La construcción de un lugar de memoria en la Provincia de Buenos Aires : Mansión Seré, Morón, 1983-2007

En este trabajo reconstruimos sintéticamente el proceso de constitución de Mansión Seré como un lugar de memoria (Nora, 1984). Durante los primeros años de la última dictadura militar en Argentina (1976-1983), esta casona funcionó como centro de tortura y desaparición de personas. En el año 2000, el Estado local junto a organismos de Derechos Humanos decidieron convertirla en un espacio de rememoración y transmisión de lo ocurrido bajo el terrorismo de Estado. A partir de documentos y entrevistas, reconstruimos y analizamos los antecedentes político-institucionales del desarrollo de una política de la memoria y el proceso inicial de elaboración de un programa de memoria en este sitio

La construcción de un lugar de memoria en la Provincia de Buenos Aires : Mansión Seré, Morón, 1983-2007

En este trabajo reconstruimos sintéticamente el proceso de constitución de Mansión Seré como un lugar de memoria (Nora, 1984). Durante los primeros años de la última dictadura militar en Argentina (1976-1983), esta casona funcionó como centro de tortura y desaparición de personas. En el año 2000, el Estado local junto a organismos de Derechos Humanos decidieron convertirla en un espacio de rememoración y transmisión de lo ocurrido bajo el terrorismo de Estado. A partir de documentos y entrevistas, reconstruimos y analizamos los antecedentes político-institucionales del desarrollo de una política de la memoria y el proceso inicial de elaboración de un programa de memoria en este sitio

La construcción de un lugar de memoria en la Provincia de Buenos Aires : Mansión Seré, Morón, 1983-2007

En este trabajo reconstruimos sintéticamente el proceso de constitución de Mansión Seré como un lugar de memoria (Nora, 1984). Durante los primeros años de la última dictadura militar en Argentina (1976-1983), esta casona funcionó como centro de tortura y desaparición de personas. En el año 2000, el Estado local junto a organismos de Derechos Humanos decidieron convertirla en un espacio de rememoración y transmisión de lo ocurrido bajo el terrorismo de Estado. A partir de documentos y entrevistas, reconstruimos y analizamos los antecedentes político-institucionales del desarrollo de una política de la memoria y el proceso inicial de elaboración de un programa de memoria en este sitio

La construcción de un lugar de memoria en la Provincia de Buenos Aires : Mansión Seré, Morón, 1983-2007

En este trabajo reconstruimos sintéticamente el proceso de constitución de Mansión Seré como un lugar de memoria (Nora, 1984). Durante los primeros años de la última dictadura militar en Argentina (1976-1983), esta casona funcionó como centro de tortura y desaparición de personas. En el año 2000, el Estado local junto a organismos de Derechos Humanos decidieron convertirla en un espacio de rememoración y transmisión de lo ocurrido bajo el terrorismo de Estado. A partir de documentos y entrevistas, reconstruimos y analizamos los antecedentes político-institucionales del desarrollo de una política de la memoria y el proceso inicial de elaboración de un programa de memoria en este sitio

Chemical probes into the active centre of a heme thiolate monoxygenase

Linalool-8-monoxygenase, a typical bacterial P-450 heme thiolase, shows a high degree of substrate specificity towards linalool. The active site of the pure enzyme has been probed with a large number of substrate analogues with systematic alterations or conformational variations in the linalool molecule. The comparison of three parameters, the mo→mos conversion of the enzyme as a result of substrate binding monitored at 392 nm, theK D of the analogues giving information about energies of association and the relative turnover as substrate have given information about the space-filling characteristics of the substrates in the enzyme cleft, the number of contacts the molecules make with the respective domains of the enzyme and the distance of the site undergoing hydroxylation from the oxygen site, respectively. The data permit the conclusion that linalool makes contact with the enzyme by hydrogen bonding with the hydroxyl group as well through hydrophobic association with all the eight carbons carrying hydrogen in the molecules.

Sterochemical studies on cyclic peptides-VIII. Conformational analysis of hydrogen bonded cyclohexaglycyl molecule with a centre of inversion symmetry

A study on the conformational aspects of cyclo-hexaglycyl having inversion symmetry has been made. The cyclic backbone has been assumed to have two internal 4→1 types of NH... O hydrogen bonds. This molecule has been found to take up two types of conformations designated asA* andB* having nearly the same energy values. The theoretical conformations have been compared with the conformations of cyclohexaglycyl hemihydrate observed in the crystal structure. Two molecules with an approximate inversion symmetry are close to the conformation of the typeB* and two other molecules with exact inversino symmetry correspond nearly to the typesB* andA*. comparison with the theoretically possible conformations of cyclohexaglycyl molecule with 2-fold symmetry has been made. The preference of inversion symmetry and preferred ranges ofψ for glycyl molecules is discussed.

Optimal location of centre of gravity for swashplateless helicopter UAV and MAV

To investigate the use of centre of gravity location on reducing cyclic pitch control for helicopter UAV's (unmanned air vehicles) and MAV's (micro air vehicles). Low cyclic pitch is a necessity to implement the swashplateless rotor concept using trailing edge flaps or active twist using current generation low authority piezoceramic actuators. Design/methodology/approach – An aeroelastic analysis of the helicopter rotor with elastic blades is used to perform parametric and sensitivity studies of the effects of longitudinal and lateral center of gravity (cg) movements on the main rotor cyclic pitch. An optimization approach is then used to find cg locations which reduce the cyclic pitch at a given forward speed. Findings – It is found that the longitudinal cyclic pitch and lateral cyclic pitch can be driven to zero at a given forward speed by shifting the cg forward and to the port side, respectively. There also exist pairs of numbers for the longitudinal and lateral cg locations which drive both the cyclic pitch components to zero at a given forward speed. Based on these results, a compromise optimal cg location is obtained such that the cyclic pitch is bounded within ±5° for a BO105 helicopter rotor. Originality/value – The reduction in the cyclic pitch due to helicopter cg location is found to significantly reduce the maximum magnitudes of the control angles in flight, facilitating the swashplateless rotor concept. In addition, the existence of cg locations which drive the cyclic pitches to zero allows for the use of active cg movement as a way to replace the cyclic pitch control for helicopter MAV's.

Mutational analysis of the active centre of coronavirus 3C-like proteases.

Formation of the coronavirus replication-transcription complex involves the synthesis of large polyprotein precursors that are extensively processed by virus-encoded cysteine proteases. In this study, the coding sequence of the feline infectious peritonitis virus (FIPV) main protease, 3CL(pro), was determined. Comparative sequence analyses revealed that FIPV 3CL(pro) and other coronavirus main proteases are related most closely to the 3C-like proteases of potyviruses. The predicted active centre of the coronavirus enzymes has accepted unique replacements that were probed by extensive mutational analysis. The wild-type FIPV 3CL(pro) domain and 25 mutants were expressed in Escherichia coli and tested for proteolytic activity in a peptide-based assay. The data strongly suggest that, first, the FIPV 3CL(pro) catalytic system employs His(41) and Cys(144) as the principal catalytic residues. Second, the amino acids Tyr(160) and His(162), which are part of the conserved sequence signature Tyr(160)-Met(161)-His(162) and are believed to be involved in substrate recognition, were found to be indispensable for proteolytic activity. Third, replacements of Gly(83) and Asn(64), which were candidates to occupy the position spatially equivalent to that of the catalytic Asp residue of chymotrypsin-like proteases, resulted in proteolytically active proteins. Surprisingly, some of the Asn(64) mutants even exhibited strongly increased activities. Similar results were obtained for human coronavirus (HCoV) 3CL(pro) mutants in which the equivalent Asn residue (HCoV 3CL(pro) Asn(64)) was substituted. These data lead us to conclude that both the catalytic systems and substrate-binding pockets of coronavirus main proteases differ from those of other RNA virus 3C and 3C-like proteases.