Classificação multi-etiqueta hierárquica de textos segundo a taxonomia ACM

Mestrado em Engenharia Informática

ACM/IEEE Curricula ja tietotekniikan osaston kandidaatin tutkinnon vertailu, arviointi ja soveltaminen

Tietotekniikan käyttö on yleistynyt sekä yritysmaailmassa että kotikäytössä viimeisen kahden vuosikymmenen aikana. Tietotekniikan opetuksen ajanmukaisuus onkin tärkeää, jotta yritykset saisivat palkattua tarpeitaan vastaavia tietotekniikan ammattilaisia. Tässä kandidaatintyössä perehdytään ACM/IEEE:n opetussuunnitelmiin vertailemalla ja arvioimalla niitä Lappeenrannan teknillisen yliopiston tietotekniikan kandidaatin tutkintoon. Työssä tehtävä vertailu ja arviointi pohjautuvat kirjallisuuteen sekä haastatteluihin Lappeenrannan teknillisen yliopiston tietotekniikan osastolla.

Incidencia del Acuerdo Comercial Multipartes, ACM en el Sistema Nacional de Contratación Pública

El presente trabajo trata sobre la apertura del mercado de la contratación pública prevista en el Acuerdo Comercial Multipartes (ACM) y las incidencias que generará en el Sistema Nacional de Contratación Pública (SNCP) y en las herramientas informáticas del Sistema Oficial de Contratación del Estado (SOCE). Habiendo transcurrido más de un año desde que en julio de 2014 la Comisión Negociadora del Ecuador suscribió el acuerdo preliminar, no se han generado acciones tendentes a informar y capacitar a los operadores del sistema (entidades contratantes, proveedores, organismos de control, organismo rector, veedurías) ni se han tomado medidas destinadas a la adecuación de las herramientas electrónicas con las que opera el portal institucional del SERCOP. El país desconoce el alcance del ACM en materia de contratación pública, qué sectores productivos podrían verse afectados con el libre comercio, qué modificaciones se realizarían a las condiciones de participación, si se mantendrá igual o con ciertas limitaciones el sistema de preferencias que la Constitución de la República y la Ley Orgánica del Sistema Nacional de Contratación Pública prevén para la producción nacional en especial aquella que proviene de micro, pequeñas y medianas empresas y de los actores de la economía popular y solidaria. Acercarnos a esta temática a través de la revisión y análisis jurídicos del mencionado acuerdo internacional, limitándolo a la materia de contratación pública, comparándolo con el ordenamiento jurídico ecuatoriano a fin de encontrar, por una parte, los retos y oportunidades; y, por otra, realizar con los datos que nos proporciona el SOCE el cálculo aproximado del porcentaje de apertura del mercado de contratación pública al libre comercio, a fin de encontrar mecanismos que contribuyan a sustentar la política de compras públicas destinada al desarrollo de la economía nacional; temas éstos que son el lietmotiv de esta aportación.

Contribution of the collagen adhesin Acm to pathogenesis of Enterococcus faecium in experimental endocarditis.

Enterococcus faecium is a multidrug-resistant opportunist causing difficult-to-treat nosocomial infections, including endocarditis, but there are no reports experimentally demonstrating E. faecium virulence determinants. Our previous studies showed that some clinical E. faecium isolates produce a cell wall-anchored collagen adhesin, Acm, and that an isogenic acm deletion mutant of the endocarditis-derived strain TX0082 lost collagen adherence. In this study, we show with a rat endocarditis model that TX0082 Deltaacm::cat is highly attenuated versus wild-type TX0082, both in established (72 h) vegetations (P < 0.0001) and for valve colonization 1 and 3 hours after infection (P or=50-fold reduction relative to an Acm producer) were found in three of these five nonadherent isolates, including the sequenced strain TX0016, by quantitative reverse transcription-PCR, indicating that acm transcription is downregulated in vitro in these isolates. However, examination of TX0016 cells obtained directly from infected rat vegetations by flow cytometry showed that Acm was present on 40% of cells grown during infection. Finally, we demonstrated a significant reduction in E. faecium collagen adherence by affinity-purified anti-Acm antibodies from E. faecium endocarditis patient sera, suggesting that Acm may be a potential immunotarget for strategies to control this emerging pathogen.

A functional collagen adhesin gene, acm, in clinical isolates of Enterococcus faecium correlates with the recent success of this emerging nosocomial pathogen.

Enterococcus faecium recently evolved from a generally avirulent commensal into a multidrug-resistant health care-associated pathogen causing difficult-to-treat infections, but little is known about the factors responsible for this change. We previously showed that some E. faecium strains express a cell wall-anchored collagen adhesin, Acm. Here we analyzed 90 E. faecium isolates (99% acm(+)) and found that the Acm protein was detected predominantly in clinically derived isolates, while the acm gene was present as a transposon-interrupted pseudogene in 12 of 47 isolates of nonclinical origin. A highly significant association between clinical (versus fecal or food) origin and collagen adherence (P

Construction of improved temperature-sensitive and mobilizable vectors and their use for constructing mutations in the adhesin-encoding acm gene of poorly transformable clinical Enterococcus faecium strains.

Inactivation by allelic exchange in clinical isolates of the emerging nosocomial pathogen Enterococcus faecium has been hindered by lack of efficient tools, and, in this study, transformation of clinical isolates was found to be particularly problematic. For this reason, a vector for allelic replacement (pTEX5500ts) was constructed that includes (i) the pWV01-based gram-positive repAts replication region, which is known to confer a high degree of temperature intolerance, (ii) Escherichia coli oriR from pUC18, (iii) two extended multiple-cloning sites located upstream and downstream of one of the marker genes for efficient cloning of flanking regions for double-crossover mutagenesis, (iv) transcriptional terminator sites to terminate undesired readthrough, and (v) a synthetic extended promoter region containing the cat gene for allelic exchange and a high-level gentamicin resistance gene, aph(2'')-Id, to distinguish double-crossover recombination, both of which are functional in gram-positive and gram-negative backgrounds. To demonstrate the functionality of this vector, the vector was used to construct an acm (encoding an adhesin to collagen from E. faecium) deletion mutant of a poorly transformable multidrug-resistant E. faecium endocarditis isolate, TX0082. The acm-deleted strain, TX6051 (TX0082Deltaacm), was shown to lack Acm on its surface, which resulted in the abolishment of the collagen adherence phenotype observed in TX0082. A mobilizable derivative (pTEX5501ts) that contains oriT of Tn916 to facilitate conjugative transfer from the transformable E. faecalis strain JH2Sm::Tn916 to E. faecium was also constructed. Using this vector, the acm gene of a nonelectroporable E. faecium wound isolate was successfully interrupted. Thus, pTEX5500ts and its mobilizable derivative demonstrated their roles as important tools by helping to create the first reported allelic replacement in E. faecium; the constructed this acm deletion mutant will be useful for assessing the role of acm in E. faecium pathogenesis using animal models.

Inhibition of Enterococcus faecium adherence to collagen by antibodies against high-affinity binding subdomains of Acm.

Strains of Enterococcus faecium express a cell wall-anchored protein, Acm, which mediates adherence to collagen. Here, we (i) identify the minimal and high-affinity binding subsegments of Acm and (ii) show that anti-Acm immunoglobulin Gs (IgGs) purified against these subsegments reduced E. faecium TX2535 strain collagen adherence up to 73 and 50%, respectively, significantly more than the total IgGs against the full-length Acm A domain (28%) (P < 0.0001). Blocking Acm adherence with functional subsegment-specific antibodies raises the possibility of their use as therapeutic or prophylactic agents.