998 resultados para limpeza de patógenos


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The correct distinguishment of microorganisms involved in the periodontal disease pathogen, it is important in the understanding of its progression and adequate treatment planning. Considering this fact, some molecular methods of identification and quantification were developed and are extremely sensitive and precise in the characterization of different bacteria species. The present study aimed to realize a literature review, including studies that realized a comparative analysis between bacterial culture and real time PCR methods in the identification of pathogens. The bacterial culture method can possibly identify new microorganisms and realize antibiotics sensitivity tests. The real time PCR is a microbiologic test that identifies and quantifies bacterial species, through gene amplification of predetermined DNA fragments, with high sensitivity and specificity, and need a shorter operation time of the operator when compared to the bacterial culture method. In this way, to determine a specific diagnostic test, should be considered not only its precision in the identification of microorganisms, but the cost-benefit relationship as well.

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Introduction: Currently, there are many questions regarding the cleaning methods seeking greater efficiency and less loss of burs. Aim: the aim of this study was to evaluate the influence of cleaning methods on the cutting efficiency and morphological characteristics of stainless steel burs tungsten carbide (carbide). Materials and method: Thirty burs were divided into five groups (n = 5) according with the cleaning method: L1 - steel brush, L2 - nylon brush, L3 - ultrasound  +  distilled water, L4 - ultrasound + descaling solution and L5 - no cleaning method (control). The burs were used for the cutting of bovine enamel during six periods of 12 minutes each. After each period, the burs were cleaned (except L5 ) following the protocol established for each group. The cutting efficiency was determined by mass loss and morphological characteristics. Result: The average amount of wear after 72 minutes of use were L1  = 0.3558 g; L2  = 0.4275 g; L3  = 0.4652 g; L4  = 0.4396 g e L5  = 0.4854 g; significant differences in the time of use (p  <  0.001) and cleaning method (p  <  0.001). The L1 group showed the worst performance. Regardless of the experimental group, morphological analysis revealed alterations in the cutting blades soon after the first 12 minutes, being L1 the most affected group. Conclusion: The cleaning with wire brush was the most damaging method to the cutting efficiency and to the morphology of carbide burs.

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The irrigation of root canals aims to their cleaning and disinfection, improving endodontic treatment success. OBJECTIVE: To investigate the influence of the diameter and type of irrigation needle and the root canal enlargement on the mechanical efficacy of endodontic irrigation. MATERIAL AND METHODS: Twelve human single-rooted mandibular incisors were used. During some instrumentation stages (enlargement by #20, #30, and #40 K file), root canals were filled with radiographic contrast solution mixed to propyleneglycol and zinc oxide. Needles with different diameters and designs were employed: G1 – 23G and lateral opening; G2 – 22G and apical opening; G3 – 30G and lateral opening; G4 – 30G and apical opening. The needles were inserted up to resistance, with 1 mm step-back to avoid root canal obliteration. The irrigation was performed with 2 mL of distilled water. Before and after irrigation/aspiration, teeth were radiographed at bucco-lingual and mesiodistal direction, using a digital radiographic system. Then, root canal areas, before (filled by contrast solution) and after irrigation (remnant of contrast solution), were submitted to image subtraction with Adobe Photoshop CS4 software. Subsequently, the areas were measured by Image Tool 3.0 software, allowing the obtaining of the cleaning percentage for each modality. Data were analysed by using Anova and Tukey's test. The level of significance was set at P < 0.05. RESULTS: For all root canal enlargements, 30G needles (G3 e G4) presented a better cleaning efficacy. In all groups, higher cleaning efficacy percentage was observed at #30 and #40 K file enlargement. CONCLUSION: Regardless their design, thinner needles were more effective; a better cleaning efficacy occurred in more enlarged root canals.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Risk of mechanical injuries contraindicate brushing and elect chemical agents as appropriate for daily cleaning of dentures relined with resilient materials. It was evaluated the effect of denture cleansers on the wettability of denture relining material (Dentusoft, Dental Medrano). We used 20 discs of acrylic resin thermoactivated VipiCril ® with 30mm diameter and 4mm thick, covered by 2mm Dentusoft ®. Divided into 4grupos were stored in artificial saliva at 37 +1 º C for 30 days, immersed daily for 15minutes in distilled water (GI), Corega ® Tabs (GII), a solution of sodium bicarbonate (GIII) or solution of sodium hypochlorite (GIV) after which, on the soft liner were poured 2 ml of type IV gypsum (Durone IV, Dentsply). Reached the final setting of the gypsum specimens were sectioned vertically and medially, settled water with sandpaper No. 400 and mounted on suitable device for reading (in the right and left) of the contact angle Carl Zeiss microscope (precision, 001). The results were submitted to analysis, showed non-normal distribution, opting for non-parametric test. Kruskal Wallis test performed at 5% significance, there was statistical difference between the groups with lower average contact angle for GII. It was concluded that the chemical cleaning Corega Tabs ® allowed a better adaptation of relining the gypsum studied.

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The teaching of acoustics has been characterized by a banking model that little contributes to the ideal training of citizens capable of understanding and acting to improve their environment soundscapes. Equally distant from the world of sound and musical culture, audio technology and acoustic environment, it is disconnected from the ever-increasing effort to raise awareness on hearing and sound education, as defended by the Canadian educator Prof. Raymond Murray Schafer. In order to provide elements for reflection on how Mathematics can be itself a language to compete in a sound education, we developed, in a dialogical and problematizing method applied to the technological and cultural world, one further research and teaching with Math students of UNEMAT in Barra do Bugres. This study pointed to the feasibility of educating consciences capable of of improving their acoustic environment, modifying the landscapes where we live, under our responsibility.

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Las aguas residuales son potenciales portadoras de enteroparásitos patógenos para el hombre cuya dosis infectiva puede ser mínima y prolongado el tiempo de sobrevivencia de huevos y quistes en el ambiente. La reglamentación limita la presencia de huevos de helmintos y, hasta el año 2000, no proponía una técnica para su detección. Este trabajo se realizó para caracterizar parasitológicamente los efluentes procedentes de agroindustrias. La capacitación en la identificación microscópica demandó la confección de fichas que describen las características de huevos de helmintos. Se efectuaron ensayos con muestras de bodegas y aceiteras en los puntos detectados de contaminación. Los métodos fueron adaptados a las particularidades de los efluentes. Los resultados no señalaron presencia de helmintos; no obstante, se hallaron Cyclospora e Isospora, protozoarios que, por su alto poder infectivo, también constituyen un riesgo para el hombre. Sin embargo, no están contemplados por la legislación. Respecto a helmintos, es necesario un estudio comparativo de las técnicas de detección para efluentes agroindustriales, con el fin de establecer las que optimicen su recuperación. En cuanto a otras especies es preciso continuar investigando su presencia para evaluar la conveniencia de incorporarlas a la reglamentación.

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