Microsatellite loci for euglossine bees (Hymenoptera : Apidae)

The Neotropical Euglossini (Hymenoptera: Apidae) are important pollinators of many flowering plants, particularly orchids. Lack of highly polymorphic genetic markers for euglossine species has limited the study of their social organization and inbreeding. We therefore developed microsatellite markers for two species, Eulaema nigrita (11 loci) and Euglossa cordata (nine loci), most of which were highly polymorphic in the source species and in a range of related euglossine bees.

Microsatellite loci for the eusocial Lasioglossum malachurum and other sweat bees (Hymenoptera, Halictidae)

Sweat bees (family Halictidae) comprise a numerous and diverse group that are arguably among the most socially labile of all insect taxa. Given the lack of highly variable markers for eusocial species of the family, we developed a suite of dinucleotide and trinucleotide markers for one of its members, the Eurasian Lasioglossum malachurum, and used them to amplify DNA from other halictids. Loci were highly variable in L. malachurum and amplified DNA from many other halictids.

Ultrastructural features of the regenerative cells of the bees (Hymenoptera, Apidae) midguts

The epithelium of the bee ventriculus is formed by two cell types: the principal or digestive cells and the regenerative cells. In this article the ultrastructure of the regenerative cells is described, as well as the features of their differentiation into digestive cells during epithelium renewal.

Lipid distribution in salivary glands of larvae and adult bees (Hymenoptera : Apidae)

Cytochemical studies were carried out to establish lipid distribution in the salivary glands of larvae and adult bees, using the imidazole buffer technique. In the duct cells of the larval salivary gland, the reaction was positive in the epicuticle and negative in the glandular lumen. The absence of smooth endoplasmic reticulum and the presence of lipids in the intercellular space suggest that lipids absorbed from the haemolymph could be used in the constitution of the epicuticle, after having been conveyed through the epithelium. In adult workers (new-emerged, nurse and forager workers), the head salivary glands presented a positive reaction in the secretion in glandular lumen, identifying its lipidic nature.

Cephalic salivary glands of two species of advanced eusocial bees (Hymenoptera: Apidae): morphology and secretion

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Cephalic salivary gland ultrastructure of worker and queen eusocial bees (Hymenoptera, Apidae)

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Preliminar report on the presence of tegumentar glands in the thorax of Meliponinae bees (Hymenoptera, Apidae)

Este é um primeiro relato sobre a presença de células glandulares no tegumento lateral do mesotórax de abelhas da subfamília Meliponinae. Trata-se de células glandulares de classe III que estão presentes em rainhas, operárias e machos. As glândulas são mais desenvolvidas nas rainhas que nas operárias e nestas mais que nos machos.

Taxonomic diversity, niche width and similarity in the use of plant resources by bees (Hymenoptera: Anthophila) in a cerrado area

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Body size and flight distance in stingless bees (Hymenoptera: Meliponini): inference of flight range and possible ecological implications

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Comparative study of wax glands in four Meliponini bees (Hymenoptera, Apidae) producing different quantities of wax

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Composition and diversity of bees (Hymenoptera) attracted by Moericke traps in an agricultural area in Rio Claro, state of São Paulo, Brasil

Foram estudadas a composição e diversidade de abelhas em uma área agrícola no município de Rio Claro, Estado de São Paulo, de maio de 2003 a junho de 2004, utilizando armadilha de Moericke. O local de coleta, uma área com 58,08 hectares, caracteriza-se pela produção de grãos e a prática de plantio direto, sendo que 70% da área de entorno é utilizada para o plantio de cana-de-açúcar. Foram coletadas 456 abelhas distribuídas em 20 gêneros, pertencentes às famílias Andrenidae (4,8%), Apidae (40,8%) e Halictidae (54,4%). Espécimes dos gêneros Dialictus (38%) e Diadasia (30%) foram predominantes nesta área. A diversidade de espécies avaliadas pelos índices de Shannon e Simpson foram H =1,88 e 1/D= 4.15, respectivamente, e o índice de Equitatibilidade de 0,61.

Ovarian development in Meliponine bees (Hymenoptera: Apidae): the effect of queen presence and food on worker ovary development and egg production

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Comparative study of digestive cells in different midgut regions of stingless bees (Hymenoptera: Apidae: Meliponinae)

Ultrastructure of the digestive cells was analyzed in three midgut regions (anterior, middle and posterior) of stingless bees. Variations occurs in the presence of lipid inclusions in the cells from posterior midgut and presence of double-membraned vesicles associated to microvilli in the anterior midgut. However, basal plasmic membrane infoldings and augmentation of surface area achieved by microvilli ale very similar in all midgut regions. These results not supported the existence of fluid fluxes in the ectoperitrophic space and suggest that digestive cells in stingless bees are polifunctional, that is, there is not midgut region specialized in secretion ol absorption as observed in other insects.

THE EVOLUTIVE SIGNIFICANCE OF POLLEN USE AS PROTEIN RESOURCE BY TRIGONINI BEES (HYMENOPTERA, APIDAE, MELIPONINAE)

The origin of bees was marked by the change from the use of animal of vegetal protein to feed the larvae. The advantages of this change and the existence of some necrophagous species among the bees is discussed regarding to the poor digestibility of pollen.

A specialized midgut region in stingless bees (Hymenoptera: Apidae: Meliponinae)

Ultrastructure of the anterior end of the midgut of three Neotropical stingless bees, including an obligate necrophagous species Trigona hypogea, suggests that this midgut region has no role in nutrient absorption because its lumen is reduced by a thick cuticle that lines the outer epithelium of the cardiac valve. A possible involvement of this midgut region in the production of the peritrophic membrane compounds is discussed.