Effects of a lectin-like protein isolated from Acacia farnesiana seeds on phytopathogenic bacterial strains and root-knot nematode

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Purification of a PHA-Like chitin-binding protein from Acacia farnesiana seeds: A time-dependent oligomerization protein

A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO erythrocytes either native or treated with proteolytic enzymes. In sodium dodecyl sulfate gel electrophoresis under reducing and nonreducing conditions, AFAL separated into two bands with a subunit molecular mass of 35 and 50 kDa. The homogeneity of purified protein was confirmed by chromatofocusing with a pI=4.0+/-0.5. Molecular exclusion chromatography confirmed time-dependent oligomerization in AFAL, in accordance with mass spectrometry analysis, which confers an alteration in AFAL affinity for chitin. The protein sequence was obtained by a liquid chromatography quadrupole time-of-flight experiment and showed that AFAL has 68% and 63% sequence similarity with lectins of Phaseolus vulgaris and Dolichos biflorus, respectively.

RCS/linear discrete actuator study /

"AFAL-TR-88-039."

Design handbook for high temperature aerospace antennas.

"Technical report AFAL-TR-65-267"

Spanish Photography Yearbooks between the Autarchy and the Transition A Documentary Analysis (1958-1980)

The first yearbook on Spanish artistic photography was published in 1924 and following the Civil War of 1936-1939, Madrid’s Real Sociedad Fotográfica, through its Sombras magazine, published two others in 1946 and 1948. There would not be another publication until 1958, when the Agrupación Fotográfica Almeriense put out the third yearbook, during a period of economic recovery following the country’s admission to the U.N. at the end of 1955. However, it was not until 1972 when Spanish photography resumed the publication of annual summaries of the authors and their works. From then on until 1980, nine yearbooks were published by two institutions: The Consorcio del Libro Técnico (Cotec) and the Editorial Everest publishing company. The object of this study is to analyze the contents of these yearbooks in order to determine and furnish new data on the history of Spanish photography: authors, works, publications, national and international relations and the purpose of their publications as a means for supporting photography.