The reproductive cycle of the male sleep snake Sibynomorphus mikanii (Schlegel, 1837) from southeastern brazil

This study describes the male reproductive cycle of Sibynomorphus mikanii from southeastern Brazil considering macroscopic and microscopic variables. Spermatogenesis occurs during spring-summer (September-December) and spermiogenesis or maturation occurs in summer (December-February). The length and width of the kidney, the tubular diameter, and the epithelium height of the sexual segment of the kidney (SSK) are larger in summer-autumn (December-May). Histochemical reaction of the SSK [periodic acid-Schiff (PAS) and bromophenol blue (BB)] shows stronger results during summer-autumn, indicating an increase in the secretory activity of the granules. Testicular regression is observed in autumn and early winter (March-June) when a peak in the width of the ductus deferens occurs. The distal ductus deferens as well as the ampulla ductus deferentis exhibit secretory activities with positive reaction for PAS and BB. These results suggest that this secretion may nourish the spermatozoa while they are being stored in the ductus deferens. The increase in the Leydig cell nuclear diameter in association with SSK hypertrophy and the presence of sperm in the female indicate that the mating season occurs in autumn when testes begin to decrease their activity. The peak activity of Leydig cells and SSK exhibits an associated pattern with the mating season. However, spermatogenesis is dissociated of the copulation characterizing a complex reproductive cycle. At the individual level, S. mikanii males present a continuous cyclical reproductive pattern in the testes and kidneys (SSK), whereas at the populational level the reproductive pattern may be classified as seasonal semisynchronous. © 2012 Wiley Periodicals, Inc.

Structure, histochemistry and ultrastructure of the male reproductive accessory glands in the neotropical flat-faced fruit-eating bat Artibeus planirostris (Chiroptera: Phyllostomidae)

Chiroptera, the second largest mammalian order, presents different reproductive strategies and unique reproductive features. However, there are few reports regarding male reproductive accessory glands (RAGs) in Chiroptera. Thus, the aim of the present study was to characterise the RAGs of the exclusively neotropical bat Artibeus planirostris (Chiroptera: Phyllostomidae) macroscopically, microscopically and ultrastructurally. The RAGs were composed of a prostatic complex with two regions (ventral and dorsal) and paraurethral and bulbourethral glands, but no seminal vesicles. The ventral region had an undefined epithelium, with secretory and basal cells, and its secretions were periodic acid-Schiff (PAS) positive. The dorsal region received both deferens ducts, had a columnar pseudostratified epithelium with secretory and basal cells. There were two types of secretions from the dorsal region: one that was basophilic and another that was mixed PAS positive and PAS negative. The paraurethral glands were dispersed in the connective tissue of the urethra, whereas the bulbourethral glands were located in the penile root. Histological and ultrastructural data confirmed the prostatic nature of the ventral and dorsal regions and the holocrine nature of the ventral region, with the latter finding never having been described previously for the prostate gland. Our findings demonstrate the wide discrepancy of RAGs between A. planirostris and other mammals in terms of their composition, structure and morphology. © CSIRO 2013.

Caracterização citogenética de populações de crisopídeos (Neuroptera: Chrysopidae) de ocorrência em Jaboticabal, SP

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Proposição e descrição das espécies do grupo Edessa pallida, Dallas, 1851 (Heteroptera, Pentatomidae)

Pentatomidae é a quarta maior família dentro de Heteroptera sendo composta por 760 gêneros e 4700 espécies. Esta família tem distribuição mundial, mas as subfamílias Cyrtocorinae, iscocephalinae e Edessinae são exclusivas da região Neotropical. Dentre as subfamílias de Pentatomidae, Edessinae é uma das que apresenta o maior número de problemas taxonômicos e nomenclaturais, concentrados basicamente no gênero Edessa. Edessinae é um táxon megadiverso (cerca de 280 espécies conhecidas e mais de 350 desconhecidas da ciência) e formada atualmente por cinco gêneros: Edessa (259 espécies descritas), Olbia (5), Pantochlora (1), Peromatus (7) e Brachystethus (10). O estudo atual de Edessa é feito através da organização de exemplares em grupos de espécies morfologicamente semelhantes. Este trabalho segue esta linha com a proposição do grupo Edessa pallida. Este grupo é baseado em características morfológicas externas como a forma afunilada do corpo, padrão de pontuação do corpo, reticulação das asas, padrão de manchas no abdome, bem como características da genitália dos machos. As espécies incluídas neste grupo são Edessa pallida Dallas, 1851, Edessa inscripta Walker, 1868 e Edessa polymita Distant, 1890, além de cinco espécies novas para ciência. O número total de exemplares examinados neste trabalho foi 70 indivíduos pertencentes a 12 coleções nacionais e estrangeiras. As espécies foram descritas, ilustradas e medidas seguindo um padrão adotado para a família. As espécies já conhecidas tiveram suas distribuições geográficas ampliadas. A sp. nov. 1 foi descrita de exemplares provenientes do Suriname, Guiana Francesa e Brasil; a sp. nov. 2 da Guiana e Brasil; a sp. nov. 3 do Brasil; a sp. nov. 4 da Guiana Francesa; e a sp. nov. 5 da Guiana, Suriname. Guiana Francesa e Brasil. Uma chave de identificação e um mapa com a distribuição geográfica das espécies são apresentados.

Systematics and phylogeny of the Neotropical Pachymenes de Saussure and Santamenes Giordani Soika (Hymenoptera, Vespidae, Eumeninae)

The genera Pachymenes de Saussure and Santamenes Giordani Soika arerevised and the phylogenetic relationships among their species, based on external mor-phology and male genitalia, are presented. The cladistics analysis, using 22 terminalspecies (19 ingroup and 3 outgroup species) and 44 characters, produced a single clado-gram under implied weighting. Both genera were recovered as paraphyletic, althoughttwo major clades were formed and were well supported by the re-sampling analysis.We propose the synonymy of Pachymenes with Santamenes, and the description of twonew species: P. saussurei Grandinete n.sp. and P. riograndensis Grandinete n.sp..Newcombinations are: Pachymenes novarae (de Saussure) n.comb., P. olympicus (Zavattari)n.comb., P. peregrinus (Zavattari) n.comb. and P. santanna (de Saussure) revised combi-nation. We state the synonymy of P. obscurus orellanoides under P. obscurus consuetus,reviewing the status of the latter and raising P. consuetus to species level. Pachymenesorellanae vardyi is synonymized under P. orellanae; P. ghilianii olivaceus, P. ghilianiiavissimus and P. peruanus are proposed as synonyms of P. ghilianii; P. picturatusobscuratus is synonymized under P. laeviventris; P. picturatus nigromaculatus andP. picturatus var . intermedia are synonymized under P. picturatus and P. a t ra var . ornatis-sima get its lectotype designated and proposed as synonym of P. ater.

Descrição de uma nova espécie de Rhodnius (Hemiptera, Reduviidae, Triatominae) da Amazônia

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Central American Temnocerus Thunberg, 1815 (Coleoptera: Rhynchitidae)

Twenty eight species of Temnocerus Thunberg, 1815 are recognized from Central America (Mexico to Panama) with eight previously described species and 20 new species as follows: T. abdominalis (Voss), T. chiapensis n. sp., T. chiriquensis (Sharp), T. confertus (Sharp), T. cyaneus n. sp., T. ellus n. sp., T. giganteus n. sp., T. guatemalenus (Sharp), T. guerrerensis n. sp., T. herediensis n. sp., T. mexicanus n. sp., T. michoacensis n. sp., T. minutus n. sp., T. niger n. sp., T. oaxacensis n. sp., T. obrieni, n. sp., T. oculatus (Sharp), T. potosi n. sp., T. pseudaeratus n. sp., T. pueblensis n. sp., T. pusillus (Sharp), T. regularis (Sharp), T. rostralis n. sp., T. rugosus n. sp., T. salvensis n. sp., T. tamaulipensis n. sp., T. thesaurus (Sharp) and T. yucatensis n. sp. Rhynchites debilis Sharp is placed in synonymy with Temnocerus guatemalenus (Sharp) and Pselaphorhynchites lindae Hamilton is placed in synonymy with Temnocerus regularis (Sharp). A key to species based on external characters and male genitalia is provided as well as digital images, aedeagus drawings, and map distributions.

Accidents by external causes in adolescents: care in sentinel urgency and emergency services in the Brazilian State Capitals-2009

Adolescents are seeking new references and experiences, which may involve attitudes of risk and exposure to accidents and violence from external causes. These events constitute a serious Public Health problem. The scope of this study was to analyze the occurrence of accidents by external causes in adolescents from 10 to 19 years of age attended at sentinel urgency and emergency services in Brazil. Data from the 2009 Surveillance System for Violence and Accidents (VIVA 2009) was analyzed in 74 emergency units in 23 state capitals and the Federal District. The findings revealed that 6,434 adolescents (89.8%) were victims of accidents and 730 (10.2 %) were victims of violence. The main causes of the accidents were falls and traffic accidents, and assaults were predominant in violence. For both accidents and violence, non-white male adolescents were predominant and the events occurred most frequently on the public highways. A marked increase was detected, with hospitalization of victims of violence between 15 and 19 years of age. Understanding the epidemiological reality of external causes among adolescents represents an important tool for health prevention and promotion policies and the culture of peace seeking to reduce morbidity and mortality.

Revision of Nanophareus, a mysterious harvestman genus from Chile, with descriptions of three new species (Opiliones: Laniatores: Gonyleptidae)

The Chilean genus Nanophareus Roewer, 1929 is revised and three new species are described: N. araucanus sp. nov. (type locality: Parque Nacional La Campana, Valparaiso, Chile); N. bipartitus sp. nov. (type locality: Parque Nacional La Campana, Valparaiso, Chile); N. bosqenublado sp. nov. (type locality: Parque Nacional Fray Jorge, Coquimbo, Chile). The type species, N. palpalis Roewer, 1929, is redescribed and a lectotype is designated. A cladistic analysis was performed using these three new species plus N. palpalis and 14 more laniatorid species, and a data matrix of 72 characters: Seven from the ocularium, 22 from the dorsal scutum, one from the venter, one from the chelicera, eight from the pedipalp, 24 from male legs, and nine from male genitalia. Two equally most parsimonious trees were found (L = 210; C.I. = 0.41; R.I. = 0.51). Nanophareus was recovered as nested within a paraphyletic subfamily Pachylinae. The genus Nanophareus was found to be monophyletic based on the following exclusive synapomorphies: An external row of enlarged tubercles inserted among small ones on lateral margin of the dorsal scutum (innapplicable in N. bosqenublado); the ventro-basal margin of pedipalpal tibia curved 90 degrees in lateral view; and retrolateral seta of the pedipalpal tibia with a socket apically bifid (socket and seta longer than pedipalpal tibia length).

A novel microdeletion syndrome at 3q13.31 characterised by developmental delay, postnatal overgrowth, hypoplastic male genitals, and characteristic facial features

Background Congenital deletions affecting 3q11q23 have rarely been reported and only five cases have been molecularly characterised. Genotype. phenotype correlation has been hampered by the variable sizes and breakpoints of the deletions. In this study, 14 novel patients with deletions in 3q11q23 were investigated and compared with 13 previously reported patients. Methods Clinical data were collected from 14 novel patients that had been investigated by high resolution microarray techniques. Molecular investigation and updated clinical information of one cytogenetically previously reported patient were also included. Results The molecular investigation identified deletions in the region 3q12.3q21.3 with different boundaries and variable sizes. The smallest studied deletion was 580 kb, located in 3q13.31. Genotype. phenotype comparison in 24 patients sharing this shortest region of overlapping deletion revealed several common major characteristics including significant developmental delay, muscular hypotonia, a high arched palate, and recognisable facial features including a short philtrum and protruding lips. Abnormal genitalia were found in the majority of males, several having micropenis. Finally, a postnatal growth pattern above the mean was apparent. The 580 kb deleted region includes five RefSeq genes and two of them are strong candidate genes for the developmental delay: DRD3 and ZBTB20. Conclusion A newly recognised 3q13.31 microdeletion syndrome is delineated which is of diagnostic and prognostic value. Furthermore, two genes are suggested to be responsible for the main phenotype.

Silhouettella loricatula (Arachnida, Araneae, Oonopidae): a Haplogyne spider with complex female genitalia

The female genital system of the oonopid Silhouettella loricatula is astonishingly complex. The genital opening is situated medially and leads into an oval receptaculum that is heavily sclerotized except for the ventral half of the posterior wall that appears chitinized only. A large striking sclerite lying in the posterior wall of the uterus externus is attached anteriorly to the receptaculum and continues dorsally into a globular appendix that bears a furrow. The uterus externus shows a peculiar modification in its anterior wall: a paddle-like sclerite with a nail-like posterior process. This sclerite lies opposite to the furrow proceeding in the globular appendix and may serve females to lock the uterus externus by muscle contractions. Massive muscles connect the sclerite with the anterior scutum of the opisthosoma and with two other sclerites that are attached to the receptaculum and serve as attachments for further muscles. Gland cells extend around a pore field of the receptaculum. They produce secretion that encloses spermatozoa in a discrete package (secretory sac) inside the receptaculum. In this way, the mixing of sperm from different males and thus sperm competition may be severely limited or completely prevented. During a copulation in the laboratory the ejection of a secretory sac that most probably contained spermatozoa was observed, indicating sperm dumping in S. loricatula. The ejection of the secretory sac may be caused by female muscle contractions or by male pedipalp movements. The majority of the investigated females have microorganisms in the receptacula that could represent symbionts or infectious agents. The microorganisms can be identified partly as bacteria. They are enclosed in secretion and are always found in the same position inside the receptaculum.

Hyperosmotic stress memory in Arabidopsis is mediated by distinct epigenetically labile sites in the genome and is restricted in the male germline by DNA glycosylase activity

Inducible epigenetic changes in eukaryotes are believed to enable rapid adaptation to environmental fluctuations. We have found distinct regions of the Arabidopsis genome that are susceptible to DNA (de)methylation in response to hyperosmotic stress. The stress-induced epigenetic changes are associated with conditionally heritable adaptive phenotypic stress responses. However, these stress responses are primarily transmitted to the next generation through the female lineage due to widespread DNA glycosylase activity in the male germline, and extensively reset in the absence of stress. Using the CNI1/ATL31 locus as an example, we demonstrate that epigenetically targeted sequences function as distantly-acting control elements of antisense long non-coding RNAs, which in turn regulate targeted gene expression in response to stress. Collectively, our findings reveal that plants use a highly dynamic maternal 'short-term stress memory' with which to respond to adverse external conditions. This transient memory relies on the DNA methylation machinery and associated transcriptional changes to extend the phenotypic plasticity accessible to the immediate offspring.

Combined external-endonasal approach for complete excision of the lacrimal drainage apparatus

Purpose: The purpose of this study was to describe a new surgical technique for the complete excision of the lacrimal drainage apparatus (LDA) that combines external and endoscopic approaches. Methods: This study involved a noncomparative, retrospective chart review of the clinical and pathological findings of four patients presenting with LDA papillomas who underwent a combined open and endonasal excision of the lacrimal system. Results. Of the four patients, three were male. The mean age at referral was 41 years, and all cases were unilateral. Histopathology revealed two transitional cell papillomas, one squamous cell papilloma, and one combined transitional/squamous papilloma. Epiphora and an external lesion were the main complaints at presentation. Nasolacrimal duct obstruction was present in all four patients. Papilloma virus infection was suggested in two cases and was confirmed in the only patient who had recurrence. CT identified a solid enhancing mass in two cases. The surgical approach in all cases was performed with the patient under general anesthetic supplemented with infiltration of local anesthesia with vasoconstriction. The lacrimal sac was exposed as per an external dacryocystorhinostomy with biopsy collection from the lacrimal sac lumen to confirm the diagnosis prior LDA excision. The superior aspect of the LDA was isolated by using lacrimal probes in each canaliculus to stabilized parallel incisions and careful dissection toward the common canaliculus until they met the medial aspect of the lacrimal sac. The sac was then separated from the periosteum from the medial orbital wall, using sharp dissection. Finally, an endoscopic dissection of the lower end of the nasolacrimal duct released the most inferior aspect of the LDA, allowing the surgeon to pull and excise the complete system from the external wound. Conclusions: Extensive LDA papillomas required complete excision of the drainage system to prevent recurrence and/or malignant transformation. The use of a combined approach through an open excision of the superior part of the LDA in conjunction with the direct manipulation of the nasolacrimal duct guided by the nasal endoscope facilitates the complete excision of the system for extensive benign lesions.

Differential moderating effect of locus of control on effect of driving experience in young male and female drivers

Investigations of relationships between the specific personality variable, locus of control (LOC, Rotter, 1966) and driver behaviour or accidents have returned contrasting results. Review suggests dependence on gender or experience characteristics of participants, suggesting these factors interact with LOC to influence driving. Relationships were investigated in terms of influence on the eight driving styles of the Multidimensional Driving Style Inventory (MDSI, Taubman-Ben-Ari, Mikulincer & Gillarth, 2004) in young drivers (18-29 years). Gender and LOC differences in driving styles previously related to accidents were proposed. It was also proposed that driving experience influences driving style, and LOC influences effect of driving experience. Gender differences were found for dissociative, anxious, patient, risky, angry and high velocity styles. Women had more external LOC than men, and driver stress styles increased with more external LOC, but reduced with increased driving experience, but so did patient style. High velocity style increased with experience. Controlling for LOC revealed important gender differences in effect of experience: positive effects for men (reducing angry and high velocity, increasing carefulness) and negative effects for women (increasing angry and higher velocity, reducing carefulness). Findings suggest negative influence of high internal LOC on young men in terms of its interaction with experience.

EVIDENCE OF VALIDITY OF THE LSB-50: CROSS-VALIDATION, FACTORIAL INVARIANCE AND EXTERNAL CRITERION ANALYSIS

The aim of this paper was to obtain evidence of the validity of the LSB-50 (de Rivera & Abuín, 2012), a screening measure of psychopathology, in Argentinean adolescents. The sample consisted of 1002 individuals (49.7% male; 50.3% female) between 12 and 18 years-old (M = 14.98; SD = 1.99). A cross-validation study and factorial invariance studies were performed in samples divided by sex and age to test if a seven-factor structure that corresponds to seven clinical scales (Hypersensitivity, Obsessive-Compulsive, Anxiety, Hostility, Somatization, Depression, and Sleep disturbance) was adequate for the LSB-50. The seven-factor structure proved to be suitable for all the subsamples. Next, the fit of the seven-factor structure was studied simultaneously? in the aforementioned subsamples through hierarchical models that imposed different constrains of equivalency?. Results indicated the invariance of the seven clinical dimensions of the LSB-50. Ordinal alphas showed good internal consistency for all the scales. Finally, the correlations with a diagnostic measure of psychopathology (PAI-A) indicated moderate convergence. It is concluded that the analyses performed provide robust evidence of construct validity for the LSB-50