Effect of Laser (670 nm) on Healing of Wounds Covered with Occlusive Dressing: A Histologic and Biomechanical Analysis

Objectives: To analyze the effects of low-level laser therapy (LLLT), 670 nm, with doses of 4 and 7 J/cm(2), on the repair of surgical wounds covered by occlusive dressings. Background Data: The effect of LLLT on the healing process of covered wounds is not well defined. Materials and Methods: For the histologic analysis with HE staining, 50 male Wistar rats were submitted to surgical incisions and divided into 10 groups (n=5): control; stimulated with 4 and 7 J/cm(2) daily, for 7 and 14 days, with or without occlusion. Reepithelization and the number of leukocytes, fibroblasts, and fibrocytes were obtained with an image processor. For the biomechanical analysis, 25 rats were submitted to a surgical incision and divided into five groups (n=5): treated for 14 days with and without occlusive dressing, and the sham group. Samples of the lesions were collected and submitted to the tensile test. One-way analysis of variance was performed, followed by post hoc analysis. A Tukey test was used on the biomechanical data, and the Tamhane test on the histologic data. A significance level of 5% was chosen (p <= 0.05). Results: The 4 and 7J/cm(2) laser with and without occlusive dressing did not alter significantly the reepithelization rate of the wounds. The 7 J/cm(2) laser reduced the number of leukocytes significantly. The number of fibroblasts was higher in the groups treated with laser for 7 days, and was significant in the covered 4 J/cm(2) laser group. Conclusions: Greater interference of the laser-treatment procedure was noted with 7 days of stimulation, and the occlusive dressing did not alter its biostimulatory effects.

Expression of MYC, IgM, As Well As Non-Germinal Centre B-Cell Like Immunophenotype and Positive Immunofish Index Predict a Worse Progression Free Survival and Overall Survival in a Series of 670 De Novo Diffuse Large B-Cell Lymphomas Included in Clinical Trials: A GELA Study of the 2003 Program

Introduction: Diffuse large B-cell lymphomas (DLBCL) represent a heterogeneous disease with variable clinical outcome. Identifying phenotypic biomarkers of tumor cells on paraffin sections that predict different clinical outcome remain an important goal that may also help to better understand the biology of this lymphoma. Differentiating non-germinal centre B-cell-like (non-GCB) from Germinal Centre B-cell-like (GCB) DLBCL according to Hans algorithm has been considered as an important immunohistochemical biomarker with prognostic value among patients treated with R-CHOP although not reproducibly found by all groups. Gene expression studies have also shown that IgM expression might be used as a surrogate for the GCB and ABC subtypes with a strong preferential expression of IgM in ABC DLBCL subtype. ImmunoFISH index based on the differential expression of MUM-1, FOXP1 by immunohistochemistry and on the BCL6 rearrangement by FISH has been previously reported (C Copie-Bergman, J Clin Oncol. 2009;27:5573-9) as prognostic in an homogeneous series of DLBCL treated with R-CHOP. In addition, oncogenic MYC protein overexpression by immunohistochemistry may represent an easy tool to identify the consequences of MYC deregulation in DLBCL. Our aim was to analyse by immunohistochemistry the prognostic relevance of MYC, IgM, GCB/nonGCB subtype and ImmunoFISH index in a large series of de novo DLBCL treated with Rituximab (R)-chemotherapy (anthracyclin based) included in the 2003 program of the Groupe d'Etude des Lymphomes de l'Adulte (GELA) trials. Methods: The 2003 program included patients with de novo CD20+ DLBCL enrolled in 6 different LNH-03 GELA trials (LNH-03-1B, -B, -3B, 39B, -6B, 7B) stratifying patients according to age and age-adjusted IPI. Tumor samples were analyzed by immunohistochemistry using CD10, BCL6, MUM1, FOXP1 (according to Barrans threshold), MYC, IgM antibodies on tissue microarrays and by FISH using BCL6 split signal DNA probes. Considering evaluable Hans score, 670 patients were included in the study with 237 (35.4%) receiving intensive R-ACVBP regimen and 433 (64.6%) R-CHOP/R-mini-CHOP. Results: 304 (45.4%) DLBCL were classified as GCB and 366 (54.6%) as non-GCB according to Hans algorithm. 337/567 cases (59.4%) were positive for the ImmunoFISH index (i.e. two out of the three markers positive: MUM1 protein positive, FOXP1 protein Variable or Strong, BCL6 rearrangement). Immunofish index was preferentially positive in the non-GCB subtype (81.3%) compared to the GCB subtype (31.2%), (p<0.001). IgM was recorded as positive in tumor cells in 351/637 (52.4%) DLBCL cases with a preferential expression in non-GCB 195 (53.3%) vs GCB subtype 100(32.9%), p<0.001). MYC was positive in 170/577 (29.5%) cases with a 40% cut-off and in 44/577 (14.2%) cases with a cut-off of 70%. There was no preferential expression of MYC among GCB or non-GCB subtype (p>0.4) for both cut-offs. Progression-free Survival (PFS) was significantly worse among patients with high IPI score (p<0.0001), IgM positive tumor (p<0.0001), MYC positive tumor with a 40% threshold (p<0.001), ImmunoFISH positive index (p<0.002), non-GCB DLBCL subtype (p<0.0001). Overall Survival (OS) was also significantly worse among patients with high IPI score (p<0.0001), IgM positive tumor (p=0.02), MYC positive tumor with a 40% threshold (p<0.01), ImmunoFISH positive index (p=0.02), non-GCB DLBCL subtype (p<0.0001). All significant parameters were included in a multivariate analysis using Cox Model and in addition to IPI, only the GCB/non-GCB subtype according to Hans algorithm predicted significantly a worse PFS among non-GCB subgroup (HR 1.9 [1.3-2.8] p=0.002) as well as a worse OS (HR 2.0 [1.3-3.2], p=0.003). This strong prognostic value of non-GCB subtyping was confirmed considering only patients treated with R- CHOP for PFS (HR 2.1 [1.4-3.3], p=0.001) and for OS (HR 2.3 [1.3-3.8], p=0.002). Conclusion: Our study on a large series of patients included in trials confirmed the relevance of immunohistochemistry as a useful tool to identify significant prognostic biomarkers for clinical use. We show here that IgM and MYC might be useful prognostic biomarkers. In addition, we confirmed in this series the prognostic value of the ImmunoFISH index. Above all, we fully validated the strong and independent prognostic value of the Hans algorithm, daily used by the pathologists to subtype DLBCL.

Iowa DOT Library Services, Collection, & Technology Assessment, TR-670, 2014

Assesses the impact of library services on research projects, proposes methods to improve the impact of library services on research projects, assesses current library technology systems and proposes upgrades, assesses current library collection infrastructure and propose upgrades, especially in regards to collection damage from water or fire; ascertains patron interest in mobile technologies and suggest development based on interest.

1.° Genealogia, ortus, vel actus Caroli, gloriosi atque piissimi Imperatoris ; sive potiùs chronicum Anianense, ab anno 670. ad an. 821. decimo tertio saeculo exaratum. — 2.° Gesta Comitum Barcinonensium et Regum Aragoniae : decimo quarto saeculo exarata. — 3.° Epicedium in funere Raimundi, Comitis Tolosani : duodecimo saeculo exaratum. — 4.° Presbyteri Joannis epistola ad Emmanuëlem, Romaeorum Imperatorem : decimo tertio saeculo exarata. — 5.° Concilium Romae habitum anno nono pontificatus Gregorii Papae VII. adversùs Guibertum, Ravennatem Archiepiscopum : decimo tertio saeculo exaratum.

Baluzianus

Low-level laser therapy (670 nm) on viability of random skin flap in rats

This study investigated the effects of 670 nm laser, at different fluences, on the viability of skin flap in rats. One hundred male animals were used. The animals were divided into control group; group treated with 3 J/cm(2); group treated with 6 J/cm(2); group treated with 12 J/cm(2) and group treated with 24 J/cm(2). The skin flap was made on the backs of all animals studied, with a plastic sheet interposed between the flap and the donor site. Laser irradiation was done immediately after the surgery and on days 1, 2, 3 and 4 after surgery. The percentage of necrosis of the flap was calculated at the 7th postoperative day. Additionally, a sample of each flap was collected to enable us to count the blood vessels. Treated animals showed a statistically significant smaller area of necrosis than did the control group. The necrosis in the treated groups was 41.82% (group 2), 36.51% (group 3), 29.45% (group 4) and 20.37% (group 5). We also demonstrated that laser irradiation at 670 nm, at all doses used, had a stimulatory effect on angiogenesis. Our study showed that the 670 nm laser was efficient to increase the viability of the skin flap, at all fluences used, with a tendency of reaching better results at higher doses.

Resolución 670(XXXIV): Comité Especial de la Comisión Económica para América Latina y el Caribe sobre Población y Desarrollo

Incluye Bibliografía

Resolución 670(XXXIV): Comité Especial de la Comisión Económica para América Latina y el Caribe sobre Población y Desarrollo

Versión en inglés disponible en Biblioteca

Resolution 670(XXXIV) Ad Hoc Committee on Population and Development of the Economic Commission for Latin America and the Caribbean

Spanish version available

Report of the ECLAC Ad Hoc Committee on Population and Development and resolution 670(XXXIV) Ad Hoc Committee on Population and Development of the Economic Commision for Latin America and the Caribbean (Rev.1)

Includes bibliography

Informe del Comité Especial de la CEPAL sobre Población y Desarrollo, resolución 670(XXXIV) del Trigésimo Cuarto Período de Sesiones de la CEPAL (Rev.1)

Incluye Bibliografía.

Effects of 830 and 670 nm Laser on Viability of Random Skin Flap in Rats

Objective: This study aimed to investigate the effect of 830 and 670 nm diode laser on the viability of random skin flaps in rats. Background data: Low-level laser therapy (LLLT) has been reported to be successful in stimulating the formation of new blood vessels and reducing the inflammatory process after injury. However, the efficiency of such treatment remains uncertain, and there is also some controversy regarding the efficacy of different wavelengths currently on the market. Materials and methods: Thirty Wistar rats were used and divided into three groups, with 10 rats in each. A random skin flap was raised on the dorsum of each animal. Group 1 was the control group, group 2 received 830 nm laser radiations, and group 3 was submitted to 670 nm laser radiation (power density = 0.5 mW/cm(2)). The animals underwent laser therapy with 36 J/cm(2) energy density (total energy = 2.52 J and 72 sec per session) immediately after surgery and on the 4 subsequent days. The application site of laser radiation was one point at 2.5 cm from the flap's cranial base. The percentage of skin flap necrosis area was calculated on the 7th postoperative day using the paper template method. A skin sample was collected immediately after to determine the vascular endothelial growth factor (VEGF) expression and the epidermal cell proliferation index (KiD67). Results: Statistically significant differences were found among the percentages of necrosis, with higher values observed in group 1 compared with groups 2 and 3. No statistically significant differences were found among these groups using the paper template method. Group 3 presented the highest mean number of blood vessels expressing VEGF and of cells in the proliferative phase when compared with groups 1 and 2. Conclusions: LLLT was effective in increasing random skin flap viability in rats. The 670 nm laser presented more satisfactory results than the 830 nm laser.

Na 50 Nachlass Arthur Schopenhauer - 'Schopenhauer-Archiv', 670 - Benachrichtigung über eine schwere Krankheit

u.a.: Verstopfung; Wassersucht; Kur in Karlsbad; Krankheit von Adele Schopenhauer; Erbschaftsangelegenheiten; Bankhaus Muhl;

Na 1 Nachlass Max Horkheimer, 670 - "Treatise on Antisemitism" und zugehörige Dokumente (p. IX 124-IX 134)

"Treatise on Antisemitism", Unterlagen zur Buchveröffentlichung, 1945-1946; "Tentative Outline of Contens", a) Typoskript, 6 Blatt; b) Typoskript, 2 Blatt; c) Typoskript, 2 Blatt; "Section 2: Modern European Antisemitism". Typoskript, 5 Blatt; "Section 4: Political Antisemitism in America". Typoskript, 5 Blatt; "Section 5: Religious and Social Ideologies". Typoskript, 7 Blatt; "The Nature of Anti-Semitism. Psychologocal Topics to be Surveyed", a) Typoskript, 2 Blatt; b) Typoskript, 2 Blatt; "Proposed Project for a Treatise on Antisemitism" und "Tentative Outline of Contents". Typoskript mit handschriftlichen Ergänzungen, 8 Blatt; "Notes on the Content for Sections on American Antisemitism for the proposed book". Typoskript, 9 Blatt; Über den Aufbau des Buchs, eigenhändige Notiz von Max Horkheimer, 1 Blatt; Löwenthal, Leo: "Memorandum to Max Horkheimer, re: books", 25.11.1946. Typoskript, 6 Blatt; Memoranden über Besprechungen betreffend "Traetise", 28.05-10.10.1945, Typoskript, 6 Blatt; Jaeger, Werner: 1 Brief mit Unterschrift an Gordon W. Allport, Cambridge, Mass., 21.06.1945, 1 Blatt; Statements for AJC on account 'Treatise', Juli 1945 - Mai 1947, 19 Blatt; Unterlagen zur deutschen Ausgabe der "Studies in Prejudice", 1950-1952; "German Version of the Series 'Studies in Prejudice'". Typoskript, 3 Blatt; "Bericht über den Plan zur Herstellung einer deutschen Fassung der Studies in Prejudice". Typoskript, 4 Blatt; Memoranden zur Arbeit an der deutschen Ausgabe, 15.01.1951- 05.12.1952, Typoskript, 7 Blatt; Record of Meeting Commentary and Institute of Social Research", 29.05.1946; Diskussionsteilnehmer: Cohen, Elliot; Glazer, Nathan; Greenberg, Clement; Warshow, Robert; Löwenthal, Leo; Massing, Paul; Pollock, Friedrich; Weil, Felix; Gurland, A.R.L.; Jahoda, Marie; Löwe, Adolf; Typoskript, 19 Blatt; "Some Notes to the 'tentative draft discussed with R.'", Datierung unklar, um 1943? Typoskript mit handschriftlichen Korrekturen, 4 Blatt; "Re: Antisemitism in occupied Europe", Datierung unklar, etwa 1945-1948?; Typsokript, 3 Blatt; "Studies Undertaken in the Project on Political Antisemitism", Tabellarische Aufzählung, Datierung unklar, Typoskript, 1 Blatt; Institut of Social Research: "Instructions", Anweisungen für Interviewer, Fragebogen, Datierung unklar, Entwurf, Typoskript, 4 Blatt; Über Forschungsunternehmungen zu ethischen Vorurteilesstrukturen in den USA zwischen 1928 und 1939, Datierung unklar, Typoskript, 2 Blatt; Institut of Social Research: "Section I: Protestantism and Antisemitism. Section II: Report on the General Body of Protestant Antisemitic Feeling", Datierung unklar, Typoskript, 29 Blatt; Horkheimer, Max: "Re: Anti-Semitism- Spearhead of Nazism", Datierung unklar, Typoskript, 6 Blatt; Adorno, Theodor W.: "Re: Questionaire on Anti-Semitism", Datierung unklar. Typoskript, 5 Blatt; Adorno, Theodor W.: "Outline of a socio-psychological study", Datierung unklar. Typoskript mit eigenhändigen Korrekturen, 4 Blatt; "Sample: Responsiveness of Types of Anti-Semites to Anti-Semitic Propaganda", 2 handschriftliche Tabellen-Schemata, Datierung unklar, 2 Blatt;