Atividade viral do vírus respiratório sincicial bovino (brsv) em bezerros leiteiros

The purpose of the present study was to evaluate the antimicrobial activity of propolis extracts diluted in different solvents against bacteria from Staphylococcus genus. The study was performed in the Immunology and Microbiology Laboratory from Universidade Federal do Vale do São Francisco. The propolis extracts were prepared using brown propolis diluted in different solvents such as chloroform, methanol, ethyl acetate and grain alcohol. In order to determine the antimicrobial potential of extracts, agar well diffusion method was used, with controls for each diluent. After that, Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) methods were used. All tests were performed in triplicate. In the agar well diffusion test, the measurements of the inhibition zone for propolis extract were as follows: grain alcohol and propolis (2.88mm), methanol and propolis (2.41mm), chloroform and propolis (2.40mm) and ethyl acetate and propolis (0.83mm). The MBC of propolis extracts in different solvents were 93.75 μg/mL for grain alcohol, 375 μg/mL for chloroform and methanol and 3,000 μg/ml for ethyl acetate. Statistically significant differences were achieved comparing the inhibition zones of propolis diluted in grain alcohol and ethyl acetate (2.88 and 0.83 mm, respectively). Considering the low cost of therapy and the activity of the propolis against caprine mastitis pathogens, other studies regarding in vivo activity and chemical characterization are necessary, in addition to evaluation of the toxicological aspects of propolis extracts.

produção de mudas de maracujazeiro amarelo submetidas à salinidade em solo com biofertilizante bovino

The experiment was conducted in a greenhouse from May 1 to July 31, 2008, in Areia county, Paraiba State, PB, Brazil, in order to evaluate the effects of irrigation water salinity on initial growth of the passionfruit seedlings in non-saline substrate with and without bovine biofertilizer. The treatments were distributed in a completely randomized design, with three replications and twelve plants per plots, in a factorial arrangement 5 × 2 × 2, corresponding the former to the levels of salinity in the irrigation water: 0.5; 1.0; 2.0; 3.0 and 4.0 dS m-1, in soils with and without bovine biofertilizer applied at two moments (25 and 65 days after seedling emergence). The growth of the seedlings and the soil electrical conductivity were evaluated at the end of the experiment. The biofertilizer was diluted in a low saline water at a 1:1 ratio and was applied once two days before sowing, corresponding to 10% of the substrates volume. The increase in water salinity inhibited the growth in height of plants, leaf area and root length, but always to a lesser extent in the treatments with bovine biofertilizer. The increase in electrical conductivity of the irrigation water elevated the soil salinity, independently of the addition of biofertilizer.

Proteômica para investigar a congelabilidade do semen bovino

The present study was undertaken the protein composition in 2D-electrophoretic pattern (2DE) of the seminal plasma (SP) can interfere in the semen bull freezability, and if we can use that for predicting semen bull freezability. Samples were obtained of 20 bulls (different breeds) with a minimum of 3 years history semen production in commercial semen collection Center. All animals ranged between 2 - 7 years of age. The semen freezability was calculated by # of thawed and approved ejaculates / # of ejaculates submitted to cryopreservation (after semen evaluation and approved to submitted to freeze). The bulls were divided in 3 groups: HIGH (=>80% ejaculates approved); MEDIUM (>60% and <79% ejaculates approved); LOW (=<59% ejaculates approved); the pattern and criteria were the same used in the routine of the commercial semen Center. 68 gels were carried through by 2DE of SP samples indicated 225 detected spots with protein different amount (VION) comparing. Comparing bull ́s semen freezability and VION of each spot found difference among 2 spots from High and Low, even considering just spots with % of detection frequency bigger than 75%. The taurine bulls demonstrated more homogeneous profile when comparing with zebu bulls, considering number and frequency of appearance of spots. The results showed that proteomics can be a useful tool to predict the semen freezability, but we ́ll need to study better the interactions between sperm membrane, seminal plasma and extender to comprehend better which proteome phenotype interfere positive or negatively in the semen freezability.

Atividade antimicrobiana in vitro de extrato de Punica granatum L. sobre staphylococcus aureus isolado em leite bovino

Bovine mastitis is considered important disease causing major economic losses in dairy herds. Antimicrobial drug can promote resistance, chemical residues in food and environmental contamination. The purpose of this study was to evaluate the antibacterial activity in vitro of pomegranate extract on Staphylococcus aureus isolated from bovine milk, evaluate its antioxidant activity, and quantify levels of total phenols and fravonoides of the different extracts used. Aqueous extracts were used in nature and dry, from the peel of the fruit (EAC) and leaves (EAF). Additionally, it was evaluated the antioxidant activity (AA%), total phenols and flavonoids. Milk samples were inoculated, incubated, and the colonies were characterized as Staphylococcus aureus were adjusted to 1.0x106 UFC/mL in the 6 standard of the MacFarland scale. The sensitivity of the microbial isolates were determined in quintuplicate, in disk diffusion test. The minimum inhibitory concentration was determined by visible inhibition zone greater than 15mm. The results were evaluated by ANOVA, Tukey 5%, using the program SISVAR 5.3 - DEX/UFLA. Results implied that the aqueous extract from the bark of dried fruit was capable of inhibiting bacterial growth at concentrations of 3%. The other treatments only showed this activity, from the concentrations of 15%, 20% and 30% for dry EAF, in nature EAC and in nature EAF, respectively. Regarding the action antioxidant of dry EAC, was not correlated with total phenols and flavonoids. Probably other alkaloids substances present in the extract studied, may have been responsible for this activity. It is concluded that extracts of Punica granatum L., especially those obtained by the shell of the fruit, showed inhibitory activity against S. aureus, indicating your use potential for the control of bovine mastitis.

Crescimento inicial e consumo hídrico de nim submetido ao estresse salino e biofertilizante bovino

An experiment was carried out during the period of January to July/2010, in municipality of Areia, Paraiba State, Brazil, in order to evaluate effects of the irrigation with saline water, bovine biofertilizer and drainage of the soil on water consumption and growth of neem seedlings. The experimental design was in randomized blocks using factorial 5×2×2, referring to five levels of saline water (0.5; 1.5; 3.0; 4.5; 6.0 dS nr-1) in soil without and with bovine biofertilizer and in pots without and with drainage. In plants the water consumption, growth in height, stem diameter, number of leaves, dry matter of roots, aerial part and total dry mass were evaluated and in soil the electrical conductivity of saturation extract - EC was determined. The bovine biofertilizer, after dilution in non saline water (0.49 dS nr-1) e no chlorinated water in 1:1 ratio was applied once two days before sowing, equivalent to 10% of substrate volume. Irrigation was applied daily with each water type applying volume sufficient to maintain the soil with water content at level of field capacity. From results the increase in salinity of water inhibited the water consumption by plants independently of the soil with or without bovine biofertilizer. The salinity of water in soil with and without bovine biofertilizer also reduced the growth of neem plants but with more pronounced effect in the treatments without application of organic fertilizer to soil in liquid form.

Comportamento do noni à salinidade da água de irrigação em solo com biofertilizante bovino

Salt excess in soil and water used for irrigation can cause significant loss of production and growth in cultivated plants. Among some options for reduction of negative effects of salts to plants in cultivated areas, fermented bio fertilizer has been used to grow vegetables and fruit tree irrigated with saline water. The study aimed at evaluating the behavior of the noni plant to salinity of the irrigation water in substrate with and with no bio fertilizer. Treatments were arranged in a randomized block design with four replications, using a 5 × 2 factorial arrangement. Five levels of electrical conductivity of irrigation water (0.5, 1.5, 3.0, 4.5, 6.0 dS m-1) were used in substrates with and with no bio fertilizer. Parameters were evaluated as follows: plant height, stem diameter, number of leaves, leaf area, shoot dry matter and water consumption. All evaluated variables were negatively affected by the increase in salt concentration of the irrigation water, but always with less intense effects in treatments with bio fertilizer.The bio fertilizer does not eliminate, but mitigates the negative effects of salts in noni plants.

Propriedade antimicrobiana de óleos essenciais de plantas condimentares com potencial de uso como conservante em carne e hambúrguer bovino e testes de aceitação

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Estudo da resposta de cultura utilizando-se soro bovino fetal e soro de plasma rico em plaquetas

Pós-graduação em Pesquisa e Desenvolvimento (Biotecnologia Médica) - FMB

Avaliação dos efeitos da exposição experimental ao herpesvírus bovino tipo 5 em embriões bovinos produzidos in vitro

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Esterco de bovino e calcário para formação de mudas de guanandi

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Efeitos da aplicação de salmoura com plasma bovino em biceps femoris (coxão duro) injetado, cru e cozido na estabilidade física, química, microbiológica e sensorial

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Implantes do sistema T. F. (Tissue functional) em alvéolos imediatamente após exodontia com e sem membrana de osso bovino: estudo histológico em cães

Pós-graduação em Odontologia - FOA

Estudo comparativo entre peritônio bovino e biomembrana de látex natural na substituição de fragmento da parede da bexiga em coelhos (Oryctolagus cuniculus)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Influência das proteínas morfogenéticas ósseas adsorvidas à hidroxiapatita e aglutinantes do colágeno bovino na consolidação da fratura do rádio em coelhos

Pós-graduação em Medicina Veterinária - FMVZ

Avaliação histopatológica, imunoistoquímica e detecção molecular do DNA viral no sistema nervoso central de bovinos inoculados experimentalmente com herpesvirus bovino 5

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)