Dual C3a/C5a receptor agonism by a C5a C-terminal analogue decapeptide

No abstract

Bioethanol production by Saccharomyces cerevisiae, Pichia stipitis and Zymomonas mobilis from delignified coconut fibre mature and lignin extraction according to biorefinery concept

In search to increase the offer of liquid, clean, renewable and sustainable energy in the world energy matrix, the use of lignocellulosic materials (LCMs) for bioethanol production arises as a valuable alternative. The objective of this work was to analyze and compare the performance of Saccharomyces cerevisiae, Pichia stipitis and Zymomonas mobilis in the production of bioethanol from coconut fibre mature (CFM) using different strategies: simultaneous saccharification and fermentation (SSF) and semi-simultaneous saccharification and fermentation (SSSF). The CFM was pretreated by hydrothermal pretreatment catalyzed with sodium hydroxide (HPCSH). The pretreated CFM was characterized by X-ray diffractometry and SEM, and the lignin recovered in the liquid phase by FTIR and TGA. After the HPCSH pretreatment (2.5% (v/v) sodium hydroxide at 180 °C for 30 min), the cellulose content was 56.44%, while the hemicellulose and lignin were reduced 69.04% and 89.13%, respectively. Following pretreatment, the obtained cellulosic fraction was submitted to SSF and SSSF. Pichia stipitis allowed for the highest ethanol yield 90.18% in SSSF, 91.17% and 91.03% were obtained with Saccharomyces cerevisiae and Zymomonas mobilis, respectively. It may be concluded that the selection of the most efficient microorganism for the obtention of high bioethanol production yields from cellulose pretreated by HPCSH depends on the operational strategy used and this pretreatment is an interesting alternative for add value of coconut fibre mature compounds (lignin, phenolics) being in accordance with the biorefinery concept.

A un verre ; Béranger au Perreux / [signé A. Debellocq]. Le poète / [signé E. Debellocq fils]

Comprend : Le poète

Retrats d'acollida : Una aproximació des de l'etnografia visual a les cuidadores i cuidadors de la Fundació Pere Mitjans

Acostament etnogràfic visual a un grup de cuidadors/es de la Fundació Pere Mitjans. Descobriment de l'empatia d'un col·lectiu divers de cuidadors/es amb el grup d'usuaris amb l'etiqueta de diferents, per la seva discapacitat o diversitat funcional.

Un colegio especial para una vida normal.

El proyecto propone el establecimiento de una metodología por centros de trabajo que permita al alumnado de Educación Especial un aprendizaje basado en experiencias reales y vivencias que redunden en una mayor autonomía y por lo tanto en un mayor nivel madurativo. Los objetivos son: desarrollar la autonomía personal, tanto individual como social; lograr una conexión real entre la experiencia del alumno en el centro y la de su propio hogar; preparar a los alumnos para la realización de tareas referidas a hábitos de cuidado e higiene personal; y adaptar el desarrollo curricular a su entorno inmediato para facilitar aprendizajes que sirvan para la vida. La experiencia se organiza en torno a cuatro talleres: jardín, cocina, limpieza, cuidado del hogar y aseo personal, y relaciones con el exterior, en los que se aplican aspectos de las diferentes áreas curriculares con carácter práctico e interdisciplinar. Se realizan actividades como manipulación de alimentos; elaboración de recetas; reconocimiento de objetos y su utilidad y necesidad en la vida; dramatización de lo experimentado en el aula; prácticas de higiene del hogar y personal; representación de roles; cuidado del jardín, etc. La valoración de la experiencia destaca que los objetivos se han alcanzado gracias a la coordinación entre el profesorado que lleva a cabo una enseñanza individualizada y a la colaboración de los padres. Se incluye en el proyecto la programación elaborada para los talleres.

Purification of a PHA-Like chitin-binding protein from Acacia farnesiana seeds: A time-dependent oligomerization protein

A lectin-like protein from the seeds of Acacia farnesiana was isolated from the albumin fraction, characterized, and sequenced by tandem mass spectrometry. The albumin fraction was extracted with 0.5 M NaCl, and the lectin-like protein of A. farnesiana (AFAL) was purified by ion-exchange chromatography (Mono-Q) followed by chromatofocusing. AFAL agglutinated rabbit erythrocytes and did not agglutinate human ABO erythrocytes either native or treated with proteolytic enzymes. In sodium dodecyl sulfate gel electrophoresis under reducing and nonreducing conditions, AFAL separated into two bands with a subunit molecular mass of 35 and 50 kDa. The homogeneity of purified protein was confirmed by chromatofocusing with a pI=4.0+/-0.5. Molecular exclusion chromatography confirmed time-dependent oligomerization in AFAL, in accordance with mass spectrometry analysis, which confers an alteration in AFAL affinity for chitin. The protein sequence was obtained by a liquid chromatography quadrupole time-of-flight experiment and showed that AFAL has 68% and 63% sequence similarity with lectins of Phaseolus vulgaris and Dolichos biflorus, respectively.

The Effects of Oxybutynin on Urinary Symptoms in Children with Williams-Beuren Syndrome

Purpose: Williams-Beuren syndrome is a genomic disorder caused by a hemizygous contiguous gene deletion on chromosome 7q11.23. Lower urinary tract symptoms are common in children with Williams-Beuren syndrome. However, there are few data on the management of voiding symptoms in this population. We report our experience using oxybutynin to treat urinary symptoms in children with Williams-Beuren syndrome. Materials and Methods: We prospectively analyzed 42 patients with Williams-Beuren syndrome and significant lower urinary tract symptoms due to detrusor overactivity diagnosed on urodynamics in a 12-week, open-label study. Urological assessment included symptomatic evaluation, the impact of lower urinary tract symptoms on quality of life, frequency-volume chart, urodynamics and urinary tract sonography. After 12 weeks of treatment with 0.6 mg/kg oxybutynin per day given in 3 daily doses, patients were assessed for treatment efficacy and side effects. Results: A total of 17 girls and 19 boys completed medical therapy and were assessed at 12 weeks. Mean +/- SD patient age was 9.2 +/- 4.3 years (range 3 to 18). The most common urinary complaint was urgency, which occurred in 31 patients (86.1%), followed by urge incontinence, which was seen in 29 (80.5%). Compared to baseline, urinary symptoms were substantially improved. The negative impact of storage symptoms on quality of life was significantly decreased from a mean +/- SD of 3.3 +/- 1.7 to 0.5 +/- 0.9 (p <0.001). Mean +/- SD maximum urinary flow improved from 14.2 +/- 15.0 to 20.5 +/- 6.4 ml per second (p <0.001). Conclusions: A total of 12 weeks of therapy with 0.6 mg/kg oxybutynin daily resulted in improvement of lower urinary tract symptoms, quality of life and maximum flow rate in most patients with Williams-Beuren syndrome.

Abbildung was massen das Schiff Hollandia, Utrecht, und die Jacht das Posthorn auff ihrer heimfahrt nach Holland mit den Duinkirchern gestritten

Seegefecht vor Dünkirchen, um 1670