613 resultados para basil
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Objetivou-se avaliar a atividade antifúngica dos óleos essenciais de Ocimum basilicum L. (manjericão), Cymbopogon martinii L. (palmarosa), Thymus vulgaris L. (tomilho) e Cinnamomum cassia Blume (canela da china) sobre cepas de Candida albicans isoladas de pacientes HIV positivos e cepa padrão (ATCC 76845). Quinze amostras clínicas de C. albicans (C1-C15) foram repicadas em ágar Sabouraud Dextrose, para confecção de suspensões em solução salina estéril (0,9%) contendo 1,5 x 10(6) UFC mL-1. As emulsões dos óleos essenciais foram preparadas em água destilada estéril e tween 80, com concentrações variando entre 1024 µg mL-1 e 4 µg mL-1. A ação antifúngica foi determinada por meio da Concentração Inibitória Mínima (CIM) utilizando-se a técnica da microdiluição. Foram utilizados como controles positivos a nistatina e o miconazol (50 µg mL-1). Os testes foram realizados em triplicata, sendo a CIM, a menor concentração capaz de inibir o crescimento das leveduras, observada por método visual de acordo com a turvação do meio de cultura. Para C. albicans (ATCC 76845), a CIM do óleo essencial de C. cassia foi 64 µg mL-1, enquanto para óleo de C. martinii foi 1024 µg mL-1. Para as cepas clínicas, verificou-se que a CIM de C. cassia para 80% das cepas foi 64 µg mL-1, sendo a variação dos valores da CIM entre 128 µg mL-1 e 64 µg mL-1. Observou-se que para 66,6% das amostras clínicas, a CIM de C. martinii foi 612 µg mL-1. Constatou-se que a nistatina não apresentou atividade frente às cepas clínicas (C1-C15), enquanto a atividade antifúngica do miconazol foi verificada em 100% das amostras. Não se constatou atividade antimicrobiana dos óleos essenciais de O. basilicum e T. vulgaris, nas concentrações avaliadas. Concluiu-se que os óleos essenciais de C. cassia e C. martinii, em diferentes concentrações, apresentam atividade antifúngica sobre cepas de C. albicans isoladas de pacientes HIV positivos e cepa padrão (ATCC 76845). Entretanto não foi observada inibição antimicrobiana para os óleos de O. basilicum e T. vulgaris.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Ocimum basilicum L., popularly known as sweet basil, is a Lamiaceae species whose essential oil is mainly composed of monoterpenes, sesquiterpenes and phenylpropanoids. The contents of these compounds can be affected by abiotic and biotic factors such as infections caused by viruses. The main goal of this research was an investigation of the effects of viral infection on the essential oil profile of common basil. Seeds of O. basilicum L. cv. Genovese were sowed and kept in a greenhouse. Plants presenting two pairs of leaves above the cotyledons were inoculated with an unidentified virus isolated from a field plant showing chlorotic yellow spots and foliar deformation. Essential oils of healthy and infected plants were extracted by hydrodistillation and analyzed by GCMS. Changes in essential oil composition due to viral infection were observed. Methyleugenol and p-cresol,2,6-di-tert-butyl were the main constituents. However, methyleugenol contents were significantly decreased in infected plants.
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Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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O presente texto parte de uma caracterização em que o movimento escolanovista brasileiro é considerado como difusor da introdução de procedimentos científicos no campo pedagógico, como se a educação devesse subordinar-se às regras do pensamento e às descobertas obtidas pela ciência. em seguida, mostra que essa caracterização descreve apenas uma das tendências do ideário educacional renovador, sendo que outra, desenvolvida marcadamente sob a influência de John Dewey, filósofo e educador norte-americano, apresenta norteamentos bastante diversos. Assim, analisa alguns artigos publicados na Revista Brasileira de Estudos Pedagógicos, órgão do Instituto Nacional de Estudos Pedagógicos, INEP, nos anos de 1950. Tais artigos, de autoria de Anísio Teixeira, João Roberto Moreira e Luiz Alves de Mattos, entre outros, traduzem o ideário educacional renovador daquele período e expressam concepções fundamentadas nas idéias de John Dewey. Os temas debatidos por esses educadores brasileiros são agrupados, de maneira geral, em torno das seguintes categorias: democracia, liberdade, pesquisa sócio-educacional e planejamento educacional. Na análise desenvolvida, percebe-se que os autores expressam diferentes visões do mesmo pensador - John Dewey -, fato que é compreensível mediante o conceito de recontextualização, originário das teses de Basil Bernstein, segundo o qual as teorias ou concepções filosóficas sofrem alterações, muitas vezes fundamentais, quando são convertidas em discurso pedagógico. Ao mesmo tempo que espera contribuir para melhor compreensão da presença do pensamento deweyano no Brasil, o presente trabalho também sugere a necessidade de maior aprofundamento quanto ao estudo dos processos recontextualizadores no campo educacional.
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The objective of the experiment was to test attractive substances for Africanized honey bees, Apis mellifera L., in avocado, Persea americana Mill. Were pulverized extracts of lemon grass (Cympobogon citratus), ocimum (Ocimum sellowii), Lippia alba, orange leaves (Citrus sinensis), eucaliptus leaves (Eucalyptus sp), eugenol and linalol and the frequency of bees on flowers were evaluated. The frequency of honey bees did increase with the application of the extracts falsa melissa, eucaliptus leaves and orange leaves, used as attractants in Quintal variety. The products tested in Eppendorf tubes did not increased the frequency the bees in Quintal and Fortuna varieties.
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The microorganism Sclerotinia was isolated from roots of Stevia rebaudiana (Bert.) Bertoni in plantations in the northwest of Parana and submitted to the cultivation in the presence of extracts and vegetable balsams of Tarragon (Artemisia draconculus), Thyme (Thymus vulgaris), Manjerona (Origanum majorona), Mint citrata (Mintpiperita var. citrata), Purple Basil (Ocimum basilicum L.), Andiroba (Carapa guanensis) and Copaíba (Copaifera reticulata Ducke). The first five oils were extracted by steam drags, after the drying of the vegetable in greenhouse with circulation of air at 45°C. The last two were used in natura. A suspension (100ìl) of fungus previously cultivated, was added to each plate. The results show that after 7 days of incubation the thyme oils 10ìl, purple basil 25ìl, manjerona 25ìl, mint citrata 50ìl, tarragon 50ìl were capable to inhibit the growth of Sclerotinia, while the andiroba oil only reached this result with 200ìl. The copaiba balsam, even in the concentration of 500ìl, was unable to inhibit the growth of the microorganism.
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The aim of this experiment was to study the influence of several levels of magnesium on development and essential oil content of basil, plants were cultivated using Hoagland and Arnon (1950) number one 1 complete solution, containing 48.6 mg L -1 and with decreasing levels of magnesium (24.3 mg L -1 and 12.1 mg L -1). The experimental design was completely randomized, in a factorial scheme 3x3, which is, three levels of magnesium and three harvest times, with three replications each. The parameters evaluated were the stem length, leaf dry mass, stem dry mass, roots dry mass, absolute growth rate (AGR), relative growth rate (RGR), net assimilatory rate (NAR), leaf area ratio (LAR), leaf mass ratio (LMR), dry mass distribution on several organs, stem and leaf/root ratio and essential oil yield, all those measured in three harvest time 30, 60 and 90 days after transplantation (DAT). Plants cultivated with the lower levels of magnesium showed no symptoms of deficiency, but showed differences in the parameters studied. Essential oil yield was higher at 30 DAT for plants cultivated with 12.1 mg L -1 the magnesium. Our results suggests that Hoagland and Arnon (1950) no 1 complete solution overestimates the level of magnesium, as has been observed in several other plant species.
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Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
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We report on the results of a double-blind, randomized, controlled clinical trial comparing two preparations of ethinylestradiol and cyproterone acetate in the treatment of women of reproductive age presenting menstrual irregularities of hyper-androgenic origin. After obtaining informed consent, subjects were randomized to a 4-month treatment period consisting of one daily dose of 0.035mg ethinylestradiol + 2mg cyproterone acetate. The treatment regimen cycle consisted of one pill, once daily for 21 days, followed by a 7-day pill-free period. We compared the efficacy of two presentations of the drug combination after each treatment cycle (Visits 2, 3, 4, and 5) in establishment and maintenance of menstrual regulation, intensity of menstrual flow, and dysmenorrhea, as well as a comparison of the two presentations in terms of Global Satisfaction and Drug Satisfaction assessments performed by the patients and the investigating physician. At each study visit, drug compliance and use of concomitant medications, as well as incidence, severity and duration of adverse events were recorded. A total of 86 subjects were randomized to treatment, with 43 subjects in each treatment group. At Visit 2 and each subsequent visit, all patients in both treatment groups reported an episode of withdrawal bleeding during the 7-day hormone-free period. We observed a statistically significant (p<0.0001) decrease in the incidence of dysmenorrhea at each study visit in relation to the pretreatment assessment. There was a significant reduction (p<0.0001) in the number of subjects reporting intermenstrual bleeding at each study visit in both treatment groups. Global Satisfaction scores by the patient and physician increased significantly at each successive study visit in both treatment groups. There were no clinically significant changes in vital signs, weight, and body mass index throughout the study period in either group. The number of subjects reporting adverse events at each visit did not vary between treatment groups. The combined oral contraceptive pill containing ethinylestradiol and cyproterone acetate was found to be both effective and safe in the menstrual irregularities of hyper-androgenic origin (amenorrhea, dysmenorrhea, and intermenstrual bleeding) assessed in this study. © Copyright Moreira Jr. Editora. Todos os direitos reservados.
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Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
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Includes bibliography
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Pós-graduação em Aquicultura - FCAV
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Pós-graduação em Artes - IA