Seminari d'aplicaci?? a l'aula de les TIC : CP S' Albufera, Port d'Alc??dia. 'Seminario de aplicaci??n en el aula de las TIC : CP S' Albufera, Port d'Alc??dia'

Se recoge la experiencia de un seminario realizado en el CP s'Albufera del Port d'Alc??dia, Mallorca, de iniciaci??n a las tecnolog??as de la informaci??n y la comunicaci??n. Se comenta la metodolog??a de trabajo seguida por el grupo de profesores y profesoras participantes para el vaciado de Cd-Rom educativos, explic??ndose los 35 elementos vaciados y los par??metros analizados.

L'educaci?? en el segle XIX a Alc??dia : de 1838 a 1899. 'La educaci??n en el siglo XIX en Alc??dia : de 1838 a 1899'

Resumen tomado del recurso

L'escola del P??sit de pescadors d'Alc??dia. 'La escuela del P??sito de pescadores de Alc??dia'

Se analiza la escuela del P??sito de pescadores de Alc??dia, instituci??n creada por los propios socios con la intenci??n de cubrir las carencias educativas, formativas y culturales existentes en la d??cada de los a??os 20 en el puerto de Alc??dia. De hecho esta escuela funcion?? hasta los a??os 60, aunque a partir de 1943 se nacionaliz?? con el nombre de 'Escola d'Orientaci?? Mar??tima i Pesquera'. Su creaci??n fue calurosamente recibida por la localidad, que ??nicamente contaba com una escuela nacional de ni??os, insuficiente para atender las necesidades escolares. En un principio se ubic?? en un edificio de la plaza de la Constituci??n, posteriormente se emplaz?? en la calle Mayor y al acabar la guerra civil se traslad?? al Puerto de Alcudia.La ense??anza se impart??a siguiendo los criterios de la escuela p??blica del Estado, utilizando los mismos libros y la misma metodolog??a y, como el resto de escuelas, estaba sujeta a las visitas del inspector de la provincia. La obra analiza tambi??n la adecuaci??n de la ense??anza al entorno marinero, el higienismo escolar y social que un??a la voluntad de desterrar el analfabetismo com el inter??s en la promoci??n social, profesional y econ??mica de toda la poblaci??n.

Con??ixer Alc??dia. 'Conocer Alc??dia'

Resumen tomado del propio recurso

El port d'Alc??dia, ahir i avui. 'El puerto de Alcudia, ayer y hoy'

Material destinado a dar a conocer la historia del puerto de la ciudad de Alcudia (Mallorca) y las principales actividades econ??micas que en ??l se realizan.

Ricardo Alc??ntara : el senyor de la Terra Mitjana. 'Ricardo Alc??ntara : el se??or de la Tierra Media'

Resumen de la revista en catal??n

Juegos tradicionales de la comarca de Alc??ntara

Se recopilan 30 juegos infantiles tradicionales de la comarca de Alc??ntar (C??ceres), clasificados en funci??n de los elementos que se utilizan para llevarlos a cabo (cuerda, pelota, etc.) y si estos se ejecutan con o sin materiales. Con la elaboraci??n de este trabajo se pretend??a poner en valor elementos de la cultura tradicional de la zona, establecer entre los alumnos una din??mica de investigaci??n como procedimiento did??ctico para la recuperaci??n del patrimonio oral y ofrecer a los docentes un material que puede extrapolarse a diversas ??reas (educaci??n f??sica, lenguaje, conocimiento del medio, etc.) y tem??ticas de transversalidad asociadas a cuestiones de g??nero. De cada juego se especifica el n??mero m??nimo de participantes, la edad de los mismos, los materiales que se requieren para llevarlo a cabo, una descripci??n de c??mo se desarrolla y de sus implicaciones did??cticas.

Viajando por Extremadura : parando en...Valencia de Alc??ntara

Se presenta un proyecto llevado a cabo en el CEIP General Navarro y Alonso de Celada (Valencia de Alc??ntara, C??ceres) cuyo objetivo era conocer m??s profundamente la regi??n y la localidad del centro, en concreto aspectos sobre la geograf??a, el arte, la cultura, personajes ilustres, folclore, etc.

Evaluación de un ALC entre EEUU y Chile

Incluye Bibliografía

Cooperación en política de competencia y acuerdos comerciales en América Latina y el Caribe (ALC)

Incluye Bibliografía

Development of a high-level gene expression system for the production of bioplastics in sugarcane

Despite various approaches, the production of biodegradable plastics such as polyhydroxybutyrate (PHB) in transgenic plants has met with limited success due largely to low expression levels. Even in the few instances where high levels of protein expression have been reported, the transgenic plants have been stunted indicating PHB is phytotoxic (Poirier 2002). This PhD describes the application of a novel virus-based gene expression technology, termed InPAct („In Plant Activation.), for the production of PHB in tobacco and sugarcane. InPAct is based on the rolling circle replication mechanism by which circular ssDNA viruses replicate and provides a system for controlled, high-level gene expression. Based on these features, InPAct was thought to represent an ideal system to enable the controlled, high-level expression of the three phb genes (phbA, phbB and phbC) required for PHB production in sugarcane at a preferred stage of plant growth. A Tobacco yellow dwarf virus (TbYDV)-based InPAct-phbA vector, as well as linear vectors constitutively expressing phbB and phbC were constructed and different combinations were used to transform tobacco leaf discs. A total of four, eight, three and three phenotypically normal tobacco lines were generated from discs transformed with InPAct-phbA, InPAct-phbA + p1300-TaBV P-phbB/phbC- 35S T, p1300-35S P-phbA-NOS T + p1300-TaBV P-phbB/phbC-35S T and InPAct-GUS, respectively. To determine whether the InPAct cassette could be activated in the presence of the TbYDV Rep, leaf samples from the eight InPActphbA + p1300-TaBV P-phbB/phbC-35S T plants were agroinfiltrated with p1300- TbYDV-Rep/RepA. Three days later, successful activation was indicated by the detection of episomes using both PCR and Southern analysis. Leaf discs from the eight InPAct-phbA + p1300-TaBV P-phbB/phbC-35S T transgenic plant lines were agroinfiltrated with p1300-TbYDV-Rep/RepA and leaf tissue was collected ten days post-infiltration and examined for the presence of PHB granules. Confocal microscopy and TEM revealed the presence of typical PHB granules in five of the eight lines, thus demonstrating the functionality of InPActbased PHB production in tobacco. However, analysis of leaf extracts by HPLC failed to detect the presence of PHB suggesting only very low level expression levels. Subsequent molecular analysis of three lines revealed low levels of correctly processed mRNA from the catalase intron contained within the InPAct cassette and also the presence of cryptic splice sites within the intron. In an attempt to increase expression levels, new InPAct-phb cassettes were generated in which the castorbean catalase intron was replaced with a synthetic intron (syntron). Further, in an attempt to both increase and better control Rep/RepA-mediated activation of InPAct cassettes, Rep/RepA expression was placed under the control of a stably integrated alc switch. Leaf discs from a transgenic tobacco line (Alc ML) containing 35S P-AlcR-AlcA P-Rep/RepA were supertransformed with InPAct-phbAsyn or InPAct-GUSsyn using Agrobacterium and three plants (lines) were regenerated for each construct. Analysis of the RNA processing of the InPAct-phbAsyn cassette revealed highly efficient and correct splicing of the syntron, thus supporting its inclusion within the InPAct system. To determine the efficiency of the alc switch to activate InPAct, leaf material from the three Alc ML + InPAct-phbAsyn lines was either agroinfiltrated with 35S P-Rep/RepA or treated with ethanol. Unexpectedly, episomes were detected not only in the infiltrated and ethanol treated samples, but also in non-treated samples. Subsequent analysis of transgenic Alc ML + InPAct-GUS lines, confirmed that the alc switch was leaky in tissue culture. Although this was shown to be reversible once plants were removed from the tissue culture environment, it made the regeneration of Alc ML + InPAct-phbsyn plant lines extremely difficult, due to unintentional Rep expression and therefore high levels of phb expression and phytotoxic PHB production. Two Alc ML + InPAct-phbAsyn + p1300-TaBV P-phbB/phbC-35S T transgenic lines were able to be regenerated, and these were acclimatised, alcohol-treated and analysed. Although episome formation was detected as late as 21 days post activation, no PHB was detected in the leaves of any plants using either microscopy or HPLC, suggesting the presence of a corrupt InPAct-phbA cassette in both lines. The final component of this thesis involved the application of both the alc switch and the InPAct systems to sugarcane in an attempt to produce PHB. Initial experiments using transgenic Alc ML + InPAct-GUS lines indicated that the alc system was not functional in sugarcane under the conditions tested. The functionality of the InPAct system, independent of the alc gene switch, was subsequently examined by bombarding the 35S Rep/RepA cassette into leaf and immature leaf whorl cells derived from InPAct-GUS transgenic sugarcane plants. No GUS expression was observed in leaf tissue, whereas weak and irregular GUS expression was observed in immature leaf whorl tissue derived from two InPAct- GUS lines and two InPAct-GUS + 35S P-AlcR-AlcA P-GUS lines. The most plausible reason to explain the inconsistent and low levels of GUS expression in leaf whorls is a combination of low numbers of sugarcane cells in the DNA replication-conducive S-phase and the irregular and random nature of sugarcane cells bombarded with Rep/RepA. This study details the first report to develop a TbYDV-based InPAct system under control of the alc switch to produce PHB in tobacco and sugarcane. Despite the inability to detect quantifiable levels of PHB levels in either tobacco or sugarcane, the findings of this study should nevertheless assist in the further development of both the InPAct system and the alc system, particularly for sugarcane and ultimately lead to an ethanol-inducible InPAct gene expression system for the production of bioplastics and other proteins of commercial value in plants.

The effect of post-match alcohol ingestion on recovery from competitive Rugby League matches

This study investigated the effects of alcohol ingestion on lower body strength and power, and physiological and cognitive recovery following competitive Rugby League matches. Nine male Rugby players participated in two matches, followed by one of two randomized interventions; a control or alcohol ingestion session. Four hours post-match, participants consumed either beverages containing a total of 1g of ethanol per kg bodyweight (vodka and orange juice; ALC) or a caloric and taste matched non-alcoholic beverage (orange juice; CONT). Pre, post, 2 h post and 16 h post match measures of countermovement jump (CMJ), maximal voluntary contraction(MVC), voluntary activation (VA), damage and stress markers of creatine kinase (CK), C-reactive protein (CRP), cortisol, and testosterone analysed from venous blood collection, and cognitive function (modified Stroop test) were determined. Alcohol resulted in large effects for decreased CMJ height(-2.35 ± 8.14 and -10.53 ± 8.36 % decrement for CONT and ALC respectively; P=0.15, d=1.40), without changes in MVC (P=0.52, d=0.70) or VA (P=0.15, d=0.69). Furthermore, alcohol resulted in a significant slowing of total time in a cognitive test (P=0.04, d=1.59), whilst exhibiting large effects for detriments in congruent reaction time (P=0.19, d=1.73). Despite large effects for increased cortisol following alcohol ingestion during recovery (P=0.28, d=1.44), post-match alcohol consumption did not unduly affect testosterone (P-0.96, d=0.10), CK (P=0.66, d=0.70) or CRP(P=0.75, d=0.60). It appears alcohol consumption during the evening following competitive rugby matches may have some detrimental effects on peak power and cognitive recovery the morning following a Rugby League match. Accordingly, practitioners should be aware of the potential associated detrimental effects of alcohol consumption on recovery and provide alcohol awareness to athletes at post-match functions.

Alcohol ingestion impairs maximal post-exercise rates of myofibrillar protein synthesis following a single bout of concurrent training

Introduction The culture in many team sports involves consumption of large amounts of alcohol after training/competition. The effect of such a practice on recovery processes underlying protein turnover in human skeletal muscle are unknown. We determined the effect of alcohol intake on rates of myofibrillar protein synthesis (MPS) following strenuous exercise with carbohydrate (CHO) or protein ingestion. Methods In a randomized cross-over design, 8 physically active males completed three experimental trials comprising resistance exercise (8×5 reps leg extension, 80% 1 repetition maximum) followed by continuous (30 min, 63% peak power output (PPO)) and high intensity interval (10×30 s, 110% PPO) cycling. Immediately, and 4 h post-exercise, subjects consumed either 500 mL of whey protein (25 g; PRO), alcohol (1.5 g·kg body mass−1, 12±2 standard drinks) co-ingested with protein (ALC-PRO), or an energy-matched quantity of carbohydrate also with alcohol (25 g maltodextrin; ALC-CHO). Subjects also consumed a CHO meal (1.5 g CHO·kg body mass−1) 2 h post-exercise. Muscle biopsies were taken at rest, 2 and 8 h post-exercise. Results Blood alcohol concentration was elevated above baseline with ALC-CHO and ALC-PRO throughout recovery (P<0.05). Phosphorylation of mTORSer2448 2 h after exercise was higher with PRO compared to ALC-PRO and ALC-CHO (P<0.05), while p70S6K phosphorylation was higher 2 h post-exercise with ALC-PRO and PRO compared to ALC-CHO (P<0.05). Rates of MPS increased above rest for all conditions (~29–109%, P<0.05). However, compared to PRO, there was a hierarchical reduction in MPS with ALC-PRO (24%, P<0.05) and with ALC-CHO (37%, P<0.05). Conclusion We provide novel data demonstrating that alcohol consumption reduces rates of MPS following a bout of concurrent exercise, even when co-ingested with protein. We conclude that alcohol ingestion suppresses the anabolic response in skeletal muscle and may therefore impair recovery and adaptation to training and/or subsequent performance.