Multiphoton fluorescence excitation: new spectral windows for biological nonlinear microscopy.

Intrinsic, three-dimensionally resolved, microscopic imaging of dynamical structures and biochemical processes in living preparations has been realized by nonlinear laser scanning fluorescence microscopy. The search for useful two-photon and three-photon excitation spectra, motivated by the emergence of nonlinear microscopy as a powerful biophysical instrument, has now discovered a virtual artist's palette of chemical indicators, fluorescent markers, and native biological fluorophores, including NADH, flavins, and green fluorescent proteins, that are applicable to living biological preparations. More than 25 two-photon excitation spectra of ultraviolet and visible absorbing molecules reveal useful cross sections, some conveniently blue-shifted, for near-infrared absorption. Measurements of three-photon fluorophore excitation spectra now define alternative windows at relatively benign wavelengths to excite deeper ultraviolet fluorophores. The inherent optical sectioning capability of nonlinear excitation provides three-dimensional resolution for imaging and avoids out-of-focus background and photodamage. Here, the measured nonlinear excitation spectra and their photophysical characteristics that empower nonlinear laser microscopy for biological imaging are described.

WINDOWS 10 IoT SU RASPBERRY PI 2: MULTI-PARADIGM PROGRAMMING TRA JAVA E C#

Questa tesi ha come obiettivo la sperimentazione del nuovo sistema operativo Windows 10 IoT Core su tecnologia Raspberry Pi 2, verificandone la compatibilita con alcuni sensori in commercio. Tale studio viene poi applicato in un contesto di Home Intelligence al fine di creare un agente per la gestione di luci LED, in prospettiva della sua integrazione nel sistema prototipale Home Manager.

American flower-garden directory : containing practical directions for the culture of plants in the flower-garden, hot-house, green-house, rooms, or parlour windows, for every month of the year .../

Includes index.

American flower-garden directory : containing practical directions for the culture of plants in the flower-garden, hot-house, green-house, rooms, or parlour windows, for every month of the year .../

Includes index.

An analysis of Gothick architecture : illustrated by a series of upwards of seven hundred examples of doorways, windows, etc., and accompanied with remarks on the several details of an ecclesiastical ediface /

Vol. 2, plates only.

Examples of ancient doorways and windows, arranged to illustrate the different styles of church architecture from the Conquest to the Reformation, from existing examples /

Sheet divided into 9 sections and mounted on cloth. Bound in red bookcloth, stamped in blind and gilt.

Census of Scotland - 1861 : population tables and report : number of the inhabitants, families, children at school, houses, and rooms with windows, in the civil counties and parishes, registration counties and districts, burghs, towns, villages, and islands of Scotland : also a classification of families according to their sizes, the number of persons they contain, and their relative house accommodation : presented by command of Her Majesty to both houses of parliament.

"Errata": 1 p. atend.

Windows to the origins : the Office of Naval Research

Mode of access: Internet.

Economic evaluation of windows in buildings : methodology /

Includes bibliographical references (p. 95-96).

Western windows and other poems.

Mode of access: Internet.