STUDIES ON THE PATTERNS OF NUCLEOTIDE AND AMINO ACID SUBSTITUTION (ELECTROPHORESIS, MUTATION RATE, SELECTION, BASE, COMPOSITION)

Theoretical and empirical studies were conducted on the pattern of nucleotide and amino acid substitution in evolution, taking into account the effects of mutation at the nucleotide level and purifying selection at the amino acid level. A theoretical model for predicting the evolutionary change in electrophoretic mobility of a protein was also developed by using information on the pattern of amino acid substitution. The specific problems studied and the main results obtained are as follows: (1) Estimation of the pattern of nucleotide substitution in DNA nuclear genomes. The pattern of point mutations and nucleotide substitutions among the four different nucleotides are inferred from the evolutionary changes of pseudogenes and functional genes, respectively. Both patterns are non-random, the rate of change varying considerably with nucleotide pair, and that in both cases transitions occur somewhat more frequently than transversions. In protein evolution, substitution occurs more often between amino acids with similar physico-chemical properties than between dissimilar amino acids. (2) Estimation of the pattern of nucleotide substitution in RNA genomes. The majority of mutations in retroviruses accumulate at the reverse transcription stage. Selection at the amino acid level is very weak, and almost non-existent between synonymous codons. The pattern of mutation is very different from that in DNA genomes. Nevertheless, the pattern of purifying selection at the amino acid level is similar to that in DNA genomes, although selection intensity is much weaker. (3) Evaluation of the determinants of molecular evolutionary rates in protein-coding genes. Based on rates of nucleotide substitution for mammalian genes, the rate of amino acid substitution of a protein is determined by its amino acid composition. The content of glycine is shown to correlate strongly and negatively with the rate of substitution. Empirical formulae, called indices of mutability, are developed in order to predict the rate of molecular evolution of a protein from data on its amino acid sequence. (4) Studies on the evolutionary patterns of electrophoretic mobility of proteins. A theoretical model was constructed that predicts the electric charge of a protein at any given pH and its isoelectric point from data on its primary and quaternary structures. Using this model, the evolutionary change in electrophoretic mobilities of different proteins and the expected amount of electrophoretically hidden genetic variation were studied. In the absence of selection for the pI value, proteins will on the average evolve toward a mildly basic pI. (Abstract shortened with permission of author.) ^

Performance recovery from exercise: A review from a statistical point of view

Background. Research into methods for recovery from fatigue due to exercise is a popular topic among sport medicine, kinesiology and physical therapy. However, both the quantity and quality of studies and a clear solution of recovery are lacking. An analysis of the statistical methods in the existing literature of performance recovery can enhance the quality of research and provide some guidance for future studies. Methods: A literature review was performed using SCOPUS, SPORTDiscus, MEDLINE, CINAHL, Cochrane Library and Science Citation Index Expanded databases to extract the studies related to performance recovery from exercise of human beings. Original studies and their statistical analysis for recovery methods including Active Recovery, Cryotherapy/Contrast Therapy, Massage Therapy, Diet/Ergogenics, and Rehydration were examined. Results: The review produces a Research Design and Statistical Method Analysis Summary. Conclusion: Research design and statistical methods can be improved by using the guideline from the Research Design and Statistical Method Analysis Summary. This summary table lists the potential issues and suggested solutions, such as, sample size calculation, sports specific and research design issues consideration, population and measure markers selection, statistical methods for different analytical requirements, equality of variance and normality of data, post hoc analyses and effect size calculation.^

Selection of body fat distribution indices in adults and associations with plasma lipid metabolism variables

Body fat distribution is a cardiovascular health risk factor in adults. Body fat distribution can be measured through various methods including anthropometry. It is not clear which anthropometric index is suitable for epidemiologic studies of fat distribution and cardiovascular disease. The purpose of the present study was to select a measure of body fat distribution from among a series of indices (those traditionally used in the literature and others constructed from the analysis) that is most highly correlated with lipid-related variables and is independent of overall fatness. Subjects were Mexican-American men and women (N = 1004) from a study of gallbladder disease in Starr County, Texas. Multivariate associations were sought between lipid profile measures (lipids, lipoproteins, and apolipoproteins) and two sets of anthropometric variables (4 circumferences and 6 skinfolds). This was done to assess the association between lipid-related measures and the two sets of anthropometric variables and guide the construction of indices.^ Two indices emerged from the analysis that seemed to be highly correlated with lipid profile measures independent of obesity. These indices are: 2*arm circumference-thigh skinfold in pre- and post-menopausal women and arm/thigh circumference ratio in men. Next, using the sum of all skinfolds to represent obesity and the selected body fat distribution indices, the following hypotheses were tested: (1) state of obesity and centrally/upper distributed body fat are equally predictive of lipids, lipoproteins and apolipoproteins, and (2) the correlation among the lipid-related measures is not altered by obesity and body fat distribution.^ With respect to the first hypothesis, the present study found that most lipids, lipoproteins and apolipoproteins were significantly associated with both overall fatness and anatomical location of body fat in both sex and menopausal groups. However, within men and post-menopausal women, certain lipid profile measures (triglyceride and HDLT among post-menopausal women and apos C-II, CIII, and E among men) had substantially higher correlation with body fat distribution as compared with overall fatness.^ With respect to the second hypothesis, both obesity and body fat distribution were found to alter the association among plasma lipid variables in men and women. There was a suggestion from the data that the pattern of correlations among men and post-menopausal women are more comparable. Among men correlations involving apo A-I, HDLT, and HDL$\sb2$ seemed greatly influenced by obesity, and A-II by fat distribution; among post-menopausal women correlations involving apos A-I and A-II were highly affected by the location of body fat.^ Thus, these data point out that not only can obesity and fat distribution affect levels of single measures, they also can markedly influence the pattern of relationship among measures. The fact that such changes are seen for both obesity and fat distribution is significant, since the indices employed were chosen because they were independent of one another. ^

Diet selection by the lesser rhea (Rhea pennata pennata) in Payunia, Northern Patagonia (Mendoza, Argentina)

The lesser rhea (family Rheidae) is a flightless large bird of South America, threatened due to habitat loss, hunting and egg collecting, with special concern in Northern Patagonia. Diet and food availability were estimated throughout the year by micro-histological analysis and point-quadrat transects in a landscape inside and another outside the Payunia Reserve, the northernmost part of the Rhea pennata pennata distribution. Significant differences were detected by Kruskall-Wallis ANOVA, food selection by Chi-square test and Bailey’s confidence interval. A strong food selection characterized the diet of lesser rheas, dominated by leaves of shrubs and forbs, complemented by dicot seeds and a few insects. This agrees with the documented low dietary overlap with other herbivores in Payunia. Dietary changes agree with the expected from the selective quality hypothesis. Food availability was better inside than outside the protected area, with probable conservation effects for lesser rheas. Seeds, forbs and soft grasses could be for lesser rheas some key food resources to survive during unfavorable seasons in arid environments without "mallines", as Payunia. Shrubby patches, with high availability of preferred food items (tall shrubs and forbs), stood out as key habitats. Therefore, avoiding fire and woody plant removal is crucial for the conservation of lesser rheas in the northern of its range.

Ocean acidification impacts on sperm mitochondrial membrane potential bring sperm swimming behaviour near its tipping point

Broadcast spawning marine invertebrates are susceptible to environmental stressors such as climate change, as their reproduction depends on the successful meeting and fertilization of gametes in the water column. Under near-future scenarios of ocean acidification, the swimming behaviour of marine invertebrate sperm is altered. We tested whether this was due to changes in sperm mitochondrial activity by investigating the effects of ocean acidification on sperm metabolism and swimming behaviour in the sea urchin Centrostephanus rodgersii. We used a fluorescent molecular probe (JC-1) and flow cytometry to visualize mitochondrial activity (measured as change in mitochondrial membrane potential, MMP). Sperm MMP was significantly reduced in delta pH -0.3 (35% reduction) and delta pH -0.5 (48% reduction) treatments, whereas sperm swimming behaviour was less sensitive with only slight changes (up to 11% decrease) observed overall. There was significant inter-individual variability in responses of sperm swimming behaviour and MMP to acidified seawater. We suggest it is likely that sperm exposed to these changes in pH are close to their tipping point in terms of physiological tolerance to acidity. Importantly, substantial inter-individual variation in responses of sperm swimming to ocean acidification may increase the scope for selection of resilient phenotypes, which, if heritable, could provide a basis for adaptation to future ocean acidification.

Terrain model of the Håkon Mosby Mud Volcano (10 m grid size)

The Håkon Mosby Mud Volcano is a natural laboratory to study geological, geochemical, and ecological processes related to deep-water mud volcanism. High resolution bathymetry of the Håkon Mosby Mud Volcano was recorded during RV Polarstern expedition ARK-XIX/3 utilizing the multibeam system Hydrosweep DS-2. Dense spacing of the survey lines and slow ship speed (5 knots) provided necessary point density to generate a regular 10 m grid. Generalization was applied to preserve and represent morphological structures appropriately. Contour lines were derived showing detailed topography at the centre of the Håkon Mosby Mud Volcano and generalized contours in the vicinity. We provide a brief introduction to the Håkon Mosby Mud Volcano area and describe in detail data recording and processing methods, as well as the morphology of the area. Accuracy assessment was made to evaluate the reliability of a 10 m resolution terrain model. Multibeam sidescan data were recorded along with depth measurements and show reflectivity variations from light grey values at the centre of the Håkon Mosby Mud Volcano to dark grey values (less reflective) at the surrounding moat.

Single-Stage Grid-Connected Forward Microinverter with Boundary Mode Control

This paper presents a microinverter to be integrated into a solar module. The proposed solution combines a forward converter and a constant off-time boundary mode control, providing MPPT capability and unity power factor in a single-stage converter. The transformer structure of the power stage remains as in the classical DC-DC forward converter. Transformer primary windings are utilized for power transfer or demagnetization depending on the grid semi-cycle. Furthermore, bidirectional switches are used on the secondary side allowing direct connection of the inverter to the grid. Design considerations for the proposed solution are provided, regarding the inductance value, transformer turns ratio and frequency variation during a line semi-cycle. The decoupling of the twice the line frequency power pulsation is also discussed, as well as the maximum power point tracking (MPPT) capability. Simulation and experimental results for a 100W prototype are enclosed

Success possibilities of grid parity in particular cases in the new spanish regulatory framework

During the last 10 years the Spanish photovoltaic market has experienced one of the most important increases worldwide. The continuous raise on the price of the electricity in Spain, as in other European countries, USA and Japan, as well as the decrease of the cost of solar photovoltaic systems along this decade is opening a new way to reach grid parity point in some particular scenarios. A new Spanish legislation is being performed toward selfconsumption, and it is in this new context where the grid parity in a wide sense could be achieved. This work will study different cases in Spain, in order to determine whether grid parity would be possible along 2012. Keywords: grid parity, self-consumption, photovoltaic, net-metering

Grid-based histogram arithmetic for the probabilistic analysis of functions

The selection of predefined analytic grids (partitions of the numeric ranges) to represent input and output functions as histograms has been proposed as a mechanism of approximation in order to control the tradeoff between accuracy and computation times in several áreas ranging from simulation to constraint solving. In particular, the application of interval methods for probabilistic function characterization has been shown to have advantages over other methods based on the simulation of random samples. However, standard interval arithmetic has always been used for the computation steps. In this paper, we introduce an alternative approximate arithmetic aimed at controlling the cost of the interval operations. Its distinctive feature is that grids are taken into account by the operators. We apply the technique in the context of probability density functions in order to improve the accuracy of the probability estimates. Results show that this approach has advantages over existing approaches in some particular situations, although computation times tend to increase significantly when analyzing large functions.

Pre-Feasibility Study of Hybrid Wind Power-H-2 System Connected to Electrical Grid

The growth of wind power as an electric energy source is profitable from an environmental point of view and improves the energetic independence of countries with little fossil fuel resources. However, the wind resource randomness poses a great challenge in the management of electric grids. This study raises the possibility of using hydrogen as a mean to damp the variability of the wind resource. Thus, it is proposed the use of all the energy produced by a typical wind farm for hydrogen generation, that will in turn be used after for suitable generation of electric energy according to the operation rules in a liberalized electric market.

Convento dominico de Miguel Fisac en Madrid. El acento de los objetos

La tesis estudia el Convento de Los Dominicos en Madrid (1955-1958) que representa una de las obras más importantes del arquitecto Miguel Fisac (1913-2006). Supone el punto de partida de una etapa profesional donde comienza a cualificar los espacios y a abandonar la idea de la arquitectura como volumen. El proyecto se ubica en un lugar sin apenas referencias (sólo el aire, la luz y el agua) donde Fisac incorpora objetos entendidos a distintas escalas, que acentúan la correcta organización del programa propuesto. Objetos-edificios, objetos-techos de luz y objetos táctiles pertenecen a espacios singulares del conjunto conventual que se enlazan mediante un sistema de patios como estrategia de articulación. En este edificio se encuentran las claves y conceptos que Fisac aplica posteriormente en las obras de esta etapa que acaba de comenzar. Partiendo de la hipótesis de que lo cercano cualifica el espacio, se hace hincapié en los objetos que ponen en valor los lugares más significativos y en las relaciones espaciales que se plantean entre ellos. El objetivo es analizar los recursos espaciales utilizados por Fisac en este proyecto así como la relación con el lugar, la articulación de los espacios y la preocupación por el detalle. La tesis se estructura en tres capítulos: con-texto, pre-texto y texto. El primer capítulo describe el convento, comenzando con una introducción sobre la influencia de los viajes experimentales realizados por Fisac antes del desarrollo del proyecto. Es un estudio desde el punto de vista del autor y con documentación original. El segundo capítulo analiza la obra para identificar aquellos elementos que constituyen la naturaleza del proyecto y su organización espacial enfatizando el sistema de patios como elemento estructurante y una selección de los espacios singulares de la iglesia, el refectorio y las aulas que permiten tratar temás relacionados con la planta convergente en la iglesia, la doble escala en el refectorio y la iluminación en el caso de las aulas. Estos temás se explican con la elaboración de nuevos planos que apoyan el análisis. El tercer capítulo titulado el acento de los objetos, selecciona algunos de estos elementos a distintas escalas: torre, escalera, lucernario y fuente. Con el objetivo de profundizar en cada uno de ellos se utiliza la comparativa con otras referencias que plantean temás similares para entender los conceptos planteados por Fisac y su alcance. A través de la torre y la escalera del claustro, se tratan temás relacionados con la geometría y la trama estructural. Los techos de luz plantean conceptos de iluminación, acústica y continuidad interior y los pequeños objetos de piedra, madera y metal sirven para realizar un acercamiento a la materia y a la técnica a través del detalle, que Fisac aplica en casi todas sus obras. Con la superposición de estas visiones- la del historiador, la del arquitecto y la del crítico- se calibra el grado de elaboración de la obra y se sacan las conclusiones que representan los aspectos más importantes que acomete Fisac en este proyecto. ABSTRACT This thesis studies the Convent for the Dominican Order in Madrid (1955- 1958) which represents one of the most important buildings designed by the Spanish architect Miguel Fisac(1913-2006). This work will become the first step of a professional phase where he begins to qualify the spaces and abandons the idea of Architecture as a Volume. The building is located in a site without almost any reference (only the air, the light and the water). Here, Fisac incorporates different kinds of objects understood at different scales, which emphasizes the correct organisation of the program. Object-buildings, object- ceiling light and tactile-objects belong to singular room of the Conventual complex. These items connect to each other by a system of courtyards used as a strategy for the articulation of the spaces. In this building it is possible to find the keys and concepts that Fisac will apply in following his buildings of this same period that he just has started. Based on the hypothesis that the near things qualify the space, special emphasis is put on the objects that show the great value of the most significant places and the spatial relation that is created between them. The final objective is to analyse the spatial resources used by Fisac in this project, as well as the relation created with the site, the articulation of the spaces and the emphasis on the building details. The thesis is structured around three chapters: con-text, pre-text and text. The first one describes the Seminary, starting with an introduction about the influence of the research trips did by Fisac before the project was development. It is a study from the authors point of view using original documents. The next chapter analyses his work in order to identify those elements that constitute the nature of the project and its spatial organisation with special focus on the system of courtyards as element that structure everything, and a selection of the singular spaces in the church, the refectory and the classrooms that allows to treat issues related to the church´s convergent plan, the double scale of the refectory and the lighting of the classrooms. These issues are explained by new elaborated plans that support the analyse. The third chapter titled “the emphasis on the objects” selects some of these elements in different scales: tower, stairs, skylight, and fountain. In order study in depth these elements, they are compared to other references that pose similar issues in order to understand Fisac’s concepts and scope. Through the tower and the cloister stair, he addresses certain issues in relation to the geometry and structural grid. The skylights raise lighting, acoustic and indoor continuity concepts, and the small stone, wood and metal objects are used in order to carry out an approach to the material and technique through the detail that Fisac applies in almost all his work. With the overlapping of these visions - the historian, the architect and critic - the degree of elaboration is calibrated and conclusions are met to represent the most important aspects of Fisac’s project.

Reducing the impact of EV charging on the electric grid

One of the main objectives of European Commission related to climate and energy is the well-known 20-20-20 targets to be achieved in 2020: Europe has to reduce greenhouse gas emissions of at least 20% below 1990 levels, 20% of EU energy consumption has to come from renewable resources and, finally, a 20% reduction in primary energy use compared with projected levels, has to be achieved by improving energy efficiency. In order to reach these objectives, it is necessary to reduce the overall emissions, mainly in transport (reducing CO2, NOx and other pollutants), and to increase the penetration of the intermittent renewable energy. A high deployment of battery electric (BEVs) and plug-in hybrid electric vehicles (PHEVs), with a low-cost source of energy storage, could help to achieve both targets. Hybrid electric vehicles (HEVs) use a combination of a conventional internal combustion engine (ICE) with one (or more) electric motor. There are different grades of hybridation from micro-hybrids with start-stop capability, mild hybrids (with kinetic energy recovery), medium hybrids (mild hybrids plus energy assist) and full hybrids (medium hybrids plus electric launch capability). These last types of vehicles use a typical battery capacity around 1-2 kWh. Plug in hybrid electric vehicles (PHEVs) use larger battery capacities to achieve limited electric-only driving range. These vehicles are charged by on-board electricity generation or either plugging into electric outlets. Typical battery capacity is around 10 kWh. Battery Electric Vehicles (BEVs) are only driven by electric power and their typical battery capacity is around 15-20 kWh. One type of PHEV, the Extended Range Electric Vehicle (EREV), operates as a BEV until its plug-in battery capacity is depleted; at which point its gasoline engine powers an electric generator to extend the vehicle's range. The charging of PHEVs (including EREVs) and BEVs will have different impacts to the electric grid, depending on the number of vehicles and the start time for charging. Initially, the lecture will start analyzing the electrical power requirements for charging PHEVs-BEVs in Flanders region (Belgium) under different charging scenarios. Secondly and based on an activity-based microsimulation mobility model, an efficient method to reduce this impact will be presented.

A multi-level analysis of innovation diffusion: The case of solar photovoltaic systems in Germany and the disillusion of grid parity

¿Suministrarán las fuentes de energía renovables toda la energía que el mundo necesita algún día? Algunos argumentan que sí, mientras que otros dicen que no. Sin embargo, en algunas regiones del mundo, la producción de electricidad a través de fuentes de energía renovables ya está en una etapa prometedora de desarrollo en la que su costo de generación de electricidad compite con fuentes de electricidad convencionales, como por ejemplo la paridad de red. Este logro ha sido respaldado por el aumento de la eficiencia de la tecnología, la reducción de los costos de producción y, sobre todo, los años de intervenciones políticas de apoyo financiero. La difusión de los sistemas solares fotovoltaicos (PV) en Alemania es un ejemplo relevante. Alemania no sólo es el país líder en términos de capacidad instalada de sistemas fotovoltaicos (PV) en todo el mundo, sino también uno de los países pioneros donde la paridad de red se ha logrado recientemente. No obstante, podría haber una nube en el horizonte. La tasa de difusión ha comenzado a declinar en muchas regiones. Además, las empresas solares locales – que se sabe son importantes impulsores de la difusión – han comenzado a enfrentar dificultades para manejar sus negocios. Estos acontecimientos plantean algunas preguntas importantes: ¿Es ésta una disminución temporal en la difusión? ¿Los adoptantes continuarán instalando sistemas fotovoltaicos? ¿Qué pasa con los modelos de negocio de las empresas solares locales? Con base en el caso de los sistemas fotovoltaicos en Alemania a través de un análisis multinivel y dos revisiones literarias complementarias, esta tesis doctoral extiende el debate proporcionando riqueza múltiple de datos empíricos en un conocimiento de contexto limitado. El primer análisis se basa en la perspectiva del adoptante, que explora el nivel "micro" y el proceso social que subyace a la adopción de los sistemas fotovoltaicos. El segundo análisis es una perspectiva a nivel de empresa, que explora los modelos de negocio de las empresas y sus roles impulsores en la difusión de los sistemas fotovoltaicos. El tercero análisis es una perspectiva regional, la cual explora el nivel "meso", el proceso social que subyace a la adopción de sistemas fotovoltaicos y sus técnicas de modelado. Los resultados incluyen implicaciones tanto para académicos como políticos, no sólo sobre las innovaciones en energía renovable relativas a la paridad de red, sino también, de manera inductiva, sobre las innovaciones ambientales impulsadas por las políticas que logren la competitividad de costes. ABSTRACT Will renewable energy sources supply all of the world energy needs one day? Some argue yes, while others say no. However, in some regions of the world, the electricity production through renewable energy sources is already at a promising stage of development at which their electricity generation costs compete with conventional electricity sources’, i.e., grid parity. This achievement has been underpinned by the increase of technology efficiency, reduction of production costs and, above all, years of policy interventions of providing financial support. The diffusion of solar photovoltaic (PV) systems in Germany is an important frontrunner case in point. Germany is not only the top country in terms of installed PV systems’ capacity worldwide but also one of the pioneer countries where the grid parity has recently been achieved. However, there might be a cloud on the horizon. The diffusion rate has started to decline in many regions. In addition, local solar firms – which are known to be important drivers of diffusion – have started to face difficulties to run their businesses. These developments raise some important questions: Is this a temporary decline on diffusion? Will adopters continue to install PV systems? What about the business models of the local solar firms? Based on the case of PV systems in Germany through a multi-level analysis and two complementary literature reviews, this PhD Dissertation extends the debate by providing multiple wealth of empirical details in a context-limited knowledge. The first analysis is based on the adopter perspective, which explores the “micro” level and the social process underlying the adoption of PV systems. The second one is a firm-level perspective, which explores the business models of firms and their driving roles in diffusion of PV systems. The third one is a regional perspective, which explores the “meso” level, i.e., the social process underlying the adoption of PV systems and its modeling techniques. The results include implications for both scholars and policymakers, not only about renewable energy innovations at grid parity, but also in an inductive manner, about policy-driven environmental innovations that achieve the cost competiveness.

Selection, training and validation process of a sensory panel for bread analysis: Influence of cultivar on the quality of breads made from common wheat and spelta wheat

A protocol of selection, training and validation of the members of the panel for bread sensory analysis is proposed to assess the influence of wheat cultivar on the sensory quality of bread. Three cultivars of bread wheat and two cultivars of spelt wheat organically-grown under the same edaphoclimatic conditions were milled and baked using the same milling and baking procedure. Through the use of triangle tests, differences were identified between the five breads. Significant differences were found between the spelt breads and those made with bread wheat for the attributes ?crumb cell homogeneity? and ?crumb elasticity?. Significant differences were also found for the odor and flavor attributes, with the bread made with ?Espelta Navarra? being the most complex, from a sensory point of view. Based on the results of this study, we propose that sensory properties should be considered as breeding criteria for future work on genetic improvement.