Genotypes, Recombinant Forms, and Variants of Norovirus GII.4 in Gipuzkoa (Basque Country, Spain), 2009-2012

Background: Noroviruses (NoVs) are genetically diverse, with genogroup II-and within it-genotype 4 (GII.4) being the most prevalent cause of acute gastroenteritis worldwide. The aim of this study was to characterize genogroup II NoV causing acute gastroenteritis in the Basque Country (northern Spain) from 2009-2012. Methods: The presence of NoV RNA was investigated by reverse transcriptase-polymerase chain reaction (RT-PCR) in stool specimens from children younger than 15 years old with community-acquired acute gastroenteritis, and from hospitalized adults or elderly residents of nursing homes with acute gastroenteritis. For genotyping, the open reading frames ORF1 (encoding the polymerase) and ORF2 (encoding the major capsid protein) were partially amplified and sequenced. Recombinant strains were confirmed by PCR of the ORF1/ORF2 junction region. Results: NoV was detected in 16.0% (453/2826) of acute gastroenteritis episodes in children younger than 2 years, 9.9% (139/1407) in children from 2 to 14 years, and 35.8% (122/341) in adults. Of 317 NoVs characterized, 313 were genogroup II and four were genogroup I. The GII.4 variants Den Haag-2006b and New Orleans-2009 predominated in 2009 and 2010-2011, respectively. In 2012, the New Orleans-2009 variant was partially replaced by the Sydney-2012 variant (GII.Pe/GII.4) and New Orleans-2009/Sydney-2012 recombinant strains. The predominant capsid genotype in all age groups was GII.4, which was the only genotype detected in outbreaks. The second most frequent genotype was GII.3 (including the recently described recombination GII.P16/GII.3), which was detected almost exclusively in children. Conclusion: Nine different genotypes of NoV genogroup II were detected; among these, intergenotype recombinant strains represented an important part, highlighting the role of recombination in the evolution of NoVs. Detection of new NoV strains, not only GII.4 strains, shortly after their first detection in other parts of the world shows that many NoV strains can spread rapidly.

Entremeios da arte: moda e arte

Este trabalho versa sobre o universo da arte tangenciado pela moda, sua efemeridade, pertencimentos e seus agenciamentos. Nesse contexto, busca-se exemplificar o quanto a moda e a arte estão intrinsecamente ligadas. O ponto de partida é a moda como experiência e expressão coletiva e/ou individual, principalmente em trajes e formas vestimentares, pois são esses que mais forte influência exercem sobre o homem, seu corpo, seu espaço e memória. Localiza-se esse processo de aproximação entre arte e moda no século XX, quando vanguardas romperam com o academicismo clássico, agregando à arte sinestesias de sistemas relacionais. Rejeitam-se intermitências escultóricas e pictóricas para dar lugar a linguagens que interagem com o espectador. Os dados do presente trabalho traçam o panorama da aproximação entre arte e moda, a partir de duas vertentes: a primeira demonstra como a arte busca, no traje, suas sinergias, influenciando-se e apropriando-se da sinestesia proporcionada pela roupa; a segunda, como a moda apropriou-se de elementos da arte, em busca de um reposicionamento como expressão e não somente consumo

Closed G2 forms and special metrics

170 p.

Balanced parametrizations and canonical forms for system identification

This paper contains a review of recent results concerning the parametrization of asymptotically stable linear systems using balanced realizations. Particular emphasis is given on the application of these results to system identification. This work is part of a continuing programme aimed at elucidating the role of balanced realization in system identification.

A Review of the parasitic copepods of fish recorded from Ceylon with description of additional forms

The beginning of our knowledge of the copepods parasitic on fish from Ceylon is due to Bassett-Smith (1898 a) who, in a paper on "Further New Parasitic Copepods found on Fish in the Indo-Tropical Region", included seven species collected at Trincomalee and Colombo. Later in the same year, in a paper on "Some New or Rare Parasitic Copepods from the Indo-Tropical Region", he (Bassett-Smith, 1898 b) included three more species from Ceylon. Soon after, more of these parasites were obtained from Ceylon during Herdmann's investigation of the Pearl Banks. From this collection, one lot consisting of eleven species was described by Thompson and Scott (1903) and a second lot consisting of seven species was described by Wilson (1906). At that stage the number of species recorded from Ceylon made up to a total of twenty-eight and there the matter rested for another quarter of a century until, quite by chance, while collecting marine animals on a reef, Mr Kirtisinghe came across a newly dead half-beak with a learned parasite projecting from its body. Since then, in a number of occasional papers (Kirtisinghe, 1932-35, 1937, 1950, 1956, 1960) he has described thirty-eight more species of parasitic copepods from Ceylon. However, his collection included many more species which were put aside for later attention. In the present paper, while dealing with those forms in his collection which he has not recorded or described earlier, he has put together all the known forms of parasitic copepods of fish from Ceylon. A list of the host fishes with their respective parasitic copepods is also provided, types of new species, at present in the author's private collection, will be deposited in the Fisheries Department, Colombo, Ceylon.