Avaliação do efeito do composto tipo heparina isolado do caranguejo Chaceon fenneri na hemostasia e na morte celular

Heparin is a pharmaceutical animal widely used in medicine due to its potent anticoagulant effect. Furthermore, it has the ability to inhibit the proliferation, invasion and adhesion of cancer cells to vascular endothelium. However, its clinical applicability can be compromised by side effects such as bleeding. Thus, the search for natural compounds with low bleeding risk and possible therapeutic applicability has been targeted by several research groups. From this perspective, this study aims to evaluate the hemorrhagic and anticoagulant activities and citotoxic effect for different tumor cell lines (HeLa, B16-F10, HepG2, HS-5,) and fibroblast cells (3T3) of the Heparin-like from the crab Chaceon fenneri (HEP-like). The HEP-like was purified after proteolysis, ion-exchange chromatography, fractionation with acetone and characterized by electrophoresis (agarose gel) and enzymatic degradation. Hep-like showed eletroforetic behavior similar to mammalian heparin, and high trisulfated /Nacetylated disaccharides ratio. In addition, HEP-like presented low in vitro anticoagulant activity using aPTT and a minor hemorrhagic effect when compared to mammalian heparin. Furthermore, the HEP-like showed significant cytotoxic effect (p<0.001) on HeLa, HepG2 and B16-F10 tumor cells with IC50 values of 1000 ug/mL, after incubation for 72 hours. To assess the influence of heparin-like on the cell cycle in HeLa cells, analysis was performed by flow cytometry. The results of this analysis showed that HEP-like influence on the cell cycle increasing S phase and decreasing phase G2. Thus, these properties of HEP-like make these compounds potential therapeutic agents

Contribution to the biology and fishery of the deep-water red crab, Chaceon Affinis (A. Milne-Edwards & Bouvier, 1894) (Decapoda, Brachyura, Geryonidae) in deep waters of the Canary Islands (Central-East Atlantic)

[EN] This crab was captured in the whole range of depths sampled, although its highest abundance was found between 600 and 800 m, on muddy-rocky bottoms. Moreover, significant differences were observed in the average weight and length, according to depth of capture, island of origin, and date of survey. In general, the b parameter of length-weight relationship indicates a negative allometric growth pattern, although in some cases it was not statistically different from isometry, particularly in males. Males were heavier, larger, and more abundant in catches than females.

Avaliação do efeito do composto tipo heparina isolado do caranguejo Chaceon fenneri na hemostasia e na morte celular

Heparin is a pharmaceutical animal widely used in medicine due to its potent anticoagulant effect. Furthermore, it has the ability to inhibit the proliferation, invasion and adhesion of cancer cells to vascular endothelium. However, its clinical applicability can be compromised by side effects such as bleeding. Thus, the search for natural compounds with low bleeding risk and possible therapeutic applicability has been targeted by several research groups. From this perspective, this study aims to evaluate the hemorrhagic and anticoagulant activities and citotoxic effect for different tumor cell lines (HeLa, B16-F10, HepG2, HS-5,) and fibroblast cells (3T3) of the Heparin-like from the crab Chaceon fenneri (HEP-like). The HEP-like was purified after proteolysis, ion-exchange chromatography, fractionation with acetone and characterized by electrophoresis (agarose gel) and enzymatic degradation. Hep-like showed eletroforetic behavior similar to mammalian heparin, and high trisulfated /Nacetylated disaccharides ratio. In addition, HEP-like presented low in vitro anticoagulant activity using aPTT and a minor hemorrhagic effect when compared to mammalian heparin. Furthermore, the HEP-like showed significant cytotoxic effect (p<0.001) on HeLa, HepG2 and B16-F10 tumor cells with IC50 values of 1000 ug/mL, after incubation for 72 hours. To assess the influence of heparin-like on the cell cycle in HeLa cells, analysis was performed by flow cytometry. The results of this analysis showed that HEP-like influence on the cell cycle increasing S phase and decreasing phase G2. Thus, these properties of HEP-like make these compounds potential therapeutic agents

Efecto de la inclusión de harinas de cangrejo de diferente origen en piensos para bocinegro (Pagrus pagrus) sobre la textura, oxidación y composición del filete

[EN]The inclusion of two different crab meals in diets on fillet quality parameters was investigated in a six month growth trial with red porgy (Pagus pagrus). A high quality fish meal and fish oil diet was used as a control (Diet C). Fish meal protein in the control diet was replaced by increasing levels of protein from a river crab meal (Procamburus clarkii) (CR) and a marine crab meal (Chaceon affinis) (CM) at 10% and 20% each of them. The inclusion of both crab meals in diets, either at 10% and 20% substitution levels, did not affect the texture quality parameters of flesh except for the adhesiveness, where animal fed on CR20 showed the smallest value respect to those fed the Diet C. Compared to the control fish, a reduction of the fillet lipid oxidation indicated by the Tbars index was observed for fish fed both crab meal based diets, at the higher inclusion level (20%). Increasing dietary levels of the marine crab meal showed an increment of the monoenoicos, n-9 and oleic fatty acid content in the fillets. Results indicate that both crab meals used in present study are suitable as alternative ingredients for red porgy diets in terms of fish flesh quality.

Efecto de la inclusión de harinas de cangrejo de diferente origen en piensos para bocinegro (Pagrus pagrus) sobre el crecimiento, utilización de la dieta y coloración de la piel

[EN] The effect of dietary inclusion of two type of crab meal on growth, feed utilization and skin coloration performance was investigated in a growth trial with red porgy (Pagrus pagrus) of 233g initial body weight, during 6 month feeding period. High quality fish meal and fish oil diet was used as a control (Diet C). Protein of fish meal in the control was replaced by increasing dietary levels of protein derived from a river crab meal (Procamburus clarkii) (CR) and a marine crab meal (Chaceon affinis) (CM) at 10% and 20% each of them. Regarding growth results, fish fed the CM20 diet showed the highest values in absolute final weight and percent of the initial weight. For animals fed the crab meal based diets, the colour result was better than that of fish fed the control one, showing skin redness similar to that of the wild specimens. For both ingredients, increasing dietary inclusions were accompanied for an increment of the colour saturation, being in this case the obtained value for the CR meal higher than those for the CM meal. Present results indicate that crab meals used in this study are suitable as partial replacers for fish meal in diets for the red porgy.