Synthesis and structures of oxyanion encapsulated copper(I)-dppm complexes (dppm = bis(diphenylphosphino) methane)

Copper(I)-dppm complexes encapsulating the oxyanions ClO4-, NO3-, CH3C6H4CO2-, SO42-, and WO42- have been synthesized either by reduction of the corresponding Cu(II) salts and treatment with dppm, or by treating the complex [Cu-2(dppm)(2)(dmcn)(3)](BF4)(2) (1) (dmcn = dimethyl cyanamide) with the respective anion. The isolated complexes [Cu-2(dppm)(2)(dmcn)(2)(ClO4)] (ClO4) (2), [Cu-2(dppm)(2)(dmcn)(2)(NO3)] (NO3) (3), Cu-2(dppm)(2)(NO3)(2) (4), [Cu-2(dppm)(2)(CH3C6H4CO2)(2)]dmcn.2THF (5), Cu-2(dppm)(2)(SO4) (6), and [Cu-3(dppm)(3)(Cl)(WO4)] 0.5H(2)O (7) have been characterized by IR, H-1 and P-31{H-1} NMR, UV-vis, and emission spectroscopy. The solid-state molecular structure of complexes 1, 2, 4, and 7 were determined by single-crystal X-ray diffraction. Pertinent crystal data are as follows: for 1, monoclinic P2(1)/c, a = 11.376(10) Angstrom, b = 42.503(7) Angstrom, c = 13.530(6) Angstrom, beta = 108.08(2)degrees, V = 6219(3) Angstrom(3), Z = 4; for 2, monoclinic P2(1)/c, a = 21.600(3) Angstrom, b = 12.968(3) Angstrom, c = 23.050(3) Angstrom, beta = 115.97(2)degrees, V = 5804(17) Angstrom(3), Z = 4; for 4, triclinic , a = 10.560(4) Angstrom, b = 10.553(3) Angstrom, c = 22.698(3) Angstrom, alpha = 96.08(2)degrees, beta = 96.03(2)degrees, gamma = 108.31(2)degrees, V = 2362(12) Angstrom(3), Z = 2; and for 7, orthorhombic P2(1)2(1)2(1), a = 14.407(4) Angstrom, b = 20.573(7) Angstrom, c = 24.176(6) Angstrom, V = 7166(4) Angstrom(3), Z = 4. Analyses of the crystallographic and spectroscopic data of these complexes reveal the nature of interactions between the Cu-I-dppm core and oxyanion. The anchoring of the oxyanion to the Cu-n(dppm)(n) unit is primarily through coordination to the metal, but the noncovalent C-H ... O interactions between the methylene and phenyl protons of the dppm and oxygen atoms of the oxyanion play a significant role. The solid-state emission spectra for complexes 1-6 are very similar but different from 7. In CDCl3 solution, addition of ClO4- or NO3- (as their tetrabutylammonium salts) to 1 establishes a rapid equilibrium between the anion-complexed and uncomplexed forms. The association constant values for ClO4- and NO3- have been estimated from the P-31{H-1} NMR spectra.

Grain Floatation During Equiaxed Solidification of an Al-Cu Alloy in a Side-Cooled Cavity: Part I-Experimental Studies

Experimental studies were performed to investigate the role and influence of grain movement on macrosegregation and microstructure evolution during equiaxed solidification. Casting experiments were performed with a grain-refined Al-Cu alloy in a rectangular sand mold. For the aluminum alloy studied, the equiaxed grains are lighter than the bulk melt and thus float up. Experiments were designed to investigate floatation phenomena of equiaxed grains in the presence of thermosolutal convection. Cooling curves were recorded at key locations in both the casting and the chill. Quantitative image analysis and spatial chemical analysis were performed on the solidified casting to observe the chemical and microstructural inhomogeneity created by the melt convection and solid floatation. Several notable features that can be attributed to grain movement were observed in temperature histories, macrosegregation patterns, and microstructures. In our experiments, the floatation of grains influences the thermal conditions and the overall flow direction in the casting cavity. In some cases, the induced flow resulting from the grain movement caused a flow reversal. This in turn influences the solidification direction, microstructure evolution, and the overall macrosegregation behavior.

Deciphering complex patterns of class-I HLA-peptide cross-reactivity via hierarchical grouping

T-cell responses in humans are initiated by the binding of a peptide antigen to a human leukocyte antigen (HLA) molecule. The peptide-HLA complex then recruits an appropriate T cell, leading to cell-mediated immunity. More than 2000 HLA class-I alleles are known in humans, and they vary only in their peptide-binding grooves. The polymorphism they exhibit enables them to bind a wide range of peptide antigens from diverse sources. HLA molecules and peptides present a complex molecular recognition pattern, as many peptides bind to a given allele and a given peptide can be recognized by many alleles. A powerful grouping scheme that not only provides an insightful classification, but is also capable of dissecting the physicochemical basis of recognition specificity is necessary to address this complexity. We present a hierarchical classification of 2010 class-I alleles by using a systematic divisive clustering method. All-pair distances of alleles were obtained by comparing binding pockets in the structural models. By varying the similarity thresholds, a multilevel classification was obtained, with 7 supergroups, each further subclassifying to yield 72 groups. An independent clustering performed based only on similarities in their epitope pools correlated highly with pocket-based clustering. Physicochemical feature combinations that best explain the basis of clustering are identified. Mutual information calculated for the set of peptide ligands enables identification of binding site residues contributing to peptide specificity. The grouping of HLA molecules achieved here will be useful for rational vaccine design, understanding disease susceptibilities and predicting risk of organ transplants.

Cybernetic Modeling Revisited: A Method for Inferring the Cybernetic Variables u(i) from Experimental Data

The cybernetic modeling framework for the growth of microorganisms provides for an elegant methodology to account for the unknown regulatory phenomena through the use of cybernetic variables for enzyme induction and activity. In this paper, we revisit the assumption of limited resources for enzyme induction (Sigma u(i) = 1) used in the cybernetic modeling framework by presenting a methodology for inferring the individual cybernetic variables u(i) from experimental data. We use this methodology to infer u(i) during the simultaneous consumption of glycerol and lactose by Escherichia coli and then model the fitness trade-offs involved in the recently discovered predictive regulation strategy of microorganisms.

Evaluación de niveles de adopción de prácticas en el cultivo de café (Coffea arabica L.) en la cuarta Región

En la zona cafetalera de la IV Región de Nicaragua, ubicada en la Costa del Océano Pacifico se realizó entre los meses de noviembre de 1995 y 1996; una encuesta estructurada con el objetivo de evaluar los niveles de adopción de prácticas por parte de los productores de café, recomendadas por el departamento de extensión de UNICAFE. En la Región (dividida en 3 zonas) se recomendaron prácticas para el cultivo de café en función de dar respuesta a siete aspectos (problemas irritantes) que según el diagnóstico tecnológico realizado por UNICAFE a los Productores entre Octubre y Diciembre de 1994 son los que más inciden en la reducción de la productividad del cultivo, siendo los siguientes: Manejo de tejido (poda), manejo de malezas, Fertilización, Manejo y Regulación de sombra, control de plagas, muestreo de suelo y control de enfermedades. Para realizar la encuesta se analizaron productores cafetaleros en tres zonas específicas de la IV Región (zonificación de acuerdo a la organización del programa de extensión de la UNICAFE). La zona I representa el Mombacho, la UNICAFE tiene siete Grandes Productores beneficiarios, seleccionando al azar tres como muestra de la zona que represento el 42.9% del universo. Aquí se adoptaron en un 65.5% las practicas. La zona II, representa a Masatepe y anexos, la UNICAFE tiene 21 Productores beneficiarios, seleccionando al azar 12 como muestra de la zona que represento el 57.1%. Aquí los Grandes Productores (1) adopto en un 57.1% las practicas, los Mediano (7) adoptaron en un 81.7% y los pequeños (4) adoptaron en un 82.1%. La adopción total en esta zona fue de 79.8%. La zona III, representa a Carazo y la Concepción, la UNICAFE cuenta con 39 productores beneficiarios, seleccionando al azar 12 como muestra de la zona que represento el 30.8%. Aquí los Grandes Productores (3) adoptaron en un 91.7% las practicas, los Medianos (5) adoptaron en un 85.4% y los Pequeños (3) adoptaron en un 91.2%. La adopción total en esta zona fue de 88.4%. Los pequeños productores presentaron el mayor nivel de adopción en la región con 86.0%, los Medianos Productores obtuvieron un 83.4% y los Grandes Productores obtuvieron el más bajo nivel de adopción con un 76.5%.Las practicas con mayor porcentaje de adopción fueron las dirigidas al control de enfermedades con 93.0%, siendo adoptada en un 100% por 22 productores de la Región. Las practicas menos adoptadas fueron las dirigidas a la fertilización con 54.3%. La adopción total de las prácticas en la región fue de 82.0%. En general la no adopción de las prácticas se atribuye principalmente a la falta de recursos económicos y la falta de tiempo, la primera debido a la falta de habilitaciones bancarias y la segunda por la falta de personal para llevar a cabo las prácticas. En la región los rendimientos aumentaron de 539.44 kg oro/ha (8.34 qq oro/mz) ciclo 94-95 a 634.52 kg oro/ha (9.81 qq oro/mz) ciclo 95-96, para un 17.63% de incremento y la producción aumento de 494,033.25 kg oro (7,637.9 qq oro) ciclo 94-95 a 617,695.16 kg oro (9,549.75 qq oro) ciclo 95-96. Para aumento de 25.03%.

La participación de la mujer y los niños en el manejo integrado de plagas en las regiones I y II de Nicaragua

El presente trabajo es el resultado de un diagnostico realizado en las regiones I y II de Nicaragua. El objetivo del estudio fue conocer el rol que juegan las mujeres y los niños en las prácticas de control de plagas y en particular en el manejo de los plaguicidas. Para obtener la información del estudio se realizó una encuesta dirigida a mujeres y niños en 18 comunidades de los departamentos de Estelí, Nueva Segovia, León y Chinandega. En el estudio resultó que la participación de la mujer y los menores es alta, los resultados muestran que el 96% de la mujer y el 85% de los niños tienen participación en todas las fases agrícolas. Las prácticas de manejo integrado de plagas implementadas por las productoras son mayoritariamente recuentos de plagas, rotación de cultivos, cultivos en asocio y variedades mejoradas. Generalmente la aplicación de plaguicidas, lavado de la ropa contaminada, preparación de la mezcla y acarreo del plaguicida al lugar de aplicación. En el manejo y exposición al riesgo de los plaguicidas el 65% de las productoras en la región II contestó que conocían de personas intoxicadas, además identificaron la intoxicación laboral como la más común. En la región I, 42% de las productoras mencionó conocer de personas intoxicadas. Cuatro de los plaguicidas reportados en existencia por las agricultoras son altamente a extremadamente peligrosos, estos plaguicidas son almacenados principalmente dentro de la casa, 15% de las productoras utilizan los envases de plaguicidas para almacenar agua y alimento y el 8% para echar más veneno. Además el 88% de las productoras mencionaron conocer de los efectos adversos de la utilización de los plaguicidas, sobre todo en la salud humana. Finalmente la encuesta indica que las capacitaciones en MIP son dirigidas en su gran mayoría a los hombres y cuando incluyen a las mujeres los lugares y hora escogida para las capacitaciones eran uno los factores que obstaculizaba su participación. La falta de títulos de propiedad, que limita el acceso al crédito para la siembra, fue otro factor limitante para participar en actividades MIP. Las instituciones y los programas radiales educativos, así como la comunicación directa, juegan un papel importante en la transmisión de conocimientos MIP

Dynamic mode I perturbation solution for a moving crack unsteadily

Rice et al. (Jounal of Mechanics and Physics of Solids 42, 813-843) analyze the propagation of a planar crack with a nominally straight front in a model elastic solid with a single displacement component. Using the form of Willis er al. (Journal of the Mechanics and Physics of Solids 43, 319-341), of dynamic mode I weight functions for a moving crack, we address that problem solved by Rice ei al. in the 3D context of elastodynamic theory. Oscillatory crack tip motion results from constructive-destructive interference of stress intensity waves. Those waves, including system of the dilatational, shear and Rayleigh waves, interact on each other and with moving edge of crack, can lead to continuing fluctuations of the crack front and propagation velocity. (C) 1997 Elsevier Science Ltd.

Efecto de la edad de destete y del sistema de alimentación sobre el crecimiento en terneras de lechería

Se efectuó una prueba de alimentación usando el diseño de parcela dividida con un arreglo completamente al azar, con 36 terneras provenientes del cruce Holstein y Pardo Suizo, para comparar el efecto de dos edades de destete 4 y 8 semanas, y tres sistemas de alimentación en el crecimiento de terneras de lecheria. Los tratamientos fueron los siguientes: a) leche integra suministrada en forma gradual y el destete a las 4 semanas; b) leche integra suministrada en forma constante y el destete a las 4 semanas; c) sustituto de leche por 4 semanas; d, e y f, similares a los tratamientos anteriores en lo referente al alimento liquido pero con destete a las 8 semanas. El resto de la racion consistio en alimentos concentrados; iniciador, desarrollo y heno. Desde las 9 a las 20 semanas las terneras pasaron en grupos de seis a corrales comunes. El iniciador suministrado a las terneras de los tratamientos a y d se ofreció a razón de 144 gr. por litro de leche integra, previa disolución en agua, y el iniciador que consumieron las terneras de los tratamientos c y f se administro a razón de 0.90 kg hasta el destete. El concentrado de desarrollo se ofreció a los tratamientos a, b, d y a donde la 1 a las 20 semanas y en los tratamientos c y f después del destete hasta alcanzar un máximo de 2.7 kg. Todos los tratamientos tuvieron a disposición a partir del destete heno de estrella (Cynodon sp). La ganancia de peso vivo promedio total, de 66.48 kg obtenida por las terneras del tratamiento e, fue similar a las de 56.84, 50.74, 53.72 y 55.53 kg logradas por las terneras de los tratamientos b, c,d y f, pero significativamente diferente (P<0.05), a 43.42 kg alcanzada por las terneras del tratamiento a. El promedio de ganancias de peso vivo por día desde la 1 hasta las 20 semanas de edad fue 0.31, 0.41, 0.36, 0.38, 0.47 y 0.40 kg para los tratamientos a al f respectivamente. La mayor ganancia promedio en altura a la cruz 20.32 cm lograda por las terneras del tratamiento II fue similar a la de 17.97 cm obtenida por las terneras del tratamiento III, pero significativamente diferentes (P<0.01), con la de 15.94 cm alcanzada por las terneras del sistema I. El promedio de ganancia en perímetro toraxico fue 22.23, 27.30, 26.29, 26.29, 30.35 y 26.29 cm para los tratamientos a al f respectivamente. Estas ganancias no fueron significativas (P<0.05). Los costos totales de alimentación por ternera desde la 1 hasta las 20 semanas fueron 245.98, 315.74, 283.41, 318.41, 457.48 y 335.33 córdobas para los tratamientos a al f respectivamente. Los costos correspondientes por kg de aumento en peso vivo, fueron 5.67, 5.55, 5.59, 5.93, 6.88 y 6.04 respectivamente.

Aquaculture Asia, Vol. 10, No. 4, pp.1-42, October-December 2005

*Table of Contents* Sustainable aquaculture Peter Edwards writes on rural aquaculture: Small-scale pond culture in Bangladesh. People in aquaculture Community based aquaculture - issues and challenges H.K. De and G.S. Saha. Aquaculture as an action programme: An exercise in building confidence and self worth. B. Shanthi, V.S. Chandrasekaran, M. Kailasam, M. Muralidar, T. Ravisankar,.C. Saradad and M. Krishnan The STREAM Column: Transforming policy recommendations into pro-poor service provision Graham Haylor. Research & farming techniques. Grow out of juvenile spotted Babylon to marketable size in earthen ponds II: Polyculture with seabass. S. Kritsanapuntu, N. Chaitanawisuti, W. Santhaweesuk and Y. Natsukari Asia-Pacific Marine Finfish Aquaculture Network. Influence of economic conditions of importing nations and unforeseen global events on grouper markets. Sih Yang Sim. Present status of hatchery technology for cobia in Vietnam. Nhu Van Can. Report on grouper hatchery training course in Indonesia. Nguyen Quoc Thai. Aquatic animal health. Biosecured and improved penaeid shrimp production through organic nursery raceway system in India. Felix. S. and M. Samaya Kannan. Management of monogenean parasites in brackishwater finfish. K.P. Jithendran, M. Natarajan and I.S. Azad. Vembanad Lake: A potential spawner bank of the giant freshwater prawn Macrobrachium rosenbergii on the southwest coast of India. Paramaraj Balamurugan, Pitchaimuthu Mariappan & Chellam Balasundaram.

Characterization of the benthos, marine debris and bottom fish at Gray’s Reef National Marine Sanctuary

Executive Summary: Baseline characterization of resources is an essential part of marine protected area (MPA) management and is critical to inform adaptive management. Gray’s Reef National Marine Sanctuary (GRNMS) currently lacks adequate characterization of several key resources as identified in the 2006 Final Management Plan. The objectives of this characterization were to fulfill this need by characterizing the bottom fish, benthic features, marine debris, and the relationships among them for the different bottom types within the sanctuary: ledges, sparse live bottom, rippled sand, and flat sand. Particular attention was given to characterizing the different ledge types, their fish communities, and the marine debris associated with them given the importance of this bottom type to the sanctuary. The characterization has been divided into four sections. Section 1 provides a brief overview of the project, its relevance to sanctuary needs, methods of site selection, and general field procedures. Section 2 provides the survey methods, results, discussion, and recommendations for monitoring specific to the benthic characterization. Section 3 describes the characterization of marine debris. Section 4 is specific to the characterization of bottom fish. Field surveys were conducted during August 2004, May 2005, and August 2005. A total of 179 surveys were completed over ledge bottom (n=92), sparse live bottom (n=51), flat sand (n=20), and rippled sand (n=16). There were three components to each field survey: fish counting, benthic assessment, and quantification of marine debris. All components occurred within a 25 x 4 m belt transect. Two divers performed the transect at each survey site. One diver was responsible for identification of fish species, size, and abundance using a visual survey. The second diver was responsible for characterization of benthic features using five randomly placed 1 m2 quadrats, measuring ledge height and other benthic structures, and quantifying marine debris within the entire transect. GRNMS is composed of four main bottom types: flat sand, rippled sand, sparsely colonized live bottom, and densely colonized live bottom (ledges). Independent evaluation of the thematic accuracy of the GRNMS benthic map produced by Kendall et al. (2005) revealed high overall accuracy (93%). Most discrepancies between map and diver classification occurred during August 2004 and likely can be attributed to several factors, including actual map or diver errors, and changes in the bottom type due to physical forces. The four bottom types have distinct physical and biological characteristics. Flat and rippled sand bottom types were composed primarily of sand substrate and secondarily shell rubble. Flat sand and rippled sand bottom types were characterized by low percent cover (0-2%) of benthic organisms at all sites. Although the sand bottom types were largely devoid of epifauna, numerous burrows indicate the presence of infaunal organisms. Sparse live bottom and ledges were colonized by macroalgae and numerous invertebrates, including coral, gorgonians, sponges, and “other” benthic species (such as tunicates, anemones, and bryozoans). Ledges and sparse live bottom were similar in terms of diversity (H’) given the level of classification used here. However, percent cover of benthic species, with the exception of gorgonians, was significantly greater on ledge than on sparse live bottom. Percent biotic cover at sparse live bottom ranged from 0.7-26.3%, but was greater than 10% at only 7 out of 51 sites. Colonization on sparse live bottom is likely inhibited by shifting sands, as most sites were covered in a layer of sediment up to several centimeters thick. On ledge bottom type, percent cover ranged from 0.42-100%, with the highest percent cover at ledges in the central and south-central region of GRNMS. Biotic cover on ledges is influenced by local ledge characteristics. Cluster analysis of ledge dimensions (total height, undercut height, undercut width) resulted in three main categories of ledges, which were classified as short, medium, and tall. Median total percent cover was 97.6%, 75.1%, and 17.7% on tall, medium, and short ledges, respectively. Total percent cover and cover of macroalgae, sponges, and other organisms was significantly lower on short ledges compared to medium and tall ledges, but did not vary significantly between medium and tall ledges. Like sparse live bottom, short ledges may be susceptible to burial by sand, however the results indicate that ledge height may only be important to a certain threshold. There are likely other factors not considered here that also influence spatial distribution and community structure (e.g., small scale complexity, ocean currents, differential settlement patterns, and biological interactions). GRNMS is a popular site for recreational fishing and boating, and there has been increased concern about the accumulation of debris in the sanctuary and potential effects on sanctuary resources. Understanding the types, abundance, and distribution of debris is essential to improving debris removal and education efforts. Approximately two-thirds of all observed debris items found during the field surveys were fishing gear, and about half of the fishing related debris was monofilament fishing line. Other fishing related debris included leaders and spear gun parts, and non-gear debris included cans, bottles, and rope. The spatial distribution of debris was concentrated in the center of the sanctuary and was most frequently associated with ledges rather than at other bottom types. Several factors may contribute to this observation. Ledges are often targeted by fishermen due to the association of recreationally important fish species with this bottom type. In addition, ledges are structurally complex and are often densely colonized by biota, providing numerous places for debris to become stuck or entangled. Analysis of observed boat locations indicated that higher boat activity, which is an indication of fishing, occurs in the center of the sanctuary. On ledges, the presence and abundance of debris was significantly related to observed boat density and physiographic features including ledge height, ledge area, and percent cover. While it is likely that most fishing related debris originates from boats inside the sanctuary, preliminary investigation of ocean current data indicate that currents may influence the distribution and local retention of more mobile items. Fish communities at GRNMS are closely linked to benthic habitats. A list of species encountered, probability of occurrence, abundance, and biomass by habitat is provided. Species richness, diversity, composition, abundance, and biomass of fish all showed striking differences depending on bottom type with ledges showing the highest values of nearly all metrics. Species membership was distinctly separated by bottom type as well, although very short, sparsely colonized ledges often had a similar community composition to that of sparse live bottom. Analysis of fish communities at ledges alone indicated that species richness and total abundance of fish were positively related to total percent cover of sessile invertebrates and ledge height. Either ledge attribute was sufficient to result in high abundance or species richness of fish. Fish diversity (H`) was negatively correlated with undercut height due to schools of fish species that utilize ledge undercuts such as Pareques species. Concurrent analysis of ledge types and fish communities indicated that there are five distinct combinations of ledge type and species assemblage. These include, 1) short ledges with little or no undercut that lacked many of the undercut associated species except Urophycis earlii ; 2) tall, heavily colonized, deeply undercut ledges typically with Archosargus probatocephalus, Mycteroperca sp., and Pareques sp.; 3) tall, heavily colonized but less undercut with high occurrence of Lagodon rhomboides and Balistes capriscus; 4) short, heavily colonized ledges typically with Centropristis ocyurus, Halichoeres caudalis, and Stenotomus sp.; and 5) tall, heavily colonized, less undercut typically with Archosargus probatocephalus, Caranx crysos and Seriola sp.. Higher levels of boating activity and presumably fishing pressure did not appear to influence species composition or abundance at the community level although individual species appeared affected. These results indicate that merely knowing the basic characteristics of a ledge such as total height, undercut width, and percent cover of sessile invertebrates would allow good prediction of not only species richness and abundance of fish but also which particular fish species assemblages are likely to occur there. Comparisons with prior studies indicate some major changes in the fish community at GRNMS over the last two decades although the causes of the changes are unknown. Species of interest to recreational fishermen including Centropristis striata, Mycteroperca microlepis, and Mycteroperca phenax were examined in relation to bottom features, areas of assumed high versus low fishing pressure, and spatial dispersion. Both Mycteroperca species were found more frequently when undercut height of ledges was taller. They often were found together in small mixed species groups at ledges in the north central and southwest central regions of the sanctuary. Both had lower mode size and proportion of fish above the fishery size limit in heavily fished areas of the sanctuary (i.e. high boat density) despite the presence of better habitat in that region. Black sea bass, C. striata, occurred at 98% of the ledges surveyed and appeared to be evenly distributed throughout the sanctuary. Abundance was best explained by a positive relationship with percent cover of sessile biota but was also negatively related to presence of either Mycteroperca species. This may be due to predation by the Mycteroperca species or avoidance of sites where they are present by C. striata. Suggestions for monitoring bottom features, marine debris, and bottom fish at GRNMS are provided at the end of each chapter. The present assessment has established quantitative baseline characteristics of many of the key resources and use issues at GRNMS. The methods can be used as a model for future assessments to track the trajectory of GRNMS resources. Belt transects are ideally suited to providing efficient and quantitative assessment of bottom features, debris, and fish at GRNMS. The limited visibility, sensitivity of sessile biota, and linear nature of ledge habitats greatly diminish the utility of other sampling techniques. Ledges should receive the bulk of future characterization effort due to their importance to the sanctuary and high variability in physical structure, benthic composition, and fish assemblages. (PDF contains 107 pages.)

Galeria dos brasileiros illustres (os contemporaneos) : retratos dos homens mais illustres do Brasil na politica, sciencias e letras, desde a guerra da independencia até os nossos dias : copiados do natural e lithographados por S.A. Sisson, acompanhados das suas respectivas biographias, publicada sob a protecção de sua Magestade o Imperador

Galeria dos brasileiros illustres traz retratos copiados do natural e litografados das principais figuras brasileiras da política, ciências e letras, com biografias redigidas por diversos escritos da época, por alguns dos próprios biografados ou por membros de suas famílias. Considerada a mais importante de Sisson, esta obra teve tiragem reduzida e muitos exemplares foram desmanchados por antiquários que emolduravam os retratos, vendendo-os por alto preço. Segundo Borba de Moraes, "é muito difícil encontrar-se hoje em dia um exemplar perfeito e em boas condições" e que "só muito raramente aparece um exemplar à venda."

Evaluation of the body burdens of heavy metals in five commercially important fishes from different locations in Kainji Lake

The level and distribution of some heavy metals viz Cadmium, Lead, Copper Zinc, and Cobalt in five commercially important fishes, water and sediments at three different locations in Kainj Lake were determined using standard methods. The results show that the ranges of heavy metals mu g/g in fishes in Dam site Laotian are: Cd (0.05~c0.01-20~c01), (Pb(ND-1.12 plus or minus )1), Cu (0.81~c25-2.93~c06), Zn (20.89 arrow right .15-36.78~c2.97), Co(0.08~c01-0.27~c02); in cover Dam, the ranges are Cd (0.04~c02-0.16~c0.2), Pb (nd-02~c01), Cu(0.75~c05-2.61~c13), Zn(15.70~c1.55-32.23~c2.70), Co(0.04~c02-0.25~c0.01) and in Yuna they are Cd (0.05~c01-0.14~c02), Pb (nd-0.32~c01), Cu (0.23~c07-2.70~c05), Zn(15.50 plus or minus `.35-25.62~c2.47), Co(0.07~c02-23~c0.01). The metals concentration (mg/l) in the water sample from Dam site, cover dam and Yuna respectively are Cd(0.007~c001,. 004~c001 and 0.005~001), Pb(013~c001, ND and ND), Cu(.055~c008.030~c007, 05 plus or minus .010), Zn(0.13~c01, 0.060 plus or minus .0055) and Co (.026 plus or minus .022 plus or minus .004, .024 plus or minus .004), while the metals concentration ( mu g/g) in sediments sample from Dam site, cover dam and Yuna are respectively Cd(.05 plus or minus .01, .02 plus or minus .01), Pb(16.00~c1.00, ND and 9.33~c1.01), Cu(24.00~c1.34, 4.26 plus or minus .91 and 11.08~c1.32), Zn(42.00~c1.00, 35~c10 and 38.00 plus or minus .45), Co(15.00~c1.17, 8.69~c1.21 and 10.91~c44). The concentrations of the tested heavy metals are within the acceptable standards of WHO (1987a)

I. The crystal structure of trimesic acid. II. Topics in crystallographic calculations

I. Trimesic acid (1, 3, 5-benzenetricarboxylic acid) crystallizes with a monoclinic unit cell of dimensions a = 26.52 A, b = 16.42 A, c = 26.55 A, and β = 91.53° with 48 molecules /unit cell. Extinctions indicated a space group of Cc or C2/c; a satisfactory structure was obtained in the latter with 6 molecules/asymmetric unit - C₅₄O₃₆H₃₆ with a formula weight of 1261 g. Of approximately 12,000 independent reflections within the CuK_α sphere, intensities of 11,563 were recorded visually from equi-inclination Weissenberg photographs.

The structure was solved by packing considerations aided by molecular transforms and two- and three-dimensional Patterson functions. Hydrogen positions were found on difference maps. A total of 978 parameters were refined by least squares; these included hydrogen parameters and anisotropic temperature factors for the C and O atoms. The final R factor was 0.0675; the final "goodness of fit" was 1.49. All calculations were carried out on the Caltech IBM 7040-7094 computer using the CRYRM Crystallographic Computing System.

The six independent molecules fall into two groups of three nearly parallel molecules. All molecules are connected by carboxylto- carboxyl hydrogen bond pairs to form a continuous array of sixmolecule rings with a chicken-wire appearance. These arrays bend to assume two orientations, forming pleated sheets. Arrays in different orientations interpenetrate - three molecules in one orientation passing through the holes of three parallel arrays in the alternate orientation - to produce a completely interlocking network. One third of the carboxyl hydrogen atoms were found to be disordered.

II. Optical transforms as related to x-ray diffraction patterns are discussed with reference to the theory of Fraunhofer diffraction.

The use of a systems approach in crystallographic computing is discussed with special emphasis on the way in which this has been done at the California Institute of Technology.

An efficient manner of calculating Fourier and Patterson maps on a digital computer is presented. Expressions for the calculation of to-scale maps for standard sections and for general-plane sections are developed; space-group-specific expressions in a form suitable for computers are given for all space groups except the hexagonal ones.

Expressions for the calculation of settings for an Eulerian-cradle diffractometer are developed for both the general triclinic case and the orthogonal case.

Photographic materials on pp. 4, 6, 10, and 20 are essential and will not reproduce clearly on Xerox copies. Photographic copies should be ordered.

Development of Microfluidic Devices with the Use of Nanotechnology to Aid in the Analysis of Biological Systems Including Membrane Protein Separation, Single Cell Analysis, and Genetic Markers

Computation technology has dramatically changed the world around us; you can hardly find an area where cell phones have not saturated the market, yet there is a significant lack of breakthroughs in the development to integrate the computer with biological environments. This is largely the result of the incompatibility of the materials used in both environments; biological environments and experiments tend to need aqueous environments. To help aid in these development chemists, engineers, physicists and biologists have begun to develop microfluidics to help bridge this divide. Unfortunately, the microfluidic devices required large external support equipment to run the device. This thesis presents a series of several microfluidic methods that can help integrate engineering and biology by exploiting nanotechnology to help push the field of microfluidics back to its intended purpose, small integrated biological and electrical devices. I demonstrate this goal by developing different methods and devices to (1) separate membrane bound proteins with the use of microfluidics, (2) use optical technology to make fiber optic cables into protein sensors, (3) generate new fluidic devices using semiconductor material to manipulate single cells, and (4) develop a new genetic microfluidic based diagnostic assay that works with current PCR methodology to provide faster and cheaper results. All of these methods and systems can be used as components to build a self-contained biomedical device.

The role of infusoria and bacteria as food for Cyclopoida in Rybinsk Reservoir [Translation from: Trudy Instituta Biologii Vnutrennykh Vodnany 22(25), 37-42, 1971]

Despite the fact that it is a long time sinc e the methods of collection and quantitative estimatio n of protozoa were worked out data on the abundance of these animals in freshwaters are not very numerous. Nevertheless, Infusoria and Rhizopoda at high densities are widespread in the water and sediments of lakes and reservoirs. In order to find ou t the importance of infusoria as a food source for cyclopoids we carried out experimental investigations using C14. For this three species of cyclopoids, widely distributed in open regions of Rybinsk reservoir were selected: Cyclops vicinus, Mesocyclops oithonoides and Kesocyclops leuckarti. The data presented give evidence that the species investigated are able as predators to consume infusorian plankton arid bacteria in the form of flocs or small clumps of detritus.