959 resultados para Birch, Robert, 1808-1842.


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Originally published in the Federal Republican of Baltimore. Printed by Benjamin Edes

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Pós-graduação em Letras - FCLAS

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Albicidin phytotoxins are pathogenicity factors in a devastating disease of sugarcane known as leaf scald, caused by Xanthomonas albilineans. A gene (albD) from Pantoea dispersa has been cloned and sequenced and been shown to code for a peptide of 235 amino acids that detoxifies albicidin. The gene shows no significant homology at the DNA or protein level to any known sequence, but the gene product contains a GxSxG motif that is conserved in serine hydrolases. The AlbD protein, purified to homogeneity by means of a glutathione S-transferase gene fusion system, showed strong esterase activity on p-nitrophenyl butyrate and released hydrophilic products during detoxification of albicidins. AlbD hydrolysis of p-nitrophenyl butyrate and detoxification of albicidins required no complex cofactors. Both processes were strongly inhibited by phenylmethylsulfonyl fluoride, a serine enzyme inhibitor. These data strongly suggest that AlbD is an albicidin hydrolase. The enzyme detoxifies albicidins efficiently over a pH range from 5.8 to 8.0, with a broad temperature optimum from 15 to 35°C. Expression of albD in transformed X. albilineans strains abolished the capacity to release albicidin toxins and to incite disease symptoms in sugarcane. The gene is a promising candidate for transfer into sugarcane to confer a form of disease resistance.

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pt.15 (1853) [Nomenclature of Diptera.I]

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Mode of access: Internet.

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Mode of access: Internet.

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El Pelayo (ensayo épico) -- Poesías liricas -- El estudiante de Salamanca (cuento) -- El diablo mundo (poema)

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Ed. by José Garcia de Villalta.

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Vol. 1 accompanies "Verzeichniss der öffentlichen und Privat-Vorlesungen, welche am Hamburgischen Academischen Gymnasium von Ostern 1842 bis Ostern 1843 gehalten werden."

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Bound with: Indiana Historical society publications, vol 5, nos. 1-6.