Ação reversora in vitro e in vivo do verapamil sobre a resistência de Haemonchus contortus à invermectina

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)

Atividade endectocida, segurança clínica e farmacocinética de resíduos de uma nova alternativa terapêutica (Fluazuron + Abamectina) em bovinos

Pós-graduação em Medicina Veterinária - FCAV

Janaúba (Himatanthus Willd. Ex. Schult.) – Apocynaceae no controle de nematódeos gastrintestinais em ovinos

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Effect of alternate and simultaneous grazing on endoparasite infection in sheep and cattle

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)

Diagnóstico de resistência anti-helmíntica em bovinos

Pós-graduação em Medicina Veterinária - FMVZ

Sustainable worm control practices in South America

A high prevalence of nematodes, especially Haemonchus contortus and Trichostrongylus colubriformis, that exhibit multiple anthelmintics resistance has been reported in sheep in several South American countries. For this reason, the development of strategies that are less dependent on anthelmintic treatments is imperative for the prophylaxis of gastrointestinal nematode infections in small ruminants. Integrated grazing using cattle and sheep can be used for pasture decontamination with considerable reduction of H. contortus and T. colubnformis infective larvae after cattle grazing. Several breeds of sheep exhibit genetically related resistance against nematode infections, as is the case of crioulo, native or naturalised breeds of sheep. These breeds descend from livestock introduced by Portuguese and Spanish settlers and have been submitted to a long process of natural selection in various environmental conditions. In the South, the Crioula Lanada breed is more resistant to H. contortus than are Corriedale sheep. In tropical areas, where the minimum temperatures are usually higher than 20 degrees C, hair sheep flourish, especially the Santa Ines breed, which also display a higher level of resistance to nematode infections compared with certain breeds of European origin. However, Santa Ines sheep have inferior carcass quality compared with other commercial breeds. Recent studies showed that the crossbreeding of Santa Ines ewes with sires of breeds with high potential for growth and meat production, results in crossbred animals with high productivity and a satisfactory degree of resistance against nematode infections. Several studies have indicated that improvement in nutrition has a beneficial effect on the development of resistance in lambs that were naturally or artificially infected with nematodes. Therefore, supplementary feeding and breeding strategies to improve resistance to nematodes are feasible options in the effort to reduce dependence on anthelmintic drugs to control worm infections in sheep. (C) 2013 Elsevier B.V. All rights reserved.

Assessing resistance of ivermectin and moxidectin against nematodes in cattle naturally infected using three different methodologies

The objective of this study was to evaluate the accuracy of the faecal egg count reduction test (FECRT) and the faecal egg count efficacy test (FECET) to assess the resistance status of ivermectin (630 mu g/g) and moxidectin (200 mu g/kg), using the controlled efficacy test as a reference, and whether the results of the EPG are equivalent to the efficacy results from the parasitological necropsies. Two experiments were conducted. The results demonstrate that it was not possible to demonstrate that the EPG values were equivalent with the ivermectin and moxidectin efficacy obtained by parasitological necropsies, mainly if the phenomenon of parasites resistance is not advanced in a determined field population. Maybe the FECET technique would be possibly better than the FECRT. The high anthelmintic efficacy of 200 mu g/kg moxidectin, in naturally infected cattle, against field population of nematodes that are resistant to 630 mu g/kg ivermectin, was observed in this study. (C) 2013 Elsevier Ltd. All rights reserved.

Resistência anti-helmíntica em equinos na Região Oeste do Estado de São Paulo

Pós-graduação em Ciência e Tecnologia Animal - FEIS

Nanostructured Lipid Carriers as a Strategy to Improve the In Vitro Schistosomiasis Activity of Praziquantel

Praziquantel (PZQ) is a pyrazinoisoquinoline anthelmintic that was discovered in 1972 by Bayer Germany. Currently, due to its efficacy, PZQ is the drug of choice against all species of Schistosoma. Although widely used, PZQ exhibits low and erratic bioavailability because of its poor water solubility. Nanostructured lipid carriers (NLC), second-generation solid lipid nanoparticles, were developed in the 1990s to improve the bioavailability of poorly water soluble drugs. The aim of this study was to investigate nanostructured lipid carriers as a strategy to improve the efficacy. of PZQ in S. mansoni treatment. We prepared NLC2 and NLC4 by adding seventy percent glycerol monostearate (GMS) as the solid lipid, 30% oleic acid (OA) as the liquid lipid and two surfactant systems containing either soybean phosphatidylcholine/poloxamer (PC/P-407) or phosphatidylcholine/Tween 60 (PC/T60), respectively. The carriers were characterized by nuclear magnetic resonance, differential scanning calorimetry, thermogravimetric analysis and Fourier transform-infrared spectroscopy. The safety profile was evaluated using red cell hemolysis and in vitro cytotoxicity assays. The results showed that the encapsulation of PZQ in NLC2 or NLC4 improved the safety profile of the drug. Treatment efficacy was evaluated on the S. mansoni BH strain. PZQ-NLC2 and PZQ-NLC4 demonstrated an improved efficacy in comparison with free PZQ. The results showed that the intestinal transport of free PZQ and PZQ-NLC2 was similar. However, we observed that the concentration of PZQ absorbed was smaller when PZQ was loaded in NLC4. The difference between the amounts of absorbed PZQ could indicate that the presence of T60 in the nanoparticles (NLC4) increased the rigid lipid matrix, prolonging release of the drug. Both systems showed considerable in vitro activity against S. mansoni, suggesting that these systems may be a promising platform for the administration of PZQ for treating schistosomiasis.

Platinosomíase em felinos domésticos: um diferencial para obstrução biliar

The platinosomiase is a disease the hepatobiliary system of domestic felines being caused by trematode Platynosomum fastosum. The life cycle of the parasite includes the presence of three intermediate hosts (snails, terrestrial isopodes, lizards or frogs). The cat is considered definitive host of the parasite and acquires the infection eating a vertebrate intermediate host containing metacercariae. The adult parasites generally inhabit the liver, gallbladder and bile ducts of cats. The severity of clinical manifestations depends mainly on the number of flukes in the biliary tract. Many animals are asymptomatic or exhibit nonspecific clinical manifestations such as anorexia, lethargy, weight loss, vomiting and diarrhea. In large infestations can occur biliary obstruction resulting in jaundice, cirrhosis, hepatitis cholangiohepatitis and even death. The definitive diagnosis is accomplished through by the detection of fluke eggs in feces or through laparotomy and liver biopsy. Treatment should be based on the use of anthelmintic for the elimination of the parasite and supportive therapy for the animal. Prevention is difficult due to predatory nature of the cat. The control can be accomplished through periodic fecal examinations

Estudo dos compostos voláteis da decomposição térmica dos anti-helmínticos Albendazol e Mebendazol

The albendazole and mebendazole drugs are benzimidazole derivatives and belong to the anthelmintic class. These drugs are particularly recommended for the treatment against worms present in the gastrointestinal tract of animals and humans, by acting directly on the worm metabolism. The need for thermally study drugs is related to all the parameters that these analyzes include: presence or absence of polymorphs, possible changes in the crystallinity of the drugs, as well as the quality control during the manufacturing process thereof. In this study the thermal behavior of anthelmintic albendazole and commercial mebendazole and its recrystallisation in organic solvents, such as acetic acid and formic acid in dimethylformamide to mebendazole, and albendazole were studied using TG-DSC techniques, TG-FTIR, FTIR and XRD. TG-DSC techniques were used so it could collect information about the thermal stability of the compounds steps for thermal decomposition process and also prove its melting temperature. For recrystallization of drugs in organic solvents, the TG-DSC curves were analyzed to compare and determine that the occurrence of polymorphs. The coupled TG-FTIR technique allowed the analysis of volatile products which were released during the thermal decomposition of the commercial mebendazole. The absorption spectroscopy in the infrared region was performed to mebendazole, and albendazole in order to show the difference in functional groups of both, comparing the spectra with commercial drugs and see if there was recrystallized changes in the absorption band where the drug was recrystallized or when heated. The diffraction technique by powder X-ray method was used for comparison of the crystal structures of commercial drugs and recrystallization in organic solvents to identify changes in crystallinity both, which might suggest the formation of polymorphs

Dinâmica populacional de Rhipicephalus (Boophilus) microplus (Canestrini, 1887) em bovinos mestiços mantidos em pastagens de Brachiaria decumbens no município de Formiga, Minas Gerais

Pós-graduação em Medicina Veterinária - FCAV

Estudo epidemiologico do complexo teníase-cisticercose: nova abordagem

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

The effect of gastrointestinal nematode infection level on grazing distance from dung

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)