Modernización y cambio social en Bizkaia durante el siglo industrial, 1877-1975. Claves de un proceso

375 p.

Imprensa e poder: discursos e projetos políticos de Francisco Salles Torres Homem (1840-1849)

O presente trabalho procura analisar os discursos de Francisco de Salles Torres Homem, produzidos na imprensa entre 1840 e 1849, momento em que fez parte da facção política liberal. No Período Regencial iniciou a sua atuação no jornalismo, e nos primeiros anos do Segundo Reinado, já aparecia como um importante redator de jornais, panfletário e político. Durante a Revolução Liberal de 1842, participou ativamente do conflito, e no período da Revolução Praieira em 1848, e escreveu o seu mais inflamado e comentado panfleto O Libelo do Povo, onde fez duras críticas ao governo, recebendo grande repercussão na imprensa da época. Esta foi considerada pelos seus biógrafos como a sua fase mais revolucionária, seus discursos foram produzidos em momentos específicos de grande debate de ideias, e expressavam as concepções políticas e ideológicas dos liberais. Naquela época, Salles Torres Homem utilizou à palavra impressa, para defender os interesses dos liberais ao poder.

Revisão do gênero Neotropical Gundlachia Pfeiffer, 1849 (Mollusca, Pulmonata, Basommatophora, Planorboidea, Ancylidae): morfologia, distribuição geográfica, sistemática e filogenia

Gundlachia Pfeiffer 1849 é o gênero de molusco de água doce pateliforme com a mais ampla distribuição geográfica dentre os oito gêneros de Ancylidae assinalados para a região Neotropical. A concha pateliforme, considerada uma homoplasia por diversos autores em vários basomatóforos, tem sido historicamente utilizada para definir a unidade da família Ancylidae, apesar dos questionamentos contrários à monofilia do grupo. Dados moleculares confirmaram que "Ancylidae" é um grupo parafilético, principalmente por causa do gênero Burnupia. Os demais gêneros foram incluídos em Ancylinae, dentro de Planorbidae. No entanto, a ausência de todos os gêneros Neotropicais nas filogenias propostas, com base molecular ainda é um assunto que necessita ser investigado. Os principais objetivos desse trabalho foram: (a) revisar as espécies de Gundlachia, através de estudos morfológicos das conchas e partes moles; (b) padronizar as descrições das espécies para iniciar uma análise cladística do gênero Gundlachia; (c) iniciar um estudo de biologia molecular utilizando distintas espécies de representantes de Ancylinae, para verificar as suas interrelações e o provável grupoirmão de Gundlachia; (d) e por fim, construir um mapa de distribuição geográfica. Para isso, examinamos diversas coleções científicas e realizamos coletas nas localidadestipo. A morfologia das conchas foi comparada por análises morfométricas e por microscópio de luz e de varredura. As partes moles dos espécimes foram dissecadas e estudadas sob o microscópio estereoscópico. A análise molecular foi realizada em três espécimes de cada amostra, utilizando os genes da citocromo c oxidase I e o 16S mtDNA. Após o sequenciamento verificamos as distâncias genéticas entre as diferentes espécies de Gundlachia e as suas relações com os outros gêneros por meio do teste Neighbor-joining e Maximum likelihood. Com esse estudo apresentamos a redescrição de algumas espécies de Gundlachia, discutimos a validade de novos táxons descobertos e sequenciamos e analisamos espécies de sete gêneros de Ancylinae que ocorrem na região Neotropical. Com base nesses dados discutimos a monofilia de Gundlachia e o seu provável grupo-irmão. E ainda abordamos a validade dos demais gêneros neotropicais

Analysis of multivariable Smith predictors using MATLAB Containers

Containers are structured m-files which allow `data' and `methods' to be stored persistently. Containers have a user-defined class structure, so that one can have several Containers of the same class, all structurally similar, and there is a mechanism for interaction with Containers in the style of database transactions. The use of MATLAB Containers to analyze multivariable Smith predictors is discussed.

Vespid chemotactic peptide precursor from the wasp, Vespa magnifica (Smith)

Despite the evolutional distance between wasp and amphibian, vespid chemotactic peptide (VCP), an important component of wasp venom, are found sharing remarkable similarities with the temporin antimicrobial peptides (AMPs) from Ranid frog, Amolops loloens

A phospholipase A1 platelet activator from the wasp venom of Vespa magnifica (Smith)

Wasp is an impor tant venomous animal that can induce human fatalities. Aortic thrombosis and cerebral infarction are major clinical symptoms after massive wasp stings but the reason leading to the envenomation manifestation is still not known. In this paper, a toxin protein is purified and characterized by Sephadex G-75 gel filtration, CM-Sephadex C-25 cationic exchange and fast protein liquid chromatography (FPLC) from the venom of the wasp, Vespa magnifica (Smith). This protein, named magnifin, contains phospholipase-like activity and induces platelet aggregation. The cDNA encoding magnifin is cloned from the venom sac cDNA library of the wasp. The predicted protein was deduced from the cDNA with a sequence composed of 337 amino acid residues. Magnifin is very similar to other phospholipase A(1) (PLA(1)), especially to other wasp allergen PLA(1). Magnifin can activate platelet aggregation and induce thrombosis in vivo. The current results proved that PLA(1) in wasp venom could be contributable to aortic thrombosis after massive wasp stings. (c) 2007 Elsevier Ltd. All rights reserved.

On the little known species of threadfin breams Nemipterus mesoprion (Bleeker) and Nemipterus delagoae (Smith)

Among the fish species of the family Nemipteridae, the two little known species, namely, Nemipterus mesoprion (Bleeker) and Nemipterus delagoae (Smith) are recorded from the Cochin waters. Nemipterus mesoprion is a new distributional record from the west coast of India. N. delagoae is described with adequate numbers of specimen for the first time from Indian waters. Moreover, the results of the present study show that the colour of the viscera, the number of pyloric caeca and the gill rakers can also be used for the diagnosis of the species of the genus Nemipterus. The affinity of the above species with other related species of the genus Nemipterus and their geographical distribution are presented.

Genetic diversity study of Caspian brown trout (Salmo trutta caspius Kessler, 1877) population in five rivers in the southern part of Caspian Sea, Iran

For study the genetic diversity of Caspian brown trout population in five rivers in the southern part of Caspian Sea in Iran 182 number generators in the fall and winter of 1390 were collected in Chalus, Sardab Rud, Cheshmeh Kileh, Kargan Rud and Astara rivers. Then about 3-5 g of soft and fresh tissue from the bottom fin fish removed and were fixed in ethanol 96°. Genomic DNA was extracted by using ammonium acetate, then quantity and quality of the extracted DNA were determined by using spectrophotometry and horizontal electrophoresis in 1% agarose gel. The polymerase chain reaction was performed by using 16 SSR primers and sequencing primers (D-Loop) and the quality of PCR products amplified by SSR method were performed by using horizontal electrophoresis in 2% agarose gel. Alleles and their sizes were determined by using vertical electrophoresis in 6% polyacrylamide gel and silver nitrate staining method. Gel images were recorded by gel documentarian, the bands were scored by using Photo- Capt software and statistical analysis was performed by using Gene Alex and Pop Gene software. Also the PCR sequencing products after quality assessment by usinghorizontal electrophoresis in 1.5% agarose gel were purified and sent to South Korea Bioneer Corporation for sequencing. Sequencing was performed by chain termination method and the statistical analysis was performed by using Bio- Edit, Mega, Arlequin and DNA SP software. The SSR method, 5 pairs of primers produced polymorphic bands and the average real and effective number of alleles were calculated 5.60±1.83 and 3.87±1.46 in the Cheshmeh Kileh river and 7.60±1.75 and 5.48±1.32 in the Karganrud river and the mean observed and expected heterozygosity were calculated 0.44 ±0.15 and 0.52 ±0.16 in the Cheshmeh Kileh river and 0.50 ±0.11 and 0.70±0.13 in the Karganrud river. Analysis of Molecular Variance results showed that significant differences in genetic diversity between and within populations and between and within individuals in the studied rivers (P<0.01). The sequencing method identified 35 different haplotype, the highest number of polymorphic position (251) and haplotype (14) were observed in the Chalus river. The highest mean observed number of alleles (2.24±0.48) was calculated in the Sardabrud river, the highest mean observed heterozygosity (1.00±0.03) was calculated in the Chalus river and the highest mean nucleotide diversity (0.13±0.07) was observed in the Sardabrud river and mean haplotype diversity was obtained (1) in three studied rivers. The overall results show that there are no same population of this fish in the studied rivers and Karganrud and Chalus rivers in the SSR and sequencing methods had the highest levels of genetic diversity.