Cellular settings mediating Src Substrate switching between Focal Adhesion Kinase Tyrosine 861 and CUB-domain-containing protein 1 (CDCP1) Tyrosine 734

Reciprocal interactions between Src family kinases (SFKs) and focal adhesion kinase (FAK) are critical during changes in cell attachment. Recently it has been recognized that another SFK substrate, CUB-domain-containing protein 1 (CDCP1), is differentially phosphorylated during these events. However, the molecular processes underlying SFK-mediated phosphorylation of CDCP1 are poorly understood. Here we identify a novel mechanism in which FAK tyrosine 861 and CDCP1-Tyr-734 compete as SFK substrates and demonstrate cellular settings in which SFKs switch between these sites. Our results show that stable CDCP1 expression induces robust SFK-mediated phosphorylation of CDCP1-Tyr-734 with concomitant loss of p-FAK-Tyr-861 in adherent HeLa cells. SFK substrate switching in these cells is dependent on the level of expression of CDCP1 and is also dependent on CDCP1-Tyr-734 but is independent of CDCP1-Tyr-743 and -Tyr-762. In HeLa CDCP1 cells, engagement of SFKs with CDCP1 is accompanied by an increase in phosphorylation of Src-Tyr-416 and a change in cell morphology to a fibroblastic appearance dependent on CDCP1-Tyr-734. SFK switching between FAK-Tyr-861 and CDCP1-Tyr-734 also occurs during changes in adhesion of colorectal cancer cell lines endogenously expressing these two proteins. Consistently, increased p-FAK-Tyr-861 levels and a more epithelial morphology are seen in colon cancer SW480 cells silenced for CDCP1. Unlike protein kinase Cδ, FAK does not appear to form a trimeric complex with Src and CDCP1. These data demonstrate novel aspects of the dynamics of SFK-mediated cell signaling that may be relevant during cancer progression.

Diário da Constituinte [gravação de vídeo] : [programa n. 734]

Informa novo prazo para que constituintes apresentem suas propostas, para a nova carta. Texto sobre tabelamento dos juros em 12% provoca polêmica entre os constituintes. O PFL não aceitou e pretende tomar providências com relação à medida. A população que foi entrevistada, demonstrou grande satisfação com a medida e acredita que ela é justa para o país. Texto corrigido pela Comissão de Redação Final está sendo distribuído aos constituintes. Alguns constituintes sugeriram votação em separado, mas o Presidente Ulysses Guimarães garante que o regimento será cumprido, e que a votação do texto será em in loco, em um único dia.

Manual de la ley 734 de 2002 dirigido a los servidores públicos del país no abogados

El lector tiene entre sus manos un manual esencialmente práctico, el interés ha sido sobre todo recopilar y ordenar los datos normativos y jurisprudenciales existentes en nuestro ordenamiento sobre régimen disciplinario de los funcionarios públicos

Procedimientos especiales-Régimen disciplinario Ley 734 de 2002

El Código Disciplinario Único requiere un tratamiento que le permita a los servidores públicos adelantar y fallar con seguridad los procesos disciplinarios, respetando los derechos y garantías que el ordenamiento jurídico otorga a los investigados

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 734 - Notarielles Verfahren einer ausgestellten Quittung an Gottlieb Wilhelm Skerle

u.a.: unterschiedliche Rechtssysteme in Deutschland; Auseinandersetzung mit Karl Labes;