87 resultados para Adiantum capillus-veneris
Resumo:
Chloroplast movement was induced by partial cell illumination using a high-fluence blue microbeam in light-grown and dark-adapted prothallial cells of the fern Adiantum capillus-veneris. Chloroplasts inside the illuminated area moved out (high-fluence response [HFR]), whereas those outside moved toward the irradiated area (low-fluence response [LFR]), although they stopped moving when they reached the border. These results indicate that both HFR and LFR signals are generated by high-fluence blue light of the same area, and that an LFR signal can be transferred long-distance from the beam spot, although an HFR signal cannot. The lifetime of the HFR signal was calculated from the traces of chloroplast movement induced by a brief pulse from a high-fluence blue microbeam to be about 6 min. This is very short compared with that of the LFR (30–40 min; T. Kagawa, M. Wada [1994] J Plant Res 107: 389–398). These data indicate that the signal transduction pathways of the HFR and the LFR must be distinct.
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本研究中运用四种不用的方法提取海带孢子体RNA，通过对比分析，确定了采用CTAB提取液[2% (w/v) CTAB,100 mM Tris-HC1 (pH 8.0), 50 mM EDTA, pH 7.5, 2 M NaCl, 50 mM DTT]提取RNA的方法，该方法的主要改进之处： (1) 高浓度（50 mM）的 DTT作为防止多酚氧化的还原剂加入提取液中；(2) 1/4 体积的乙醇和 1/9 体积的3 M 醋酸钾（pH 4.8）用来沉淀去除多糖；(3) 1/10倍体积的3 mol/L醋酸钠（pH 5.0）和两倍体积的乙醇选择性沉淀RNA；(4) -80℃沉淀30 min沉淀RNA。CTAB改进法提取的海带孢子体RNA质量好（A260/280 =1.96±0.05)，产量高（68 μg/g），分离的RNA可用于RT-PCR反应，扩增相关基因片段。该方法提取海带配子体RNA效果也很好。 根据拟南芥(Arabidopsis thaliana)、 莱茵衣藻(Chlamydomonas reinhardtii) 、铁线蕨(Adiantum capillus-veneris)、转板藻(Mougeotia scalaris)和无隔藻(Vaucheria frigida)等的蓝光受体蛋白保守序列，设计一系列简并引物，进行RT-PCR扩增，筛选并克隆测序了4条序列片段，通过比对分析，所得序列与已知的蓝光受体基因序列不一致。在所获得的序列中，一条783 bp的序列与肌醇-1-磷酸合成酶基因的片段有较高的相似性，通过RACE法克隆出该基因的3’末端，5’末端克隆没有成功，全长序列有待深入分析。
Resumo:
A new species of Adiantum is described from California. This species is endemic to northern California and is currently known only from Shasta County. We describe its discovery after first being collected over a century ago and distinguish it from Adiantumjordanii and Adiantumcapillus-veneris. It is evergreen and is sometimes, but not always, associated with limestone. The range of Adiantumshastense Huiet & A.R.Sm., sp. nov., is similar to several other Shasta County endemics that occur in the mesic forests of the Eastern Klamath Range, close to Shasta Lake, on limestone and metasedimentary substrates.
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UANL
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AR
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AR
