Sepharose 4B一步法对金环蛇蛇毒磷脂酶A_(2)的分离纯化

利用经酸处理的Sepharose 4B为层析介质,以含0.2mol/L半乳糖,pH7.4台氏液作为洗脱液,从广西产金环蛇(Bungarus fasciatus)蛇毒中一步分离得到一种磷脂酶A_(2)。用SDS-聚丙烯酰胺凝胶电泳测定其分子量为14kDa。N端部分序列测定表明,所分离得到的磷脂酶A_(2)其N端16个氨基酸残基序列与已报道的金环蛇蛇毒磷脂酶A_(2)同功酶Ⅵ(Lu＆Lo,1978)一致。该酶糖含量较高,为13.4%;具有弱的磷脂酶A_(2)活性,无毒,也无溶血和出血毒活性。

A baseline survey of water quality, invertebrates, fisheries and socioeconomics on Lake Edward for proposed seismic surveys in block 4B : Final report

The National Fisheries Resources Research Institute (NaFIRRI) on behalf of OPEP Consult Ltd undertook a baseline survey of the transition zone (basically along the shoreline) and near shore habitats of the Uganda apart of Lake Edward and Kazinga channel during December 2007 to January 2008. A major objective of the baseline survey was to generate baseline information on the aquatic ecosystem features related to the fisheries and socio-economics of the fish catch including issues raised by residents in the fish landing sites. Therefore, the baseline survey captured information on water quality, the aquatic invertebrate fauna, aspects of fish biology and ecology, the fish catch including facilities at fish landings, value in the catch and related fisheries socio-economic issues perceived by residents in the settled areas along the shores.

WASP-4b: A 12th magnitude transiting hot Jupiter in the southern hemisphere

We report the discovery of WASP-4b, a large transiting gas-giant planet with an orbital period of 1.34 days. This is the first planet to be discovered by the SuperWASP-South observatory and CORALIE collaboration and the first planet orbiting a star brighter than 16th magnitude to be discovered in the southern hemisphere. A simultaneous fit to high-quality light curves and precision radial velocity measurements leads to a planetary mass of 1.22(-0.08)(+0.09) M-Jup and a planetary radius of 1.42(-0.04)(+0.07) R-Jup. The host star is USNO-B1.0 0479-0948995, a G7 V star of visual magnitude 12.5. As a result of the short orbital period, the predicted surface temperature of the planet is 1761 K, making it an ideal candidate for detections of the secondary eclipse at infrared wavelengths.

Novel functional interaction between the plasma membrane Ca2+ pump 4b and the proapoptotic tumor suppressor Ras-associated factor 1 (RASSF1)

Plasma membrane calmodulin-dependent calcium ATPases (PMCAs) are enzymatic systems implicated in the extrusion of calcium from the cell. We and others have previously identified molecular interactions between the cytoplasmic COOH-terminal end of PMCA and PDZ domain-containing proteins. These interactions suggested a new role for PMCA as a modulator of signal transduction pathways. The existence of other intracellular regions in the PMCA molecule prompted us to investigate the possible participation of other domains in interactions with different partner proteins. A two-hybrid screen of a human fetal heart cDNA library, using the region 652-840 of human PMCA4b (located in the catalytic, second intracellular loop) as bait, revealed a novel interaction between PMCA4b and the tumor suppressor RASSF1, a Ras effector protein involved in H-Ras-mediated apoptosis. Immunofluorescence co-localization, immunoprecipitation, and glutathione S-transferase pull-down experiments performed in mammalian cells provided further confirmation of the physical interaction between the two proteins. The interaction domain has been narrowed down to region 74-123 of RASSF1C (144-193 in RASSF1A) and 652-748 of human PMCA4b. The functionality of this interaction was demonstrated by the inhibition of the epidermal growth factor-dependent activation of the Erk pathway when PMCA4b and RASSF1 were co-expressed. This inhibition was abolished by blocking PMCA/RASSSF1 association with an excess of a green fluorescent protein fusion protein containing the region 50-123 of RASSF1C. This work describes a novel protein-protein interaction involving a domain of PMCA other than the COOH terminus. It suggests a function for PMCA4b as an organizer of macromolecular protein complexes, where PMCA4b could recruit diverse proteins through interaction with different domains. Furthermore, the functional association with RASSF1 indicates a role for PMCA4b in the modulation of Ras-mediated signaling.

Identification of a novel determinant for membrane association in hepatitis C virus nonstructural protein 4B.

Nonstructural protein 4B (NS4B) plays an essential role in the formation of the hepatitis C virus (HCV) replication complex. It is a relatively poorly characterized integral membrane protein predicted to comprise four transmembrane segments in its central portion. Here, we describe a novel determinant for membrane association represented by amino acids (aa) 40 to 69 in the N-terminal portion of NS4B. This segment was sufficient to target and tightly anchor the green fluorescent protein to cellular membranes, as assessed by fluorescence microscopy as well as membrane extraction and flotation analyses. Circular dichroism and nuclear magnetic resonance structural analyses showed that this segment comprises an amphipathic alpha-helix extending from aa 42 to 66. Attenuated total reflection infrared spectroscopy and glycosylation acceptor site tagging revealed that this amphipathic alpha-helix has the potential to traverse the phospholipid bilayer as a transmembrane segment, likely upon oligomerization. Alanine substitution of the fully conserved aromatic residues on the hydrophobic helix side abrogated membrane association of the segment comprising aa 40 to 69 and disrupted the formation of a functional replication complex. These results provide the first atomic resolution structure of an essential membrane-associated determinant of HCV NS4B.

PB 99 4B

Light brown sediment with clasts ranging from small to medium in size. The clast shape ranges from sub-angular to sub-rounded. Lineations are abundant throughout the sample. A few water escape structures can also be seen.

MB 0610 2008-4B

Dark clay matrix. Generally structure-less with a few small clasts.

Analyse processuelle de Genèse 2:4b-25 : incidences herméneutiques et théologiques

Dans un monde devenu séculier et où la tradition chrétienne est en recul, on est en droit de se poser la question de la pertinence des textes bibliques. En lien avec cette situation, on peut aussi se demander quelle représentation de Dieu émerge de la façon dont on accueille ces textes. Ce type de questionnement sous-tend l’intérêt que peut représenter une approche processuelle des textes bibliques, approche encore peu connue dans le monde francophone. Celle-ci est-elle en mesure d’apporter une certaine nouveauté dans la vision de Dieu généralement proposée en milieu chrétien ? Pour répondre à cela, il a semblé pertinent de tenter l’exercice à partir d’un texte englobant et fondateur. Genèse 2, connu pour raconter la création d’Adam et Ève, est porteur d’images presque stéréotypées à force d’avoir été lu et remâché. À ce titre, il a paru particulièrement approprié. Mais, avant même d’explorer le texte sous un angle processuel, il s’est avéré indispensable de commencer cette démarche par une traduction personnelle du texte hébreu, à partir et avec l’aide d’une analyse syntaxique et textuelle, dont on verra qu’elles ont ouvert le texte à de nouvelles hypothèses de traduction, de nouvelles nuances, comme autant de pistes à confronter à une théologie processuelle. Enfin, cette analyse ne peut se faire sans être en dialogue avec différents commentaires, exégétiques ou non, afin de souligner les convergences comme les divergences rencontrées au fil de la recherche et de la réflexion.

Metal cluster expansion by addition of low-valent group 4B reagents: crystal and molecular structure of [Os3SnH2(CO)10{CH(SiMe3)2}2]; a compound with hydrogen bridging osmium and tin

Cluster expansion of [Os3H2(CO)10] with [SnR2][R = CH(SiMe3)2] take place in high yield to give [Os3SnH2(CO)10R2], the first closed triosmium–main-group metal cluster to be structurally characterized; a novel feature is the presence of a hydrogen atom bridging the tin atom and one of the osmium atoms.

Surface Corrosion of Ti-16Si-4B Powder Alloy Implanted With Nitrogen by Plasma-Based Technique

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

The first report of the human platelet alloantigen 4b allele in a Brazilian

The human platelet alloantigen (HPA) 4b allele is rarely observed in Caucasians and the observed incidence in Asians is usually lower than 1.0%. We report the first Brazilian with the allele HPA-4b, and were able to determined that he inherited it from his father.

Modification of surface properties of Ti-16Si-4B powder alloy by plasma immersion ion implantation

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)