Influência da paridade, do tipo de parto e do nível de atividade física sobre a musculatura do assoalho pélvico em mulheres na pós-menopausa

The post-menopause stage is characterized by hormonal and organic alterations of ovarian failure. One of the most important of these is muscles alterations of the pelvic floor (MPF). According to current literature, in young women gynecological and obstetric factors, as well as lifestyles and habits influence that loss of function. However, there is still uncertainty about the influence of those variables in the MPF functions in post-menopause women. Thus, this study aimed at seeing if there is an influence from number of births, the type of birth and the level of physical activity on the MPF of post-menopause women. Another objective of this study was to compare MPF force in women who had had vaginal births with those who had been subjected to cesarean sections, those with different levels of physical activity and those with artificial and natural menopause in the initial and latter stages. Furthermore, the test of muscular force was compared to perineometry. Using observational, analytical and transversal observations, 100 women in the post-menopausal stage of life, between the ages of 45 and 65, were examined. They were divided according to the menopausal stage into three groups: women who had undergone hysterectomies, those in the initial stages of postmenopause and those in the late stage of postmenpause. The patients were questioned about social, demographic, gynecological and obstetric factors. All the volunteers were submitted to a physical examination where their height and weight were measured to arrive at the corporal mass index and their waist measurements were taken. The evaluation of the pelvic floor was conducted with muscular force tests and perineometry. These results were analyzed with statistical description and ANOVA statistical tests, multiple regression and Kolmogorov-Smirnov evaluations. The results showed homogeneity with regard to social demographic and anthropometric characteristics among the women in the final test sample (n=85). It was also seen that most of the women in all three groups were married (p=0.51) and catholic (p=0.13). The average per capital income varied between $R585.47 (+/-466.67) and $R1,271.83 (+/-1,748.95), with no significant difference between the groups (p=0.05). The G>6 group presented an average age between 58.95 (+/-3.96) which was significantly greater that the G<6 group´s average age (53.21+/- 3.88) (p=0.000). There was no difference between the groups´ anthropometric characteristics of weight (p=0.32), height (p=0.72) and corporal mass index (p=0.34), nor in the waist measurements (p=0.33). Furthermore, no significant difference was noted in the MPF function of women who had had normal births, cesarean sections or a combination of the two (TFM p=0.897; perineum measurement p=0.502). Likewise, no differences were seen in the MPF function of women who had one, two to three or four or more births (TFM p=0.28, perineum measurement p=0.13). Finally, no difference was perceived among those with different levels of physical activity (TFM p=0.663; perineum measurement p=0.741). Therefore, we found that the type of delivery, number of births and physical activity had no influence on the muscular function of the pelvic floor among the women studied. It is believed that decline in muscular function in post-menopause women is fundamentally related to the process of aging.

Does a mandibular RDP and new maxillary CD improve masticatory efficiency and quality of life in patients with a mandibular Kennedy class I arch?

Objectives To evaluate the change in masticatory efficiency and quality of life of patients treated with mandibular Kennedy class I removable partial dentures (RPDs) and maxillary complete dentures at the Department of Dentistry of the Federal University of Rio Grande do Norte. Materials and methods A total of 33 Kennedy class I patients were rehabilitated with maxillary complete dentures, and mandibular RPDs were selected for this non-randomized prospective intervention study. The patients had a mean age of 59.1 years. Masticatory efficiency was evaluated by colorimetric assay using fuchsin capsules. The measurements were conducted at baseline and 2 and 6 months after prosthesis insertion. Quality of life was evaluated using the Oral Health Impact Profile (OHIP-14) at baseline and 6 months after denture insertion. The Kolmogorov-Smirnov normality test was applied. Masticatory efficiency was evaluated by repeated measures ANOVA. Oral health-related quality of life was compared using the paired t test. Results There was no statistically significant difference in masticatory efficiency after denture insertion (p = 0.101). Significant differences were found (p = 0.010) for oral health-related quality of life. A significant improvement in psychological discomfort (p < 0.01) and psychological disability (p < 0.01) was observed. Mean difference value (95 % confidence interval) was 6.8 (3.8 to 9.7) points, reflecting a low impact of oral health on quality of life, considering the 0–56 range of variation of the OHIP-14 and a Cohen’s d of 1.13. Conclusion According to the results of the present study, rehabilitation with Kennedy class I RPDs and complete dentures did not influence masticatory efficiency but improved oral health-related quality of life. Clinical relevance The association between the patient’s quality of life and the masticatory efficiency is important for treatment predictability.

An Examination of Lotka’s law in the Field of Library and Information Studies

The purpose of this study was to test Lotka’s law of scientific publication productivity using the methodology outlined by Pao (1985), in the field of Library and Information Studies (LIS). Lotka’s law has been sporadically tested in the field over the past 30+ years, but the results of these studies are inconclusive due to the varying methods employed by the researchers. A data set of 1,856 citations that were found using the ISI Web of Knowledge databases were studied. The values of n and c were calculated to be 2.1 and 0.6418 (64.18%) respectively. The Kolmogorov-Smirnov (K-S) one sample goodness-of-fit test was conducted at the 0.10 level of significance. The Dmax value is 0.022758 and the calculated critical value is 0.026562. It was determined that the null hypothesis stating that there is no difference in the observed distribution of publications and the distribution obtained using Lotka’s and Pao’s procedure could not be rejected. This study finds that literature in the field of library and Information Studies does conform to Lotka’s law with reliable results. As result, Lotka’s law can be used in LIS as a standardized means of measuring author publication productivity which will lead to findings that are comparable on many levels (e.g., department, institution, national). Lotka’s law can be employed as an empirically proven analytical tool to establish publication productivity benchmarks for faculty and faculty librarians. Recommendations for further study include (a) exploring the characteristics of the high and low producers; (b) finding a way to successfully account for collaborative contributions in the formula; and, (c) a detailed study of institutional policies concerning publication productivity and its impact on the appointment, tenure and promotion process of academic librarians.

Musicalidades Quotidianas da Personalidade: estudo sobre o impacto da música na personalidade, comportamentos, emoções e quotidiano de jovens e adultos de língua portuguesa

Objetivos: A música, incomum pela sua ubiquidade e antiguidade, constitui uma das atividades humanas que ocupa um lugar significativo nas diversas culturas e vida diária. Geralmente agradável para grande parte das pessoas, produz numerosos e desmedidos efeitos, na sua maioria positivos para o ser humano. Esta investigação teve como objetivo principal estudar a relação entre alguns dos diferentes géneros musicais e os de traços de personalidade de jovens e de adultos com idades compreendidas entre os 18 e os 38 anos, fluentes em português. A investigação pretendeu descrever as preferências musicais em função da idade, género, estado civil e habilitações literárias; identificar os contextos, os períodos e as atividades mais comuns durante a escuta de música; reconhecer se a música é uma das atividades de lazer mais importantes para o grupo estudado; conhecer quais as razões mais frequentes apontadas pelos sujeitos para ouvir música; estudar a perceção que as pessoas têm sobre a influência da música na violência e no consumo de substâncias; verificar se os sujeitos consideram as preferências musicais como um fator importante e revelador de informações sobre a personalidade; avaliar o impacto e relação das preferências musicais com a personalidade e verificar quais os pares de emoções mais comuns, sentidos durante a escuta musical, e respetiva intensidade. Metodologia: A amostra foi constituída por 320 indivíduos com idades compreendidas entre os 18 e os 38 anos sendo a faixa etária mais comum a que se situa entre os 24 a 29 anos, maioritariamente do sexo feminino, solteiros, de nacionalidade portuguesa, detentores ou a frequentar um curso superior, nas áreas das ciências sociais/serviços ou exatas e tecnológicas. Os participantes aceitaram responder voluntariamente a uma bateria de testes (QCS, EPI, QMQEC e STOMP-PT). Para a caracterização da amostra, determinaram-se frequências absolutas e relativas ou valores médios e desvios-padrão. A normalidade da distribuição das pontuações médias dos instrumentos foi validada com o teste de Kolmogorov-Smirnov com correção de Lilliefors. A consistência interna estudou-se através do Alpha de Cronbach e da fórmula de Kuder Richarson. As diferenças entre grupos foram avaliadas recorrendo a uma ANOVA, a intensidade e magnitude das relações entre variáveis determinou-se através do coeficiente Eta quadrado (e 2) e com o coeficiente de correlação de Pearson avaliou-se a associação entre as variáveis em estudo. Resultados: Observou-se que a música energética é a mais típica dos escalões etários mais jovens, sendo que a rebelde trespassa todas as gerações, revelando os mais velhos também uma forte preferência pela música reflexiva. As escolhas musicais parecem não ser influenciadas pelo sexo e estado civil. Os indivíduos menos escolarizados parecem preferir músicas do tipo energético, excluindo as convencionais enquanto os detentores de maiores habilitações preferem os estilos reflexivos e rebeldes. É em casa, no quarto, ao fim de semana e quando estão sozinhos que os participantes mais ouvem música, constituindo esta a mais frequente atividade de lazer, por ser uma atividade essencial para a existência, não apelando à violência e ao consumo de substâncias e revelando as preferências musicais aspetos da personalidade. Encontraram-se diferenças estatisticamente significativas entre as dimensões da preferência musical e os traços de personalidade, sendo a música energética a que mais se destaca na extroversão e a rebelde no neuroticismo. Não se obtiveram resultados estatisticamente significativos entre os tipos de música e as emoções. Conclusão: Os resultados devem ser vistos a título de ensaio e como introdutórios, seguindo no entanto os referidos na literatura quer ao nível das preferências por idade, sexo e habilitações literárias, quer ao nível do contexto onde é ouvida, quer ainda entre as dimensões de preferência musical e traços de personalidade. / Aims: Music, unusual for its ubiquity and age, is one of the human activities that occupies a significant place in different cultures and daily life. Generally pleasant to most people, produces many and huge positive effects in humans. This investigation aimed to study the relationship between some of the different music preferences and personality types of young and adults aged between 18 and 38 years, fluent in Portuguese. The research intended to describe the musical preferences based on age, gender, marital status and educational attainment; identify contexts, periods and the most common activities during music listening; recognize the music as one of the most important leisure activities for the group studied; know which are the most frequent reasons given by the subjects to listen to music; study the perception that people have about the influence of music on violence and substance use; check whether the subjects consider the musical preferences as a major factor that reveals information about personality; assess the impact and relationship of musical preferences with the personality and see which pairs of most common emotions are felt during music listening, and its respective intensity. Methods: The sample consisted of 320 individuals aged between 18 and 38 years being the most common age group of between 24 to 29 years, mostly female, single, Portuguese, holders or to attend a higher education in the areas of social / services or exact science. Participants voluntarily agreed to answer a battery of tests (QCS, EPI, QMQEC and STOMP-PT). To characterize the sample, they were determined absolute and relative frequencies and mean values and standard deviations. The normality of the average scores of the instruments has been validated with the Kolmogorov-Smirnov test with Lilliefors correction. Internal consistency was studied using Cronbach's alpha and Kuder Richardson formula. Differences between groups were assessed using an ANOVA, intensity and magnitude of the relationship between variables was determined by Eta squared coefficient and the Pearson correlation coefficient evaluated the association between the study variables. Results: It was observed that the energetic music is the most usual among the younger age groups, and the rebel pierces all generations, revealing the older ones a strong preference for reflective music. The musical choices do not seem to be influenced by sex and marital status. The less educated individuals seems to prefer the energetic type songs, excluding conventional, holders of higher qualifications prefer reflective and rebellious styles. It is at home, in the room, on the weekends and when they are alone that participants listen more music, making this the most common leisure activity, the essential for existence, not calling for violence and substance use and revealing aspects of personality. We found significant differences between the dimensions of music preferences and personality traits, with the energetic music standing out in extraversion and neuroticism on rebel. We did not get significant results among the types of music and emotions. Conclusions: The results should be viewed under test and has introductory , however following the reported in the literature both in terms of preferences by age, sex and education level , both in terms of the context in which it is heard, still follows music preference dimensions and personality traits .

Sensibilidad ética y su relación con el consumo de alcohol en personal de enfermería

Propósito y Método de Estudio: El propósito fue identificar la relación que existe de la Sensibilidad Ética y el consumo de alcohol en el personal de enfermería de un hospital de salud pública del área metropolitana de Monterrey, Nuevo León. El diseño del estudio fue descriptivo correlacional. La población estuvo conformada por 325 enfermeros de ambos sexos de una institución de salud pública en el área metropolitana de Monterrey, Nuevo León. Se utilizó un muestreo aleatorio simple y el tamaño de la muestra se determinó a través del paquete estadístico n’Query Advisor Versión 4.0 ® (Elashoff, Dixon, Crede y Fothenringham, 1997). Se estimó la muestra para una proporción con un intervalo de confianza (IC) de 95%, usando un enfoque conservador (p=q=½) con un límite de error de estimación de .05 (±5%). Se determinó, una muestra final de 219 enfermeros. Se utilizó una Cédula de Datos Personales y Laborales y de Prevalencia de Consumo de Alcohol y los instrumentos: Cuestionario de Sensibilidad Ética [MSQ] que obtuvo un Coeficiente de Confiabilidad de .73 y el Test de Identificación de Trastornos Asociados al Consumo del Alcohol [AUDIT] que presentó una consistencia interna de .80. Se aplicó la prueba de Kolmogorov-Smirnov con corrección de Lilliefors y se utilizó estadística no paramétrica: U de Mann-Whitney, H de Kruskal-Wallis y Coeficiente de Correlación de Spearman. Contribución y Conclusiones: La edad promedio del consumo de alcohol fue de 18 años con un consumo de 3 bebidas alcohólicas en un día típico. La prevalencia global del consumo de alcohol fue de 84.5% con un IC 95% [79-89], la prevalencia lápsica del 70.3% con un IC 95% [64-76], la prevalencia actual fue del 50.2% con un IC 95% [43-56] y la prevalencia instantánea fue del 22.7% con un IC 95% [17-28]. El consumo de alcohol mostró diferencias significativas por sexo (X2=28.6, p=.001) el sexo femenino presentó mayor consumo sensato (64.9%) y el consumo dependiente y dañino fue mayor para el sexo masculino (42.1%, 35.1%). Así mismo se encontraron diferencias significativas de consumo de alcohol por edad (X2=12.47, p=.014), donde le personal de 35 años o más presentó mayor consumo sensato (58.8%) y dañino (18.8%) y el consumo dependiente fue mayor para el grupo de 25 a 34 años (40.4%).

Body composition by dual X-ray absorptiometry in Mexican schoolchildren with or without obesity

Objective: Apply dual X-ray absorptiometry (DXA) to determine the amount of fat mass, lean mass, and bone mineral density in Mexican schoolchildren with and without obesity. Material and methods: We performed an observational, analytical, comparative, cross-sectional study of 80 Mexican schoolchildren who attended the Nutrition Clinic of the Pediatric Medical Center in Monterrey, Mexico during the period of January to April 2005. Body mass index (BMI) was determined to classify the participants according to the growth charts of the Centers for Disease Control and Prevention. Two groups of 40 children each (with and without obesity) were formed and DXA was carried out on each individual. Cronbach’s Alpha was used to determine instrument reliability and the Kolmogorov-Smirnov test was used to test the normality of numerical variables. Means were compared using Student´s t test. Results: Statistically signiicant differences were found in fat mass (p≤0.001) and lean mass (p≤0.001), but not in bone mineral content (p=0.051) between both groups. Conclusions: Differences exist in fat mass and lean mass in both groups, but not in bone mineral content between both groups. A signiicant positive correlation was found between fat mass, determined by DXA, and BMI in schoolchildren with and without obesity

Imagery : correlação entre o motor imagery e as habilidades técnicas de condução de bola no futebol

Dissertação apresentada à Escola Superior de Educação do Instituto Politécnico de Castelo Branco para cumprimento dos requisitos necessários à obtenção do grau de Mestre em Atividade Física.

Size selectivity of trammel nets in southern European small-scale fisheries

Trammel net size selectivity was studied for the most important metiers in four southern European areas: the Cantabrian Sea (Atlantic, Basque Country, Spain), the Algarve (Atlantic, southern Portugal), the Gulf of Cadiz (Atlantic, Spain) and the Cyclades Islands (Mediterranean, Aegean Sea, Greece). These metiers were: cuttlefish (Sepia officinalis) and soles (Solea senegalensis, Microchirus azevia, Synaptura lusitanica) in the Algarve and the Gulf of Cadiz, sole (Solea solea) in the Cantabrian Sea and mixed fin-fish in the Cyclades. In each area, experimental trammel nets of six different types (combinations of two large outer panel mesh sizes and three small inner panel meshes) were constructed. Fishing trials were carried out on a seasonal basis (four seasons in the Cantabrian Sea, Algarve and Cyclades and two seasons in the Gulf of Cadiz) with chartered commercial fishing vessels. Overall, size selectivity was estimated for 17 out of 28 species for which sufficient data were available. Trammel nets generally caught a wide size range of the most important species, with length frequency distributions that were skewed to the right and/or bi-modal. In many cases the length frequency distributions of the different nets were highly overlapped. The Kolmogorov-Smirnov test also showed that the large outer panel meshes generally had no effect in terms of size selectivity, while the opposite was true for the small inner panel ones. Six different selectivity models (normal scale, normal location, gamma, log-normal, bi-modal and gamma semi-Wileman) were fitted to data for the most abundant species in the four areas. For fish, the bi-modal model provided the best fits for the majority of the data sets, with the uni-modal models giving poor fits in most cases. For Sepia officinalis, where trammelling or pocketing was the method of capture in 100% of the cases, the logistic model fitted by maximum likelihood was judged to be more appropriate for describing the size selective properties of the trammel nets. Our results, which are among the first ones on trammel net selectivity in European waters, will be useful for evaluating the impacts of competing gear for the socio-economically important small-scale static gear fisheries. (c) 2006 Elsevier B.V All rights reserved.

Relación entre las condiciones de higiene y seguridad y la exposición ambiental a solventes orgánicos en pintores de automóviles de la localidad de barrios unidos, Bogotá 2015

Introducción. Los trabajadores de los talleres de reparación de automóviles conviven diariamente con la exposición a los solventes orgánicos, exposición que se convierte en un riesgo para su salud que generalmente en el corto plazo se presenta como déficits de concentración, memoria y tiempo de reacción y en el largo plazo produciendo graves repercusiones clínicas como efectos mutagénicos y carcinogénicos. Objetivo. Caracterizar las condiciones higiénicas y de seguridad de trabajadores ocupacionalmente expuestos a solventes orgánicos y determinar los niveles ambientales de benceno, tolueno y xileno (BTX) en talleres de lámina y pintura automotriz de la ciudad de Bogotá. Materiales y métodos. Se hizo un estudio de corte transversal en 60 trabajadores que laboran expuestos a solventes orgánicos en talleres de reparación automotriz en Bogotá. Se realizó una encuesta con variables sociodemográficas, laborales y se determinaron los niveles en aire de benceno, tolueno y xileno. Para los muestreos ambientales, las bombas se colocaron en una posición fija representativa del ambiente general, con el objeto de conocer la distribución de los solventes en el área de trabajo. Se realizó un análisis descriptivo por conteos de frecuencia, medidas de tendencia central y dispersión. Se utilizó prueba de bondad de ajuste para distribución normal (Kolmogorov-Smirnov o Shapiro Wilk), prueba t Student para comparación de medias, o en su defecto prueba U de Mann Whitney para comparación de medianas. Para identificar la relación entre las características sociodemográficas y ocupacionales con la exposición a BTX, se utilizaron pruebas de asociación Chi cuadrado o análisis de correlación según la naturaleza de las variables. El nivel de significancia para cada prueba fue 0.05. Resultados. La edad promedio de los trabajadores fue de 43 años y un tiempo total de exposición a solventes de 20 años. Respecto al uso de protección corporal, 45 (75%) de los trabajadores manifestaron que usaban uniforme, mientras que 14 (23,3%) usaban ropa de calle durante la jornada laboral. El 46,7% manifestaron usar protección respiratoria. La concentración de benceno en aire fue entre 0,1 y 0,45 mg/l (mediana de 0,31 mg/l; DE 0,13 mg/l); la de tolueno estuvo entre 8,25 y 27,22 mg/l (mediana de 14,5 mg/l; DE 6.99 mg/l) y la de xileno entre 19,34 y 150,15 mg/l (mediana de 70,12 mg/l; DE 40,82 mg/l). Conclusión. Los pintores de automóviles están expuestos a niveles elevados de solventes en los lugares de trabajo y no cuentan con condiciones de higiene y seguridad industrial adecuados. Un gran número de pintores son informales lo que les impide el acceso a los beneficios del Sistema de Seguridad Social Integral.

Estimation of climatological water deficit in an experimental watershed in the brazilian cerrado.

ABSTRACT: This study aimed to estimate the probability of climatological water deficit in an experimental watershed in the Cerrado biome, located in the central plateau of Brazil. For that, it was used a time series of 31 years (1982?2012). The probable climatological water deficit was calculated by the difference between rainfall and probable reference evapotranspiration, on a decennial scale. The reference evapotranspiration (ET0) was estimated by the standard FAO-56 Penman-Monteith method. To estimate water deficit, it was used gamma distribution, time series of rainfall and reference evapotranspiration. The adherence of the estimated probabilities to the observed data was verified by the Kolmogorov-Smirnov nonparametric test, with significance level (a-0.05), which presented a good adjustment to the distribution models. It was observed a climatological water deficit, in greater or lesser intensity, between the annual decennials 2 and 32.

Survey regional sobre crescimento e desenvolvimento : relações com maturação, sobrepeso e obesidade, nível socioeconômico e desempenho motor

Universidade Estadual de Campinas . Faculdade de Educação Física

Tracking dos indicadores da aptidão fisica relacionada a saude em escolares

Universidade Estadual de Campinas . Faculdade de Educação Física

Caracterização e distribuição de cronotipos no sul do Brasil: diferenças de gênero e estação de nascimento

OBJETIVO: Investigar a tipologia circadiana e as diferenças de gênero em universitários do sul do Brasil. MÉTODOS: Voluntários (736) de 17 a 49 anos preencheram a versão brasileira do Questionário de Cronotipo (QC), tradução do Morningness-eveningness Questionnaire (MEQ) de Horne e Östberg. Medidas de tendência central e dispersão e curva de distribuição dos escores do QC (Kolmogorov-Smirnov) foram calculadas de acordo com gênero (teste t de Student), idade, estação de nascimento e desconforto com o horário de verão (qui-quadrado). RESULTADOS: Foram incluídos 648 indivíduos (36% homens, 64% mulheres), com perdas de 12% por questionários incorretos. A distribuição dos escores do QC evidenciou uma curva normal (amplitude = 18-77; média = 46,6; desvio-padrão = 10,8). Nesta amostra, 32% foram vespertinos, 54% intermediários e 14% matutinos. As médias do QC foram significativamente diferentes (p = 0,003): homens (44,9 ± 10,8) comparados com mulheres (47,5 ± 10,7) e 70% dos que nasceram na primavera e no verão foram vespertinos (p = 0,015), sem associação gênero-estação do ano. CONCLUSÃO: Homens e nascidos na primavera-verão evidenciaram preferência pela vespertinidade, não havendo diferença de gênero com relação à estação de nascimento. Nossos resultados estão de acordo com estudos realizados no hemisfério norte que mostraram, também, uma associação entre a estação de nascimento e o cronotipo.

Evaluation of IGF-2/ApaI polymorphism in pregnant women infected with human immunodeficiency virus type 1 taking antiretroviral drugs

Objective: Studies carried Out to assess the effects of antiretroviral drugs (ARV) in HIV-1 infected pregnant women have demonstrated carbohydrate intolerance. Some reports also refer to the effect of disturbances in the expression of the insulin-like growth factor (IGF) system on pancreas beta-cell function in humans and IGF-2/ApaI polymorphisms have been associated with obesity and features of the metabolic syndromes. in the present study, we tested the association between IGF-2/ApaI genotype and hyperglycemia in HIV-1 infected pregnant women receiving ARV. Design: We studied IGF-2/ApaI polymorphism in 87 healthy pregnant women, 43 HIV-1 infected pregnant women taking ARV with hyperglycemia during pregnancy, and 43 HIV-1-negative pregnant women with gestational diabetes. Blood samples were obtained for DNA extraction, PCR and genotyping. Data were analyzed statistically by the Kolmogorov-Smirnov normality, ANOVA and chi-square tests. Results: There were no significant differences in genotype frequency among the three groups analyzed. Considering the HIV-1-infected pregnant women, there were no significant differences in genotype frequency between the zidovudine group and the triple antiretroviral treatment group. There were no significant differences in allele frequencies among the groups evaluated. Non-white pregnant women tended to present the GG genotypes compared to white pregnant women. Conclusion: These results contribute to a better understanding of metabolic glycemic disorders in HIV-1 infected pregnant women using ARV, showing that IGF-2/ApaI polymorphisms are not responsible as a single Causative factor of glycemic alterations. These data indicate that other variables should be studied in order to explain these glycemic abnormalities. (C) 2009 Elsevier Ltd. All rights reserved.

Determination of P-glycoprotein, MDR-related protein 1, breast cancer resistance protein, and lung-resistance protein expression in leukemic stem cells of acute myeloid leukemia

Background: The most primitive leukemic precursor in acute myeloid leukemia (AML) is thought to be the leukemic stem cell (LSC), which retains the properties of self-renewal and high proliferative capacity and quiescence of the hematopoietic stem cell. LSC seems to be immunophenotypically distinct and more resistant to chemotherapy than the more committed blasts. Considering that the multidrug resistance (MDR) constitutive expression may be a barrier to therapy in AML, we have investigated whether various MDR transporters were differentially expressed at the protein level by different leukemic subsets. Methods: The relative expression of the drug-efflux pumps P-gp, MRP, LRP, and BCRP was evaluated by mean fluorescence index (MFI) and the Kolmogorov-Smirnov analysis (D values) in five leukemic subpopulations: CD34(+)CD38(-)CD123(+) (LSCs), CD34(+)CD38(+)CD123(-), CD34(+)CD38(+)CD123(+), CD34(+)CD38(+)CD123(-), and CD34(-) mature cells in 26 bone marrow samples of CD34(+) AML cases. Results: The comparison between the two more immature subsets (LSC versus CD34(+)CD38(-)CD123(-) cells) revealed a higher P-gp, MRP, and LRP expression in LSCs. The comparative analysis between LSCs and subsets of intermediate maturation (CD34(+)CD38(+)) demonstrated the higher BCRP expression in the LSCs. In addition, P-gp expression was also significantly higher in the LSC compared to CD34(+)CD38(+)CD123(-) subpopulation. Finally, the comparative analysis between LSC and the most mature subset (CD34(-)) revealed higher MRP and LRP and lower P-gp expression in the LSCs. Conclusions: Considering the cellular heterogeneity of AML, the higher MDR transporters expression at the most immature, self-renewable, and quiescent LSC population reinforces that MDR is one of the mechanisms responsible for treatment failure. (C) 2008 Clinical Cytometry Society.