974 resultados para imunofluorescência direta


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The aim of this work was to evaluate the effect of a millet-soybean rotation, during the implantation phase of no-tillage system on the physical properties of a Nitossolo Vermelho distroférrico and the dry mass production of millet and the productivity of soybean. The experimental design used was a randomized blocks, in a split splot arrangement, with four replications. The parcels was constituted by three sowings (E1, E2 and E3) and the subparcels was constituted by harvests, where each harvest handling was: M1 - harvest each blooming and covering withdrawal; M2 - harvest each blooming and covering permanency; M3 - only in the first harvest on blooming and covering withdrawal; M4 - only in the first harvest on blooming and covering permanency, and M5-free growing, with no harvest. Samples were collected from three soil layers: 0-0,05, 0,05-0,10 and 0,10-0,20 m. The E2 showed smaller values of soil density and larger values of total porosity. The E3 resulted in smaller values of flocculation degree and mean weight diameter of the aggregates. The E3 showed smaller production of dry mass.

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To verify the presence of cross-reaction among leishmaniosis, ehrlichiosis and babesiosis in serological diagnostics used in human visceral leishmaniasis control programs, serum samples from leishmaniasis endemic and non-endemic areas were collected and tested by Indirect Fluorescent Antibody (IFAT) and Enzyme-linked immunosorbent assay (ELISA). All serum samples from endemic areas were positive for Leishmania sp., by ELISA and IFAT, 51% positive for Babesia canis and 43% for Ehrlichia canis by IFAT. None of the serum samples from non-endemic areas were positive for Leishmania sp., by IFAT, but 67% were positive for B. canis and 78% for E. canis using the same test. When tested by ELISA for Leishmania sp., four samples from non-endemic area were positive. These dogs were then located and no clinical signs, parasites or antibody was detected in new tests for a six month period. Only one of these 4 samples was positive for B. canis by IFAT and ELISA and three for E. canis by IFAT. The results of the work suggest a co-infection in the endemic area and no serological cross-reaction among these parasites by IFAT and ELISA.

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Economically, onion is the third more important vegetable in Brazil. As a consequence of the increasing by use of new hybrid varieties, the nutritional management of the crop needs to be adequately known. This research was carried out in São José do Rio Pardo, São Paulo State, Brazil, from March 7 to August 8, 2004. The objective was to quantify, under field conditions, the accumulation of macronutrients by onion cultivars established by direct sowing. The experimental design was in randomized blocks with three replicates. The cultivars studied were Optima and Superex. Samples were taken at 30, 50, 70, 90, 110, 130 and 150 days after sowing. With population of 354,000 plants ha -1 and productivity estimated in 64.8 t ha -1, 'Optima' extracted on 150 days after sowing: 78.77, 13.04, 75.77, 59.09, 11.70 and 24.38 kg ha -1 of N, P, K, Ca, Mg and S, respectively, and 'Superex', for the same plant population, and productivity at 72 t ha -1, 64.68, 10.33, 68.28, 65.58, 13.03 and 24.74 kg ha -1 of N, P, K, Ca, Mg and S, respectively. The Optima hybrid exported through its bulb about 49.17% of N, 60.60% of P, 61.16% of K, 41.15% of Ca, 43.56% of Mg, and 47.18 of S, the equivalent to 38.73, 7.90, 46.34, 24.32, 5.10 and 11.51 kg ha -1, respectively. The Superex hybrid exported through its bulb about 44.29% of N, 55.85% of P, 62.54% of K, 35.71% of Ca, 31.82% of Mg, and 48.80% of S, the equivalent to 28.65, 5.77, 42.70, 23.42, 4.15, and 12.07 kg ha -1, respectively.

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Visceral Leishmaniasis (VL) is a zoonosis caused by Leishmania spp. protozoa. Dog is the main parasite's reservoir, especially in urban areas. Indirect Immunofluorescent Antibody Test (IFAT) is commonly used for canine visceral leishmaniasis (CVL) diagnosis, by detection of antibodies against the parasite. However, Trypanosoma cruzi (T.cruzi) cross reactions can occur in serological diagnosis. In order to evaluate the occurrence of cross reactions between antigens of Leishmania spp. and T.cruzi, 150 blood samples of CVL epidemiological inquiry positive dogs were collected and sera were tested by IFAT. Dogs were culled at Zoonosis Control Center of Bauru (SP), an endemic area for VL. Another 150 dogs' blood samples were collected in Botucatu (SP), a non endemic area for LV, and sera were also tested by IFAT. Serum samples of dogs from Bauru were positive, both for Leishmania spp. and for T. cruzi, showing high cross-reactions incidence, reinforcing the need of diagnosis confirmation by other tests. Serum samples of dogs from Botucatu demonstrated low prevalence of positive results by IFAT, both for Leishmania spp. and T.cruzi. However, despite the low number of positive serological results for these parasites, the epidemiological and serological investigation for LV and Chagas disease in dogs from Botucatu must be kept, considering the importance of dog as domestic reservoir for both parasites.

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This study was carried out to evaluate the effects of surface application of lime rates on soil chemical attributes and soybean yield in a no-tillage system. It was installed a field experiment in a factorial 2×4, and the factors were: two types of lime (calcitic and dolomitic); four rates (0.0 - 0.5 - 1.0 and 1.5 t ha-1 of CaCO3), in a completely randomized blocks experimental design, with four replications. The experiment was located in Matupá, Mato Grosso State, Brazil, being the work developed between November 2006 and May 2007. The evaluations were chemical attributes in the soil layers (0,0-2,5 cm, 2,5-5,0 cm and 5,0-10,0 cm), and the productive characteristics of soybean. Application of lime increased the soil pH (CaCl 2), the exchangeable Ca and Mg, the effective CEC and the base saturation only in the soil surface layer. The two limes were different only for Ca and Mg values in the first layer of soil, and only for the Mg values in the layer of 2,5-5,0 cm. The surface liming in no-tillage system did not change soybean yield.

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In the no till system, soil acidity correction practice is restricted to limestone use and there is little information regarding slag. The study aimed to evaluate the amendments in soil chemical properties, yield and bean nutrient uptake according to the application forms of slags, compared to limestone, in the implantation of no till system. The experiment was conducted in the field at College of Agricultural Sciences, Botucatu (SP) from December 2010 to May2011. The treatments consisted of two application ways of seven soil acidity correctives: steel slag, blast furnace slag, ladle furnace slag, stainless steel slag (agrosilício), wollastonite, lime and calcined dolomite lime, plus one control without corrective application. Each material dose was calculated to raise the base saturation to 70%. Soil acidity was neutralized down to 20cm with limestones, whereas for wollastonite and ladle furnace slag those effects occurred down to 10cm, for steel slag, blast furnace slag and agrosilício the corrective effect was restricted to the first 5cm. The bean yield increased by application of correctives in soil acidity, without differences between the application ways.

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Statistical equations were obtained and evaluated with annual, seasonal and monthly data groupings for estimates of direct and diffuse components of solar radiation based on the sunshine duration (ratio of sunshine and photoperiod) incident on horizontal and inclined surfaces to 12.85, 22.85 and 32.85° with facing to North, in Botucatu, SP. The ratios between the two components and radiation at the top of the atmosphere were used, in a database whose inclinations were measured in three different periods (22.85°: 04/1998 to 07/2001; 12.85°: 08/2011 to 02/2003; and 32.85°: 03/2003 to 12/2007) and concomitant with horizontal measures and sunshine duration. The correlations showed a linear and second degree polynomial behavior for the direct and diffuse radiation, with higher coefficients of determination in periods of low variation in the coverage of the sky (cloudiness). The highest values of the direct and diffuse radiation were found in winter and summer, respectively for all surfaces evaluated. The increase in the inclination angle decreased the performance of equations in all groups of data with increase in scattering and decrease in index of the adjustment, however, the monthly equations allowed better performance for the two components.

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

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Pós-graduação em Agronomia (Agricultura) - FCA