Morphology, sex ratio and gene expression of Day 14 in vivo and in vitro bovine embryos

The present study was designed to compare Day 14 bovine embryos that were produced entirely in vitro using the post-hatching development (PHD) system with in vivo-derived embryos without or with transient PHD culture from Day 7 to Day 14. Embryos on Day 14 were used for sex determination and gene expression analysis of PLAC8, KRT8, CD9, SLC2A1, SLC2A3, PGK1, HSF1, MNSOD, HSP70 and IFNT using real-time quantitative (q) polymerase chain reaction (PCR). First, Day 7 in vivo-and in vitro-produced embryos were subjected to the PHD system. A higher rate of survival was observed for in vitro embryos on Day 14. Comparing Day 14 embryos produced completely in vivo or completely in vitro revealed that the mean size of the former group was greater than that of the latter (10.29±1.83 vs 2.68±0.33mm, respectively). Expression of the HSP70 and SLC2A1 genes was down-and upregulated, respectively, in the in vitro embryos. The present study shows that in vitro embryos cultured in the PHD system are smaller than in vivo embryos and that of the 10 genes analysed, only two were differentially expressed between the two groups. These findings indicate that, owing to the poor survival rate, the PHD system is not reliable for evaluation of in vitro embryo quality. © 2013 CSIRO.

Ontogenia do sistema sensorial de pacu Piaractus mesopotamicus (Holmberg, 1887) (Characidae: Serrasalmidae)

Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Report of the thirty-fifth session. Lima, 5-9 May 2014

Attendance and organization of work .-- Agenda .-- Summary of proceedings .-- Resolutions adopted at the Thirty-Fifth Session of the Commission: 676(XXXV) ECLAC calendar of conferences for the period 2015-2016 .-- 677(XXXV) Regional Conference on Women in Latin America and the Caribbean .-- 678(XXXV) Statistical Conference of the Americas of the Economic Commission for Latin America and the Caribbean .-- 679(XXXV) Support for the work of the Latin American and Caribbean Institute for Economic and Social Planning .-- 680(XXXV) Caribbean Development and Cooperation Committee .-- 681(XXXV) Regional Conference on Population and Development in Latin America and the Caribbean .-- 682(XXXV) Establishment of the Regional Conference on Social Development in Latin America and the Caribbean .-- 683(XXXV) Admission of Sint Maarten as an associate member of the Economic Commission for Latin America and the Caribbean .-- 684(XXXV) Programme of Work and priorities of the Economic Commission for Latin America and the Caribbean for the 2016-2017 biennium .-- 685(XXXV) Activities of the Economic Commission for Latin America and the Caribbean in relation to follow-up to the Millennium Development Goals and implementation of the outcomes of the major United Nations conferences and summits in the economic, social and related fields .-- 686(XXXV) Application of Principle 10 of the Rio Declaration on Environment and Development in Latin America and the Caribbean .-- 687(XXXV) The regional dimension of the post-2015 development agenda .-- 688(XXXV) South-South Cooperation .-- 689(XXXV) Place of the next session .-- 690(XXXV) Lima Resolution .-- 691(XXXV) Ministerial Conference on the Information Society in Latin America and the Caribbean.

Biennial report (2012-2013)

Includes resolutions adopted at the thirty-fifth session of the Commission, held in 2014.

An assessment of the economic impact of climate change on the Agriculture Sector in Jamaica

The agricultural sector‟s contribution to GDP and to exports in Jamaica has been declining with the post-war development process that has led to the differentiation of the economy. In 2010, the sector contributed 5.8% of GDP, and 3% to the exports (of goods), but with 36% of employment, it continues to be a major employer. With a little less than half of the population living in rural communities, agricultural activities, and their linkages with other economic activities, continue to play an important role as a source of livelihoods, and by extension, the economic development of the country. Sugar cane cultivation has, with the exception of a couple of decades in the twentieth century when it was superseded by bananas, dominated the agricultural export sector for centuries as the source of the raw materials for the manufacture of sugar for export. In 2005, sugar cane itself accounted for 6.4% of the sector‟s contribution to GDP, and 52% of the contribution of agricultural exports to GDP. Production for the domestic market has long been the larger subsector, organized around the production of root crops, especially yams, vegetables and condiments. To analyse the potential impact of climate change on the agricultural sector, this study selected three important crops for detailed examination. In particular, the study selected sugar cane because of its overwhelming importance to the export subsector of agriculture, and yam and escallion for both their contribution to the domestic subsector as well as the preeminent role yams and escallion play in the economic activities of the communities in the hills of central Jamaica, and the plains of the southwest respectively. As with other studies in this project, the methodology adopted was to compare the estimated values of output on the SRES A2 and B2 Scenarios with the value of output on a “baseline” Business As Usual (BAU), and then estimate the net benefits of investment in the relevant to climate change for the selected crops. The A2 and B2 Scenarios were constructed by applying forecasts of changes in temperature and precipitation generated by INSMET from ECHAM inspired climate models. The BAU “baseline” was a linear projection of the historical trends of yields for each crop. Linear models of yields were estimated for each crop with particular attention to the influence of the two climate variables – temperature and precipitation. These models were then used to forecast yields up to 2050 (table1). These yields were then used to estimate the value of output of the selected crop, as well as the contribution to overall GDP, on each Scenario. The analysis suggested replanting sugar cane with heat resistant varieties, rehabilitating irrigation systems where they existed, and establishing technologically appropriate irrigation systems where they were not for the three selected crops.

Histologia e ultra-estrutura dos ovários nas castas de alguns meliponíneos: um estudo comparado

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Contribuição à análise do processo de africanização de Apis mellifera (Hymenoptera, apidae): características do desenvolvimento das glândulas de Dufour e de veneno

Pós-graduação em Ciências Biológicas (Biologia Celular e Molecular) - IBRC

Cicatrização intestinal em ratos submetidos à ingestão de etanol

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)