AGC : Aplicació per la gestió de continguts d'un portal

Els objectius principals del TFC són el d'anàlisis, disseny, implementació i test d'una aplicació per la gestió de continguts (AGC)

AGC-AE

Each issue has a distinctive title.

Cuesta Arriba. Antecedentes epistemológicos y metodológicos para una Evaluación Ambiental Estratégica -EAE- en la ejecución de Políticas Planes y Programas ambientales en el Area del Gran Catamarca -AGC-

En esta comunicación se informa sobre estudios socio ambientales realizados desde las perspectivas epistemológica y metodológica, en el Area del Gran Catamarca. El propósito es contribuir a una Evaluación Estratégica Ambiental. Se examinan antecedentes y se identifica la percepción de la problemática ambiental en diferentes sectores de población.. Para la construcción del Marco teórico se analizaron información escrita (libros, revistas periódicos) y webgrafia e infografia, de origen local, nacional e internacional. El abordaje transversal de la investigación revela la complejidad epistemológica del objeto de estudio cuando se busca alcanzar una visión contemporánea, global y contextualizadora del problema. Complejidad que se evidencia en los resultados de los análisis, de contenidos, multicriterial y hermeneutico. Las indagaciones sobre percepción de la problemática ambiental recogieron testimonios orales, cuestionarios y encuestas cuyas características se describen en el trabajo. Integrantes y alumnos de la cátedra Introducción a la Investigación (Filosofía y Ciencias de la Educación), realizaron actividades de educación ambiental y prácticas solidarias desde 1999 a 2002, como forma de extensión, transferencia e inserción social de la Universidad. La metodología de investigación-acción participativa optimizó resultados. Otra fuente de datos fueron entrevistas radiales (2001-2003), durante la emisión del Programa del LIPA- Hombre y Ambiente, emitido por Radio Universidad. En la Conclusión estimamos que los antecedentes de EAE revelan que conviene aplicarla en nuestro medio y que la percepción de los problemas de mayor impacto o riesgo ambiental en pobladores del AGC evidencia conocimiento, pero falta mayor compromiso para asumir la propia responsabilidad. Enunciamos fortalezas, debilidades y recomendaciones es por tales antecedentes que las tareas de transferencia y aplicaciones hace "Cuesta Arriba" por lo dificultosa, ya que deben remontarse una serie de inconvenientes algunos de los cuales se han enunciado en la presente comunicación

Cuesta Arriba. Antecedentes epistemológicos y metodológicos para una Evaluación Ambiental Estratégica -EAE- en la ejecución de Políticas Planes y Programas ambientales en el Area del Gran Catamarca -AGC-

En esta comunicación se informa sobre estudios socio ambientales realizados desde las perspectivas epistemológica y metodológica, en el Area del Gran Catamarca. El propósito es contribuir a una Evaluación Estratégica Ambiental. Se examinan antecedentes y se identifica la percepción de la problemática ambiental en diferentes sectores de población.. Para la construcción del Marco teórico se analizaron información escrita (libros, revistas periódicos) y webgrafia e infografia, de origen local, nacional e internacional. El abordaje transversal de la investigación revela la complejidad epistemológica del objeto de estudio cuando se busca alcanzar una visión contemporánea, global y contextualizadora del problema. Complejidad que se evidencia en los resultados de los análisis, de contenidos, multicriterial y hermeneutico. Las indagaciones sobre percepción de la problemática ambiental recogieron testimonios orales, cuestionarios y encuestas cuyas características se describen en el trabajo. Integrantes y alumnos de la cátedra Introducción a la Investigación (Filosofía y Ciencias de la Educación), realizaron actividades de educación ambiental y prácticas solidarias desde 1999 a 2002, como forma de extensión, transferencia e inserción social de la Universidad. La metodología de investigación-acción participativa optimizó resultados. Otra fuente de datos fueron entrevistas radiales (2001-2003), durante la emisión del Programa del LIPA- Hombre y Ambiente, emitido por Radio Universidad. En la Conclusión estimamos que los antecedentes de EAE revelan que conviene aplicarla en nuestro medio y que la percepción de los problemas de mayor impacto o riesgo ambiental en pobladores del AGC evidencia conocimiento, pero falta mayor compromiso para asumir la propia responsabilidad. Enunciamos fortalezas, debilidades y recomendaciones es por tales antecedentes que las tareas de transferencia y aplicaciones hace "Cuesta Arriba" por lo dificultosa, ya que deben remontarse una serie de inconvenientes algunos de los cuales se han enunciado en la presente comunicación

Cuesta Arriba. Antecedentes epistemológicos y metodológicos para una Evaluación Ambiental Estratégica -EAE- en la ejecución de Políticas Planes y Programas ambientales en el Area del Gran Catamarca -AGC-

En esta comunicación se informa sobre estudios socio ambientales realizados desde las perspectivas epistemológica y metodológica, en el Area del Gran Catamarca. El propósito es contribuir a una Evaluación Estratégica Ambiental. Se examinan antecedentes y se identifica la percepción de la problemática ambiental en diferentes sectores de población.. Para la construcción del Marco teórico se analizaron información escrita (libros, revistas periódicos) y webgrafia e infografia, de origen local, nacional e internacional. El abordaje transversal de la investigación revela la complejidad epistemológica del objeto de estudio cuando se busca alcanzar una visión contemporánea, global y contextualizadora del problema. Complejidad que se evidencia en los resultados de los análisis, de contenidos, multicriterial y hermeneutico. Las indagaciones sobre percepción de la problemática ambiental recogieron testimonios orales, cuestionarios y encuestas cuyas características se describen en el trabajo. Integrantes y alumnos de la cátedra Introducción a la Investigación (Filosofía y Ciencias de la Educación), realizaron actividades de educación ambiental y prácticas solidarias desde 1999 a 2002, como forma de extensión, transferencia e inserción social de la Universidad. La metodología de investigación-acción participativa optimizó resultados. Otra fuente de datos fueron entrevistas radiales (2001-2003), durante la emisión del Programa del LIPA- Hombre y Ambiente, emitido por Radio Universidad. En la Conclusión estimamos que los antecedentes de EAE revelan que conviene aplicarla en nuestro medio y que la percepción de los problemas de mayor impacto o riesgo ambiental en pobladores del AGC evidencia conocimiento, pero falta mayor compromiso para asumir la propia responsabilidad. Enunciamos fortalezas, debilidades y recomendaciones es por tales antecedentes que las tareas de transferencia y aplicaciones hace "Cuesta Arriba" por lo dificultosa, ya que deben remontarse una serie de inconvenientes algunos de los cuales se han enunciado en la presente comunicación

Phylogenetic placement of the enigmatic parasite, Polypodium hydriforme, within the Phylum Cnidaria

Background: Polypodium hydriforme is a parasite with an unusual life cycle and peculiar morphology, both of which have made its systematic position uncertain. Polypodium has traditionally been considered a cnidarian because it possesses nematocysts, the stinging structures characteristic of this phylum. However, recent molecular phylogenetic studies using 18S rDNA sequence data have challenged this interpretation, and have shown that Polypodium is a close relative to myxozoans and together they share a closer affinity to bilaterians than cnidarians. Due to the variable rates of 18S rDNA sequences, these results have been suggested to be an artifact of long-branch attraction ( LBA). A recent study, using multiple protein coding markers, shows that the myxozoan Buddenbrockia, is nested within cnidarians. Polypodium was not included in this study. To further investigate the phylogenetic placement of Polypodium, we have performed phylogenetic analyses of metazoans with 18S and partial 28S rDNA sequences in a large dataset that includes Polypodium and a comprehensive sampling of cnidarian taxa. Results: Analyses of a combined dataset of 18S and partial 28S sequences, and partial 28S alone, support the placement of Polypodium within Cnidaria. Removal of the long-branched myxozoans from the 18S dataset also results in Polypodium being nested within Cnidaria. These results suggest that previous reports showing that Polypodium and Myxozoa form a sister group to Bilateria were an artifact of long-branch attraction. Conclusion: By including 28S rDNA sequences and a comprehensive sampling of cnidarian taxa, we demonstrate that previously conflicting hypotheses concerning the phylogenetic placement of Polypodium can be reconciled. Specifically, the data presented provide evidence that Polypodium is indeed a cnidarian and is either the sister taxon to Hydrozoa, or part of the hydrozoan clade, Leptothecata. The former hypothesis is consistent with the traditional view that Polypodium should be placed in its own cnidarian class, Polypodiozoa.

Vascular Endothelial Growth Factor Genetic Polymorphisms and Haplotypes in Women with Migraine

Vascular endothelial growth factor (VEGF) production is regulated by growth factors and inflammatory cytokines, and VEGF plays a role in migraine. We examined for the first time whether three functional polymorphisms in the promoter region of VEGF gene (C(-2578)A, G(-1154A), and G(-634C)) and VEGF haplotypes are associated with migraine. We studied 114 healthy women without migraine and 175 women with migraine (129 without aura, and 46 with aura). We found no differences in the distributions of VEGF genotypes and alleles (p > 0.05). However, the CAC haplotype was more frequent in controls than in migraine patients, and the AGC haplotype was more frequent in patients with migraine with aura than in controls (both p < 0.05). These findings suggest that VEGF haplotypes affect susceptibility to migraine.

Application of inter simple sequence repeat (ISSR) markers to plant genetics

Microsatellites or simple sequence repeats (SSRs) are ubiquitous in eukaryotic genomes. Single-locus SSR markers have been developed for a number of species, although there is a major bottleneck in developing SSR markers whereby flanking sequences must be known to design 5'-anchors for polymerase chain reaction (PCR) primers. Inter SSR (ISSR) fingerprinting was developed such that no sequence knowledge was required. Primers based on a repeat sequence, such as (CA)(n), can be made with a degenerate 3'-anchor, such as (CA)(8)RG or (AGC)(6)TY. The resultant PCR reaction amplifies the sequence between two SSRs, yielding a multilocus marker system useful for fingerprinting, diversity analysis and genome mapping. PCR products are radiolabelled with P-32 or P-33 via end-labelling or PCR incorporation, and separated on a polyacrylamide sequencing gel prior to autoradiographic visualisation. A typical reaction yields 20-100 bands per lane depending on the species and primer. We have used ISSR fingerprinting in a number of plant species, and report here some results on two important tropical species, sorghum and banana. Previous investigators have demonstrated that ISSR analysis usually detects a higher level of polymorphism than that detected with restriction fragment length polymorphism (RFLP) or random amplified polymorphic DNA (RAPD) analyses. Our data indicate that this is not a result of greater polymorphism genetically, but rather technical reasons related to the detection methodology used for ISSR analysis.

Controlo clássico vs. preditivo na regulação de frequência em sistemas de potência

Dissertação para obtenção do grau de Mestre em Engenharia Electrotécnica Ramo Automação e Electrónica Industrial

On performance of distributed model predictive control in power system frequency regulation

This paper describes the implementation of a distributed model predictive approach for automatic generation control. Performance results are discussed by comparing classical techniques (based on integral control) with model predictive control solutions (centralized and distributed) for different operational scenarios with two interconnected networks. These scenarios include variable load levels (ranging from a small to a large unbalance generated power to power consumption ratio) and simultaneously variable distance between the interconnected networks systems. For the two networks the paper also examines the impact of load variation in an island context (a network isolated from each other).

Optimization of the Sybr Green real time PCR for the detection of Human Herpes Virus type 6 (HHV-6)

HHV-6 is the etiological agent of Exanthem subitum which is considered the sixth most frequent disease in infancy. In immuno-compromised hosts, reactivation of latent HHV-6 infection may cause severe acute disease. We developed a Sybr Green Real Time PCR for HHV-6 and compared the results with nested conventional PCR. A 214 pb PCR derived fragment was cloned using pGEM-T easy from Promega system. Subsequently, serial dilutions were made in a pool of negative leucocytes from 10-6 ng/µL (equivalent to 2465.8 molecules/µL) to 10-9 (equivalent to 2.46 molecules/µL). Dilutions of the plasmid were amplified by Sybr Green Real Time PCR, using primers HHV3 (5' TTG TGC GGG TCC GTT CCC ATC ATA 3)'and HHV4 (5' TCG GGA TAG AAA AAC CTA ATC CCT 3') and by conventional nested PCR using primers HHV1 (outer): 5'CAA TGC TTT TCT AGC CGC CTC TTC 3'; HHV2 (outer): 5' ACA TCT ATA ATT TTA GAC GAT CCC 3'; HHV3 (inner) and HHV4 (inner) 3'. The detection threshold was determined by plasmid serial dilutions. Threshold for Sybr Green real time PCR was 24.6 molecules/µL and for the nested PCR was 2.46 molecules/µL. We chose the Real Time PCR for diagnosing and quantifying HHV-6 DNA from samples using the new Sybr Green chemistry due to its sensitivity and lower risk of contamination.

Myeloid Sirtuin 2 expression does not impact long-term Mycobacterium tuberculosis control

Sirtuins (Sirts) regulate several cellular mechanisms through deacetylation of several transcription factors and enzymes. Recently, Sirt2 was shown to prevent the development of inflammatory processes and its expression favors acute Listeria monocytogenes infection. The impact of this molecule in the context of chronic infections remains unknown. We found that specific Sirt2 deletion in the myeloid lineage transiently increased Mycobacterium tuberculosis load in the lungs and liver of conditional mice. Sirt2 did not affect long-term infection since no significant differences were observed in the bacterial burden at days 60 and 120 post-infection. The initial increase in M. tuberculosis growth was not due to differences in inflammatory cell infiltrates in the lung, myeloid or CD4+ T cells. The transcription levels of IFN-?, IL-17, TNF, IL-6 and NOS2 were also not affected in the lungs by Sirt2-myeloid specific deletion. Overall, our results demonstrate that Sirt2 expression has a transitory effect in M. tuberculosis infection. Thus, modulation of Sirt2 activity in vivo is not expected to affect chronic infection with M. tuberculosis.

IL-17A promotes intracellular growth of Mycobacterium by inhibiting apoptosis of infected macrophages

The fate of infected macrophages is a critical aspect of immunity to mycobacteria. By depriving the pathogen of its intracellular niche, apoptotic death of the infected macrophage has been shown to be an important mechanism to control bacterial growth. Here, we show that IL-17 inhibits apoptosis of Mycobacterium bovis BCG- or Mycobacterium tuberculosis-infected macrophages thus hampering their ability to control bacterial growth. Mechanistically, we show that IL-17 inhibits p53, and impacts on the intrinsic apoptotic pathway, by increasing the Bcl2 and decreasing Bax expression, decreasing cytochrome c release from the mitochondria, and inhibiting caspase-3 activation. The same effect of IL-17 was observed in infected macrophages upon blockade of p53 nuclear translocation. These results reveal a previously unappreciated role for the IL-17/p53 axis in the regulation of mycobacteria-induced apoptosis and can have important implications in a broad spectrum of diseases where apoptosis of the infected cell is an important host defense mechanism.

Detection of rifampin resistance patterns in Mycobacterium tuberculosis strains isolated in Iran by polymerase chain reaction-single-strand conformation polymorphism and direct sequencing methods

Mutations in the rpoB locus confer conformational changes leading to defective binding of rifampin (RIF) to rpoB and consequently resistance in Mycobacterium tuberculosis. Polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) was established as a rapid screening test for the detection of mutations in the rpoB gene, and direct sequencing has been unambiguously applied to characterize mutations. A total of 37 of Iranian isolates of M. tuberculosis, 16 sensitive and 21 resistant to RIF, were used in this study. A 193-bp region of the rpoB gene was amplified and PCR-SSCP patterns were determined by electrophoresis in 10% acrylamide gel and silver staining. Also, 21 samples of 193-bp rpoB amplicons with different PCR-SSCP patterns from RIFr and 10 from RIFs were sequenced. Seven distinguishable PCR-SSCP patterns were recognized in the 21 Iranian RIFr strains, while 15 out of 16 RIFs isolates demonstrated PCR-SSCP banding patterns similar to that of sensitive standard strain H37Rv. However one of the sensitive isolates demonstrated a different pattern. There were seen six different mutations in the amplified region of rpoB gene: codon 516(GAC/GTC), 523(GGG/GGT), 526(CAC/TAC), 531(TCG/TTG), 511(CTG/TTG), and 512(AGC/TCG). This study demonstrated the high specificity (93.8%) and sensitivity (95.2%) of PCR-SSCP method for detection of mutation in rpoB gene; 85.7% of RIFr strains showed a single mutation and 14.3% had no mutations. Three strains showed mutations caused polymorphism. Our data support the common notion that rifampin resistance genotypes are generally present mutations in codons 531 and 526, most frequently found in M. tuberculosis populations regardless of geographic origin.

In house colorimetric reverse hybridisation assay for detection of the mutation most frequently associated with resistance to isoniazid in Mycobacterium tuberculosis

Mutations in the katG gene have been identified and correlated with isoniazid (INH) resistance in Mycobacterium tuberculosis isolates. The mutation AGC→ACC (Ser→Thr) at katG315 has been reported to be the most frequent and is associated with transmission and multidrug resistance. Rapid detection of this mutation could therefore improve the choice of an adequate anti-tuberculosis regimen, the epidemiological monitoring of INH resistance and, possibly, the tracking of transmission of resistant strains. An in house reverse hybridisation assay was designed in our laboratory and evaluated with 180 isolates of M. tuberculosis. It could successfully characterise the katG315 mutation in 100% of the samples as compared to DNA sequencing. The test is efficient and is a promising alternative for the rapid identification of INH resistance in regions with a high prevalence of katG315 mutants.