The Siam repository, containing a summary of Asiatic intelligence.

Vol. 1 lacks subtitle.

Fanny and the regent of Siam.

Mode of access: Internet.

A list of lecturers on southeast Asia (Burma, Siam, French Indo-China, Malay Peninsula, Malay Archipelago, Philippines) and adjacent areas.

Pub. by the institute under its earlier name: East Indies Institute of America.

Buddhistische tempelanlagen in Siam.

Provisional Circ record

Efecto sobre el neurodesarrollo y neuroprotección en pez cebra de un extracto polifenólico de huesos de aceituna

Objetivo: Determinar el efecto de un extracto polifenólico de hueso de oliva en el desarrollo del sistema nervioso y frente al daño inducido mediante la neurotoxina ácido kaínico, utilizando como modelo animal el pez cebra. Material y métodos: Se analiza el efecto del extracto a la máxima dosis tolerada (100 mg/ml de polifenoles) sobre la actividad colinèrgica en larvas de pez cebra (72 horas post-fertilización). Se utilizan únicamente huevos fecundados sin anomalías. Se incuban 6 huevos/pocillo en microplaca de 24 pocillos en 2 ml de agua con DMSO (0,1%) a 26 ± 1º C: a) neurodesarrollo: agua (control) y con 100 mg/ml de extracto, como ensayo; b) neuroprotección: agua y ácido kaínico (100 μM) (control) y con 100 mg/ml de extracto (ensayo). Todas las incubaciones por triplicado. A las 72 h se examinan y verifica ausencia de anomalías. Las larvas se homogeneizan y en los sobrenadantes se cuantifica actividad acetilnolinesterasa y concentración proteínas. Resultados: La cantidad de proteína y apreciación morfológica es análoga en todos los ensayos, indicando mismo desarrollo. La acetilcolinesterasa en las larvas de pez, con el extracto polifenólico es del 162,2%(SD 44,2) respecto a controles (100% de actividad) (p < 0,01). Las larvas de pez tratadas con ácido kaínico y extracto polifenólico presentan el 140,1% (SD 22,0) de actividad, respecto a las incubadas únicamente con la neurotoxina (100%) (p < 0,05). Conclusión: Los polifenoles extraídos de los huesos de aceituna producen incremento de actividad colinèrgica durante el neurodesarrollo larvario en el pez cebra y protección frente a la neurotoxina ácido kaínico.

Efecto de la suplementación con alimento vivo en el desempeño productivo del pez disco (Symphysodon aequefaciatus)

Bogotá (Colombia): Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Zootecnia

Indagaci?n de los procesos de inclusi?n de estudiantes en situaci?n de desplazamiento en la instituci?n educativa Alfonso L?pez Pumarejo de Potrerito ? Jamund? - Valle

El siguiente trabajo de grado, es una indagaci?n de los procesos de inclusi?n de estudiantes en situaci?n de desplazamiento en la instituci?n educativa Alfonso L?pez Pumarejo del Corregimiento Potrerito del Municipio de Jamund? en el Departamento del Valle del Cauca, con el objeto de fortalecer desde la Educaci?n Popular estrategias pedag?gicas que le permitan a los docentes trabajar desde un enfoque diferencial con los estudiantes en situaci?n de desplazamiento. Discursos como el de Paulo Freire, Marco Ra?l Mej?a, y otros autores, permiti? hacer un ejercicio de an?lisis que contribuyeron al prop?sito de generar par?metros pedag?gicos acordes a la idea de construir procesos en la escuela para la poblaci?n infantil y juvenil desplazada que, procedente de diversos puntos geogr?ficos del suroccidente colombiano, estudia en la instituci?n. Como orientaci?n metodol?gica se utiliz? la Investigaci?n Acci?n Participaci?n en cuanto se desarroll? la recolecci?n de informaci?n, an?lisis y la formulaci?n de lineamientos

Toxicidad aguda y efecto bioacumulativo del fungicida Tebucconazol en el pez cebra (Danio rerio).

2009

Siam, Indochina and Malaya /

Relief shown by gradient tints, hachures, and spot heights. Depths shown by gradient tints.

Los hilos de lo desconocido: Luzbel, de Gonzalo Arias Páez

Se escoge un texto periférico en el discurso crítico para indagar sobre la pertinencia de lo fantástico en el conjunto de la literatura nacional a partir del análisis de Luzbel, de Gonzalo Arias Páez.

Acuarios o la opción de tener otras mascotas : querido pez

Fondo Margaritainés Restrepo

Proyecto de factibilidad para la producción y comercialización del pez Arawana en el pie de monte llanero (Osteoglossum bicirrhosum)

Bogotá (Colombia): Universidad de La Salle. Facultad de Ciencias Agropecuarias. Programa de Zootecnia

Cryoreservation of alginate-fibrin beads involving bone marrow derived mesenchymal stromal cells by vitrification

Application of cell-–biomaterial systems in regenerative medicine can be facilitated by their successful low temperature preservation. Vitrification, which avoids ice crystal formation by amorphous solidification, is an emerging approach to cryopreservation. Developing vitrification strategy, effective cryopreservation of alginate–fibrin beads with porcine mesenchymal stromal cells has been achieved in this study. The cell–biomaterial constructs were pre-cultured for 20 days before cryopreservation, allowing for cell proliferation and construct stabilization. Ethylene glycol (EG) was employed as the basic cryoprotectant for two equilibration solutions. Successful cryopreservation of the constructs was achieved using vitrification solution composed of penetrating (EG MW 62 Da) and non-penetrating (sucrose MW 342 Da) cryoprotectants. Stepwise procedure of introduction to and removal of cryoprotectants was brief; direct plunging into liquid nitrogen was applied. Cell viability, evaluated by combining live/death staining and confocal laser microscopy, was similar for both control and vitrified cells in the beads. No detectable damage of microstructure of cryopreserved beads was found as shown by scanning electron microscopy. Both osteogenically induced control and vitrified cells in the constructs were equally capable of mineral production and deposition. There was no statistically significant difference in metabolic activity and proliferation between both groups during the entire culture period. Our study leads to the conclusion that the developed cryopreservation protocol allowed to maintain the integrity of the beads while preserving the ability of the pig bone marrow derived mesenchymal stromal cells to proliferate and subsequently differentiate; demonstrating that vitrification is a promising approach for cryopreser-vation of “ready-to-use” cell–biomaterial constructs.

Vitreous cryopreservation of nanofibrous tissue-engineered constructs generated using mesenchymal stromal cells

Development of an effective preservation strategy to fulfill off-the-shelf availability of tissue-engineered constructs (TECs) is demanded for realizing their clinical potential. In this study, the feasibility of vitrification, ice-free cryopreservation, for precultured ready-to-use TECs was evaluated. To prepare the TECs, bone marrow-derived porcine mesenchymal stromal cells (MSCs) were seeded in polycaprolactone-gelatin nanofibrous scaffolds and cultured for 3 weeks before vitrification treatment. The vitrification strategy developed, which involved exposure of the TECs to low concentrations of cryoprotectants followed by a vitrification solution and sterile packaging in a pouch with its subsequent immersion directly into liquid nitrogen, was accomplished within 11min. Stepwise removal of cryoprotectants, after warming in a 38 degrees C water bath, enabled rapid restoration of the TECs. Vitrification did not impair microstructure of the scaffold or cell viability. No significant differences were found between the vitrified and control TECs in cellular metabolic activity and proliferation on matched days and in the trends during 5 weeks of continuous culture postvitrification. Osteogenic differentiation ability in vitrified and control groups was similar. In conclusion, we have developed a time- and cost-efficient cryopreservation method that maintains integrity of the TECs while preserving MSCs viability and metabolic activity, and their ability to differentiate.

Numerical methods for the variable-order fractional advection-diffusion equation with a nonlinear source term

In this paper, we consider a variable-order fractional advection-diffusion equation with a nonlinear source term on a finite domain. Explicit and implicit Euler approximations for the equation are proposed. Stability and convergence of the methods are discussed. Moreover, we also present a fractional method of lines, a matrix transfer technique, and an extrapolation method for the equation. Some numerical examples are given, and the results demonstrate the effectiveness of theoretical analysis.