Letter to Charlie Brown from Daniel Denison Whedon

This letter is part of the Autograph Letters Collection.

The study of molecular variation in Atlantic salmon (Salmo salar L.) and brown trout (Salmo trutta L.)

Polymorphic microsatellite DNA loci were used here in three studies, one on Salmo salar and two on S. trutta. In the case of S. salar, the survival of native fish and non-natives from a nearby catchment, and their hybrids, were compared in a freshwater common garden experiment and subsequently in ocean ranching, with parental assignment utilising microsatellites. Overall survival of non-natives was 35% of natives. This differential survival was mainly in the oceanic phase. These results imply a genetic basis and suggest local adaptation can occur in salmonids across relatively small geographic distances which may have important implications for the management of salmon populations. In the first case study with S trutta, the species was investigated throughout its spread as an invasive in Newfoundland, eastern Canada. Genetic investigation confirmed historical records that the majority of introductions were from a Scottish hatchery and provided a clear example of the structure of two expanding waves of spread along coasts, probably by natural straying of anadromous individuals, to the north and south of the point of human introduction. This study showed a clearer example of the genetic anatomy of an invasion than in previous studies with brown trout, and may have implications for the management of invasive species in general. Finally, the genetics of anadromous S. trutta from the Waterville catchment in south western Ireland were studied. Two significantly different population groupings, from tributaries in geographically distinct locations entering the largest lake in the catchment, were identified. These results were then used to assign very large rod caught sea trout individuals (so called “specimen” sea trout) back to region of origin, in a Genetic Stock Identification exercise. This suggested that the majority of these large sea trout originated from one of the two tributary groups. These results are relevant for the understanding of sea trout population dynamics and for the future management of this and other sea trout producing catchments. This thesis has demonstrated new insights into the population structuring of salmonids both between and within catchments. While these chapters look at the existence and scale of genetic variation from different angles, it might be concluded that the overarching message from this thesis should be to highlight the importance of maintaining genetic diversity in salmonid populations as vital for their long-term productivity and resilience.

Prolactin receptor signaling is essential for perinatal brown adipocyte function: a role for insulin-like growth factor-2.

BACKGROUND: The lactogenic hormones prolactin (PRL) and placental lactogens (PL) play central roles in reproduction and mammary development. Their actions are mediated via binding to PRL receptor (PRLR), highly expressed in brown adipose tissue (BAT), yet their impact on adipocyte function and metabolism remains unclear. METHODOLOGY/PRINCIPAL FINDINGS: PRLR knockout (KO) newborn mice were phenotypically characterized in terms of thermoregulation and their BAT differentiation assayed for gene expression studies. Derived brown preadipocyte cell lines were established to evaluate the molecular mechanisms involved in PRL signaling on BAT function. Here, we report that newborn mice lacking PRLR have hypotrophic BAT depots that express low levels of adipocyte nuclear receptor PPARgamma2, its coactivator PGC-1alpha, uncoupling protein 1 (UCP1) and the beta3 adrenoceptor, reducing mouse viability during cold challenge. Immortalized PRLR KO preadipocytes fail to undergo differentiation into mature adipocytes, a defect reversed by reintroduction of PRLR. That the effects of the lactogens in BAT are at least partly mediated by Insulin-like Growth Factor-2 (IGF-2) is supported by: i) a striking reduction in BAT IGF-2 expression in PRLR KO mice and in PRLR-deficient preadipocytes; ii) induction of cellular IGF-2 expression by PRL through JAK2/STAT5 pathway activation; and iii) reversal of defective differentiation in PRLR KO cells by exogenous IGF-2. CONCLUSIONS: Our findings demonstrate that the lactogens act in concert with IGF-2 to control brown adipocyte differentiation and growth. Given the prominent role of brown adipose tissue during the perinatal period, our results identified prolactin receptor signaling as a major player and a potential therapeutic target in protecting newborn mammals against hypothermia.

Revisió del Zygodon viridissimus (Dicks.) R. Brown als herbaris de Barcelona

Con esta nota, siguiendo en parte a SMITH 1978, se rectitica la determinación de Zygodon baumgartneri Malta, identificado como Z. viridissimus (Dicks.) R. Brown en los herbarios BCB y BCF, y llamaremos Z. baumgartneri Malta fo. mediterranea (Malta) comb. nov. a las especies de Z. viridissimus (Dicks.) R. Brown fo. mediterranea Malta.

Bupleurum salicifolium R. Brown in Buch de l'archipel Macaronésien: Taxonomie et Evolution

Se ha realizado un estudio anatómico (frutos y hojas) en 24 poblaciones de Bupleurum salicifolium R. Brown in Buch recogidas en las lslas Canarias Occidentales y Madeira, lo que nos ha permitido evidenciar una estructura uniforme de los frutos. Por el contrario, en las hojas se han observado tres tipos de estructuras, así como la existencia de un canal oleoso, cuya presencia o ausencia coincide con la distribución geográfica de la especie en las diferentes islas. Estas observaciones nos han inducido a hacer una nueva propuesta sistemática: B. salicifolium subsp. salicifolium con dos variedades, var. salicifolium (Madeira y Gomera) y var. robustum comb. nova (Gomera), y subsp. aciphyllum (La Palma, Hierro, Tenerife y Gran Canaria).La importancia de cada uno de los tipos de hojas en las diferentes islas nos ha pemitido asimismo proponer un esquema de dispersión de la especie. La existencia de estructuras consideradas como más primitivas, así como la desaparición de las poblaciones diploides, permiten suponer que B. salicifolium es una especie antigua que conserva su número base de origen x = 8 (En francés).