Multimodal Highlighting of Structural Abnormalities in Diabetic Rat and Human Corneas.

PURPOSE: This study aimed to highlight structural corneal changes in a model of type 2 diabetes, using in vivo corneal confocal microscopy (CCM). The abnormalities were also characterized by transmission electron microscopy (TEM) and second harmonic generation (SHG) microscopy in rat and human corneas. METHODS: Goto-Kakizaki (GK) rats were observed at age 12 weeks (n = 3) and 1 year (n = 6), and compared to age-matched controls. After in vivo CCM examination, TEM and SHG microscopy were used to characterize the ultrastructure and the three-dimensional organization of the abnormalities. Human corneas from diabetic (n = 3) and nondiabetic (n = 3) patients were also included in the study. RESULTS: In the basal epithelium of GK rats, CCM revealed focal hyper-reflective areas, and histology showed proliferative cells with irregular basement membrane. In the anterior stroma, extracellular matrix modifications were detected by CCM and confirmed in histology. In the Descemet's membrane periphery of all the diabetic corneas, hyper-reflective deposits were highlighted using CCM and characterized as long-spacing collagen fibrils by TEM. SHG microscopy revealed these deposits with high contrast, allowing specific detection in diabetic human and rat corneas without preparation and characterization of their three-dimensional organization. CONCLUSION: Pathologic findings were observed early in the development of diabetes in GK rats. Similar abnormalities have been found in corneas from diabetic patients. TRANSLATIONAL RELEVANCE: This multidisciplinary study highlights diabetes-induced corneal abnormalities in an animal model, but also in diabetic donors. This could constitute a potential early marker for diagnosis of hyperglycemia-induced tissue changes.

Genetics and teratology : research programs of the National Institute of Child Health and Human Development.

"Prepared by the Genetics and Teratology Section of the Clinical Nutrition and Early Development Branch for presentation to the National Advisory Child Health and Human Development Council, May 1980"--P. 2 of cover.

The early diagnosis of congenital abnormalities /

Includes index.

Philosophie anatomique ... Par M. le Cher Geoffroy-Saint-Hilaire ...

1

Philosophie anatomique ... Par M. le Cher Geoffroy-Saint-Hilaire ...

2

Annual report /

Description based on: 17th (1922/23)

Occupational marks and other physical signs; a guide to personal identification.

Mode of access: Internet.

Die Morphologie der Missbildungen des Menschen und der Tiere.

Vol. 3: Suppl. edited by G. Gruber, 1960.

Die tierschen Missbildung in ihren Bezeihungen zur Experiment. Entwicklungsgeschichte (entwicklungsmechanik) und zur Phylogenie.

Separat-abdruck aus den Verhandlungen der Schweizer. Naturforschenden Gesellschaft in St. Gallen, 1906.

La tératogenèse: étude des variations de l'organisme.

Includes bibliography.

Adverse pregnancy outcomes reporting system, item-specific completeness of reporting for 1989.

Cover title.

Birth defects in Illinois : a report on mortality and county-specific prevalence at birth /

"June 1997."

Birth defects in Chicago and the state of Illinois, 1989-1993 /

"October 1994."

Birth defects and other adverse pregnancy outcomes in Illinois, 1998-2002 : a report on county-specific incidence.

" ... supported by grant/cooperative agreement number U50/CCU523303 from the U.S. Centers for Disease Control and Prevention."--Page ii.

Illinois adverse pregnancy outcomes reporting system : assessment and recommendations.

Project director: Manorama Khare.