on hatching and on postembryonic growth of Brachydanio rerio, Cyprinus carpio, Labeo rohita, Cirrhinus mrigala and Catla catla

The treatment of sodium bicarbonate at the dosage of 100 to 1000 mg/litre has been tested experimentally on the hatching and postembryonic growth of Brachydanio rerio, Cyprinus carpio, Labeo rohita, Cirrhinus mrigala and Catla catla. Lower dosages of 100 to 200 mg have been found to reduce the hatching period, yolk absorption period and improve the survival and growth of the post embryonic larvae. The higher dosages of 500 to 1000 mg has been found to reduce the hatching percentage and post embryonic growth of the larvae. The findings indicate that application of sodium bicarbonate in the hatchery and nursery management of carps in the lower dosages of 100 to 200 mg/L to improve hatching and postembryonic growth.

The bacterial flora and their possible association with spoilage in a variety of fresh-water fish: Cyprinus carpio. var. communis

The bacteria from a variety of fresh-water fish, Cyprinus carpio. var. communis, showed the presence of micrococci, Gram positive and Gram negative rods. These have been characterized as far as was possible. Of thirty-eight strains of bacteria used, only six strains were considered as causing spoilage of fish flesh in experiments where flesh was incubated with individual cultures of the bacteria. These six strains had been found on the surface and/or intestine of the fish and support the suggestions that, after death, invasion of flesh by bacteria from the surface and intestine could be the cause of bacterial spoilage of fish.

Studies on growth and mortality of Cyprinus carpio (Lin.) in floating ponds

Growth and mortality rate of Cyprinus carpio (Linnaeus) under five different dietary conditions were studied in fifteen floating net cages in ponds of the Bangladesh Agricultural University Campus, Mymensingh. Growth rate was found to vary under different dietary conditions. The feed with mixture of 25% rice bran, 5% wheat bran, 30% linseed oil cake and 40% water hyacinth leaf meal exhibited the highest growth rate. The gain of log of body weight per unit increase of log of total length was significant. Significant survivals of the fishes were found.

Evaluation on propagation of common carp (Cyprinus carpio Lin.) with hormonal and natural stimuli

The success of breeding of common carp (Cyprinus carpio) using hormonal inducement and environmental stimuli was evaluated considering different sex ratios, and natural and artificial substrates. A total of 18 females (weighing 250 to 2200g) divided into 6 treatments were investigated. A successful spawning was observed in all the treatment groups, only. 66.66% female responded successfully to LHRH-A combined with dompheridone and 83.33% female in natural stimuli. Females induced with LHRH-A and dompheridone found prompt ovulation than that of natural stimulation. A significant variation (F=7.45, P<0.05) was found among the different treatment groups. The number of eggs released appear to depend on body weight (t=15.72, P<0.05), sex ratio (t=7.96, P<0.05) and percentage of ovulated females (t=5.34, P<0.05). Although environmentally stimulated females released more eggs than injected female (t=5.18, P<0.05) but their survival rate was similar (t=1.77, P<0.05). Comparison between the two approaches under the conditions of AIT hatchery shown that both are suitable for spawning induction in common carp. However, environmental stimulation is advantageous because of the less labor and lower cost required for ovulation.

Binding of radio-iodinated gonadotropin to the ovary of exotic carp (Cyprinus carpio Lin.)

Heterologous murrel gonadotropic hormone (m-GtH) binds to common carp oocyte plasma membrane and enhances steroid secretion. With increasing concentration of radio-labelled hormone the receptor binding is also found to increase linearly up to a certain concentration and then decrease. The [¹²⁵I] murrel GtH binding characteristics to a preparation of common carp ovarian plasma membrane shows saturability with high affinity. Scat chard plot analysis gave dissociation constant (Kd) of 0.81 X 10(super -9) M and maximum binding capacity (MBC) of 22.05 f mole/mg protein.